LOCUS       sequence-240245-       10634 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level2 MoClo construct, containing level1 plant expression cassettes
            for eSaCas9(pEPOR1CB0113), sgRNA{AT1G73460}(pEPOR1CB0099) and 
            sgRNA{AT1G73460}(pEPOR1CB0110) to Protein kinase superfamily 
            protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10634)
  AUTHORS   Raitskin O, Schudoma C, West A, Patron NJ
  TITLE     Comparison of efficiency and specificity of CRISPR-associated (Cas) 
            nucleases in plants: An expanded toolkit for precision genome 
            engineering.
  JOURNAL   PLoS One. 2019 Feb 27;14(2):e0211598. doi: 
            10.1371/journal.pone.0211598. eCollection 2019.
  PUBMED    30811422
REFERENCE   2  (bases 1 to 10634)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2019-02-27"; volume: "14(2):e0211598. doi"; pages: " 
            10.1371/journal.pone.0211598. eCollection 2019"
COMMENT     Sequence Label: pEPOR2KN0078
FEATURES             Location/Qualifiers
     source          1..10634
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        454..799
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     742..762
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             896..916
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4097..4144
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4151..4231
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             join(4238..4240,4241..4243,4244..4957)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4283..4304)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4544..4563)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4891..4912
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      4970..5222
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_RNA        5476..5551
                     /label=Sa gRNA scaffold
                     /note="guide RNA scaffold for the Staphylococcus aureus 
                     CRISPR/Cas9 system"
     misc_RNA        5818..5893
                     /label=Sa gRNA scaffold
                     /note="guide RNA scaffold for the Staphylococcus aureus 
                     CRISPR/Cas9 system"
     misc_feature    5972..5996
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6305..6934
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7363..8436
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      8502..8696
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     promoter        8758..8862
                     /gene="bla"
                     /label=AmpR promoter
     CDS             8863..9657
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      9828..10416
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10317..10336
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    10552..10576
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gaacactctg tgccgatgta tcacctctcg caagaattca agcttggagg tcaacatggt
       61 ggagcacgac actctggtct actccaaaaa tgtcaaagat acagtctcag aagatcaaag
      121 ggctattgag acttttcaac aaaggataat ttcgggaaac ctcctcggat tccattgccc
      181 agctatctgt cacttcatcg aaaggacagt agaaaaggaa ggtggctcct acaaatgcca
      241 tcattgcgat aaaggaaagg ctatcattca agatctctct gccgacagtg gtcccaaaga
      301 tggaccccca cccacgagga gcatcgtgga aaaagaagag gttccaacca cgtctacaaa
      361 gcaagtggat tgatgtgata acatggtgga gcacgacact ctggtctact ccaaaaatgt
      421 caaagataca gtctcagaag atcaaagggc tattgagact tttcaacaaa ggataatttc
      481 gggaaacctc ctcggattcc attgcccagc tatctgtcac ttcatcgaaa ggacagtaga
      541 aaaggaaggt ggctcctaca aatgccatca ttgcgataaa ggaaaggcta tcattcaaga
      601 tctctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa
      661 agaagaggtt ccaaccacgt ctacaaagca agtggattga tgtgacatct ccactgacgt
      721 aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat aaggaagttc
      781 atttcatttg gagaggacac gctcgagtat aagagctcat ttttacaaca attaccaaca
      841 acaacaaaca acaaacaaca ttacaattac atttacaatt atcgatacaa tggccccaaa
      901 gaagaagcgg aaggtcggta tccacggagt cccagcagcc aagcggaact acatcctggg
      961 cctggacatc ggcatcacca gcgtgggcta cggcatcatc gactacgaga cacgggacgt
     1021 gatcgatgcc ggcgtgcggc tgttcaaaga ggccaacgtg gaaaacaacg agggcaggcg
     1081 gagcaagaga ggcgccagaa ggctgaagcg gcggaggcgg catagaatcc agagagtgaa
     1141 gaagctgctg ttcgactaca acctgctgac cgaccacagc gagctgagcg gcatcaaccc
     1201 ctacgaggcc agagtgaagg gcctgagcca gaagctgagc gaggaagagt tctctgccgc
     1261 cctgctgcac ctggccaaga gaagaggcgt gcacaacgtg aacgaggtgg aagaggacac
     1321 cggcaacgag ctgtccacca aagagcagat cagccggaac agcaaggccc tggaagagaa
     1381 atacgtggcc gaactgcagc tggaacggct gaagaaagac ggcgaagtgc ggggcagcat
     1441 caacagattc aagaccagcg actacgtgaa agaagccaaa cagctgctga aggtgcagaa
     1501 ggcctaccac cagctggacc agagcttcat cgacacctac atcgacctgc tggaaacccg
     1561 gcggacctac tatgagggac ctggcgaggg cagccccttc ggctggaagg acatcaaaga
     1621 atggtacgag atgctgatgg gccactgcac ctacttcccc gaggaactgc ggagcgtgaa
     1681 gtacgcctac aacgccgacc tgtacaacgc cctgaacgac ctgaacaatc tcgtgatcac
     1741 cagggacgag aacgagaagc tggaatatta cgagaagttc cagatcatcg agaacgtgtt
     1801 caagcagaag aagaagccca ccctgaagca gatcgccaaa gaaatcctcg tgaacgaaga
     1861 ggatattaag ggctacagag tgaccagcac cggcaagccc gagttcacca acctgaaggt
     1921 gtaccacgac atcaaggaca ttaccgcccg gaaagagatt attgagaacg ccgagctgct
     1981 ggatcagatt gccaagatcc tgaccatcta ccagagcagc gaggacatcc aggaagaact
     2041 gaccaatctg aactccgagc tgacccagga agagatcgag cagatctcta atctgaaggg
     2101 ctataccggc acccacaacc tgagcctgaa ggccatcaac ctgatcctgg acgagctgtg
     2161 gcacaccaac gacaaccaga tcgctatctt caaccggctg aagctggtgc ccaagaaggt
     2221 ggacctgtcc cagcagaaag agatccccac caccctggtg gacgacttca tcctgagccc
     2281 cgtcgtgaag agaagcttca tccagagcat caaagtgatc aacgccatca tcaagaagta
     2341 cggcctgccc aacgacatca ttatcgagct ggcccgcgag aagaactcca aggacgccca
     2401 gaaaatgatc aacgagatgc agaagcggaa cgcgaaaacc aacgagcgga tcgaggaaat
     2461 catccggacc accggcaaag agaacgccaa gtacctgatc gagaagatca agctgcacga
     2521 catgcaggaa ggcaagtgcc tgtacagcct ggaagccatc cctctggaag atctgctgaa
     2581 caaccccttc aactatgagg tggaccacat catccccaga agcgtgtcct tcgacaacag
     2641 cttcaacaac aaggtgctcg tgaagcagga agaaaacagc aagaagggca accggacccc
     2701 attccagtac ctgagcagca gcgacagcaa gatcagctac gaaaccttca agaagcacat
     2761 cctgaatctg gccaagggca agggcagaat cagcaagacc aagaaagagt atctgctgga
     2821 agaacgggac atcaacaggt tctccgtgca gaaagacttc atcaaccgga acctggtgga
     2881 taccagatac gccaccgcac gtctgatgaa cctgctgcgg agctacttca gagtgaacaa
     2941 cctggacgtg aaagtgaagt ccatcaatgg cggcttcacc agctttctgc ggcggaagtg
     3001 gaagtttaag aaagagcgga acaaggggta caagcaccac gccgaggacg ccctgatcat
     3061 tgccaacgcc gatttcatct tcaaagagtg gaagaaactg gacaaggcca aaaaagtgat
     3121 ggaaaaccag atgttcgagg aaaagcaggc cgagagcatg cccgagatcg aaaccgagca
     3181 ggagtacaaa gagatcttca tcacccccca ccagatcaag cacattaagg acttcaagga
     3241 ctacaagtac agccaccggg tggacaagaa gcctaataga gagctgatta acgacaccct
     3301 gtactccacc cggaaggacg acaagggcaa caccctgatc gtgaacaatc tgaacggcct
     3361 gtacgacaag gacaatgaca agctgaaaaa gctgatcaac aagagccccg aaaagctgct
     3421 gatgtaccac cacgaccccc agacctacca gaaactgaag ctgattatgg aacagtacgg
     3481 cgacgagaag aatcccctgt acaagtacta cgaggaaacc gggaactacc tgaccaagta
     3541 ctccaaaaag gacaacggcc ccgtgatcaa gaagattaag tattacggca acaaactgaa
     3601 cgcccatctg gacatcaccg acgactaccc caacagcaga aacaaggtcg tgaagctgtc
     3661 cctgaagccc tacagattcg acgtgtacct ggacaatggc gtgtacaagt tcgtgaccgt
     3721 gaagaatctg gatgtgatca aaaaagaaaa ctactacgaa gtgaatagca agtgctatga
     3781 ggaagctaag aagctgaaga agatcagcaa ccaggccgag tttatcgcct ccttctacaa
     3841 caacgatctg atcaagatca acggcgagct gtatagagtg atcggcgtga acaacgacct
     3901 gctgaaccgg atcgaagtga acatgatcga catcacctac cgcgagtacc tggaaaacat
     3961 gaacgacaag aggcccccca ggatcattaa gacaatcgcc tccaagaccc agagcattaa
     4021 gaagtacagc acagacattc tgggcaacct gtatgaagtg aaatctaaga agcaccctca
     4081 gatcatcaaa aagggcaaaa ggccggcggc cacgaaaaag gccggccagg caaaaaagaa
     4141 aaagggatcc tacccatacg atgttccaga ttacgcttac ccatacgatg ttccagatta
     4201 cgcttaccca tacgatgttc cagattacgc tggttcgatg gtgagcaagg gcgaggagct
     4261 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     4321 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     4381 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tcggctacgg
     4441 cctgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     4501 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     4561 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     4621 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     4681 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     4741 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     4801 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagctacc agtccgccct
     4861 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     4921 cgggatcact ctcggcatgg acgagctgta caagtgagct tgtcaagcag atcgttcaaa
     4981 catttggcaa taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat
     5041 ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt
     5101 tatgagatgg gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa
     5161 caaaatatag cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga
     5221 tcgacgctac tagaattcga gctcggagca tcttcattct taagatatga agataatctt
     5281 caaaaggccc ctgggaatct gaaagaagag aagcaggccc atttatatgg gaaagaacaa
     5341 tagtatttct tatataggcc catttaagtt gaaaacaatc ttcaaaagtc ccacatcgct
     5401 tagataagaa aacgaagctg agtttatata cagctagagt cgaagtagtg attgttcatg
     5461 gtgatcagga cttccgtttt agtactctgg aaacagaatc tactaaaaca aggcaaaatg
     5521 ccgtgtttat ctcgtcaact tgttggcgag atttttttac ttgtctcgct ttacgaattc
     5581 ccatggggag catcttcatt cttaagatat gaagataatc ttcaaaaggc ccctgggaat
     5641 ctgaaagaag agaagcaggc ccatttatat gggaaagaac aatagtattt cttatatagg
     5701 cccatttaag ttgaaaacaa tcttcaaaag tcccacatcg cttagataag aaaacgaagc
     5761 tgagtttata tacagctaga gtcgaagtag tgattgaagc ctaggaaact cttccttgtt
     5821 ttagtactct ggaaacagaa tctactaaaa caaggcaaaa tgccgtgttt atctcgtcaa
     5881 cttgttggcg agattttttt acttgtctcg ctcagagagg atgcacatgt gaccgaggga
     5941 cacgaagtga tccgtttaaa ctatcagtgt ttgacaggat atattggcgg gtaaacctaa
     6001 gagaaaagag cgtttattag aataatcgga tatttaaaag ggcgtgaaaa ggtttatccg
     6061 ttcgtccatt tgtatgtgca tgccaaccac agggttcccc tcgggagtca gccgtgcggc
     6121 tgcatgaaat cctggccggt ttgtctgatg ccaagctggc ggcctggccg gccagcttgg
     6181 ccgctgaaga aaccgagcgc cgccgtctaa aaaggtgatg tgtatttgag taaaacagct
     6241 tgcgtcatgc ggtcgctgcg tatatgatgc gatgagtaaa taaacaaata cgcaagggga
     6301 acgcatgaag gttatcgctg tacttaacca gaaaggcggg tcaggcaaga cgaccatcgc
     6361 aacccatcta gcccgcgccc tgcaactcgc cggggccgat gttctgttag tcgattccga
     6421 tccccagggc agtgcccgcg attgggcggc cgtgcgggaa gatcaaccgc taaccgttgt
     6481 cggcatcgac cgcccgacga ttgaccgcga cgtgaaggcc atcggccggc gcgacttcgt
     6541 agtgatcgac ggagcgcccc aggcggcgga cttggctgtg tccgcgatca aggcagccga
     6601 cttcgtgctg attccggtgc agccaagccc ttacgacata tgggccaccg ccgacctggt
     6661 ggagctggtt aagcagcgca ttgaggtcac ggatggaagg ctacaagcgg cctttgtcgt
     6721 gtcgcgggcg atcaaaggca cgcgcatcgg cggtgaggtt gccgaggcgc tggccgggta
     6781 cgagctgccc attcttgagt cccgtatcac gcagcgcgtg agctacccag gcactgccgc
     6841 cgccggcaca accgttcttg aatcagaacc cgagggcgac gctgcccgcg aggtccaggc
     6901 gctggccgct gaaattaaat caaaactcat ttgagttaat gaggtaaaga gaaaatgagc
     6961 aaaagcacaa acacgctaag tgccggccgt ccgagcgcac gcagcagcaa ggctgcaacg
     7021 ttggccagcc tggcagacac gccagccatg aagcgggtca actttcagtt gccggcggag
     7081 gatcacacca agctgaagat gtacgcggta cgccaaggca agaccattac cgagctgcta
     7141 tctgaataca tcgcgcagct accagagtaa atgagcaaat gaataaatga gtagatgaat
     7201 tttagcggct aaaggaggcg gcatggaaaa tcaagaacaa ccaggcaccg acgccgtgga
     7261 atgccccatg tgtggaggaa cgggcggttg gccaggcgta agcggctggg ttgtctgccg
     7321 gccctgcaat ggcactggaa cccccaagcc cgaggaatcg gcgtgagcgg tcgcaaacca
     7381 tccggcccgg tacaaatcgg cgcggcgctg ggtgatgacc tggtggagaa gttgaaggcc
     7441 gcgcaggccg cccagcggca acgcatcgag gcagaagcac gccccggtga atcgtggcaa
     7501 gcggccgctg atcgaatccg caaagaatcc cggcaaccgc cggcagccgg tgcgtcgtcg
     7561 attaggaagc cgcccaaggg cgacgagcaa ccagattttt tcgttccgat gctctatgac
     7621 gtgggcaccc gcgatagtcg cagcatcatg gacgtggccg ttttccgtct gtcgaagcgt
     7681 gaccgacgag ctggcgaggt gatccgctac gagcttccag acgggcacgt agaggtttcc
     7741 gcagggccgg ccggcatggc cagtgtgtgg gattacgacc tggtactgat ggcggtttcc
     7801 catctaaccg aatccatgaa ccgataccgg gaagggaagg gagacaagcc cggccgcgtg
     7861 ttccgtccac acgttgcgga cgtactcaag ttctgccggc gagccgatgg cggaaagcag
     7921 aaagacgacc tggtagaaac ctgcattcgg ttaaacacca cgcacgttgc catgcagcgt
     7981 acgaagaagg ccaagaacgg ccgcctggtg acggtatccg agggtgaagc cttgattagc
     8041 cgctacaaga tcgtaaagag cgaaaccggg cggccggagt acatcgagat cgagctagct
     8101 gattggatgt accgcgagat tacagaaggc aagaacccgg acgtgctgac ggttcacccc
     8161 gattactttt tgatcgatcc cggcatcggc cgttttctct accgcctggc acgccgcgcc
     8221 gcaggcaagg cagaagccag atggttgttc aagacgatct acgaacgcag tggcagcgcc
     8281 ggagagttca agaagttctg tttcaccgtg cgcaagctga tcgggtcaaa tgacctgccg
     8341 gagtacgatt tgaaggagga ggcggggcag gctggcccga tcctagtcat gcgctaccgc
     8401 aacctgatcg agggcgaagc atccgccggt tcctaatgta cggagcagat gctagggcaa
     8461 attgccctag caggggaaaa aggtcgaaaa agcttctttc ctgtggatag cacgtacatt
     8521 gggaacccaa agccgtacat tgggaaccgg aacccgtaca ttgggaaccc aaagccgtac
     8581 attgggaacc ggtcacacat gtaagtgact gatataaaag agaaaaaagg cgatttttcc
     8641 gcctaaaact ctttaaaact tattaaaact cttaaaaccc gcctggcctg tgcataactg
     8701 tctggccagc gcacagccga acagctgcaa aaagcgccca catcaaggct ccgagtgcgc
     8761 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     8821 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatggctaa aatgagaata
     8881 tcaccggaat tgaaaaaact gatcgaaaaa taccgctgcg taaaagatac ggaaggaatg
     8941 tctcctgcta aggtatataa gctggtggga gaaaatgaaa acctatattt aaaaatgacg
     9001 gacagccggt ataaagggac cacctatgat gtggaacggg aaaaggacat gatgctatgg
     9061 ctggaaggaa agctgcctgt tccaaaggtc ctgcactttg aacggcatga tggctggagc
     9121 aatctgctca tgagtgaggc cgatggcgtc ctttgctcgg aagagtatga agatgaacaa
     9181 agccctgaaa agattatcga gctgtatgcg gagtgcatca ggctctttca ctccatcgac
     9241 atatcggatt gtccctatac gaatagctta gacagccgct tagccgaatt ggattactta
     9301 ctgaataacg atctggccga tgtggattgc gaaaactggg aagaggacac tccatttaaa
     9361 gatccgcgcg agctgtatga ttttttaaag acggaaaagc ccgaagagga acttgtcttt
     9421 tcccacggcg acctgggaga cagcaacatc tttgtgaaag atggcaaagt aagtggcttt
     9481 attgatcttg ggagaagcgg cagggcggac aagtggtatg acattgcctt ctgcgtccgg
     9541 tcgctcaggg aggatatcgg ggaagaacag tatgtcgagc tattttttga cttactgggg
     9601 atcaagcctg attgggagaa aataaaatat tatattttac tggatgaatt gttttagctg
     9661 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     9721 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     9781 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     9841 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     9901 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     9961 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
    10021 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
    10081 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
    10141 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
    10201 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
    10261 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
    10321 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
    10381 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
    10441 cggttcctgc tcggatctgt tggaccggac agtagtcatg gttgatgggc tgcctgtatc
    10501 gagtggtgat tttgtgccga gctgccggtc ggggagctgt tggctggctg gtggcaggat
    10561 atattgtggt gtaaacaaat tgacgcttag acaacttaat aacacattgc ggacgttttt
    10621 aatgtactgg ggtt
//
