LOCUS       sequence-240241-         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used to sense miRNA activity using fluorescence based tools. 
            hsa-miR-30c-1-3p target in 3' UTR of of mKate. miRNA activity can be
            measured by the repression of mKate2 relative to EBFP2 fluorescent 
            proteins . Plasmid is inherently unstable; please see Depositor 
            Comments.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Gam JJ, Babb J, Weiss R
  TITLE     A mixed antagonistic/synergistic miRNA repression model enables 
            accurate predictions of multi-input miRNA sensor activity.
  JOURNAL   Nat Commun. 2018 Jun 22;9(1):2430. doi: 10.1038/s41467-018-04575-0.
  PUBMED    29934631
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-06-22"; volume: "9(1):2430. doi"; pages: " 
            10.1038/s41467-018-04575-0"
COMMENT     Sequence Label: LSB-hsa-miR-30c-1-3p
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(63..87)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(311..330)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    376..431
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(430..449)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 tgtggccaga tactgcgacc tccctagcaa actggggcac aaacttaatt gagaattcta
       61 ccacccagct ttcttgtaca aagtggtacg tctcaagacc agaggtctct gctttacttt
      121 ccggagtaaa caaccctctc ccagggagta aacaaccctc tcccagggag taaacaaccc
      181 tctcccaggg agtaaacaac cctctcccag caaccagaag agacctgtgc ttcactcctc
      241 aggtgcaggc tgcctatcag aaggtggtgg ctggtgtggc caatgccctg gctcacaaat
      301 accactgaga tctttttccc tctgccaaaa attatgggga catcatgaag ccccttgagc
      361 atctgacttc tggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt
      421 tgtgtctctc actcggaagg acatatggga gggcaaatca tttaaaacat cagaatgagt
      481 atttggttta gagtttggca acatatgccc atatgctggc tgccatgaac aaaggttggc
      541 tataaagagg tcatcagtat atgaaacagc cccctgctgt ccattcctta ttccatagaa
      601 aagccttgac ttgaggttag atttttttta tattttgttt tgtgttattt ttttctttaa
      661 catccctaaa attttcctta catgttttac tagccagatt tttcctcctc tcctgactac
      721 tcccagtcat agctgtccct cttctcttat ggagatcccg agcggtgcgt ttttatgctt
      781 gtagtattgt ataatgtttt taagatccaa gcttctgacc tcttctcttc ctcccac
//
