LOCUS       sequence-240192-        1337 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated activation of ZBED3. Catalytically inactive Cas9 
            from S. pyogenes and VP64 with 2A-BlastR..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 1337)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: lentiSAMv2 ZBED3-TSS-guide2
FEATURES             Location/Qualifiers
     source          1..1337
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        83..101
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        153..171
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     promoter        258..469
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             486..533
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 tnnnnntttn ttgnnttttt atatcttgtg gaaggacgaa acaccggcga gcgacagatt
       61 tgtgtgtttt agagctaggc caacatgagg atcacccatg tctgcagggc ctagcaagtt
      121 aaaataaggc tagtccgtta tcaacttggc caacatgagg atcacccatg tctgcagggc
      181 caagtggcac cgagtcggtg ctttttttgg atcctaggtc ttgaaaggag tgggaattgg
      241 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
      301 aggggtcggc aattgatccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
      361 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
      421 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg cgtacggcca
      481 ccatgaaaag gccggcggcc acgaaaaagg ccggccaggc aaaaaagaaa aaggacaaga
      541 agtacagcat cggcctggcc atcggcacca actctgtggg ctgggccgtg atcaccgacg
      601 agtacaaggt gcccagcaag aaattcaagg tgctgggcaa caccgaccgg cacagcatca
      661 agaagaacct gatcggagcc ctgctgttcg acagcggcga aacagccgag gccacccggc
      721 tgaagagaac cgccagaaga agatacacca gacggaagaa ccggatctgc tatctgcaag
      781 agatcttcag caacgagatg gccaaggtgg acgacagctt cttccacaga ctggaagagt
      841 ccttcctggt ggaagaggat aagaagcacg agcggcaccc catcttcggc aacatcgtgg
      901 acgaggtggc ctaccacgag aagtacccca ccatctacca cctgagaaag aaactggtgg
      961 acagcaccga caaggccgac ctgcggctga tctatctggc cctggcccac atgatcaagt
     1021 tccggggcca cttcctgatc gagggcgacc tgaaccccga caacagcgac gtggacaagc
     1081 tgttcatcca gctggtgcag acctacaacc agctgttcga ggaaaacccc atcaacgcca
     1141 gcggcgggga acgccaaggc catcctgtct gccagactga acaagaacaa acggctggaa
     1201 aatctgatcg cccaactgcc cggcaagaaa aaaaagggcn gttcggnaac cggattgccc
     1261 ggaaccgggg ccggaccccn atttcaaaaa aacttccaac cgggccaaaa agccaaaggg
     1321 nncngnaaag ggaccct
//
