LOCUS       sequence-240280-       10101 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway compatible N2H assay destination vector with C-terminus 
            tagging of nanoluc fragment1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10101)
  AUTHORS   Choi SG, Olivet J, Cassonnet P, Vidalain PO, Luck K, Lambourne L, 
            Spirohn K, Lemmens I, Dos Santos M, Demeret C, Jones L, Rangarajan 
            S, Bian W, Coutant EP, Janin YL, van der Werf S, Trepte P, Wanker 
            EE, De Las Rivas J, Tavernier J, Twizere JC, Hao T, Hill DE, Vidal 
            M, Calderwood MA, Jacob Y
  TITLE     Maximizing binary interactome mapping with a minimal number of 
            assays.
  JOURNAL   Nat Commun. 2019 Aug 29;10(1):3907. doi: 10.1038/s41467-019-11809-2.
  PUBMED    31467278
REFERENCE   2  (bases 1 to 10101)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-08-29"; volume: "10(1):3907. doi"; pages: " 
            10.1038/s41467-019-11809-2"
COMMENT     Sequence Label: pDEST-N2H-C1
FEATURES             Location/Qualifiers
     source          1..10101
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             complement(664..1758)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKVDAVLLGAVAGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANLLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(1771..2175)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     rep_origin      complement(2475..2930)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2612..2633)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2824..2843
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     enhancer        3067..3446
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     3823..3842
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3823..3841
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    4044..4168
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(4193..4198,4199..4216,4217..4223)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             4277..4936
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(4343..4362)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             5278..5583
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5462..5481
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5624..5748)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(6021..6054)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    6109..6230
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6120..6139)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6174..6193
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      6295..6482
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(6350..6374)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     protein_bind    complement(6487..6520)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     rep_origin      6535..6670
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6769..6786)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6940..7528)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7020..7039)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7699..8490,8491..8559))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8322..8341
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8560..8664)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      8691..10033
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(9654..9701)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     10074..10092
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatcga ctacgtcgtt aaggccgttt ctgacagagt aaaattcttg agggaacttt
      241 caccattatg ggaaatggtt caagaaggta ttgacttaaa ctccatcaaa tggtcaggtc
      301 attgagtgtt ttttatttgt tgtatttttt tttttttaga gaaaatcctc caatatataa
      361 attaggaatc atagtttcat gattttctgt tacacctaac tttttgtgtg gtgccctcct
      421 ccttgtcaat attaatgtta aagtgcaatt ctttttcctt atcacgttga gccattagta
      481 tcaatttgct tacctgtatt cctttacatc ctcctttttc tccttcttga taaatgtatg
      541 tagattgcgt atatagtttc gtctacccta tgaacatatt ccattttgta atttcgtgtc
      601 gtttctatta tgaatttcat ttataaagtt tatgtacaaa tatcataaaa aaagagaatc
      661 tttttaagca aggattttct taacttcttc ggcgacagca tcaccgactt cggtggtact
      721 gttggaacca cctaaatcac cagttctgat acctgcatcc aaaacctttt taactgcatc
      781 ttcaatggcc ttaccttctt caggcaagtt caatgacaat ttcaacatca ttgcagcaga
      841 caagatagtg gcgatagggt caaccttatt ctttggcaaa tctggagcag aaccgtggca
      901 tggttcgtac aaaccaaatg cggtgttctt gtctggcaaa gaggccaagg acgcagatgg
      961 caacaaaccc aaggaacctg ggataacgga ggcttcatcg gagatgatat caccaaacat
     1021 gttgctggtg attataatac catttaggtg ggttgggttc ttaactagga tcatggcggc
     1081 agaatcaatc aattgatgtt gaaccttcaa tgtagggaat tcgttcttga tggtttcctc
     1141 cacagttttt ctccataatc ttgaagaggc caaaagatta gctttatcca aggaccaaat
     1201 aggcaatggt ggctcatgtt gtagggccat gaaagcggcc attcttgtga ttctttgcac
     1261 ttctggaacg gtgtattgtt cactatccca agcgacacca tcaccatcgt cttcctttct
     1321 cttaccaaag taaatacctc ccactaattc tctgacaaca acgaagtcag tacctttagc
     1381 aaattgtggc ttgattggag ataagtctaa aagagagtcg gatgcaaagt tacatggtct
     1441 taagttggcg tacaattgaa gttctttacg gatttttagt aaaccttgtt caggtctaac
     1501 actaccggta ccccatttag gaccagccac agcacctaac aaaacggcat caaccttctt
     1561 ggaggcttcc agcgcctcat ctggaagtgg gacacctgta gcatcgatag cagcaccacc
     1621 aattaaatga ttttcgaaat cgaacttgac attggaacga acatcagaaa tagctttaag
     1681 aaccttaatg gcttcggctg tgatttcttg accaacgtgg tcacctggca aaacgacgat
     1741 cttcttaggg gcagacatag gggcagacat tagaatggta tatccttgaa atatatatat
     1801 atattgctga aatgtaaaag gtaagaaaag ttagaaagta agacgattgc taaccaccta
     1861 ttggaaaaaa caataggtcc ttaaataata ttgtcaactt caagtattgt gatgcaagca
     1921 tttagtcatg aacgcttctc tattctatat gaaaagccgg ttccggcctc tcacctttcc
     1981 tttttctccc aatttttcag ttgaaaaagg tatatgcgtc aggcgacctc tgaaattaac
     2041 aaaaaatttc cagtcatcga atttgattct gtgcgatagc gcccctgtgt gttctcgtta
     2101 tgttgaggaa aaaaataatg gttgctaaga gattcgaact cttgcatctt acgatacctg
     2161 agtattccca cagttaactg cggtcaagat atttcttgaa tcaggcgcct tagaccgctc
     2221 ggccaaacaa ccaattactt gttgagaaat agagtataat tatcctataa atataacgtt
     2281 tttgaacaca catgaacaag gaagtacagg acaattgatt ttgaagagaa tgtggatttt
     2341 gatgtaattg ttgggattcc atttttaata aggcaataat attaggtatg tggatatact
     2401 agaagttctc ctcgaccgtc gatatgcggt gtgaaatacc gcacagatgc gtaaggagaa
     2461 aataccgcat caggaaattg taaacgttaa tattttgtta aaattcgcgt taaatttttg
     2521 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
     2581 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
     2641 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
     2701 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
     2761 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
     2821 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
     2881 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt caatattggc
     2941 cattagccat attattcatt ggttatatag cataaatcaa tattggctat tggccattgc
     3001 atacgttgta tctatatcat aatatgtaca tttatattgg ctcatgtcca atatgaccgc
     3061 catgttggca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
     3121 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
     3181 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
     3241 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
     3301 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
     3361 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
     3421 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
     3481 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
     3541 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactgc gatcgggcgc
     3601 gcccctcctt gaattaatta aggcgcgcca ctgaaatttt taattaaggc gcgcctagca
     3661 tgtgattaat taagcatgtc tttagatcta tgatgacaca aaccccgccc agcgtcttgt
     3721 cattggcgaa ttcgaacacg cagatgcagt cggggcggcg cggtcccagg tccacttcgc
     3781 atataaaagg tgacgcgtgt ggatcctcga acaccgagct cttaatacga ctcactatag
     3841 ggaaagcaaa aatgtgatct tgcttgtaaa tacaattttg agaggttaat aaattacaag
     3901 tagtgctatt tttgtattta ggttagctat ttagctttac gttccaggat gcctagtggc
     3961 agccccacaa tatccaggaa gccctctctg cggtttttca gattaggtag tcgaaaaacc
     4021 taagaaattt acctatgccc atcacaagtt tgtacaaaaa agctgaacga gaaacgtaaa
     4081 atgatataaa tatcaatata ttaaattaga ttttgcataa aaaacagact acataatact
     4141 gtaaaacaca acatatccag tcactatggc ggccgcatta ggcaccccag gctttacact
     4201 ttatgcttcc ggctcgtata atgtgtggat tttgagttag gatccgtcga gattttcagg
     4261 agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca
     4321 atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca
     4381 gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt
     4441 ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg aattccgtat
     4501 ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt
     4561 ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca
     4621 gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc
     4681 taaagggttt attgagaata tgtttttcgt ctcagccaat ccctgggtga gtttcaccag
     4741 ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgggcaa
     4801 atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt
     4861 ttgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtact gcgatgagtg
     4921 gcagggcggg gcgtaaacgc gtggatccgg cttactaaaa gccagataac agtatgcgta
     4981 tttgcgcgct gatttttgcg gtataagaat atatactgat atgtataccc gaagtatgtc
     5041 aaaaagaggt atgctatgaa gcagcgtatt acagtgacag ttgacagcga cagctatcag
     5101 ttgctcaagg catatatgat gtcaatatct ccggtctggt aagcacaacc atgcagaatg
     5161 aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa tcaggaaggg atggctgagg
     5221 tcgcccggtt tattgaaatg aacggctctt ttgctgacga gaacaggggc tggtgaaatg
     5281 cagtttaagg tttacaccta taaaagagag agccgttatc gtctgtttgt ggatgtacag
     5341 agtgatatta ttgacacgcc cgggcgacgg atggtgatcc ccctggccag tgcacgtctg
     5401 ctgtcagata aagtctcccg tgaactttac ccggtggtgc atatcgggga tgaaagctgg
     5461 cgcatgatga ccaccgatat ggccagtgtg ccggtctccg ttatcgggga agaagtggct
     5521 gatctcagcc accgcgaaaa tgacatcaaa aacgccatta acctgatgtt ctggggaata
     5581 taaatgtcag gctcccttat acacagccag tctgcaggtc gaccatagtg actggatatg
     5641 ttgtgtttta cagtattatg tagtctgttt tttatgcaaa atctaattta atatattgat
     5701 atttatatca ttttacgttt ctcgttcagc tttcttgtac aaagtggtga tgggtggagg
     5761 gggcagtggt ggagggggca gtgtcttcac actcgaagat ttcgttgggg actggcgaca
     5821 gacagccggc tacaacctgg accaagtcct tgaacaggga ggtgtgtcca gtttgtttca
     5881 gaatctcggg gtgtccgtaa ctccgatcca aaggattgtc ctgagcggtg aaaatgggct
     5941 gaagatcgac atccatgtca tcatcccgta tgaatagtag tagtagtgat aataactata
     6001 acggtcctaa ggtagcgaac ataacttcgt atagcataca ttatacgaag ttatggaaaa
     6061 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaagaga agacatgata agatacattg
     6121 atgagtttgg acaaaccaca acaagaatgc agtgaaaaaa atgctttatt tgtgaaattt
     6181 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca
     6241 attgcattca ttttatgttt caggttcagg gggagatgtg ggaggttttt taaagcgaat
     6301 ttcttatgat ttatgatttt tattattaaa taagttataa aaaaaataag tgtatacaaa
     6361 ttttaaagtg actcttaggt tttaaaacga aaattcttgt tcttgagtaa ctctttcctg
     6421 taggtcaggt tgctttctca ggtatagcat gaggtcgctc ttattgacca cacctctacc
     6481 ggcggaataa cttcgtatag tataccttat acgaagttat gcctaactcc gcccatcccg
     6541 cccctaactc cgcccagttc cgcccattct ccgcctcatg gctgactaat tttttttatt
     6601 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggacgcttt
     6661 tttggaggcc gaggcttttg caaagatcga acaagagaca ggactgcatt aatgaatcgg
     6721 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     6781 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     6841 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     6901 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     6961 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     7021 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     7081 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     7141 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     7201 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     7261 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     7321 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     7381 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     7441 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     7501 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     7561 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     7621 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     7681 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     7741 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     7801 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     7861 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     7921 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     7981 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     8041 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     8101 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     8161 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     8221 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     8281 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     8341 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     8401 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     8461 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     8521 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     8581 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     8641 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg aacgaagcat
     8701 ctgtgcttca ttttgtagaa caaaaatgca acgcgagagc gctaattttt caaacaaaga
     8761 atctgagctg catttttaca gaacagaaat gcaacgcgaa agcgctattt taccaacgaa
     8821 gaatctgtgc ttcatttttg taaaacaaaa atgcaacgcg agagcgctaa tttttcaaac
     8881 aaagaatctg agctgcattt ttacagaaca gaaatgcaac gcgagagcgc tattttacca
     8941 acaaagaatc tatacttctt ttttgttcta caaaaatgca tcccgagagc gctatttttc
     9001 taacaaagca tcttagatta ctttttttct cctttgtgcg ctctataatg cagtctcttg
     9061 ataacttttt gcactgtagg tccgttaagg ttagaagaag gctactttgg tgtctatttt
     9121 ctcttccata aaaaaagcct gactccactt cccgcgttta ctgattacta gcgaagctgc
     9181 gggtgcattt tttcaagata aaggcatccc cgattatatt ctataccgat gtggattgcg
     9241 catactttgt gaacagaaag tgatagcgtt gatgattctt cattggtcag aaaattatga
     9301 acggtttctt ctattttgtc tctatatact acgtatagga aatgtttaca ttttcgtatt
     9361 gttttcgatt cactctatga atagttctta ctacaatttt tttgtctaaa gagtaatact
     9421 agagataaac ataaaaaatg tagaggtcga gtttagatgc aagttcaagg agcgaaaggt
     9481 ggatgggtag gttatatagg gatatagcac agagatatat agcaaagaga tacttttgag
     9541 caatgtttgt ggaagcggta ttcgcaatat tttagtagct cgttacagtc cggtgcgttt
     9601 ttggtttttt gaaagtgcgt cttcagagcg cttttggttt tcaaaagcgc tctgaagttc
     9661 ctatactttc tagagaatag gaacttcgga ataggaactt caaagcgttt ccgaaaacga
     9721 gcgcttccga aaatgcaacg cgagctgcgc acatacagct cactgttcac gtcgcaccta
     9781 tatctgcgtg ttgcctgtat atatatatac atgagaagaa cggcatagtg cgtgtttatg
     9841 cttaaatgcg tacttatatg cgtctattta tgtaggatga aaggtagtct agtacctcct
     9901 gtgatattat cccattccat gcggggtatc gtatgcttcc ttcagcacta ccctttagct
     9961 gttctatatg ctgccactcc tcaattggat tagtctcatc cttcaatgct atcatttcct
    10021 ttgatattgg atcatactaa gaaaccatta ttatcatgac attaacctat aaaaataggc
    10081 gtatcacgag gccctttcgt c
//
