LOCUS       sequence-240198-        1223 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated activation of human islet enhancer containing 
            T2D-associated variant rs58692659 (ZFAND3 GWAS locus).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1223)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 1223)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: lentiSAMv2 rs58692659-guide2
FEATURES             Location/Qualifiers
     source          1..1223
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        115..133
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        185..203
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     promoter        290..501
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             518..565
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 antatnatat gcttaccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
       61 ggaaaggacg aaacaccgat atgggcaggc agttgccgtt ttagagctag gccaacatga
      121 ggatcaccca tgtctgcagg gcctagcaag ttaaaataag gctagtccgt tatcaacttg
      181 gccaacatga ggatcaccca tgtctgcagg gccaagtggc accgagtcgg tgcttttttt
      241 ggatcctagg tcttgaaagg agtgggaatt ggctccggtg cccgtcagtg ggcagagcgc
      301 acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgatc cggtgcctag
      361 agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc
      421 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac
      481 gggtttgccg ccagaacaca ggcgtacggc caccatgaaa aggccggcgg ccacgaaaaa
      541 ggccggccag gcaaaaaaga aaaaggacaa gaagtacagc atcggcctgg ccatcggcac
      601 caactctgtg ggctgggccg tgatcaccga cgagtacaag gtgcccagca agaaattcaa
      661 ggtgctgggc aacaccgacc ggcacagcat caagaagaac ctgatcggag ccctgctgtt
      721 cgacagcggc gaaacagccg aggccacccg gctgaagaga accgccagaa gaagatacac
      781 cagacggaag aaccggatct gctatctgca agagatcttc agcaacgaga tggccaaggt
      841 ggacgacagc ttcttccaca gactggaaga gtccttcctg gtggaagagg ataagaagca
      901 cgagcggcac cccatcttcg gcaacatcgt ggacgaggtg gcctaccacg agaagtaccc
      961 caccatctac cacctgagaa agaaactggg ggacagcacc gacaaggccc gacctgcggc
     1021 tgatctatct ggccctggcc cacatgaatc aagttccggg ggccacttcc tgatcnaagg
     1081 gcgacctgaa ccccgataaa cggcgangtg gaacaagctg ttccatccag ctggnngcaa
     1141 acctacaccc agttggttca aggaaaaacc ccctcaatcc nnngngnctt ggaaccctaa
     1201 ggnnnttnct tgtttngccn tat
//
