LOCUS       sequence-240240-       10443 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cre/Lox C-terminal tagging construct encoding 13 copies of the human
            c-myc (Myc) epitope.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10443)
  AUTHORS   Watson AT1, Garcia V, Bone N, Carr AM, Armstrong J.
  TITLE     Gene tagging and gene replacement using recombinase-mediated 
            cassette exchange in Schizosaccharomyces pombe.
  JOURNAL   Gene. 2008 Jan 15;407(1-2):63-74
  PUBMED    18054176
REFERENCE   2  (bases 1 to 10443)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene."; 
            date: "2008-01-15"; volume: "407(1-2)"; pages: "63-7"
COMMENT     Sequence Label: pAW8_13Myc
FEATURES             Location/Qualifiers
     source          1..10443
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      575..1356
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     primer_bind     complement(1451..1470)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1570..1592
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1630..1648)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1716..1820
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1821..1889,1890..2681)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2039..2058)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2852..3440
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3341..3360
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3594..3611
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3728..3749
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3764..3769,3770..3787,3788..3794)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3802..3818
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3807..3829
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3826..3842
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3826..3842
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     gene            complement(3953..5309)
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     terminator      complement(3953..4150)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(4156..4965)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     4879..4898
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(4966..5309)
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     terminator      complement(5384..5571)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     5492..5516
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(5626..6123)
                     /codon_start=1
                     /product="13 tandem Myc epitope tags"
                     /label=13xMyc
                     /translation="EQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLIS
                     EEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISEEDLNGEQKLISEEDLNGLD
                     GEQKLISEEDLNGEQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISE
                     EDL"
     protein_bind    6132..6165
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(6181..6198)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6181..6197)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6190..6212)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             9092..10186
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
ORIGIN
        1 caaaaagtta ggtgtaacag aaaatcatga aactatgatt tctaatttat atattggagg
       61 attttctcta aaaaaaaaaa tacaacaaat aaaaaacact caatgacctg accatttgat
      121 ggagtttaag tcaatacctt cttgaaccat ttcccataat ggtgaaagtt ccctcaagaa
      181 ttttactctg tcagaaacgg ccttaacgac gtagtcgatc cggacgatgg ccaaccttcc
      241 aattcattaa atcgagtcct ccatcagatg atttacaagg ttttgcatag aatccctaag
      301 taagagaagt tgttagtgat ttcttcaagt ttactaaaat ttacatacaa atggatgatc
      361 tcttctccat tgtttccttt cttcttgcaa acgcgtttta caaagagatg acatgctatt
      421 ttctgattat ttttttctat agttttctat tttgtacgtc cttgggagtc cgaaatgtaa
      481 aatcggggta tggttagtag cggcagcgtt cattatggta tataaaagat aaaaagtagg
      541 ggcaagcgag acagaatggg atacaagggc atcgtctata attatagcta aaaattgtat
      601 tttaatttgt attttttgta attttatttc tcattgtttt acttaaaatg aatgcgaatt
      661 agagaaaact tcaacgaaat gtcaaaataa gctcagcaaa atatacaatt ttagggaaag
      721 cgatcagcaa cttattctcc gttgtagatt ctttttcttc ataaattcca acaatgtaac
      781 tgccaattga tttttctcta gatgctaata aattgaaact aatgaatata ataacacagg
      841 ttttgataaa ttgtaaactc ttactcaaaa aaagaatgtt aaaactaatt taaccttttt
      901 ataaaggctc atggcgttaa agaggatagt aaaaattcta caatttttta atttaatgtc
      961 acaattgtct tgcgaatata attctattaa ttttcgctaa ctgctgtttt agtactatta
     1021 ctttcaatat gccttatatg caactttaat tcatgaagaa tgcaatattt ttgcaatctt
     1081 tcttgtactg agcgcttttt atcatttagt actcattatt taatttttca gtaaagggca
     1141 ataaataaat ttgtagaaga tgcaatgtaa tctcctctat ccttttgctc atatgtttat
     1201 gagtatacct agtctagaaa ggcttgtatt taaaatatta ttcaataaaa attcacaatt
     1261 tttacgacat gtgctaccat tcacttaact tcctgattat aaaattggtt cgtttatact
     1321 aattacttaa gtacctttaa ctaaacaaaa tgcctatata tatattaatt tacaatgagt
     1381 gtcagataag tcactatgtc cgagtggtta aggagttaga ctcgccatcg atggtatggt
     1441 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
     1501 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     1561 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     1621 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     1681 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     1741 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     1801 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     1861 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     1921 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     1981 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     2041 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     2101 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     2161 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     2221 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     2281 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     2341 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     2401 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     2461 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     2521 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     2581 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     2641 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     2701 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     2761 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     2821 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     2881 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     2941 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     3001 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     3061 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     3121 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     3181 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     3241 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     3301 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     3361 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     3421 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     3481 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     3541 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     3601 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     3661 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     3721 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     3781 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     3841 accatgatta cgccaagctt gcatgcgcgg ccgcataact tcgtatataa taccatatac
     3901 gaagttatac tagttcgtcg actggatggc ggcgttagta tcgaatcgac agcagtatag
     3961 cgaccagcat tcacatacga ttgacgcatg atattacttt ctgcgcactt aacttcgcat
     4021 ctgggcagat gatgtcgagg cgaaaaaaaa tataaatcac gctaacattt gattaaaata
     4081 gaacaactac aatataaaaa aactatacaa atgacaagtt cttgaaaaca agaatctttt
     4141 tattgtcagt actgattaga aaaactcatc gagcatcaaa tgaaactgca atttattcat
     4201 atcaggatta tcaataccat atttttgaaa aagccgtttc tgtaatgaag gagaaaactc
     4261 accgaggcag ttccatagga tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc
     4321 aacatcaata caacctatta atttcccctc gtcaaaaata aggttatcaa gtgagaaatc
     4381 accatgagtg acgactgaat ccggtgagaa tggcaaaagc ttatgcattt ctttccagac
     4441 ttgttcaaca ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt
     4501 attcattcgt gattgcgcct gagcgagacg aaatacgcga tcgctgttaa aaggacaatt
     4561 acaaacagga atcgaatgca accggcgcag gaacactgcc agcgcatcaa caatattttc
     4621 acctgaatca ggatattctt ctaatacctg gaatgctgtt ttgccgggga tcgcagtggt
     4681 gagtaaccat gcatcatcag gagtacggat aaaatgcttg atggtcggaa gaggcataaa
     4741 ttccgtcagc cagtttagtc tgaccatctc atctgtaaca tcattggcaa cgctaccttt
     4801 gccatgtttc agaaacaact ctggcgcatc gggcttccca tacaatcgat agattgtcgc
     4861 acctgattgc ccgacattat cgcgagccca tttataccca tataaatcag catccatgtt
     4921 ggaatttaat cgcggcctcg aaacgtgagt cttttcctta cccatggttg tttatgttcg
     4981 gatgtgatgt gagaactgta tcctagcaag attttaaaag gaagtatatg aaagaagaac
     5041 ctcagtggca aatcctaacc ttttatattt ctctacaggg gcgcggcgtg gggacaattc
     5101 aacgcgtctg tgaggggagc gtttccctgc tcgcaggtct gcagcgagga gccgtaattt
     5161 ttgcttcgcg ccgtgcggcc atcaaaatgt atggatgcaa atgattatac atggggatgt
     5221 atgggctaaa tgtacgggcg acagtcacat catgcccctg agctgcgcac gtcaagactg
     5281 tcaaggaggg tattctgggc ctccatgtcg ctggccgggt gacccggcgg ggacgaggca
     5341 agctaaacag atctatatta ccctgttatc cctagcggat ctgccggtag aggtgtggtc
     5401 aataagagcg acctcatact atacctgaga aagcaacctg acctacagga aagagttact
     5461 caagaataag aattttcgtt ttaaaaccta agagtcactt taaaatttgt atacacttat
     5521 tttttttata acttatttaa taataaaaat cataaatcat aagaaattcg cttatttaga
     5581 agtggcgcga attcactagt gattgattaa tttttgttca ccgttcaagt cttcctcgga
     5641 gattagcttt tgttcaccgt tcaaatcttc ttcagaaatc aacttttgtt caccgtcgag
     5701 tccgttcaag tcttcttctg agattaattt ttgttcaccg ttcaagtctt cctcggagat
     5761 tagcttttgt tcaccgttca aatcttcttc agaaatcaac ttttgttcac cgtcgagtcc
     5821 gttcaagtct tcttctgaga ttaatttttg ttcaccgttc aagtcttcct cggagattag
     5881 cttttgttca ccgttcaaat cttcttcaga aatcaacttt tgttcaccgt cgagtccgtt
     5941 caagtcttct tctgagatta atttttgttc accgttcaag tcttcctcgg agattagctt
     6001 ttgttcaccg ttcaaatctt cttcagaaat caacttttgt tcaccgtcga gtccgttcaa
     6061 gtcttcttct gagattaatt tttgttcacc gttcaagtct tcctcggaga ttagcttttg
     6121 ttcaagcatg cataacttcg tataatgtat gctatacgaa gttatgcggc cgctgaattc
     6181 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     6241 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
     6301 cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt attttctcct
     6361 tacgcatctg tgcggtattt cacaccgcat accatcgtcc ggatcaatga agtagatgag
     6421 tttactacct gtatatactt ttttttttgt caaagtcact tttatggcat ttccatgttg
     6481 aaggattagc cacagcacta aattttcagc gtatgattgc ttttaaatat ttaattttca
     6541 tcgtttttta attaatattc aaaacgattt aatgccttct acatctgaag tcaagctcat
     6601 agactcgggt tgggtagcta aaactacatc taacacgaag gggtatagcc ttcttaaagt
     6661 atactcatgt ataaacgttt tgggttcata tttttgggcc acgtggttta taaaaattct
     6721 taactacacc actcggtatc ccgcacccgt ctacgtttct acgatttcga gctaatattg
     6781 agtaaagtga ttagcaaaaa aaataaaagt actcgttgtc ggagatcaag aatttttcta
     6841 ttatctcatc taaaccactt tctaaaagcg aaaaacaaaa tcgtaatatg cagcttgaat
     6901 gggcttccat agtttgaaag aaaaacccta gcagtactgg caagggagac attcctttta
     6961 cctggctaat cgccatcttc cagcaggcgc accattgccc ctgtttcact atccaggtta
     7021 cggatatagt tcatgacaat atttacattg gtccagccac cagcttgcat gatctccggt
     7081 attgaaactc cagcgcgggc catatctcgc gcggctccga cacgggcact gtgtccagac
     7141 caggccaggt atctctgacc agagtcatcc ttagcgccgt aaatcaatcg atgagttgct
     7201 tcaaaaatcc cttccagggc gcgagttgat agctggctgg tggcagatgg cgcggcaaca
     7261 ccattttttc tgacccggca aaacaggtag ttattcggat catcagctac accagagacg
     7321 gaaatccatc gctcgaccag tttagttacc cccaggctaa gtgccttctc tacacctgcg
     7381 gtgctaacca gcgttttcgt tctgccaata tggattaaca ttctcccacc gtcagtacgt
     7441 gagatatctt taaccctgat cctggcaatt tcggctatac gtaacagggt gttataagca
     7501 atccccagaa atgccagatt acgtatatcc tggcagcgat cgctattttc catgagtgaa
     7561 cgaacctggt cgaaatcagt gcgttcgaac gctagagcct gttttgcacg ttcaccggca
     7621 tcaacgtttt cttttcggat ccgccgcata accagtgaaa cagcattgct gtcacttggt
     7681 cgtggcagcc cggaccgacg atgaagcatg tttagctggc ccaaatgttg ctggatagtt
     7741 tttactgcca gaccgcgcgc ctgaagatat agaagataat cgcgaacatc ttcaggttct
     7801 gcgggaaacc atttccggtt attcaacttg caccatgccg cccacgaccg gcaaacggac
     7861 agaagcattt tccaggtatg ctcagaaaac gcctggcgat ccctgaacat gtccatcagg
     7921 ttcttgcgaa cctcatcact cgttgcatcg accggtaact tttagattaa catttgttta
     7981 aatatgaaat taggcaatgt aacatactgc aggcaaattt tggtgtacgg taagtaaatt
     8041 ggacatatga tttaacaaag cgactataag tcagaaagtg agaatgagat tgaaataatt
     8101 aattcaactt attcaattga tgatatgcca ggattcctct tcctttatct tcaatgtttc
     8161 gattaacagg tccattatcc ggtttttagt ttcgctgcac attgccgaat gacaatcgga
     8221 ttcctttaag ctttgctttt gccctgtttt ctctccccag taatcgttgg gtgactgaac
     8281 catccagtag ccgagagtta cgtttttcct gatttaataa atatccatat ctatttgtct
     8341 cgtggtgttt tacagcaact aaccgaaatt actattttgc atcatccaac cataaagaga
     8401 caacaataat tctagaaaac atataacaag tgtttttttc tatttacgtc tcatacgcaa
     8461 agtccacttt accatgtttt ggaaacttgt acccaaccat tcatttttcg attttgtaat
     8521 gaggctcttt tcttctaaca atcattgagg tcattatcat gtgtcctcac ctattgttta
     8581 aattgaggct tgcgctattc ccaaaattgt gggtattatt ttcatatatc agtatctgca
     8641 atttcggttc aactccggaa gctcggtacc caactgtggg aatactcagg tatcgtaaga
     8701 tgcaagagtt cgaatctctt agcaaccatt attttttttc tctcaacata acgagaacac
     8761 acaggggcgc tatcgcacag aatcaaattc gatgactgga aattttttgt taatttcaga
     8821 ggtcgcctga cgcatatacc tttttcaact gaaaaattgg gagaaaaagg aaaggtgaga
     8881 gcgccggaac cggcttttca tatagaatag agaagcgttc atgactaaat gcttgcatca
     8941 caatacttga agttgacaat attatttaag gacctattgt tttttccaat aggtggttag
     9001 caatcgtctt actttctaac ttttcttacc ttttacattt cagcaatata tatatatata
     9061 tatatatata tttcaaggat ataccattgt aatgtctgcc cctaagaaga tcgtcgtttt
     9121 gccaggtgac cacgttggtc aagaaatcac agccgaagcc attaaggttc ttaaagctat
     9181 ttctgatgtt cgttccaatg tcaagttcga tttcgaaaat catttaattg gtggtgctgc
     9241 tatcgatgct acaggtgttc cacttccaga tgaggcgctg gaagcctcca agaaggctga
     9301 tgccgttttg ttaggtgctg tgggtggtcc taaatggggt accggtagtg ttagacctga
     9361 acaaggttta ctaaaaatcc gtaaagaact tcaattgtac gccaacttaa gaccatgtaa
     9421 ctttgcatcc gactctcttt tagacttatc tccaatcaag ccacaatttg ctaaaggtac
     9481 tgacttcgtt gttgttagag aattagtggg aggtatttac tttggtaaga gaaaggaaga
     9541 cgatggtgat ggtgtcgctt gggatagtga acaatacacc gttccagaag tgcaaagaat
     9601 cacaagaatg gccgctttca tggccctaca acatgagcca ccattgccta tttggtcctt
     9661 ggataaagct aatgttttgg cctcttcaag attatggaga aaaactgtgg aggaaaccat
     9721 caagaacgaa tttcctacat tgaaagttca acatcaattg attgattctg ccgccatgat
     9781 cctagttaag aacccaaccc acctaaatgg tattataatc accagcaaca tgtttggtga
     9841 tatcatctcc gatgaagcct ccgttatccc aggctccttg ggtttgttgc catctgcgtc
     9901 cttggcctct ttgccagaca agaacaccgc atttggtttg tacgaaccat gccatggttc
     9961 cgctccagat ttgccaaaga ataaggtcaa ccctatcgcc actatcttgt ctgctgcaat
    10021 gatgttgaaa ttgtcattga acttgcctga agaaggtaaa gccattgaag atgcagttaa
    10081 aaaggttttg gatgcaggta tcagaactgg tgatttaggt ggttccaaca gtaccaccga
    10141 agtcggtgat gctgtcgccg aagaagttaa gaaaatcctt gcttaaaaag attctctttt
    10201 tttgtgatat ttgtacataa actttataaa tgaaattcat aatagaaacg acacgaaatt
    10261 acaaaatgga atatgttcat agggtagacg aaactatata cgcaatctac atacatttat
    10321 caagaaggag aaaaaggagg atgtaaagga atacaggtaa gcaaattgat actaatggct
    10381 caacgtgata aggaaaaaga attgcacttt aacattaata ttgacaagga ggagggcatc
    10441 aca
//
