LOCUS       sequence-240262-        9757 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of IDH1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9757)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 9757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-IDH1
FEATURES             Location/Qualifiers
     source          1..9757
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     99..115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     165..184
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             376..1009
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1056..1181
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1678..1911
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2096..2140
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2433..2550
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2598..3776
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2829..3767
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3724..3744
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3796..3820
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5069..5093)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5146..5187
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5208..5775
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5225..5777
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5369..5386)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5596..5615
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(5789..5791,5792..5794,5795..6508)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5789..5791,5792..5794,5795..6505)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5834..5855)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6095..6114)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6442..6463
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6522..7110
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6575..6595)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6993..7004)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7185..7418
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7537..7555)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7623..7727
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7728..7796,7797..8588)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7946..7965)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8759..9347
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9248..9267
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9501..9518
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgacatgat
      121 tacgaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
      181 catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc
      241 caaacttccc accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta
      301 aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac
      361 tacaaactta gtagttggaa gggctaattc actcccaaag aagacaagat atccttgatc
      421 tgtggatcta ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag
      481 gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata
      541 aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg
      601 ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc
      661 atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct
      721 acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt
      781 ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg
      841 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
      901 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
      961 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
     1021 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
     1081 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     1141 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     1201 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     1261 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     1321 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     1381 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1441 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1501 agaccaccgc acagcaagcg gccggccgct gatcttcaga cctggaggag gagatatgag
     1561 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1621 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1681 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     1741 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1801 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1861 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1921 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1981 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     2041 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     2101 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     2161 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     2221 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     2281 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     2341 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     2401 ccgaattcac aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
     2461 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
     2521 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
     2581 gtttggacta gtcgtgaggc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag
     2641 tccccgagaa gttgggggga ggggtcggca attgaaccgg tgcctagaga aggtggcgcg
     2701 gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag
     2761 aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca
     2821 gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg cctctttacg ggttatggcc
     2881 cttgcgtgcc ttgaattact tccacctggc tgcagtacgt gattcttgat cccgagcttc
     2941 gggttggaag tgggtgggag agttcgaggc cttgcgctta aggagcccct tcgcctcgtg
     3001 cttgagttga ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc
     3061 gcgcctgtct cgctgctttc gataagtctc tagccattta aaatttttga tgacctgctg
     3121 cgacgctttt tttctggcaa gatagtcttg taaatgcggg ccaagatctg cacactggta
     3181 tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg
     3241 cgaggcgggg cctgcgagcg cggccaccga gaatcggacg ggggtagtct caagctggcc
     3301 ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc
     3361 tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag
     3421 ggagctcaaa atggaggacg cggcgctcgg gagagcgggc gggtgagtca cccacacaaa
     3481 ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga ctccacggag taccgggcgc
     3541 cgtccaggca cctcgattag ttctcgagct tttggagtac gtcgtcttta ggttgggggg
     3601 aggggtttta tgcgatggag tttccccaca ctgagtgggt ggagactgaa gttaggccag
     3661 cttggcactt gatgtaattc tccttggaat ttgccctttt tgagtttgga tcttggttca
     3721 ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc atttcaggtg tcgtgaagcg
     3781 gccctgcaga tatcaacaag tttgtacaaa aaagcaggca ccatgtccaa aaaaatcagt
     3841 ggcggttctg tggtagagat gcaaggagat gaaatgacac gaatcatttg ggaattgatt
     3901 aaagagaaac tcatttttcc ctacgtggaa ttggatctac atagctatga tttaggcata
     3961 gagaatcgtg atgccaccaa cgaccaagtc accaaggatg ctgcagaagc tataaagaag
     4021 cataatgttg gcgtcaaatg tgccactatc actcctgatg agaagagggt tgaggagttc
     4081 aagttgaaac aaatgtggaa atcaccaaat ggcaccatac gaaatattct gggtggcacg
     4141 gtcttcagag aagccattat ctgcaaaaat atcccccggc ttgtgagtgg atgggtaaaa
     4201 cctatcatca taggtcgtca tgcttatggg gatcaataca gagcaactga ttttgttgtt
     4261 cctgggcctg gaaaagtaga gataacctac acaccaagtg acggaaccca aaaggtgaca
     4321 tacctggtac ataactttga agaaggtggt ggtgttgcca tggggatgta taatcaagat
     4381 aagtcaattg aagattttgc acacagttcc ttccaaatgg ctctgtctaa gggttggcct
     4441 ttgtatctga gcaccaaaaa cactattctg aagaaatatg atgggcgttt taaagacatc
     4501 tttcaggaga tatatgacaa gcagtacaag tcccagtttg aagctcaaaa gatctggtat
     4561 gagcataggc tcatcgacga catggtggcc caagctatga aatcagaggg aggcttcatc
     4621 tgggcctgta aaaactatga tggtgacgtg cagtcggact ctgtggccca agggtatggc
     4681 tctctcggca tgatgaccag cgtgctggtt tgtccagatg gcaagacagt agaagcagag
     4741 gctgcccacg ggactgtaac ccgtcactac cgcatgtacc agaaaggaca ggagacgtcc
     4801 accaatccca ttgcttccat ttttgcctgg accagagggt tagcccacag agcaaagctt
     4861 gataacaata aagagcttgc cttctttgca aatgctttgg aagaagtctc tattgagaca
     4921 attgaggctg gcttcatgac caaggacttg gctgcttgca ttaaaggttt acccaatgtg
     4981 caacgttctg actacttgaa tacatttgag ttcatggata aacttggaga aaacttgaag
     5041 atcaaactag ctcaggccaa actttagaac ccagctttct tgtacaaagt ggttgatatc
     5101 cagcacagtg gcggccgctc gagtctagag ggcccgcggt tcgaaggtaa gcctatccct
     5161 aaccctctcc tcggtctcga ttctacgcgt accggttagt aatgagatcc ctcccccccc
     5221 cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta
     5281 ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc
     5341 ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat
     5401 gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc
     5461 ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt
     5521 gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt
     5581 gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag
     5641 aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt
     5701 tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga
     5761 aaaacacgat gataatatgg ccacacatat ggtgagcaag ggcgaggagc tgttcaccgg
     5821 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
     5881 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
     5941 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg
     6001 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
     6061 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
     6121 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
     6181 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
     6241 ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa
     6301 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
     6361 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga
     6421 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
     6481 tctcggcatg gacgagctgt acaagtaaat cgatagatcc taatcaacct ctggattaca
     6541 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     6601 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     6661 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     6721 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     6781 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     6841 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     6901 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga
     6961 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     7021 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     7081 gtcggatctc cctttgggcc gcctccccgc ctgagatcct ttaagaccaa tgacttacaa
     7141 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     7201 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     7261 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     7321 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     7381 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
     7441 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagaggc ccgggttaat
     7501 taaggaaagg gctagatcat tcttgaagac gaaagggcct cgtgatacgc ctatttttat
     7561 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     7621 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     7681 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     7741 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     7801 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     7861 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     7921 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg
     7981 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     8041 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     8101 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     8161 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     8221 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     8281 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     8341 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     8401 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     8461 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     8521 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     8581 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     8641 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     8701 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     8761 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     8821 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     8881 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     8941 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     9001 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     9061 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     9121 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     9181 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     9241 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     9301 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     9361 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     9421 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     9481 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     9541 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagcaagctc atggctgact
     9601 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     9661 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctccccgtgg cacgacaggt
     9721 ttcccgactg gaaagcgggc agtgagcgca acgcaat
//
