LOCUS       sequence-240229-        9687 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9687)
  AUTHORS   Guarguaglini G, Duncan PI, Stierhof YD, Holmstrom T, Duensing S, 
            Nigg EA
  TITLE     The forkhead-associated domain protein Cep170 interacts with 
            Polo-like kinase 1 and serves as a marker for mature centrioles.
  JOURNAL   Mol Biol Cell. 2005 Mar;16(3):1095-107. Epub 2004 Dec 22.
  PUBMED    15616186
REFERENCE   2  (bases 1 to 9687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2005-03"; volume: "16(3)"; pages: "1095-107. Epub 
            2004 Dec 22"
COMMENT     Sequence Label: pEGFP Cep170 (Nigg PID200)
FEATURES             Location/Qualifiers
     source          1..9687
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     613..632
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        613..631
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      659..668
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(665..667,668..670,671..1381)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(665..667,668..670,671..1381)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(710..731)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(971..990)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1318..1339
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6371..6423
                     /label=MCS
                     /note="multiple cloning site"
     polyA_signal    6547..6668
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6584..6603)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6638..6657
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(6675..7130)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6812..6833)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7024..7043
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        7157..7261
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7286..7306)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7330..7540
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        7352..7548
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7399..7534
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7583..8377
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7583..8377
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7637..7656)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8247..8266
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8565..8584)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8609..8656
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      8985..9573
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9474..9493
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg
      601 gcttatcgaa attaatacga ctcactatag ggagacccaa gctctagcgc taccggtcgc
      661 caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
      721 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
      781 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
      841 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
      901 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
      961 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     1021 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     1081 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     1141 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     1201 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     1261 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     1321 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     1381 gtccggactc agatctcgat cgacgagctt aacatcctgg tttttggtga gcagtggagg
     1441 cactcgccac aggctgccac gagaaatgat ttttgttgga agagatgact gtgagctcat
     1501 gttgcagtct cgtagtgtgg ataagcaaca cgctgtcatc aactatgatg cgtctacgga
     1561 tgagcattta gtgaaggatt tgggcagcct caatgggact tttgtgaatg atgtaaggat
     1621 tccggaacag acttatatca ccttgaaact tgaagataag ctgagatttg gatatgatac
     1681 aaatcttttc actgtagtac aaggagaaat gagggtccct gaagaagctc ttaagcatga
     1741 gaagtttacc attcagcttc agttgtccca aaaatcttca gaatcagaat tatccaaatc
     1801 tgcaagtgcc aaaagcatag attcaaaggt agcagacgct gctactgaag tgcagcacaa
     1861 aactactgaa gcactgaaat ccgaggaaaa agccatggat atttctgcta tgccccgtgg
     1921 tactccatta tatgggcagc cgtcatggtg gggggatgat gaggtggatg aaaaaagagc
     1981 tttcaagaca aatggcaaac ctgaagaaaa aaaccatgaa gctggaacat cagggtgcag
     2041 catagatgcc aagcaagttg aggaacaatc tgcagctgca aatgaagaag tactttttcc
     2101 tttctgtagg gaaccaagtt attttgaaat ccctacaaaa gaattccagc aaccatcaca
     2161 aataacagaa agcactattc atgaaatccc aacaaaagac acgccaagtt cccatataac
     2221 aggtgcaggg catgcttcat ttaccattga atttgatgac agtaccccag ggaaggtaac
     2281 tattagagac catgtgacaa agtttacttc tgatcagcgc cacaagtcca agaagtcttc
     2341 tcctggaact caagacttgc tggggattca aacaggaatg atggcacccg aaaacaaagt
     2401 tgctgactgg ctagcacaaa acaaccctcc tcaaatgcta tgggaaagaa cagaagagga
     2461 ttctaaaagc attaaaagtg atgttccagt gtacttgaaa aggttgaaag gaaataaaca
     2521 tgatgatggt acgcaaagtg attcagagaa cgctggggct cacaggcgct gtagcaaacg
     2581 tgcaactctt gaggaacact taagacgcca ccattcagaa cacaaaaagc tacagaaggt
     2641 ccaggctact gaaaagcatc aagaccaagc tgttgtgttt ggagtagatg acaatcagga
     2701 ttataatagg cctgttatca acgaaaaaca taaagatcta ataaaagatt gggctctcag
     2761 ttctgctgca gcagtaatgg aagaaagaaa accactgact acatctggat ttcaccactc
     2821 agaggaaggc acatcttcat ctggaagcaa acgttgggtt tcacagtggg ctagtttggc
     2881 tgccaatcat acaaggcatg atcaagaaga aaggataatg gaattttctg cacctcttcc
     2941 tttagagaat gagacagaga tcagtgagtc tggcatgaca gtgagaagta ctggctctgc
     3001 aacttccttg gctagccagg gagagagaag gagacgaact cttccccagc ttccaaatga
     3061 agaaaagtct cttgagagcc acagagcaaa ggttgtaaca cagaggtcag agataggaga
     3121 aaaacaagac acagaacttc aggagaaaga aacacctaca caggtatacc agaaagataa
     3181 acaagatgct gacagaccct tgagtaaaat gaacagggca gtaaatggag agactctcaa
     3241 aactggtgga gataataaaa ccctacttca cttaggcagc tctgctcctg gaaaagagaa
     3301 aagtgaaact gataaggaaa cttctttggt aaagcaaaca ttagcaaaac ttcaacaaca
     3361 agaacaaagg gaggaggctc agtggacacc tactaaattg tcttccaaaa atgtttcagg
     3421 tcagacagat aaatgtaggg aggaaacttt taaacaagaa tcacaacctc cagaaaaaaa
     3481 ttcaggacat tctacaagca aaggagacag agtggcacaa agtgagagca agagaagaaa
     3541 agctgaggaa attctgaaaa gtcagactcc aaagggagga gacaagaagg aatcctccaa
     3601 gtcattagtg cgacaaggga gcttcactat agaaaaaccc agcccaaaca tacccataga
     3661 acttattccc catataaata aacagacttc ctctactcct tcttctttag cattaacatc
     3721 tgcaagtaga atacgagaaa gaagtgagtc tttggatcct gattctagta tggacacaac
     3781 ccttattcta aaagacacag aagcagtaat ggcttttcta gaagctaaac tacgtgaaga
     3841 taataaaact gatgaaggac cagatactcc cagttataat agagacaatt ctatttcacc
     3901 agaatctgat gtagatacag ctagtacaat cagtctggtt actggagaaa ctgaaagaaa
     3961 gtcaacccaa aagcgaaaga gtttcactag cctctataaa gataggtgtt ccacaggttc
     4021 tccttccaaa gatgttacaa aatcatcatc ttcaggtgct agggaaaaaa tggaaaagaa
     4081 aacaaaaagt cgttccacag atgtgggttc aagagcagat ggtcgtaaat ttgttcagtc
     4141 cagtgggaga ataagacagc cctcagtaga cttaacagat gatgaccaaa cctctagtgt
     4201 acctcattct gccatctctg atattatgtc atctgatcaa gaaacttact cttgtaaacc
     4261 tcatggacgg actccactta cctcagctga tgagcatgta cattccaaac tggaaggaag
     4321 taaagtaacg aaatctaaga cttctccggt ggtatctggt tcatctagta aatcaaccac
     4381 ccttccaagg ccacgaccta ccaggacttc cctcttgcgc agagcacgac ttggtgaagc
     4441 ttcagacagt gaacttgctg atgctgacaa agcatctgtt gcttctgaag tatccacaac
     4501 aagttctaca tcaaaacctc ccacaggaag gcgtaacatc tctcggattg atttattggc
     4561 tcagcctcgt agaacacgac ttggctcact gtcagctcgt agtgactctg aagcaacaat
     4621 ttctagaagt agtgcctctt cgaggaccgc agaagccatc attagaagtg gagccagact
     4681 agtaccatca gataaatttt ctcctagaat tagagctaac agtatctctc gactctcaga
     4741 ctccaaggtc aaaagtatga cctcagctca tggctctgct tcagccctga aaaccactcg
     4801 cttgcagagc gctggatcag caatgcctac tagttcttca ttcaaacacc ggattaaaga
     4861 gcaggaagac tacatccgag attggactgc tcatcgagaa gagatagcca ggatcagcca
     4921 agatcttgct ctcattgctc gggagatcaa cgatgtagca ggagagatag attcagtgac
     4981 ttcatcaggc actgccccta gtaccacagt aagcactgct gccaccaccc ctggctctgc
     5041 catagacact agagaagagg taggagatct tcatggagaa atgcataagt tggttgatcg
     5101 tgtttttgat gaaagcctca acttccgaaa gattcctcca ttagttcatt ccaaaacacc
     5161 agaaggaaac aacggtcgat ctggtgatcc aagacctcaa gcagcagagc ctcccgatca
     5221 cttaacaatt acaaggcgga gaacctggag cagggatgaa gtcatgggag ataatctgct
     5281 gctgtcatcc gtctttcagt tctctaagaa gataagacaa tctatagata agacagctgg
     5341 aaagatcaga atattattta aagacaaaga tcggaattgg gatgacatag aaagcaaatt
     5401 aagagccgaa agtgaagtcc ctattgtgaa aacctcaagc atggagattt cttctatctt
     5461 acaggaactg aaaagagtag aaaagcagct gcaagcaatc aatgctatga ttgatcctga
     5521 tggaactttg gaggctctga acaacatggg atttcccagt gctatgttgc catctccacc
     5581 gaaacagaag tccagccctg tgaataacca ccacagcccg ggtcagacac caacacttgg
     5641 ccaaccagaa gctagggctc ttcatcctgc tgctgtttca gccgcagctg aatttgagaa
     5701 tgctgaatct gaggctgatt tcagtataca tttcaataga ttcaaccccg atggggaaga
     5761 ggaagatgtt acagtacaag aatgactttc tcttgattgt tgaaaaatca ttacctgtgg
     5821 aatggctagg aatattggaa gcagcatagt gttgatgtac gcaaaacaag acagcttggt
     5881 cagctacaat cttggaatcc ctgtcttctt aattttattt atttattttt gacgtataat
     5941 gtagtatatc aatcctttca aactatttag ataaccactt gatgcacaaa taggaaaaag
     6001 cagattgtgg cagtgtcgcc ttttgtggtt ttatgatttt caaattgaat ttaatgatta
     6061 caccctttcc cttcatagat cttttttctt ttttttaagc catgctgtga cctacaagca
     6121 aactaaatag ccaacatttc tgaaccccta agtctcctgt gccaagctgc tccctgaaat
     6181 ggacttcttc atctgtacag atttgttaaa ccattctatt tgcttcttaa taataggatt
     6241 tatattagta ctcattacca ttggacacaa tgacataagt actctccaca gtaaagcaga
     6301 cctttcacaa cagtcactct gtgtcctaaa attttccaac atagatgtga tttatataac
     6361 tttgttgata ctcgagctca agcttcgaat tctgcagtcg acggtaccgc gggcccggga
     6421 tccaccggat ctagataact gatcataatc agccatacca catttgtaga ggttttactt
     6481 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
     6541 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     6601 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     6661 gtatcttaac gcgtaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg
     6721 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
     6781 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
     6841 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
     6901 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
     6961 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
     7021 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
     7081 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac
     7141 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     7201 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     7261 tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     7321 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     7381 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     7441 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     7501 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaga tcgatcaaga
     7561 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc
     7621 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
     7681 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
     7741 gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac
     7801 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
     7861 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
     7921 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
     7981 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
     8041 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
     8101 gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt
     8161 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
     8221 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
     8281 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
     8341 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg
     8401 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat
     8461 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
     8521 gatctcatgc tggagttctt cgcccaccct agggggaggc taactgaaac acggaaggag
     8581 acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggt
     8641 gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc
     8701 ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc
     8761 ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata
     8821 gcctcaggtt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     8881 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     8941 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     9001 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     9061 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     9121 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     9181 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     9241 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     9301 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     9361 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     9421 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     9481 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     9541 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     9601 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     9661 gtggataacc gtattaccgc catgcat
//
