LOCUS       sequence-240236-        3436 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  shRNA targeting the coding region of the gephyrin mRNA .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3436)
  AUTHORS   Yu W, Jiang M, Miralles CP, Li RW, Chen G, de Blas AL
  TITLE     Gephyrin clustering is required for the stability of GABAergic 
            synapses.
  JOURNAL   Mol Cell Neurosci. 2007 Dec;36(4):484-500. doi: 
            10.1016/j.mcn.2007.08.008. Epub 2007 Aug 23.
  PUBMED    17916433
REFERENCE   2  (bases 1 to 3436)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.mcn.2007.08.008"; journalName: "Mol Cell Neurosci"; date:
            "2007-12"; volume: "36"; issue: "4"; pages: "484-500"
COMMENT     Sequence Label: Geph CR shRNA
FEATURES             Location/Qualifiers
     source          1..3436
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        100..412
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     331..351
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     polyA_signal    487..621
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(511..530)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     565..584
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(719..739)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(719..737)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(758..774)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(758..774)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(771..793)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    782..798
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(806..812,813..830,831..836))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    851..872
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(989..1006)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1160..1748)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1240..1259)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1919..2710,2711..2779))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2542..2561
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2780..2884)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2910..3365)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3047..3068)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3259..3278
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tcgacagcct gcaggaatgg ccggccatct taattaagaa atttaaatat gtttaaacat
       61 agggcccaat ggcgcgccaa ggccatctag gccaagctta tccgacgccg ccatctctag
      121 gcccgcgccg gccccctcgc acagacttgt gggagaagct cggctactcc cctgccccgg
      181 ttaatttgca tataatattt cctagtaact atagaggctt aatgtgcgat aaaagacaga
      241 taatctgttc tttttaatac tagctacatt ttacatgata ggcttggatt tctataagag
      301 atacaaatac taaattatta ttttaaaaaa cagcacaaaa ggaaactcac cctaactgta
      361 aagtaattgt gtgttttgag actataaata tcccttggag aaaagccttg tttggaggta
      421 gagctggcag tatgaactga cacagttcat acagccagct ctacctcttt ttctagataa
      481 tacgtagatc cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
      541 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
      601 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag
      661 ggggaggtgt gggaggtttt ttaattcttg cggccgcggc ggtacccagc ttttgttccc
      721 tttagtgagg gttaattgcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa
      781 attgttatcc gctcacaatt ccacacaaca tacgagccgg gagcataaag tgtaaagcct
      841 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
      901 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
      961 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     1021 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     1081 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     1141 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     1201 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     1261 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     1321 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     1381 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     1441 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     1501 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     1561 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     1621 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     1681 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     1741 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     1801 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     1861 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     1921 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     1981 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     2041 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     2101 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     2161 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     2221 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     2281 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     2341 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     2401 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     2461 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     2521 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     2581 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     2641 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     2701 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     2761 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     2821 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     2881 cgcgcacatt tccccgaaaa gtgccaccta aattgtaagc gttaatattt tgttaaaatt
     2941 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat
     3001 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa
     3061 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
     3121 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa
     3181 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
     3241 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag
     3301 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg
     3361 cgcgtcccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
     3421 ttcgctatta cgccag
//
