LOCUS       sequence-240211-       10820 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  protein expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10820)
  TITLE     Petr Svoboda lab unpublished
REFERENCE   2  (bases 1 to 10820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pEF1-MH.Bl-mDcrSOM
FEATURES             Location/Qualifiers
     source          1..10820
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        67..1249
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          298..1240
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1197..1217
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     1281..1300
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1281..1299
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7068..7097
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             7113..7130
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7153..7170)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7159..7383
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     7724..7743
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7863..8261
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    8419..8540
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8456..8475)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8510..8529
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(8665..9253)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8745..8764)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9424..10215,10216..10284))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10047..10066
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10292..10396)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      join(10423..10820,1..58)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10510..10529)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10720..10741
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttac
       61 gcgtgaggct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag
      121 ttggggggag gggtcggcaa ttgaaccggt gcctagagaa agtggcgcgg ggtaaactgg
      181 gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata
      241 agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta
      301 agtgccgtgt gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct
      361 tgaattactt ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg
      421 aagtgggtgg gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt
      481 tgaggcctgg cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg
      541 tctcgctgct ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct
      601 ttttttctgg caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt
      661 ttttggggcc gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg
      721 gggcctgcga gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc
      781 tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg
      841 gtcggcacca gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc
      901 aaaatggagg acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag
      961 ggcctttccg tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag
     1021 gcacctcgat tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt
     1081 ttatgcgatg gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca
     1141 cttgatgtaa ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa
     1201 gcctcagaca gtggttcaaa gtttttttct tccatttcag gtgtcgtgac gcgtcccact
     1261 gcttactggc ttatcgaaat taatacgact cactataggg agacccaagc tggctagtta
     1321 agcttaccat ggcaggcctg cagctcatga cccctgcttc ctcaccaatg ggtcctttct
     1381 ttggactgcc atggcaacaa gaagcaattc atgataacat ttatacgcca agaaaatacc
     1441 aggttgagct gctggaagca gctctggacc ataacacaat tgtgtgcttg aacactggct
     1501 cagggaagac gttcatcgcg gtcctgctca ccaaagagct ggcccatcag atcaggggcg
     1561 acctcaaccc gcatgcaaaa aggaccgtgt tcctcgtcaa ctctgcaaac caggttgctc
     1621 aacaagtgtc agccgtcaga actcactcgg atctcaaggt tggagagtac tcagacctag
     1681 aagtaaatgc atcttggaca aaagagagat ggagccaaga gtttactaag caccaggttc
     1741 tcattatgac ttgctatgtc gccttgactg ttttgaaaaa tggttactta tcactgtcag
     1801 acattaacct tttggtgttt gatgagtgtc atcttgcgat tctagaccac ccctatcgtg
     1861 aaattatgaa gctctgtgag agttgtccat catgccctcg aattttggga ctaactgctt
     1921 ccattttaaa tgggaaatgt gatccagagg aattggaaga gaagattcag aaactggagc
     1981 gaattctcag gagtgacgct gagacggcga ctgacttggt ggtcctggac agatacactt
     2041 ctcagccttg tgagattgtg gtagactgtg gaccgtttac tgacagaagt gggctgtatg
     2101 agagattgct gatggagtta gaagcagcac ttgattttat caatgattgt aatgtagctg
     2161 tacattcaaa ggaaagagat tctactttga tttccaaaca aatcctctca gactgccgtg
     2221 cagtgttggt tgttctggga ccctggtgtg cagataaagt agctgggatg atggtaagag
     2281 aacttcagaa gtacatcaaa catgagcaag aggagctaca caggaagttc ctattgttta
     2341 cagacacctt gttaaggaaa atacacgccc tgtgtgaaga gtacttctca cctgcctcac
     2401 ttgacctgaa gtatgtaact cccaaagtca tgaaactgct cgagatcctg cgcaaataca
     2461 agccctatga gcgacagcag tttgaaagtg ttgagtggta caataacagg aatcaggata
     2521 attacgtgtc ctggagtgat tccgaggatg acgatgatga cgaagaaatt gaagaaaaag
     2581 aaaagccaga gacaaatttt ccttctccgt ttaccaatat tttatgtggg attatttttg
     2641 tggaaagaag atacacagca gttgtcctaa acagattgat aaaggaagct ggcaaacaag
     2701 atccagagct ggcttatatc agcagcaact ttataactgg acacggcatt ggaaagaacc
     2761 agcctcgcag caaacagatg gaggcggagt tcagaaagca ggaggaggta cttaggaaat
     2821 ttcgagctca tgagaccaac ctgctcattg caacaagtgt tgtagaagag ggtgtggata
     2881 ttcccaagtg caatttggtg gttcgttttg atttgcctac agagtatcga tcatatgtcc
     2941 agtctaaagg acgggcacga gcgccgatct ctaattacgt aatgttagca gatacagaca
     3001 agataaagag ctttgaagaa gacctcaaga cctacaaagc tattgaaaag atcttaagga
     3061 acaagtgttc gaagtcagcg gatggtgctg aggcggacgt ccatgctggt gtagatgatg
     3121 aggatgcttt cccgccatat gtgctgaggc ctgatgatgg gggtcctcga gtcacaatca
     3181 acactgccat tggacacatc aacagatatt gtgctcgatt accaagtgat ccgtttacgc
     3241 atctagctcc caaatgtaga acccgagaat tgcctgatgg tacattttat tcaactcttt
     3301 atcttccgat taactcacct cttcgagcct ccattgttgg tccaccgatg gacagtgtac
     3361 ggttggctga aagagtggta gctctcattt gctgtgaaaa actgcacaaa attggtgaac
     3421 tggatgaaca cttgatgcca gttgggaaag agactgttaa atatgaagaa gagctggact
     3481 tacatgacga ggaggagacc agtgttcccg ggagaccagg gtccacaaaa cgacggcagt
     3541 gctaccccaa agcaattcca gagtgcttga gggagagtta ccccaaaccc gaccagcctt
     3601 gttacctgta tgtgatagga atggtcttaa ctacccccct ccctgatgaa ctcaacttca
     3661 ggcggcggaa gctctaccca cctgaggaca ccacaagatg ctttgggatc ctgacagcca
     3721 aacccatacc tcagattcct cactttcctg tgtatacacg ctctggagag gtcaccatat
     3781 ccatcgagct gaagaagtct ggtttcacat tgtctcaaca aatgctcgag ctgattacaa
     3841 gattgcacca gtacatattc tcacatattc tccggcttga gaagcctgcc ctggagttta
     3901 aacctacagg cgcggagtca gcctactgtg ttctacctct taatgttgtt aatgactccg
     3961 gcactttgga cattgacttt aaattcatgg aggatattga gaagtcggaa gctcggatag
     4021 gcattcctag caccaagtat tcaaaggaaa caccgtttgt ctttaagtta gaagattacc
     4081 aagatgcagt gatcattcca agataccgca attttgatca gcctcatcga ttttatgtag
     4141 ctgatgtata tacggatctt accccactca gtaaatttcc ttcccctgaa tatgaaactt
     4201 ttgcagaata ttataaaaca aagtataacc ttgacctgac caatctcaac cagccactgc
     4261 tggatgttga ccacacatct tccagactga accttttgac acctcggcat ttgaatcaga
     4321 aggggaaagc acttcctcta agcagtgctg agaagaggaa ggccaaatgg gaaagtctgc
     4381 agaacaaaca gatcctggtt ccggaactct gtgctataca tccaatccca gcatcactgt
     4441 ggagaaaagc ggtttgtctc cccagcatac tgtatcgcct tcactgcctt ctgactgcag
     4501 aggaactaag agctcagaca gccagcgatg ctggtgtggg tgtcagatca cttcccgtgg
     4561 attttagata ccctaactta gacttcgggt ggaaaaaatc tattgatagc aagtctttca
     4621 tctcaagctg taactcctcg ttggctgaga gtgataatta ctgtaagcac agcacaactg
     4681 tagtccctga acatgctgca catcaaggtg ctactagacc ctccttagaa aaccatgacc
     4741 aaatgtctgt gaactgcaaa aggttgcccg ctgagtcacc tgctaagctc caaagtgagg
     4801 tttcaacgga tcttacagca attaatggtc tttcttacaa taaaaacctt gccaatggca
     4861 gttatgattt agttaacaga gacttttgcc aaggaaatca gctgaattac ttcaagcagg
     4921 aaatacctgt acaaccaact acctcatatc ccattcagaa tttgtacaat tatgagaacc
     4981 agcccaagcc cagcaatgaa tgtcctctac tgagtaatac gtaccttgat gggaacgcta
     5041 acacatctac ctcagatgga agccccgcgg tgtccacgat gcctgccatg atgaatgctg
     5101 ttaaagcact caaggacagg atggactctg agcagagccc ttctgttggg tactcctcaa
     5161 ggactcttgg ccccaatccg ggactgattc tccaggcttt gacactgtca aatgccagtg
     5221 atgggtttaa cctggagcgg cttgagatgc ttggcgactc ctttttaaag catgctatca
     5281 ccacatatct gttttgcacg taccctgatg ctcatgaagg ccgcctttca tatatgagaa
     5341 gcaaaaaggt cagcaactgt aacctgtatc gccttggcaa gaagaaggga ctgcccagcc
     5401 gcatggtggt gtcgatattc gatcctcctg tgaattggct tcctcctggt tatgtggtaa
     5461 accaagacaa aagtaactca gagaagtggg aaaaggatga aatgacaaaa gactgcttgc
     5521 tggctaatgg caaactgggt gaggcctgtg aggaggagga ggacctgacg tggagggccc
     5581 ccaaggagga ggccgaggat gaggacgact tcctggagta tgaccaggag catatccagt
     5641 ttatagacag catgctcatg ggctcgggcg ccttcgtgag gaaaatctct ctgtcgccct
     5701 tctcagcctc cgactctgca tacgaatgga agatgcccaa gaaggcctcc ttgggcagta
     5761 tgccgtttgc ctcgggcctt gaggattttg attacagctc ttgggatgcg atgtgctatc
     5821 tggatcctag caaagccgtt gaagaggatg actttgtggt aggtttctgg aatccatcag
     5881 aagagaactg tggcgtcgac acagggaagc agtccatttc ttacgacttg cacacggagc
     5941 agtgcattgc tgacaagagc atagcggact gtgtggaggc cctgctgggc tgctacttaa
     6001 ccagctgcgg cgagagggct gctcagctct tcctctgttc gctggggctg aaggtgctcc
     6061 cagtaatcaa aaggaccagc cgggagaagg ccttggaccc tgctcaggag aacggaagca
     6121 gccaacaaaa gagcctttca gggagctgtg cctctccagt tggcccacgc tcctctgcag
     6181 ggaaagactt ggagtacggc tgcctgaaga tcccaccgag gtgcatgttt gaccatccag
     6241 acgcagagaa aaccctcaac cacctgatat ctgggtttga aacttttgag aagaaaatta
     6301 actatagatt caagaataag gcttatcttc tgcaggcttt tacacacgcc tcctaccact
     6361 acaacactat cactgattgt taccagcgct tagaattcct gggagatgcg attttggact
     6421 acctcataac caagcacctt tatgaagacc cacggcagca ttctccaggg gtcttgactg
     6481 acttgcgctc tgcccttgtc aataacacca tctttgcatc attggctgtg aagtatgact
     6541 accacaagta ctttaaagct gtctctcctg agctctttca tgttattgat gactttgtga
     6601 agtttcagct tgagaagaac gaaatgcaag gaatggactc tgagcttagg agatccgagg
     6661 aggatgagga gaaagaagag gatatcgaag tccccaaggc catgggggac atttttgagt
     6721 ctcttgctgg tgccatttat atggacagtg ggatgtctct ggaggtggtc tggcaggtgt
     6781 actatccgat gatgcagcct ctaatagaaa agttttctgc aaatgtgcct cgttctccag
     6841 tgagagaatt gctcgagatg gaaccagaaa ctgccaagtt tagcccagcg gagagaactt
     6901 atgatggaaa ggtcagagtc accgtggaag tggtgggaaa ggggaaattt aaaggtgttg
     6961 gccggagcta caggatcgcc aagtctgctg cagcacgaag ggccctccgc agcctcaaag
     7021 ctaaccagcc tcaggttcct aacagctcgc tagagggccc gcggttcgaa caaaaactca
     7081 tctcagaaga ggatctgaat atgcataccg gtcatcatca ccatcaccat tgagtttaaa
     7141 cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
     7201 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
     7261 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
     7321 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta
     7381 tggcttctga ggcggaaaga acccggtagg cgccaaccgg ctccgttctt tggtggcccc
     7441 ttcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg
     7501 tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc
     7561 accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc
     7621 ttcgctttct gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg
     7681 ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa
     7741 aagcgcacgt ctgccgcgct gttctcctct tcctcatctc cgggcctttc gacctgcagc
     7801 ccaagctagc atcggcatag tatatcggca tagtataata cgacaaggtg aggaactaaa
     7861 ccatggccaa gcctttgtct caagaagaat ccaccctcat tgaaagagca acggctacaa
     7921 tcaacagcat ccccatctct gaagactaca gcgtcgccag cgcagctctc tctagcgacg
     7981 gccgcatctt cactggtgtc aatgtatatc attttactgg gggaccttgt gcagaactcg
     8041 tggtgctggg cactgctgct gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg
     8101 gaaatgagaa caggggcatc ttgagcccct gcggacggtg ccgacaggtg cttctcgatc
     8161 tgcatcctgg gatcaaagcc atagtgaagg acagtgatgg acagccgacg gcagttggga
     8221 ttcgtgaatt gctgccctct ggttatgtgt gggagggcta agcacttcgt ggccgaggag
     8281 caggactgac acgtgctacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     8341 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     8401 gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     8461 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     8521 aaactcatca atgtatctta tcatgtctgt atgcatggtg gtaatacggt tatccacaga
     8581 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     8641 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     8701 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     8761 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     8821 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     8881 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     8941 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     9001 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     9061 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     9121 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     9181 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     9241 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     9301 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     9361 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaac ctgaggctga
     9421 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     9481 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     9541 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     9601 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     9661 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     9721 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     9781 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     9841 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     9901 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     9961 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
    10021 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
    10081 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
    10141 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
    10201 cagcgtttca gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
    10261 gacacggaaa tgttgaatac tcatcctcag gactctccct ttttcaatat tattgaagca
    10321 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
    10381 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgcgccc tgtagcggcg
    10441 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
    10501 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
    10561 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
    10621 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
    10681 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
    10741 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
    10801 cggcctattg gttaaaaaat
//
