LOCUS       sequence-240184-        1191 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated activation of human islet enhancer containing 
            T2D-associated variant rs7732130 (ZBED3 GWAS locus).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1191)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 1191)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: lentiSAMv2 rs7732130-guide1
FEATURES             Location/Qualifiers
     source          1..1191
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     24..43
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        140..158
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        210..228
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     promoter        315..526
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             543..590
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 ttttaaaatt atnttttaaa atggactatc atatgcttac cgtaacttga aagtatttcg
       61 atttcttggc tttatatatc ttgtggaaag gacgaaacac cgatgccaca gacaagttac
      121 tcgttttaga gctaggccaa catgaggatc acccatgtct gcagggccta gcaagttaaa
      181 ataaggctag tccgttatca acttggccaa catgaggatc acccatgtct gcagggccaa
      241 gtggcaccga gtcggtgctt tttttggatc ctaggtcttg aaaggagtgg gaattggctc
      301 cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg
      361 ggtcggcaat tgatccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt
      421 cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt
      481 cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggcgt acggccacca
      541 tgaaaaggcc ggcggccacg aaaaaggccg gccaggcaaa aaagaaaaag gacaagaagt
      601 acagcatcgg cctggccatc ggcaccaact ctgtgggctg ggccgtgatc accgacgagt
      661 acaaggtgcc cagcaagaaa ttcaaggtgc tgggcaacac cgaccggcac agcatcaaga
      721 agaacctgat cggagccctg ctgttcgaca gcggcgaaac agccgaggcc acccggctga
      781 agagaaccgc cagaagaaga tacaccagac ggaagaaccg gatctgctat ctgcaagaga
      841 tcttcagcaa cgagatggcc aaggtggacg acagcttctt ccacagactg gaagagtcct
      901 tcctggtgcg agaaggataa taatcacgag cggcacccca tcttcggcaa catcnnggaa
      961 caaggancnn taacacgaga aagtacccca ccatctaccg cctgnnaata gaaactgggg
     1021 gganggcacc ganaggnccg acctgcggnc taatcaaaca gggccctggc tccacttgaa
     1081 tcaactnncc gggagccact ttccggaaca gagggcgacc nggaaccccg aacaacganc
     1141 taattggang aangtttgtt ggtccgacct ggagnnnaan gtacaaanna g
//
