LOCUS       sequence-240317-       10745 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of EZH2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10745)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 10745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-EZH2
FEATURES             Location/Qualifiers
     source          1..10745
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5705..5729)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5737..5755
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5780..5799)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5781..5799)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(5805..5825)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             5879..5920
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5941..6508
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5958..6510
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6102..6119)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6329..6348
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6522..7121
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6522..6541)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7018..7038
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7135..7723
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7188..7208)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7606..7617)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7798..8031
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8150..8168)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8236..8340
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8341..8409,8410..9201)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8559..8578)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9372..9960
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9861..9880
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10114..10131
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10371..10392
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10407..10412,10413..10430,10431..10437)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10445..10461
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10450..10472
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10469..10485
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10469..10485
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10535..10554
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagc aggcaccatg ggccagactg ggaagaaatc tgagaaggga
     3481 ccagtttgtt ggcggaagcg tgtaaaatca gagtacatgc gactgagaca gctcaagagg
     3541 ttcagacgag ctgatgaagt aaagagtatg tttagttcca atcgtcagaa aattttggaa
     3601 agaacggaaa tcttaaacca agaatggaaa cagcgaagga tacagcctgt gcacatcctg
     3661 acttctgtga gctcattgcg cgggactagg gagtgttcgg tgaccagtga cttggatttt
     3721 ccaacacaag tcatcccatt aaagactctg aatgcagttg cttcagtacc cataatgtat
     3781 tcttggtctc ccctacagca gaattttatg gtggaagatg aaactgtttt acataacatt
     3841 ccttatatgg gagatgaagt tttagatcag gatggtactt tcattgaaga actaataaaa
     3901 aattatgatg ggaaagtaca cggggataga gaatgtgggt ttataaatga tgaaattttt
     3961 gtggagttgg tgaatgccct tggtcaatat aatgatgatg acgatgatga tgatggagac
     4021 gatcctgaag aaagagaaga aaagcagaaa gatctggagg atcaccgaga tgataaagaa
     4081 agccgcccac ctcggaaatt tccttctgat aaaatttttg aagccatttc ctcaatgttt
     4141 ccagataagg gcacagcaga agaactaaag gaaaaatata aagaactcac cgaacagcag
     4201 ctcccaggcg cacttcctcc tgaatgtacc cccaacatag atggaccaaa tgctaaatct
     4261 gttcagagag agcaaagctt acactccttt catacgcttt tctgtaggcg atgttttaaa
     4321 tatgactgct tcctacatcg taagtgcaat tattcttttc atgcaacacc caacacttat
     4381 aagcggaaga acacagaaac agctctagac aacaaacctt gtggaccaca gtgttaccag
     4441 catttggagg gagcaaagga gtttgctgct gctctcaccg ctgagcggat aaagacccca
     4501 ccaaaacgtc caggaggccg cagaagagga cggcttccca ataacagtag caggcccagc
     4561 acccccacca ttaatgtgct ggaatcaaag gatacagaca gtgataggga agcagggact
     4621 gaaacggggg gagagaacaa tgataaagaa gaagaagaga agaaagatga aacttcgagc
     4681 tcctctgaag caaattctcg gtgtcaaaca ccaataaaga tgaagccaaa tattgaacct
     4741 cctgagaatg tggagtggag tggtgcggaa gcctcaatgt ttagagtcct cattggcact
     4801 tactatgaca atttctgtgc cattgctagg ttaattggga ccaaaacatg tagacaggtg
     4861 tatgagttta gagtcaaaga atctagcatc atagctccag ctcccgctga ggatgtggat
     4921 actcctccaa ggaaaaagaa gaggaaacac cggttgtggg ctgcacactg cagaaagata
     4981 cagctgaaaa aggacggctc ctctaaccat gtttacaact atcaaccctg tgatcatcca
     5041 cggcagcctt gtgacagttc gtgcccttgt gtgatagcac aaaatttttg tgaaaagttt
     5101 tgtcaatgta gttcagagtg tcaaaaccgc tttccgggat gccgctgcaa agcacagtgc
     5161 aacaccaagc agtgcccgtg ctacctggct gtccgagagt gtgaccctga cctctgtctt
     5221 acttgtggag ccgctgacca ttgggacagt aaaaatgtgt cctgcaagaa ctgcagtatt
     5281 cagcggggct ccaaaaagca tctattgctg gcaccatctg acgtggcagg ctgggggatt
     5341 tttatcaaag atcctgtgca gaaaaatgaa ttcatctcag aatactgtgg agagattatt
     5401 tctcaagatg aagctgacag aagagggaaa gtgtatgata aatacatgtg cagctttctg
     5461 ttcaacttga acaatgattt tgtggtggat gcaacccgca agggtaacaa aattcgtttt
     5521 gcaaatcatt cggtaaatcc aaactgctat gcaaaagtta tgatggttaa cggtgatcac
     5581 aggataggta tttttgccaa gagagccatc cagactggcg aagagctgtt ttttgattac
     5641 agatacagcc aggctgatgc cctgaagtat gtcggcatcg aaagagaaat ggaaatccct
     5701 tagaacccag ctttcttgta caaagtggtg atatccaatt aaccctcact aaaggtgagt
     5761 aagtttgtat gtttaagtac cctatagtga gtcgtattac cacccaactt ttctatacaa
     5821 agtggttgat atccagcaca gtggcggccg ctcgagtcta gagggcccgc ggttcgaagg
     5881 taagcctatc cctaaccctc tcctcggtct cgattctacg cgtaccggtt agtaatgaga
     5941 tccctccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt
     6001 tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc
     6061 tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca
     6121 aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac
     6181 gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg
     6241 ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt
     6301 gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct
     6361 gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg
     6421 ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt
     6481 ggttttcctt tgaaaaacac gatgataata tggccacaca tatgaccgag tacaagccca
     6541 cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt
     6601 tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca
     6661 ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg
     6721 cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg
     6781 tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc
     6841 aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca
     6901 ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg
     6961 gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca
     7021 acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag
     7081 gaccgcgcac ctggtgcatg acccgcaagc ccggtgccta aatcgataga tcctaatcaa
     7141 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     7201 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     7261 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     7321 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     7381 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     7441 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     7501 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     7561 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     7621 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     7681 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctgagat cctttaagac
     7741 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     7801 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
     7861 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     7921 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     7981 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt
     8041 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
     8101 ggcccgggtt aattaaggaa agggctagat cattcttgaa gacgaaaggg cctcgtgata
     8161 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     8221 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     8281 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     8341 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     8401 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     8461 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     8521 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     8581 cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     8641 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     8701 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     8761 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     8821 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     8881 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     8941 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     9001 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     9061 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     9121 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     9181 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     9241 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     9301 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     9361 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     9421 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     9481 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     9541 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     9601 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     9661 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     9721 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     9781 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     9841 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     9901 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     9961 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
    10021 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
    10081 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
    10141 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagcaag
    10201 ctcatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
    10261 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctccccg
    10321 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
    10381 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
    10441 ggaattgtga gcggataaca atttcacaca ggaaacagct atgacatgat tacgaatttc
    10501 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
    10561 tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc
    10621 accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta aacctgtgat
    10681 tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta
    10741 gtagt
//
