LOCUS       sequence-24034-e         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  TITLE     T4 Lysozyme Plasmids - Tray 1
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: T4 Lysozyme E22K WT*
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(707..729)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    718..734
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 gaaaagcccg ctcattaggc gggctcagat ctgctcatgt ttgacagctt atcatcgata
       61 agcttcggca ataaattctt gatatgtttt catatgtttc ctttaaatat tttaattagt
      121 aattgtctat tcaagtaatt gtgaatatac tatcacaatt ccaagagaaa gtaaacagct
      181 ttatagattt ttatacgcgt cccaagtgcc agttctaaac gttgtaatga ctcgttttgc
      241 gcgattaggt gtttgattat accatctact tttagctaag ttaactgctg cttcatccca
      301 gcgtttttgt tgaagcatac gtaaagagtt agtaaatcct gccacaccgg tttctcccat
      361 ttggaaaacc atattaatca atgcagcgcg acgaaccgca tcaagagaat cataaaccgg
      421 ttttaattta gcatttctca gaattccgcg aacagcagca tcaacatcct gattaaagag
      481 tttttcagcc tcatcttttg taattacacc attagtatta cgcccaatag ctttatctaa
      541 ttcagattta gcagcattaa gtgatggact ttttgtaagc aaatgaccga tgccaatagt
      601 gtaatagcct tttgtgtctt tatagatttt aagtctaaga ccttcatcta tacgtaacat
      661 ttcaaatata ttcataatac ctcctaagca tcgatggatc ctgtttcctg tgtgaaattg
      721 ttatccgctc acaattccac acattatacg agccgatgat aatgtcacag gggatgggga
      781 gtagctganc tgtccnggng aatgtancgn ccatcaccat aanagcgaga tatgcacngg
      841 gagg
//
