LOCUS       sequence-240196-        1415 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated activation of human islet enhancer containing 
            T2D-associated variant rs58692659 (ZFAND3 GWAS locus).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1415)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 1415)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: lentiSAMv2 rs58692659-guide1
FEATURES             Location/Qualifiers
     source          1..1415
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     34..53
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        149..167
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        219..237
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     promoter        324..535
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             552..599
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 tnnnnnnttg nngttttaat tatgttttaa atggactatc atatgcttac cgtaacttga
       61 aagtatttcg atttcttggc tttatatatc ttgtggaaag gacgaaacac cgttctcccg
      121 gctcaactgc cgttttagag ctaggccaac atgaggatca cccatgtctg cagggcctag
      181 caagttaaaa taaggctagt ccgttatcaa cttggccaac atgaggatca cccatgtctg
      241 cagggccaag tggcaccgag tcggtgcttt ttttggatcc taggtcttga aaggagtggg
      301 aattggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt
      361 ggggggaggg gtcggcaatt gatccggtgc ctagagaagg tggcgcgggg taaactggga
      421 aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag
      481 tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggcgta
      541 cggccaccat gaaaaggccg gcggccacga aaaaggccgg ccaggcaaaa aagaaaaagg
      601 acaagaagta cagcatcggc ctggccatcg gcaccaactc tgtgggctgg gccgtgatca
      661 ccgacgagta caaggtgccc agcaagaaat tcaaggtgct gggcaacacc gaccggcaca
      721 gcatcaagaa gaacctgatc ggagccctgc tgttcgacag cggcgaaaca gccgaggcca
      781 cccggctgaa gagaaccgcc agaagaagat acaccagacg gaagaaccgg atctgctatc
      841 tgcaagagat cttcagcaac gagatggcca aggtggacga cagcttcttc cacagactgg
      901 aagagtcctt cctggtggaa gaggataaga agcacgagcg gcaccccatc ttcggcaaca
      961 tcgtggacga ggtggcctac cacgaaaagt accccaccat ctaccacctg agaaagaaac
     1021 tgggtggaca gcaccgacaa ggcccgacct gcgggctgat ctatctgggc cctgggccna
     1081 antgaatcaa gtttcngtgg ccactttcct ggatcgaagg gnaactgaac ccccgaacaa
     1141 caggggacgt ggacaagcct gtttcatcag gctggngggc agaacttaca acccgtctgg
     1201 ttcgaaggaa aatccccatt caacgcctgg cgggggtgga aagcccaagg gcccattntn
     1261 tcttgcccaa aactggaaac aaaaaagcaa aaatggtttg gaaaaattct taattccccc
     1321 cactttgccc cggtgaaaaa anaaataaaa tgggccctgg tttccggtna cctttgattt
     1381 gccccccgaa nnntcgggnn nnttnannnt cccat
//
