LOCUS       sequence-240277-         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses aa324 to C-terminus of TP901 integrase with N-term FKBP 
            and C-term NLS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Weinberg BH, Cho JH, Agarwal Y, Pham NTH, Caraballo LD, Walkosz M, 
            Ortega C, Trexler M, Tague N, Law B, Benman WKJ, Letendre J, Beal J,
            Wong WW
  TITLE     High-performance chemical- and light-inducible recombinases in 
            mammalian cells and mice.
  JOURNAL   Nat Commun. 2019 Oct 24;10(1):4845. doi: 10.1038/s41467-019-12800-7.
  PUBMED    31649244
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-10-24"; volume: "10(1):4845. doi"; pages: " 
            10.1038/s41467-019-12800-7"
COMMENT     Sequence Label: pSD32_pCAG-PV1-FKBP-L1-TP901-324C-NLS-BGHpA
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    215..439
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(449..469)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cattggccac accagccacc accttctgat aggcagcctg cacctgagga gtgcgctagc
       61 ggtggaaggg ctcggagttg tggtaatcta tgtatcctgg gcggccgcgc ttggattctg
      121 cgtttgtttc cgtctacgaa ctcccagcgg tacacgctac agggcgcgtg gggatacccc
      181 ctagagcccc agctggttct ttccgcctca gaagccatag agcccaccgc atccccagca
      241 tgcctgctat tgtcttccca atcctccccc ttgctgtcct gccccacccc accccccaga
      301 atagaatgac acctactcag acaatgcgat gcaatttcct cattttatta ggaaaggaca
      361 gtgggagtgg caccttccag ggtcaaggaa ggcacggggg aggggcaaac aacagatggc
      421 tggcaactag aaggcacagg aattctcaca ccttccgctt tttcttgggg gccagctgga
      481 acttgaagat gatgtccacg ttgtcggcgg tcacgtccac cttagacacc aggttgttca
      541 caatcttttt cttgttatcg tagctcagct cattgatggg gatggagccc agctgtgttt
      601 tcacgagctc gaacacgtcg gtgctatcgt tgaacttgtt ctcagagatc ttggccttca
      661 gcagcttctt ctcggcctgc agagagtcgg tccggtcctt cagctcgtcc atggtgatga
      721 aatcgttcag gtacaggtcg ctgttcttct ggatcttctt gtcgatctgg ctgatctgct
      781 tcttgaagct ggaggtgtcc aggatgggct ggttgttgcc gttgatgatc ttcagcaggc
//
