LOCUS       sequence-240261-        8491 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression plasmid of human POU2F3 cDNA (CRISPR-resistant
            silent mutation) with neomycin resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8491)
  AUTHORS   Huang YH, Klingbeil O, He XY, Wu XS, Arun G, Lu B, Somerville TDD, 
            Milazzo JP, Wilkinson JE, Demerdash OE, Spector DL, Egeblad M, Shi 
            J, Vakoc CR
  TITLE     POU2F3 is a master regulator of a tuft cell-like variant of small 
            cell lung cancer.
  JOURNAL   Genes Dev. 2018 Jul 1;32(13-14):915-928. doi: 
            10.1101/gad.314815.118. Epub 2018 Jun 26.
  PUBMED    29945888
REFERENCE   2  (bases 1 to 8491)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            Dev."; date: "2018-07-1"; volume: "32(13-14):915-928. doi"; pages: "
            10.1101/gad.314815.118. Epub 2018 Jun 26"
COMMENT     Sequence Label: LentiV_Neo_POU2F3_CR
FEATURES             Location/Qualifiers
     source          1..8491
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1723..1840
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1909..2120
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      2140..2149
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3463..3519
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3538..4332
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3592..3611)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4202..4221
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    4348..4936
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4401..4421)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4819..4830)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5008..5241
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5319..5440
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5356..5375)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5410..5429
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5480..5615
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5635..5654)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5636..5654)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5664..5681)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5664..5680)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5673..5695)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5822..6277
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5909..5928)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6119..6140
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6303..6407
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6408..6476,6477..7268)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6626..6645)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7439..8027
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7928..7947
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8181..8198
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8315..8336
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8351..8356,8357..8374,8375..8381)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8389..8405
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8394..8416
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8413..8429
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8413..8429
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8448..8468
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8450..8468
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccagaccac aattttaaaa gaaaaggggg
     1741 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     1801 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     1861 cagagatcca ctttggcgcc ggctcgagtg gctccggtgc ccgtcagtgg gcagagcgca
     1921 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga
     1981 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
     2041 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
     2101 ggtttgccgc cagaacacag gtgtcgtgac gcgggatccg ccaccatggt gaatctggag
     2161 tccatgcaca cagatatcaa gatgagtggg gatgtagccg attccacgga tgctcgcagc
     2221 actctcagcc aggtggagcc aggaaatgat cgaaatggcc tagatttcaa caggcagatt
     2281 aaaaccgaag atctcagtga ctccctgcag cagaccctct cccatcggcc atgccacctg
     2341 agtcaaggac ctgccatgat gtccggaaac caaatgtctg ggctaaatgc cagcccatgt
     2401 caggacatgg cttccctcca tccgctccag cagcttgtgc tggttcccgg ccacttacag
     2461 tctgtatccc agttcctgct atctcagacc cagcctgggc agcaaggtct gcagccaaat
     2521 ctcctcccct ttccacagca acaaagcggt ctcctcctcc cacagactgg gccgggactg
     2581 gcatcccagg catttgggca ccctgggctg ccaggatcct ctttagaacc ccacctggaa
     2641 gcatcccagc atctcccagt gcccaagcat ctacccagct ctggaggggc cgatgagccc
     2701 agtgacctcg aggagctgga gaagtttgcc aagaccttca agcagaggcg cattaagctg
     2761 ggcttcacac agggagatgt ggggctggcg atgggaaagc tgtatggcaa cgacttcagc
     2821 cagaccacca tctcacgatt tgaggccctc aacctgagct tcaagaacat gtgcaagctc
     2881 aagcccctgc tggagaagtg gctgaatgat gcagagtcct ctccgtcaga cccctcagtg
     2941 agcacgccca gctcctaccc cagcctcagt gaagtatttg gtaggaagag aaagaaacgg
     3001 accagcatcg agaccaacat ccgcctcacc ctcgagaaga ggtttcaaga taacccaaaa
     3061 cccagctcgg aggagatctc catgattgca gagcagttgt ccatggagaa ggaggtggtg
     3121 agggtctggt tctgcaaccg acgccaaaag gagaagcgaa tcaactgccc tgtggccaca
     3181 cccatcaaac cacctgtcta caactcccgg ctggtatctc cctcagggtc tctgggcccc
     3241 ctctctgtcc ctcctgtcca cagtaccatg cctggaacag taacgtcatc ctgttcccct
     3301 gggaacaaca gcaggccttc atctcctggc tcaggactcc acgccagcag ccccactgca
     3361 tctcaaaata actccaaagc agcagtgaac tccgcctcca gttttaactc ttcaggatct
     3421 tggtaccgat ggaatcattc cacctacctc cacggcagcg gcgccaccaa cttcagcctg
     3481 ctgaagcagg ccggcgacgt ggaggagaac cccggcccca tgaccgagta caagcccatg
     3541 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
     3601 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
     3661 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag
     3721 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
     3781 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
     3841 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
     3901 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
     3961 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
     4021 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc
     4081 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
     4141 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
     4201 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
     4261 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
     4321 gagttcttct gaacgcgtta agtcgacaat caacctctgg attacaaaat ttgtgaaaga
     4381 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     4441 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     4501 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     4561 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     4621 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     4681 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     4741 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     4801 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     4861 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     4921 tgggccgcct ccccgcgtcg actttaagac caatgactta caaggcagct gtagatctta
     4981 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     5041 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     5101 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     5161 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     5221 cagtgtggaa aatctctagc agtacgtata gtagttcatg tcatcttatt attcagtatt
     5281 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata
     5341 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     5401 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat
     5461 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     5521 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     5581 attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat
     5641 agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     5701 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     5761 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     5821 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     5881 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     5941 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     6001 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     6061 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     6121 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     6181 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     6241 aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg
     6301 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     6361 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     6421 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     6481 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     6541 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     6601 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     6661 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     6721 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     6781 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     6841 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     6901 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     6961 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     7021 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     7081 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     7141 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     7201 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     7261 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     7321 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     7381 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     7441 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     7501 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     7561 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     7621 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     7681 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     7741 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     7801 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     7861 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     7921 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     7981 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     8041 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     8101 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     8161 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     8221 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     8281 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     8341 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     8401 aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc
     8461 actaaaggga acaaaagctg gagctgcaag c
//
