LOCUS       sequence-240267-       11161 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of CDH1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11161)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 11161)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-CDH1
FEATURES             Location/Qualifiers
     source          1..11161
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6098..6122)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6175..6216
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    6237..6804
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    6254..6806
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6398..6415)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6625..6644
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(6818..6820,6821..6823,6824..7537)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6818..6820,6821..6823,6824..7534)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6863..6884)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7124..7143)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7471..7492
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    7551..8139
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7604..7624)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8022..8033)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8214..8447
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8566..8584)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8652..8756
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8757..8825,8826..9617)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8975..8994)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9788..10376
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10277..10296
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10530..10547
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10787..10808
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10823..10828,10829..10846,10847..10853)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10861..10877
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10866..10888
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10885..10901
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10885..10901
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10951..10970
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagc aggcaccatg ggcccttgga gccgcagcct ctcggcgctg
     3481 ctgctgctgc tgcaggtctc ctcttggctc tgccaggagc cggagccctg ccaccctggc
     3541 tttgacgccg agagctacac gttcacggtg ccccggcgcc acctggagag aggccgcgtc
     3601 ctgggcagag tgaattttga agattgcacc ggtcgacaaa ggacagccta tttttccctc
     3661 gacacccgat tcaaagtggg cacagatggt gtgattacag tcaaaaggcc tctacggttt
     3721 cataacccac agatccattt cttggtctac gcctgggact ccacctacag aaagttttcc
     3781 accaaagtca cgctgaatac agtggggcac caccaccgcc ccccgcccca tcaggcctcc
     3841 gtttctggaa tccaagcaga attgctcaca tttcccaact cctctcctgg cctcagaaga
     3901 cagaagagag actgggttat tcctcccatc agctgcccag aaaatgaaaa aggcccattt
     3961 cctaaaaacc tggttcagat caaatccaac aaagacaaag aaggcaaggt tttctacagc
     4021 atcactggcc aaggagctga cacaccccct gttggtgtct ttattattga aagagaaaca
     4081 ggatggctga aggtgacaga gcctctggat agagaacgca ttgccacata cactctcttc
     4141 tctcacgctg tgtcatccaa cgggaatgca gttgaggatc caatggagat tttgatcacg
     4201 gtaaccgatc agaatgacaa caagcccgaa ttcacccagg aggtctttaa ggggtctgtc
     4261 atggaaggtg ctcttccagg aacctctgtg atggaggtca cagccacaga cgcggacgat
     4321 gatgtgaaca cctacaatgc cgccatcgct tacaccatcc tcagccaaga tcctgagctc
     4381 cctgacaaaa atatgttcac cattaacagg aacacaggag tcatcagtgt ggtcaccact
     4441 gggctggacc gagagagttt ccctacgtat accctggtgg ttcaagctgc tgaccttcaa
     4501 ggtgaggggt taagcacaac agcaacagct gtgatcacag tcactgacac caacgataat
     4561 cctccgatct tcaatcccac cacgtacaag ggtcaggtgc ctgagaacga ggctaacgtc
     4621 gtaatcacca cactgaaagt gactgatgct gatgccccca ataccccagc gtgggaggct
     4681 gtatacacca tattgaatga tgatggtgga caatttgtcg tcaccacaaa tccagtgaac
     4741 aacgatggca ttttgaaaac agcaaagggc ttggattttg aggccaagca gcagtacatt
     4801 ctacacgtag cagtgacgaa tgtggtacct tttgaggtct ctctcaccac ctccacagcc
     4861 accgtcaccg tggatgtgct ggatgtgaat gaagccccca tctttgtgcc tcctgaaaag
     4921 agagtggaag tgtccgagga ctttggcgtg ggccaggaaa tcacatccta cactgcccag
     4981 gagccagaca catttatgga acagaaaata acatatcgga tttggagaga cactgccaac
     5041 tggctggaga ttaatccgga cactggtgcc atttccactc gggctgagct ggacagggag
     5101 gattttgagc acgtgaagaa cagcacgtac acagccctaa tcatagctac agacaatggt
     5161 tctccagttg ctactggaac agggacactt ctgctgatcc tgtctgatgt gaatgacaac
     5221 gcccccatac cagaacctcg aactatattc ttctgtgaga ggaatccaaa gcctcaggtc
     5281 ataaacatca ttgatgcaga ccttcctccc aatacatctc ccttcacagc agaactaaca
     5341 cacggggcga gtgccaactg gaccattcag tacaacgacc caacccaaga atctatcatt
     5401 ttgaagccaa agatggcctt agaggtgggt gactacaaaa tcaatctcaa gctcatggat
     5461 aaccagaata aagaccaagt gaccacctta gaggtcagcg tgtgtgactg tgaaggggcc
     5521 gctggcgtct gtaggaaggc acagcctgtc gaagcaggat tgcaaattcc tgccattctg
     5581 gggattcttg gaggaattct tgctttgcta attctgattc tgctgctctt gctgtttctt
     5641 cggaggagag cggtggtcaa agagccctta ctgcccccag aggatgacac ccgggacaac
     5701 gtttattact atgatgaaga aggaggcgga gaagaggacc aggactttga cttgagccag
     5761 ctgcacaggg gcctggacgc tcggcctgaa gtgactcgta acgacgttgc accaaccctc
     5821 atgagtgtcc cccggtatct tccccgccct gccaatcccg atgaaattgg aaattttatt
     5881 gatgaaaatc tgaaagcggc tgatactgac cccacagccc cgccttatga ttctctgctc
     5941 gtgtttgact atgaaggaag cggttccgaa gctgctagtc tgagctccct gaactcctca
     6001 gagtcagaca aagaccagga ctatgactac ttgaacgaat ggggcaatcg cttcaagaag
     6061 ctggctgaca tgtacggagg cggcgaggac gactagaacc cagctttctt gtacaaagtg
     6121 gttgatatcc agcacagtgg cggccgctcg agtctagagg gcccgcggtt cgaaggtaag
     6181 cctatcccta accctctcct cggtctcgat tctacgcgta ccggttagta atgagatccc
     6241 tccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc
     6301 tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc
     6361 cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt
     6421 ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct
     6481 gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa
     6541 aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt
     6601 tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag
     6661 gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt
     6721 acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt
     6781 ttcctttgaa aaacacgatg ataatatggc cacacatatg gtgagcaagg gcgaggagct
     6841 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     6901 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     6961 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
     7021 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     7081 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     7141 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     7201 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     7261 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     7321 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     7381 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
     7441 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     7501 cgggatcact ctcggcatgg acgagctgta caagtaaatc gatagatcct aatcaacctc
     7561 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     7621 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     7681 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     7741 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     7801 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     7861 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     7921 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
     7981 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     8041 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     8101 ctcagacgag tcggatctcc ctttgggccg cctccccgcc tgagatcctt taagaccaat
     8161 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     8221 gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt
     8281 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     8341 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     8401 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg
     8461 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggcc
     8521 cgggttaatt aaggaaaggg ctagatcatt cttgaagacg aaagggcctc gtgatacgcc
     8581 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     8641 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     8701 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     8761 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     8821 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     8881 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     8941 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg
     9001 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     9061 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     9121 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     9181 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     9241 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     9301 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     9361 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     9421 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     9481 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     9541 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     9601 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     9661 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     9721 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     9781 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     9841 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     9901 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     9961 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
    10021 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
    10081 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
    10141 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
    10201 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
    10261 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
    10321 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
    10381 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
    10441 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
    10501 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
    10561 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agcaagctca
    10621 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
    10681 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tccccgtggc
    10741 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
    10801 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
    10861 ttgtgagcgg ataacaattt cacacaggaa acagctatga catgattacg aatttcacaa
    10921 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
    10981 atcatgtctg gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc
    11041 cataccctat taccactgcc aattacctgt ggtttcattt actctaaacc tgtgattcct
    11101 ctgaattatt ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag
    11161 t
//
