LOCUS       sequence-240259-       13316 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of MAP3K4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13316)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 13316)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-MAP3K4
FEATURES             Location/Qualifiers
     source          1..13316
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     99..115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     165..184
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             376..1009
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1056..1181
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1678..1911
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2096..2140
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2433..2550
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2598..3776
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2829..3767
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3724..3744
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3796..3820
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      3880..3889
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     RBS             5787..5795
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     protein_bind    complement(8651..8675)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        8683..8701
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8726..8745)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8727..8745)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(8751..8771)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             8825..8866
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    8887..9454
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    8904..9456
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(9048..9065)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     9275..9294
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             9468..10067
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9468..9487)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9964..9984
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10081..10669
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10134..10154)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10552..10563)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10744..10977
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(11096..11114)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11182..11286
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11287..11355,11356..12147)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11505..11524)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12318..12906
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12807..12826
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13060..13077
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgacatgat
      121 tacgaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
      181 catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc
      241 caaacttccc accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta
      301 aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac
      361 tacaaactta gtagttggaa gggctaattc actcccaaag aagacaagat atccttgatc
      421 tgtggatcta ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag
      481 gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata
      541 aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg
      601 ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc
      661 atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct
      721 acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt
      781 ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg
      841 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
      901 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
      961 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
     1021 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
     1081 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     1141 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     1201 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     1261 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     1321 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     1381 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1441 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1501 agaccaccgc acagcaagcg gccggccgct gatcttcaga cctggaggag gagatatgag
     1561 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1621 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1681 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     1741 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1801 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1861 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1921 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1981 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     2041 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     2101 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     2161 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     2221 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     2281 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     2341 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     2401 ccgaattcac aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
     2461 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
     2521 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
     2581 gtttggacta gtcgtgaggc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag
     2641 tccccgagaa gttgggggga ggggtcggca attgaaccgg tgcctagaga aggtggcgcg
     2701 gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag
     2761 aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca
     2821 gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg cctctttacg ggttatggcc
     2881 cttgcgtgcc ttgaattact tccacctggc tgcagtacgt gattcttgat cccgagcttc
     2941 gggttggaag tgggtgggag agttcgaggc cttgcgctta aggagcccct tcgcctcgtg
     3001 cttgagttga ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc
     3061 gcgcctgtct cgctgctttc gataagtctc tagccattta aaatttttga tgacctgctg
     3121 cgacgctttt tttctggcaa gatagtcttg taaatgcggg ccaagatctg cacactggta
     3181 tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg
     3241 cgaggcgggg cctgcgagcg cggccaccga gaatcggacg ggggtagtct caagctggcc
     3301 ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc
     3361 tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag
     3421 ggagctcaaa atggaggacg cggcgctcgg gagagcgggc gggtgagtca cccacacaaa
     3481 ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga ctccacggag taccgggcgc
     3541 cgtccaggca cctcgattag ttctcgagct tttggagtac gtcgtcttta ggttgggggg
     3601 aggggtttta tgcgatggag tttccccaca ctgagtgggt ggagactgaa gttaggccag
     3661 cttggcactt gatgtaattc tccttggaat ttgccctttt tgagtttgga tcttggttca
     3721 ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc atttcaggtg tcgtgaagcg
     3781 gccctgcaga tatcaacaag tttgtacaaa aaagcaggct tcatgagaga agccgctgcc
     3841 gcgctggtcc ctcctcccgc ctttgccgtc acgcctgccg ccgccatgga ggagccgccg
     3901 ccaccgccgc cgccgccacc accgccaccg gaacccgaga ccgagtcaga acccgagtgc
     3961 tgcttggcgg cgaggcaaga gggcacattg ggagattcag cttgcaagag tcctgaatct
     4021 gatctagaag acttctccga tgaaacaaat acagagaatc tttatggtac ctctcccccc
     4081 agcacacctc gacagatgaa acgcatgtca accaaacatc agaggaataa tgtggggagg
     4141 ccagccagtc ggtctaattt gaaagaaaaa atgaatgcac caaatcagcc tccacataaa
     4201 gacactggaa aaacagtgga gaatgtggaa gaatacagct ataagcagga gaaaaagatc
     4261 cgagcagctc ttagaacaac agagcgtgat cataaaaaaa atgtacagtg ctcattcatg
     4321 ttagactcag tgggtggatc tttgccaaaa aaatcaattc cagatgtgga tctcaataag
     4381 ccttacctca gccttggctg tagcaatgct aagcttccag tatctgtgcc catgcctata
     4441 gccagacctg cacgccagac ttctaggact gactgtccag cagatcgttt aaagtttttt
     4501 gaaactttac gacttttgct aaagcttacc tcagtctcaa agaaaaaaga cagggagcaa
     4561 agaggacaag aaaatacgtc tggtttctgg cttaaccgat ctaacgaact gatctggtta
     4621 gagctacaag cctggcatgc aggacggaca attaacgacc aggacttctt tttatataca
     4681 gcccgtcaag ccatcccaga tattattaat gaaatcctta ctttcaaagt cgactatggg
     4741 agcttcgcct ttgttagaga tagagctggt tttaatggta cttcagtaga agggcagtgc
     4801 aaagccactc ctggaacaaa gattgtaggt tactcaacac atcatgagca tctccaacgc
     4861 cagagggtct catttgagca ggtaaaacgg ataatggagc tgctagagta catagaagca
     4921 ctttatccat cattgcaggc tcttcagaag gactatgaaa aatatgctgc aaaagacttc
     4981 caggacaggg tgcaggcact ctgtttgtgg ttaaacatca caaaagactt aaatcagaaa
     5041 ttaaggatta tgggcactgt tttgggcatc aagaatttat cagacattgg ctggccagtg
     5101 tttgaaatcc cttcccctcg accatccaaa ggtaatgagc cggagtatga gggtgatgac
     5161 acagaaggag aattaaagga gttggaaagt agtacggatg agagtgaaga agaacaaatc
     5221 tctgatccta gggtaccgga aatcagacag cccatagata acagcttcga catccagtcg
     5281 cgggactgca tatccaagaa gcttgagagg ctcgaatctg aggatgattc tcttggctgg
     5341 ggagcaccag actggagcac agaagcaggc tttagtagac attgtctgac ttctatttat
     5401 agaccatttg tagacaaagc actgaagcag atggggttaa gaaagttaat tttaagactt
     5461 cacaagctaa tggatggttc cttgcaaagg gcacgtatag cattggtaaa gaacgatcgt
     5521 ccagtggagt tttctgaatt tccagatccc atgtggggtt cagattatgt gcagttgtca
     5581 aggacaccac cttcatctga ggagaaatgc agtgctgtgt cgtgggagga gctgaaggcc
     5641 atggatttac cttcattcga acctgccttc ctagttctct gccgagtcct tctgaatgtc
     5701 atacatgagt gtctgaagtt aagattggag cagagacctg ctggagaacc atctctcttg
     5761 agtattaagc agctggtgag agagtgtaag gaggtcctga agggcggcct gctgatgaag
     5821 cagtactacc agttcatgct gcaggaggtt ctggaggact tggagaagcc cgactgcaac
     5881 attgacgctt ttgaagagga tctacataaa atgcttatgg tgtattttga ttacatgaga
     5941 agctggatcc aaatgctaca gcaattacct caagcatcgc atagtttaaa aaatctgtta
     6001 gaagaagaat ggaatttcac caaagaaata actcattaca tacggggagg agaagcacag
     6061 gccgggaagc ttttctgtga cattgcagga atgctgctga aatctacagg aagtttttta
     6121 gaatttggct tacaggagag ctgtgctgaa ttttggacta gtgcggatga cagcagtgct
     6181 tccgacgaaa tcaggaggtc tgttatagag atcagtcgag ccctgaagga gctcttccat
     6241 gaagccagag aaagggcttc caaagcactt ggatttgcta aaatgttgag aaaggacctg
     6301 gaaatagcag cagaattcag gctttcagcc ccagttagag acctcctgga tgttctgaaa
     6361 tcaaaacagt atgtcaaggt gcaaattcct gggttagaaa acttgcaaat gtttgttcca
     6421 gacactcttg ctgaggagaa gagtattatt ttgcagttac tcaatgcagc tgcaggaaag
     6481 gactgttcaa aagattcaga tgacgtactc atcgatgcct atctgcttct gaccaagcac
     6541 ggtgatcgag cccgtgattc agaggacagc tggggcacct gggaggcaca gcctgtcaaa
     6601 gtcgtgcctc aggtggagac tgttgacacc ctgagaagca tgcaggtgga taatctttta
     6661 ctagttgtca tgcagtctgc gcatctcaca attcagagaa aagctttcca gcagtccatt
     6721 gagggactta tgactctgtg ccaggagcag acatccagtc agccggtcat cgccaaagct
     6781 ttgcagcagc tgaagaatga tgcattggag ctatgcaaca ggataagcaa tgccattgac
     6841 cgcgtggacc acatgttcac atcagaattt gatgctgagg ttgatgaatc tgaatctgtc
     6901 accttgcaac agtactaccg agaagcaatg attcaggggt acaattttgg atttgagtat
     6961 cataaagaag ttgttcgttt gatgtctggg gagtttagac agaagatagg agacaaatat
     7021 ataagctttg cccggaagtg gatgaattat gtcctgacta aatgtgagag tggtagaggt
     7081 acaagaccca ggtgggcgac tcaaggattt gattttctac aagcaattga acctgccttt
     7141 atttcagctt taccagaaga tgacttcttg agtttacaag ccttgatgaa tgaatgcatt
     7201 ggccatgtca taggaaaacc acacagtcct gttacaggtt tgtaccttgc cattcatcgg
     7261 aacagccccc gtcctatgaa ggtacctcga tgccatagtg accctcctaa cccacacctc
     7321 attatcccca ctccagaggg attcagcact cggagcatgc cttccgacgc gcggagccat
     7381 ggcagccctg ctgctgctgc tgctgctgct gctgctgctg ttgctgccag tcggcccagc
     7441 ccctctggtg gtgactctgt gctgcccaaa tccatcagca gtgcccatga taccaggggt
     7501 tccagcgttc ctgaaaatga tcgattggct tccatagctg ctgaattgca gtttaggtcc
     7561 ctgagtcgtc actcaagccc cacggaggag cgagatgaac cagcatatcc aagaggagat
     7621 tcaagtgggt ccacaagaag aagttgggaa cttcggacac taatcagcca gagtaaagat
     7681 actgcttcta aactaggacc catagaagct atccagaagt cagtccgatt gtttgaagaa
     7741 aagaggtacc gagaaatgag gagaaagaat atcattggtc aagtttgtga tacgcctaag
     7801 tcctatgata atgttatgca cgttggcttg aggaaggtga ccttcaaatg gcaaagagga
     7861 aacaaaattg gagaaggcca gtatgggaag gtgtacacct gcatcagcgt cgacaccggg
     7921 gagctgatgg ccatgaaaga gattcgattt caacctaatg accataagac tatcaaggaa
     7981 actgcagacg aattgaaaat attcgaaggc atcaaacacc ccaatctggt tcggtatttt
     8041 ggtgtggagc tccatagaga agaaatgtac atcttcatgg agtactgcga tgaggggact
     8101 ttagaagagg tgtcaaggct gggacttcag gaacatgtga ttaggctgta ttcaaagcag
     8161 atcaccattg cgatcaacgt cctccatgag catggcatag tccaccgtga cattaaaggt
     8221 gccaatatct tccttacctc atctggatta atcaaactgg gagattttgg atgttcagta
     8281 aagctcaaaa acaatgccca gaccatgcct ggtgaagtga acagcaccct ggggacagca
     8341 gcatacatgg cacctgaagt catcactcgt gccaaaggag agggccatgg gcgtgcggcc
     8401 gacatctgga gtctggggtg tgttgtcata gagatggtga ctggcaagag gccttggcat
     8461 gagtatgagc acaactttca aattatgtat aaagtgggga tgggacataa gccaccaatc
     8521 cctgaaagat taagccctga aggaaaggac ttcctttctc actgccttga gagtgaccca
     8581 aagatgagat ggaccgccag ccagctcctc gaccattcgt ttgtcaaggt ttgcacagat
     8641 gaagaatgag acccagcttt cttgtacaaa gtggtgatat ccaattaacc ctcactaaag
     8701 gagtaagaat gtaatatgat gatagcccta tagtgagtcg tattaccacc caacttttct
     8761 atacaaagtg gttgatatcc agcacagtgg cggccgctcg agtctagagg gcccgcggtt
     8821 cgaaggtaag cctatcccta accctctcct cggtctcgat tctacgcgta ccggttagta
     8881 atgagatccc tccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg
     8941 tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg
     9001 gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg
     9061 aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca
     9121 aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct
     9181 ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca
     9241 cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa
     9301 ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg
     9361 cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg
     9421 ggacgtggtt ttcctttgaa aaacacgatg ataatatggc cacacatatg accgagtaca
     9481 agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg
     9541 ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc
     9601 gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt
     9661 gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg
     9721 gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg
     9781 cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc
     9841 tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc
     9901 tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc
     9961 cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc
    10021 ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctaaatc gatagatcct
    10081 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
    10141 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
    10201 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
    10261 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
    10321 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
    10381 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
    10441 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
    10501 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
    10561 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
    10621 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tgagatcctt
    10681 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
    10741 gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt
    10801 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
    10861 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
    10921 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
    10981 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
    11041 gtgagaggcc cgggttaatt aaggaaaggg ctagatcatt cttgaagacg aaagggcctc
    11101 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
    11161 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
    11221 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
    11281 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
    11341 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
    11401 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
    11461 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
    11521 ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
    11581 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
    11641 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
    11701 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
    11761 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
    11821 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
    11881 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
    11941 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
    12001 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
    12061 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
    12121 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
    12181 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
    12241 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
    12301 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
    12361 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
    12421 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
    12481 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
    12541 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
    12601 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
    12661 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
    12721 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
    12781 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
    12841 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
    12901 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
    12961 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
    13021 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
    13081 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
    13141 agcaagctca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
    13201 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
    13261 tccccgtggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaat
//
