LOCUS       sequence-240206-        1436 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated activation of ZFAND3. Catalytically inactive Cas9 
            from S. pyogenes and VP64 with 2A-BlastR..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1436)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 1436)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: lentiSAMv2 ZFAND3-guide2
FEATURES             Location/Qualifiers
     source          1..1436
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     23..42
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        138..156
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        208..226
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     promoter        313..524
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             541..588
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 ttttaaatta tnttttaaaa tggactatca tatgcttacc gtaacttgaa agtatttcga
       61 tttcttggct ttatatatct tgtggaaagg acgaaacacc ggggacacag acgccctcgg
      121 gttttagagc taggccaaca tgaggatcac ccatgtctgc agggcctagc aagttaaaat
      181 aaggctagtc cgttatcaac ttggccaaca tgaggatcac ccatgtctgc agggccaagt
      241 ggcaccgagt cggtgctttt tttggatcct aggtcttgaa aggagtggga attggctccg
      301 gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg
      361 tcggcaattg atccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg
      421 tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg
      481 ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggcgtac ggccaccatg
      541 aaaaggccgg cggccacgaa aaaggccggc caggcaaaaa agaaaaagga caagaagtac
      601 agcatcggcc tggccatcgg caccaactct gtgggctggg ccgtgatcac cgacgagtac
      661 aaggtgccca gcaagaaatt caaggtgctg ggcaacaccg accggcacag catcaagaag
      721 aacctgatcg gagccctgct gttcgacagc ggcgaaacag ccgaggccac ccggctgaag
      781 agaaccgcca gaagaagata caccagacgg aagaaccgga tctgctatct gcaagagatc
      841 ttcagcaacg agatggccaa ggtggacgac agcttcttcc acagactgga agagtccttc
      901 ctggtggaag aggataagaa gcacgagcgg caccccatct tcggcaacat cgtggaacga
      961 gnngccctac cacgagaagt accccaccat ctaccacctg aaaaagaaaa ctggtggaca
     1021 gcaacganan ggcgaacctg cgggctgatc tatctgggcc ntggcccnnn tgatcaagtt
     1081 tccgggggcc acttccttga tcgaaggcca acctgaaccc cgaacaacag gcgacgtgga
     1141 acaannctgt tcnntccagc tggnngcana cctaccncna ncctgtttcg aggaagaacc
     1201 ccattgaagg cccnncgcng ngngaanccc aaggcccatc ctgtcctgcc caaanngaag
     1261 caaaaannng acnggnntgg aaaatcctta tttccccacn tttccaacca agaaaaaaga
     1321 atggaccnan tgtcnnanna cggaagattg ccgaanaccn ggaagcctga agccaccaga
     1381 nnttaaagaa agaaagtttg gaacgttcgg cggaaggatt gccaagcggn ccgntg
//
