LOCUS       sequence-240186-        1066 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated activation of human islet enhancer containing 
            T2D-associated variant rs7732130 (ZBED3 GWAS locus).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: lentiSAMv2 rs7732130-guide2
FEATURES             Location/Qualifiers
     source          1..1066
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     17..36
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        133..151
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        203..221
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     promoter        308..519
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             536..583
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 tnatnntttt aaaatggact atcatatgct taccgtaact tgaaagtatt tcgatttctt
       61 ggctttatat atcttgtgga aaggacgaaa caccgctgcc agagtaactt gtctggtttt
      121 agagctaggc caacatgagg atcacccatg tctgcagggc ctagcaagtt aaaataaggc
      181 tagtccgtta tcaacttggc caacatgagg atcacccatg tctgcagggc caagtggcac
      241 cgagtcggtg ctttttttgg atcctaggtc ttgaaaggag tgggaattgg ctccggtgcc
      301 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc
      361 aattgatccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac
      421 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg
      481 aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg cgtacggcca ccatgaaaag
      541 gccggcggcc acgaaaaagg ccggccaggc aaaaaagaaa aaggacaaga agtacagcat
      601 cggcctggcc atcggcacca actctgtggg ctgggccgtg atcaccgacg agtacaaggt
      661 gcccagcaag aaattcaagg tgctgggcaa caccgaccgg cacagcatca agaagaacct
      721 gatcggagcc ctgctgttcg acagcggcga aacagccgag gccacccggc tgaagagaac
      781 cgccagaaga agatacacca gacggaagaa ccggatctgc tatctgcaag agatcttcag
      841 caacgagatg gccaaggtgg acgacagctt cttccacaga ctggaagagt ccttcctggt
      901 ggaagaggat aagaagcacg agcggcaccg catcttcggc aacatcgtgg aacgaggtgg
      961 cctaccacnn gatttaccgc accatctacc acctgaagaa gaaactggtt ggacatcacc
     1021 nncnaagccc aacctgcggc tggatntatc gggcccntgg tccacg
//
