LOCUS       sequence-240254-       11696 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of PIK3CA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11696)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 11696)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-PIK3CA
FEATURES             Location/Qualifiers
     source          1..11696
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     99..115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     165..184
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             376..1009
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1056..1181
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1678..1911
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2096..2140
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2433..2550
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2598..3776
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2829..3767
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3724..3744
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3796..3820
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7031..7055)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        7063..7081
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7106..7125)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7107..7125)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(7131..7151)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             7205..7246
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    7267..7834
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    7284..7836
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7428..7445)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7655..7674
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7848..8447
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7848..7867)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8344..8364
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8461..9049
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8514..8534)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8932..8943)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9124..9357
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9476..9494)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9562..9666
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9667..9735,9736..10527)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9885..9904)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10698..11286
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11187..11206
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11440..11457
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgacatgat
      121 tacgaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
      181 catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc
      241 caaacttccc accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta
      301 aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac
      361 tacaaactta gtagttggaa gggctaattc actcccaaag aagacaagat atccttgatc
      421 tgtggatcta ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag
      481 gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata
      541 aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg
      601 ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc
      661 atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct
      721 acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt
      781 ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg
      841 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
      901 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
      961 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
     1021 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
     1081 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     1141 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     1201 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     1261 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     1321 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     1381 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1441 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1501 agaccaccgc acagcaagcg gccggccgct gatcttcaga cctggaggag gagatatgag
     1561 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1621 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1681 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     1741 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1801 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1861 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1921 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1981 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     2041 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     2101 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     2161 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     2221 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     2281 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     2341 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     2401 ccgaattcac aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
     2461 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
     2521 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
     2581 gtttggacta gtcgtgaggc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag
     2641 tccccgagaa gttgggggga ggggtcggca attgaaccgg tgcctagaga aggtggcgcg
     2701 gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag
     2761 aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca
     2821 gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg cctctttacg ggttatggcc
     2881 cttgcgtgcc ttgaattact tccacctggc tgcagtacgt gattcttgat cccgagcttc
     2941 gggttggaag tgggtgggag agttcgaggc cttgcgctta aggagcccct tcgcctcgtg
     3001 cttgagttga ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc
     3061 gcgcctgtct cgctgctttc gataagtctc tagccattta aaatttttga tgacctgctg
     3121 cgacgctttt tttctggcaa gatagtcttg taaatgcggg ccaagatctg cacactggta
     3181 tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg
     3241 cgaggcgggg cctgcgagcg cggccaccga gaatcggacg ggggtagtct caagctggcc
     3301 ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc
     3361 tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag
     3421 ggagctcaaa atggaggacg cggcgctcgg gagagcgggc gggtgagtca cccacacaaa
     3481 ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga ctccacggag taccgggcgc
     3541 cgtccaggca cctcgattag ttctcgagct tttggagtac gtcgtcttta ggttgggggg
     3601 aggggtttta tgcgatggag tttccccaca ctgagtgggt ggagactgaa gttaggccag
     3661 cttggcactt gatgtaattc tccttggaat ttgccctttt tgagtttgga tcttggttca
     3721 ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc atttcaggtg tcgtgaagcg
     3781 gccctgcaga tatcaacaag tttgtacaaa aaagcaggca ccatgcctcc aagaccatca
     3841 tcaggtgaac tgtggggcat ccacttgatg cccccaagaa tcctagtaga atgtttacta
     3901 ccaaatggaa tgatagtgac tttagaatgc ctccgtgagg ctacattaat aaccataaag
     3961 catgaactat ttaaagaagc aagaaaatac cccctccatc aacttcttca agatgaatct
     4021 tcttacattt tcgtaagtgt tactcaagaa gcagaaaggg aagaattttt tgatgaaaca
     4081 agacgacttt gtgaccttcg gctttttcaa ccctttttaa aagtaattga accagtaggc
     4141 aaccgtgaag aaaagatcct caatcgagaa attggttttg ctatcggcat gccagtgtgt
     4201 gaatttgata tggttaaaga tccagaagta caggacttcc gaagaaatat tctgaacgtt
     4261 tgtaaagaag ctgtggatct tagggacctc aattcacctc atagtagagc aatgtatgtc
     4321 tatcctccaa atgtagaatc ttcaccagaa ttgccaaagc acatatataa taaattagat
     4381 aaagggcaaa taatagtggt gatctgggta atagtttctc caaataatga caagcagaag
     4441 tatactctga aaatcaacca tgactgtgta ccagaacaag taattgctga agcaatcagg
     4501 aaaaaaactc gaagtatgtt gctatcctct gaacaactaa aactctgtgt tttagaatat
     4561 cagggcaagt atattttaaa agtgtgtgga tgtgatgaat acttcctaga aaaatatcct
     4621 ctgagtcagt ataagtatat aagaagctgt ataatgcttg ggaggatgcc caatttgatg
     4681 ttgatggcta aagaaagcct ttattctcaa ctgccaatgg actgttttac aatgccatct
     4741 tattccagac gcatttccac agctacacca tatatgaatg gagaaacatc tacaaaatcc
     4801 ctttgggtta taaatagtgc actcagaata aaaattcttt gtgcaaccta cgtgaatgta
     4861 aatattcgag acattgataa gatctatgtt cgaacaggta tctaccatgg aggagaaccc
     4921 ttatgtgaca atgtgaacac tcaaagagta ccttgttcca atcccaggtg gaatgaatgg
     4981 ctgaattatg atatatacat tcctgatctt cctcgtgctg ctcgactttg cctttccatt
     5041 tgctctgtta aaggccgaaa gggtgctaaa gaggaacact gtccattggc atggggaaat
     5101 ataaacttgt ttgattacac agacactcta gtatctggaa aaatggcttt gaatctttgg
     5161 ccagtacctc atggattaga agatttgctg aaccctattg gtgttactgg atcaaatcca
     5221 aataaagaaa ctccatgctt agagttggag tttgactggt tcagcagtgt ggtaaagttc
     5281 ccagatatgt cagtgattga agagcatgcc aattggtctg tatcccgaga agcaggattt
     5341 agctattccc acgcaggact gagtaacaga ctagctagag acaatgaatt aagggaaaat
     5401 gacaaagaac agctcaaagc aatttctaca cgagatcctc tctctgaaat cactgagcag
     5461 gagaaagatt ttctatggag tcacagacac tattgtgtaa ctatccccga aattctaccc
     5521 aaattgcttc tgtctgttaa atggaattct agagatgaag tagcccagat gtattgcttg
     5581 gtaaaagatt ggcctccaat caaacctgaa caggctatgg aacttctgga ctgtaattac
     5641 ccagatccta tggttcgagg ttttgctgtt cggtgcttgg aaaaatattt aacagatgac
     5701 aaactttctc agtatttaat tcagctagta caggtcctaa aatatgaaca atatttggat
     5761 aacttgcttg tgagattttt actgaagaaa gcattgacta atcaaaggat tgggcacttt
     5821 ttcttttggc atttaaaatc tgagatgcac aataaaacag ttagccagag gtttggcctg
     5881 cttttggagt cctattgtcg tgcatgtggg atgtatttga agcacctgaa taggcaagtc
     5941 gaggcaatgg aaaagctcat taacttaact gacattctca aacaggagaa gaaggatgaa
     6001 acacaaaagg tacagatgaa gtttttagtt gagcaaatga ggcgaccaga tttcatggat
     6061 gctctacagg gctttctgtc tcctctaaac cctgctcatc aactaggaaa cctcaggctt
     6121 gaagagtgtc gaattatgtc ctctgcaaaa aggccactgt ggttgaattg ggagaaccca
     6181 gacatcatgt cagagttact gtttcagaac aatgagatca tctttaaaaa tggggatgat
     6241 ttacggcaag atatgctaac acttcaaatt attcgtatta tggaaaatat ctggcaaaat
     6301 caaggtcttg atcttcgaat gttaccttat ggttgtctgt caatcggtga ctgtgtggga
     6361 cttattgagg tggtgcgaaa ttctcacact attatgcaaa ttcagtgcaa aggcggcttg
     6421 aaaggtgcac tgcagttcaa cagccacaca ctacatcagt ggctcaaaga caagaacaaa
     6481 ggagaaatat atgatgcagc cattgacctg tttacacgtt catgtgctgg atactgtgta
     6541 gctaccttca ttttgggaat tggagatcgt cacaatagta acatcatggt gaaagacgat
     6601 ggacaactgt ttcatataga ttttggacac tttttggatc acaagaagaa aaaatttggt
     6661 tataaacgag aacgtgtgcc atttgttttg acacaggatt tcttaatagt gattagtaaa
     6721 ggagcccaag aatgcacaaa gacaagagaa tttgagaggt ttcaggagat gtgttacaag
     6781 gcttatctag ctattcgaca gcatgccaat ctcttcataa atcttttctc aatgatgctt
     6841 ggctctggaa tgccagaact acaatctttt gatgacattg catacattcg aaagacccta
     6901 gccttagata aaactgagca agaggctttg gagtatttca tgaaacaaat gaatgatgca
     6961 catcatggtg gctggacaac aaaaatggat tggatcttcc acacaattaa acagcatgca
     7021 ttgaactaga acccagcttt cttgtacaaa gtggtgatat ccaattaacc ctcactaaag
     7081 gagtagaaag ttgaaattga ttatgcccta tagtgagtcg tattaccacc caacttttct
     7141 atacaaagtg gttgatatcc agcacagtgg cggccgctcg agtctagagg gcccgcggtt
     7201 cgaaggtaag cctatcccta accctctcct cggtctcgat tctacgcgta ccggttagta
     7261 atgagatccc tccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg
     7321 tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg
     7381 gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg
     7441 aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca
     7501 aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct
     7561 ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca
     7621 cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa
     7681 ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg
     7741 cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg
     7801 ggacgtggtt ttcctttgaa aaacacgatg ataatatggc cacacatatg accgagtaca
     7861 agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg
     7921 ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc
     7981 gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt
     8041 gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg
     8101 gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg
     8161 cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc
     8221 tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc
     8281 tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc
     8341 cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc
     8401 ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctaaatc gatagatcct
     8461 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     8521 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     8581 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     8641 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     8701 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     8761 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     8821 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     8881 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     8941 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     9001 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tgagatcctt
     9061 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     9121 gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt
     9181 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     9241 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     9301 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
     9361 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     9421 gtgagaggcc cgggttaatt aaggaaaggg ctagatcatt cttgaagacg aaagggcctc
     9481 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     9541 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     9601 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     9661 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     9721 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     9781 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     9841 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     9901 ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     9961 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
    10021 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
    10081 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
    10141 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
    10201 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
    10261 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
    10321 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
    10381 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
    10441 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
    10501 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
    10561 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
    10621 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
    10681 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
    10741 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
    10801 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
    10861 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
    10921 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
    10981 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
    11041 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
    11101 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
    11161 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
    11221 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
    11281 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
    11341 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
    11401 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
    11461 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
    11521 agcaagctca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
    11581 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
    11641 tccccgtggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaat
//
