LOCUS       sequence-240330-       12290 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination vector for C-terminal tagging with mCherry. The 
            expression is driven by the UASp promoter. Suitable for Drosophila 
            transformation and transfection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12290)
  AUTHORS   Vilmos P, Kristo I, Szikora S, Jankovics F, Lukacsovich T, Kari B, 
            Erdelyi M
  TITLE     The actin-binding ERM protein Moesin directly regulates spindle 
            assembly and function during mitosis.
  JOURNAL   Cell Biol Int. 2016 Jun;40(6):696-707. doi: 10.1002/cbin.10607. Epub
            2016 Apr 6.
  PUBMED    27006187
REFERENCE   2  (bases 1 to 12290)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/cbin.10607"; journalName: "Cell Biol Int"; date: "2016-06";
            volume: "40"; issue: "6"; pages: "696-707"
COMMENT     Sequence Label: pPWCh
FEATURES             Location/Qualifiers
     source          1..12290
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    join(1..31,32..586)
                     /label=P element 5' end
     CDS             complement(join(1365..1979,2050..2181,2385..2700,2762..3416,
                     3491..3764,4166..4237))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     4689..4707
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     protein_bind    5404..5528
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(5553..5558,5559..5576,5577..5583)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             5637..6317
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     primer_bind     complement(5703..5722)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             6637..6942
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     6821..6840
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(6983..7107)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      7115..7124
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7121..7831
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7266..7284)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7534..7553
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    join(9297..9498,9499..9529)
                     /label=P element 3' end
                     /note="P element 3' end"
     primer_bind     complement(10067..10086)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10186..10208
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10246..10264)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10332..10436
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10437..10505,10506..11297)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10655..10674)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11468..12056
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11957..11976
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgatgaaa taacataagg tggtcccgtc gatagccgaa gcttaccgaa gtatacactt
       61 aaattcagtg cacgtttgct tgttgagagg aaaggttgtg tgcggacgaa tttttttttg
      121 aaaacattaa cccttacgtg gaataaaaaa aaatgaaata ttgcaaattt tgctgcaaag
      181 ctgtgactgg agtaaaatta attcacgtgc cgaagtgtgc tattaagaga aaattgtggg
      241 agcagagcct tgggtgcagc cttggtgaaa actcccaaat ttgtgatacc cactttaatg
      301 attcgcagtg gaaggctgca cctgcaaaag gtcagacatt taaaaggagg cgactcaacg
      361 cagatgccgt acctagtaaa gtgatagagc ctgaaccaga aaagataaaa gaaggctata
      421 ccagtgggag tacacaaaca gagtaagttt gaatagtaaa aaaaatcatt tatgtaaaca
      481 ataacgtgac tgtgcgttag gtcctgttca ttgtttaatg aaaataagag cttgagggaa
      541 aaaattcgta ctttggagta cgaaatgcgt cgtttagagc agcagccgaa ttaattctag
      601 ttccagtgaa atccaagcat tttctaaatt aaatgtattc ttattattat agttgttatt
      661 tttgatatat ataaacaaca ctattatgcc caccattttt ttgagatgca tctacacaag
      721 gaacaaacac tggatgtcac tttcagttca aattgtaacg ctaatcactc cgaacaggtc
      781 acaaaaaatt accttaaaaa gtcataatat taaattagaa taaatatagc tgtgagggaa
      841 atatatacaa atatattgga gcaaataaat tgtacataca aatatttatt actaatttct
      901 attgagacga aatgaaccac tcggaaccat ttgagcgaac cgaatcgcgc ggaactaacg
      961 acagtcgctc caaggtcgtc gaacaaaagg tgaatgtgtt gcggagagcg ggtgggagac
     1021 agcgaaagag caactacgaa acgtggtgtg gtggaggtga attatgaaga gggcgcgcga
     1081 tttgaaaagt atgtatataa aaaatatatc ccggtgtttt atgtagcgat aaacgagttt
     1141 ttgatgtaag gtatgcaggt gtgtaagtct tttggttaga agacaaatcc aaagtctact
     1201 tgtggggatg ttcgaagggg aaatacttgt attctatagg tcatatcttg tttttattgg
     1261 cacaaatata attacattag ctttttgagg gggcaataaa cagtaaacac gatggtaata
     1321 atggtaaaaa aaaaaaacaa gcagttattt cggatatatg tcggctactc cttgcgtcgg
     1381 gcccgaagtc ttagagccag atatgcgagc acccggaagc tcacgatgag aatggccaga
     1441 cccacgtagt ccagcggcag atcggcggcg gagaagttaa gcgtctccag gatgaccttg
     1501 cccgaactgg ggcacgtggt gttcgacgat gtgcagctaa tttcgcccgg ctccacgtcc
     1561 gcccattggt taatcagcag accctcgttg gcgtaacgga accatgagag gtacgacaac
     1621 catttgaggt atactggcac cgagcccgag ttcaagaaga agccgccaaa gagcaggaat
     1681 ggtatgataa ccggcggacc cacagacagc gccatcgagg tcgaggagct ggcgcaggat
     1741 attagatatc cgaaggacgt tgacacattg gccaccagag tgaccagcgc caggcagttg
     1801 aagaagtgca gcactccggc ccgcagtccg atcatcggat aggcaatcgc cgtgaagacc
     1861 agtggcactg tgagaaaaag cggcaattcg gcaatcgttt tgcccagaaa gtatgtgtca
     1921 cagcgataaa gtcgacttcg ggcctccctc ataaaaactg gcagctctga ggtgaacacc
     1981 taaatcgaat cgattcatta gaaagttagt aaattattga aatgcaaatg tattctaaac
     2041 atgacttaca tttatcgtgg caaagacgtt ttgaaaggtc atgttggtca ggaagaggaa
     2101 gatggctccg ttgatattca tcacacccac ttgcgtgagt tgttggccca aaaagatgag
     2161 gccaatcaag atggcaacca tctgcaaatt aaaatgttac tcgcatctca ttaatattcg
     2221 cgagttaaat gaaatttatt tatcttctgc aaaactataa actatacatc tcattgaaaa
     2281 aaactaagaa gggtgtggaa tcaggcaatt ctatctaaaa tctagcgaat ttgtttccaa
     2341 gaattgtaag cgttatatca tttgtttcca ctggaaccac tcaccgttgt ctgaataagt
     2401 cgcactttta cgaggagtgg ttccttgagc accgacagcc aggatcgcca caggaccgcc
     2461 cggaactgca tgaaccaggt ggccttgtag gtgtacccat tctccggctg ctccagtggc
     2521 ttctccagat ttttggtggc caacaactgc tccatatccc gggctacttt gctaatggca
     2581 aaattgtcgc atatcttggc gatccgatca cgggactcga tctcccgtcc gggcacaacg
     2641 gccaacacct gtacgtaaaa gtccgccgga ttgtagttgg taggacactg ggcacccacg
     2701 ctggatagga gttgagatgt tatgtaatac tagataccct taataaacac atcgaactca
     2761 ctaggaaaag aagtcgacgg cttcgctggg agtgcccaag aaagctaccc tgccctcggc
     2821 catcagaagg atcttgtcaa agagctcaaa cagctcggaa gacggctgat gaatggtcag
     2881 gatgacggtc ttgcccttct gcgacagctt cttcagcacc tggacgacgc tgtgggcggt
     2941 aaaggagtcc agtccggagg tgggctcatc gcagatcaga agcggcggat cggttagagc
     3001 ctcggaggcg aatgccagac gcttcctttc tccgccggac agacctttca ccctgccggg
     3061 cacaccgatg atcgtgtgct gacatttgct gagcgaaagc tcctggatca cctgatccac
     3121 gcgggccact cgctgccgat aggtcagatg tcgtggcatc cgcaccatgg cttggaaaat
     3181 caggtgttcc ctggccgtta gggagccgat aaagaggtca tcctgctgga cataggcgca
     3241 cctggcctgc atctccttgg cgtccacagg ttggccattg agcagtcgca tcccggatgg
     3301 cgatacttgg atgccctgcg gcgatcgaaa ggcaagggca ttcagcaggg tcgtctttcc
     3361 ggcaccggaa ctgcccatca cggccaaaag ttcgcccgga taggccacgc cgcaaactga
     3421 gtttcaaatt ggtaattgga ccctttatta agatttcaca cagatcagcc gactgcgaat
     3481 agaaactcac cgttcttgag caaatgtttc ctgggcgccg gtatgtgtcg ctcgttgcag
     3541 aatagtccgc gtgtccggtt gaccagctgc cgccatccgg agcccggctg attgaccgcc
     3601 ccaaagatgt ccatattgtg ccaggcatag gtgaggttct cggctagttg gccgctccct
     3661 gaaccggagt cctccggcgg actgggtggc cggagcgtgc cgtagttttt ggcctgcccg
     3721 aagccctggt taatgcagct ctgcgaagcc gctccgctgt caccctgcaa tgatagggga
     3781 tctcaaatat caactacaag cgttatgctc atctaacccc gaacaaaaag taccccgaag
     3841 tatcctacga agtaggttta tacttttatt tattttttgt gcatctagga tcagcttaaa
     3901 atatctggtt gttatatttt ttgtaaaaaa gaatatagtc gaaaatgaat gcctttagat
     3961 gtcttgatca tgatatgatc tcaaaaattg tcttatatag cgagaacagc taccagaata
     4021 atctgtttcg tgtcactatt tgtttgtgca attgcggttt gggatttttg tgggtcgcag
     4081 ttctcacgcc gcagacaatt tgatgttgca atcgcagttc ctatagatca agtgaactta
     4141 agatgtatgc acatgtacta ctcacattgt tcagatgctc ggcagatggg tgtttgctgc
     4201 ctccgcgaat taatagctcc tgatcctctt ggcccattgc cgggattttt cacactttcc
     4261 cctgcttacc cacccaaaac caatcaccac cccaatcact caaaaaacaa acaaaaataa
     4321 gaagcgagag gagttttggc acagcacttt gtgtttaatt gatggcgtaa accgcttgga
     4381 gcttcgtcac gaaaccgctg acaaaatgca actgaaggcg gacattgacg ctacgtaacg
     4441 ctacaaacgg tggcgaaaga gatagcggac gcagcggcga aagagacggc gatatttctg
     4501 tggacagaga aggaggcaaa cagcgctgac tttgagtgga atgtcatttt gagtgagagg
     4561 taatcgaaag aacctggtac atcaaatacc cttggatcga agtaaattta aaactgatca
     4621 gataagttca atgatatcca gtgcagtaaa aaaaaaaaat gtttttttta tctactttcc
     4681 gcaaaaatgg gttttattaa cttacataca tactagaatt ggccgctcta gccccccctc
     4741 gaatgttctc tctcttctct tctctctctc tttctcgaat gttctctctc ttctcttctc
     4801 tctctctttc tcgaggtcat caagcttagg cctccaaggc ggagtactgt cctccgggct
     4861 ggcggagtac tgtcctccgg caaggcggag tactgtcctc cgggctggcg gagtactgtc
     4921 ctccggcaag gcggagtact gtcctccggg ctggcggagt actgtcctcc ggcaaggcgg
     4981 agtactgtcc tccgggctgg cggagtactg tcctccggca aggcggagta ctgtcctccg
     5041 ggctggcgga gtactgtcct ccggcaaggc ggagtactgt cctccgggct ggcggagtac
     5101 tgtcctccgg caaggcggag tactgtcctc cgggctggcg gagtactgtc ctccggcaag
     5161 ggtcgagtcg atagccgaag cttaccgaag tatacactta aattcagtgc acgtttgctt
     5221 gttgagagga aaggttgtgt gcggacgaat ttttttttga aaaccggtga tagagcctga
     5281 accagaaaag ataaaagaag gctataccag tgggagtaca caaacagagt aagtttgaat
     5341 agtaaaaaaa atcatttatg taaacaataa cgtgactgtg cgttaggtcc tgttcattga
     5401 tcgacaagtt tgtacaaaaa agctgaacga gaaacgtaaa atgatataaa tatcaatata
     5461 ttaaattaga ttttgcataa aaaacagact acataatact gtaaaacaca acatatccag
     5521 tcactatggc ggccgcatta ggcaccccag gctttacact ttatgcttcc ggctcgtata
     5581 atgtgtggat tttgagttag gatccgtcga gattttcagg agctaaggaa gctaaaatgg
     5641 agaaaaaaat cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt
     5701 ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag ctggatatta
     5761 cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggcc tttattcaca
     5821 ttcttgcccg cctgatgaat gctcatccgg aattccgtat ggcaatgaaa gacggtgagc
     5881 tggtgatatg ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa actgaaacgt
     5941 tttcatcgct ctggagtgaa taccacgacg atttccggca gtttctacac atatattcgc
     6001 aagatgtggc gtgttacggt gaaaacctgg cctatttccc taaagggttt attgagaata
     6061 tgtttttcgt ctcagccaat ccctgggtga gtttcaccag ttttgattta aacgtggcca
     6121 atatggacaa cttcttcgcc cccgttttca ccatgggcaa atattatacg caaggcgaca
     6181 aggtgctgat gccgctggcg attcaggttc atcatgccgt ttgtgatggc ttccatgtcg
     6241 gcagaatgct taatgaatta caacagtact gcgatgagtg gcaggcgggg cgtaatctag
     6301 aggatccggc ttactaaaag ccagataaca gtatgcgtat ttgcgcgctg atttttgcgg
     6361 tataagaata tatactgata tgtatacccg aagtatgtca aaaagaggta tgctatgaag
     6421 cagcgtatta cagtgacagt tgacaccgac agctatcagt tgctcaaggc atatatgatg
     6481 tcaatatctc cggtctggta agcacaacca tgcagaatga agcccgtcgt ctgcgtgccg
     6541 aacgctggaa agcggaaaat caggaaggga tggctgaggt cgcccggttt attgaaatga
     6601 acggctcttt tgctgacgag aacaggggct ggtgaaatgc agtttaaggt ttacacctat
     6661 aaaagagaga gccgttatcg tctgtttgtg gatgtacaga gtgatattat tgacacgccc
     6721 gggcgacgga tggtgatccc cctggccagt gcacgtctgc tgtcagataa agtcccccgt
     6781 gaactttacc cggtggtgca tatcggggat gaaagctggc gcatgatgac caccgatatg
     6841 gccagtgtgc cggtctccgt tatcggggaa gaagtggctg atctcagcca ccgcgaaaat
     6901 gacatcaaaa acgccattaa cctgatgttc tggggaatat aaatgtcagg ctcccttata
     6961 cacagccagt ctgcaggtcg accatagtga ctggatatgt tgtgttttac agtattatgt
     7021 agtctgtttt ttatgcaaaa tctaatttaa tatattgata tttatatcat tttacgtttc
     7081 tcgttcagct ttcttgtaca aagtggtgag ctccgccacc atggtgagca agggcgagga
     7141 ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt
     7201 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca
     7261 gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc
     7321 ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta
     7381 cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg
     7441 cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt
     7501 gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga agacgatggg
     7561 ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa
     7621 gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa
     7681 ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt tggacatcac
     7741 ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc
     7801 caccggcggc atggacgagc tgtacaagta agaattcgtt aactcgagga gcttgataac
     7861 attataccnt atacaagtaa gaattcgtta actcgaggag cttgataaca ttatacctaa
     7921 acccatggtc aagagtaaac atttctgcct ttgaagttga gaacacaatt aagcatcccc
     7981 tggttaaacc tgacattcat acttgttaat agcgccataa acatagcacc aatttcgaag
     8041 aaatcagtta aaagcaatta gcaattagca attagcaata actctgctga cttcaaaacg
     8101 agaagagttg caagtatttg taaggcacag tttatagacc accgacggct cattagggct
     8161 cgtcatgtaa ctaagcgcgg tgaaacccaa ttgaacatat agtggaatta ttattatcaa
     8221 tggggaagat ttaaccctca ggtagcaaag taatttaatt gcaaatagag agtcctaaga
     8281 ctaaataata tatttaaaaa tctggccctt tgaccttgct tgtcaggtgc atttgggttc
     8341 aatcgtaagt tgcttctata taaacacttt ccccatcccc gcaataatga agaataccgc
     8401 agaataaaga gagatttgca acaaaaaata aaggcattgc gaaaactttt tatgggggat
     8461 cattacactc gggcctacgg ttacaattcc cagccactta agcgacaagt ttggccaaca
     8521 atccatctaa tagctaatag cgcaatcact ggtaatcgca agagtatata ggcaatagaa
     8581 cccatggatt tgaccaaagg taaccgagac aatggagaag caagaggatt tcaaactgaa
     8641 cacccacagt gctgtgtact accactggcg cgtttgggag ctcactggcc tgatgcgtcc
     8701 tccgggcgtt tcaagcctgc tttacgtggt atactccatt acggtcaact tggtggtcac
     8761 cgtgctgttt cccttgagct tgctggccag gctgctgttc accaccaaca tggccggatt
     8821 gtgcgagaac ctgaccataa ctattaccga tattgtggcc aatttgaagt ttgcgaatgt
     8881 gtacatggtg aggaagcagc tccatgagat tcgctctctc ctaaggctca tggacgctag
     8941 agcccggctg gtgggcgatc ccgaggagat ttctgccttg aggaaggaag tgaatatcgc
     9001 acagggcact ttccgcacct ttgccagtat tttcgtattt ggcactactt tgagttgcgt
     9061 ccgcgtggtc gttcgcccgg atcgagagct cctgtatccg gcctggttcg gcgttgactg
     9121 gatgcactcc accagaaact atgtgctcat caatatctac cagctcttcg gcttgatagt
     9181 gcaggctata cagaactgcg ctagtgactc ctatccgcct gcgtttctct gcctgctcac
     9241 gggtcatatg cgtgctttgg agctgagggt gcggcggatt ggctgcagcc aagctttgcg
     9301 tactcgcaaa ttattaaaaa taaaacttta aaaataattt cgtctaatta atattatgag
     9361 ttaattcaaa ccccacggac atgctaaggg ttaatcaaca atcatatcgc tgtctcactc
     9421 agactcaata cgacactcag aatactattc ctttcactcg cacttattgc aagcatacgt
     9481 taagtggatg tctcttgccg acgggaccac cttatgttat ttcatcatgg tctggccatt
     9541 ctcatcgtga gcttccgggt gctcgcatat ctggctctaa gacttcgggc ccgacgcaag
     9601 gagtagccga catatatccg aaataactgc ttgttttttt tttttaccat tattaccatc
     9661 gtgtttactg tttattgccc cctcaaaaag ctaatgtaat tatatttgtg ccaataaaaa
     9721 caagatatga cctatagaat acaagtattt ccccttcgaa catccccaca agtagacttt
     9781 ggatttgtct tctaaccaaa agacttacac acctgcatac cttacatcaa aaactcgttt
     9841 atcgctacat aaaacaccgg gatatatttt ttatatacat acttttcaaa tcgcgcgccc
     9901 tcttcataat tcacctccac cacaccacgt ttcgtagttg ctctttcgct gtctcccacc
     9961 cgctctccgc aacacattca ccttttgttc gacgaccttg gagcgactgt cgttagttcc
    10021 gcgcgattcg gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg
    10081 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
    10141 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
    10201 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
    10261 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
    10321 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
    10381 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
    10441 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
    10501 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
    10561 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
    10621 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
    10681 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
    10741 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
    10801 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
    10861 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
    10921 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
    10981 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
    11041 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
    11101 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
    11161 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
    11221 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
    11281 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
    11341 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
    11401 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
    11461 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
    11521 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
    11581 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
    11641 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
    11701 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
    11761 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
    11821 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
    11881 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
    11941 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
    12001 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
    12061 ccttcttctt gaactcgggc tcggtgccag tatacctcaa atggttgtcg tacctctcat
    12121 ggttccgtta cgccaacgag ggtctgctga ttaaccaatg ggcggacgtg gagccgggcg
    12181 aaattagctg cacatcgtcg aacaccacgt gccccagttc gggcaaggtc atcctggaga
    12241 cgcttaactt ctccgccgcc gatctgccgc tggactacgt gggtctggcc
//
