LOCUS       sequence-240188-        1234 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated activation of human islet enhancer containing 
            T2D-associated variant rs7732130 (ZBED3 GWAS locus).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1234)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 1234)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: lentiSAMv2 rs7732130-guide3
FEATURES             Location/Qualifiers
     source          1..1234
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     22..41
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        138..156
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        208..226
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     promoter        313..524
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             541..588
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 tttaaattat nttttaaaat ggactatcat atgcttaccg taacttgaaa gtatttcgat
       61 ttcttggctt tatatatctt gtggaaagga cgaaacaccg cacattaagt tagaacacag
      121 gttttagagc taggccaaca tgaggatcac ccatgtctgc agggcctagc aagttaaaat
      181 aaggctagtc cgttatcaac ttggccaaca tgaggatcac ccatgtctgc agggccaagt
      241 ggcaccgagt cggtgctttt tttggatcct aggtcttgaa aggagtggga attggctccg
      301 gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg
      361 tcggcaattg atccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg
      421 tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg
      481 ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggcgtac ggccaccatg
      541 aaaaggccgg cggccacgaa aaaggccggc caggcaaaaa agaaaaagga caagaagtac
      601 agcatcggcc tggccatcgg caccaactct gtgggctggg ccgtgatcac cgacgagtac
      661 aaggtgccca gcaagaaatt caaggtgctg ggcaacaccg accggnncag catcaagaag
      721 aacctgatcg gagccctgct gttcgacagc ggcgagacag ccgaggccac ccggctgaag
      781 agaaccgcca nangaagata caccagacgg gaagaaccng atctgctatc tgcaanagat
      841 cttcagcatc gagatggcca aggtggnncg acagctnctt ccannnactg gaagagtccc
      901 ttcctggtgg aanaggataa gaaacannaa gcggnannng catnnncggg caacatcatg
      961 ggacgaagat ggtcctancn angannnntn acacgannca tccaacagcc tggataacaa
     1021 aagtggatgg gacnggcact caagaaggnc cgaacctgcc gacttgaatt atnntgcgcc
     1081 ntggaccacn ntggatcaag atnnccaggn nncaccattc cgnnaaccga aggnccgacc
     1141 nggagaaccc gagacaacaa gggnacaagg naccaagtag tatntntngc aagctgannn
     1201 cgataannna gannnnannn nggnncanan nnaa
//
