LOCUS       sequence-240228-         542 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used to sense miRNA activity using fluorescence based tools. 
            hsa-miR-210-5p target in 3' UTR of of mKate. miRNA activity can be 
            measured by the repression of mKate2 relative to EBFP2 fluorescent 
            proteins . Plasmid is inherently unstable; please see Depositor 
            Comments.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Gam JJ, Babb J, Weiss R
  TITLE     A mixed antagonistic/synergistic miRNA repression model enables 
            accurate predictions of multi-input miRNA sensor activity.
  JOURNAL   Nat Commun. 2018 Jun 22;9(1):2430. doi: 10.1038/s41467-018-04575-0.
  PUBMED    29934631
REFERENCE   2  (bases 1 to 542)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-06-22"; volume: "9(1):2430. doi"; pages: " 
            10.1038/s41467-018-04575-0"
COMMENT     Sequence Label: LSB-hsa-miR-210-5p
FEATURES             Location/Qualifiers
     source          1..542
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(96..120)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(344..363)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    409..464
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(463..482)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 caaagagaca tacgtcgagc agcacgaggt ggctgtggcc agatactgcg acctccctag
       61 caaactgggg cacaaactta attgagaatt ctaccaccca gctttcttgt acaaagtggt
      121 acgtctcaag accagaggtc tctgcttaca tcgtccagtg tgcggtgggc aggggctcag
      181 tgtgcggtgg gcaggggctc agtgtgcggt gggcaggggc tcagtgtgcg gtgggcaggg
      241 gctcaaccag aagagacctg tgcttcactc ctcaggtgca ggctgcctat cagaaggtgg
      301 tggctggtgt ggccaatgcc ctggctcaca aataccactg agatcttttt ccctctgcca
      361 aaaattatgg ggacatcatg aagccccttg agcatctgac ttctggctaa taaaggaaat
      421 ttattttcat tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg
      481 ggagggcaaa tcatttaaaa catcagaatg agtatttggt ttagagtttg gcaacatatg
      541 cc
//
