LOCUS       sequence-240202-        1222 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated activation of human islet enhancer containing 
            T2D-associated variant rs58692659 (ZFAND3 GWAS locus).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 1222)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: lentiSAMv2 rs58692659-guide4
FEATURES             Location/Qualifiers
     source          1..1222
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     18..37
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        134..152
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        204..222
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     promoter        309..519
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             536..583
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 attatnnttt taaaatggac tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct
       61 tggctttata tatcttgtgg aaaggacgaa acaccgcagt tgccaggcag ttgagcgttt
      121 tagagctagg ccaacatgag gatcacccat gtctgcaggg cctagcaagt taaaataagg
      181 ctagtccgtt atcaacttgg ccaacatgag gatcacccat gtctgcaggg ccaagtggca
      241 ccgagtcggt gctttttttg gatcctaggt cttgaaagga gtgggaattg gctccggtgc
      301 ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg
      361 caattgatcc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta
      421 ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt
      481 gaacgttctt tttcgcaacg ggtttgccgt ngaacacagg cgtacggcca ccatgaaaag
      541 gccggcggcc acgaaaaagg ccggccaggc aaaaaagaaa aaggacaaga agtacagcat
      601 cggcctggcc atcggcacca actctgtggg ctgggccgtg atcaccgacg agtacaaggt
      661 gcccagcaag aaattcaagg tgctgggcaa caccgaccgg cacagcatca agaagaacct
      721 gatcggagcc ctgctgttcg acagcggcga aacagccgag gccacccggc tgaagagaac
      781 cgccagaaga agatacacca gacggaagaa ccggatctgc tatctgcaag agatcttcag
      841 caacgagatg gccaaggtgg acgacagctt cttccacaga ctggaagagt ccttcctggt
      901 ggaagaggat aagaagcacg agcggcaccc catcttcggc aacatcgtgg gacgaggtgg
      961 gcctaccacg agaaagtacc cccaccatct accaccctga gaaaagaaac tgggtggaca
     1021 gccacgaaca agggccaacc tgcggctgga tctatctggg ccctgggccc acatggatca
     1081 agtttccggg gtcnntttcc tggatcgaag gggcaacctg aaaccccgga aaacagcgaa
     1141 ctgggaacaa gcttgtttca atccaacttg gtggcnnaac ntanatccta gctgtttcgn
     1201 nggnaaantc ctccttnnnn nc
//
