LOCUS       sequence-240342-        9809 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expressing GFP-Atg13[3SA].
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9809)
  AUTHORS   Chew LH, Lu S, Liu X, Li FK, Yu AY, Klionsky DJ, Dong MQ, Yip CK
  TITLE     Molecular interactions of the Saccharomyces cerevisiae Atg1 complex 
            provide insights into assembly and regulatory mechanisms.
  JOURNAL   Autophagy. 2015;11(6):891-905. doi: 10.1080/15548627.2015.1040972.
  PUBMED    25998554
REFERENCE   2  (bases 1 to 9809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Autophagy."; date: "2015"; volume: "11(6):891-905. doi"; pages: " 
            10.1080/15548627.2015.1040972"
COMMENT     Sequence Label: pGFP-ATG13[3SA](315)
FEATURES             Location/Qualifiers
     source          1..9809
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    79..100
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(115..120,121..138,139..145)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    153..169
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     158..180
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     177..193
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     177..193
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        214..232
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     260..276
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     261..277
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             395..1108
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFAYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             395..1108
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFAYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(425..453)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1048..1068
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(4162..4181)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4163..4181)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4188..4205)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4188..4204)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4197..4219)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4345..4800
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4432..4451)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4642..4663
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5101..5508
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             5509..6603
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTYLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(7097..7116)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7216..7238
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7276..7294)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    7336..7839
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        7876..7980
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7981..8049,8050..8841)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8199..8218)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9012..9600
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9501..9520
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9754..9771
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
       61 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
      121 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
      181 aaacagctat gaccatgatt acgccaagct cgaaattaac cctcactaaa gggaacaaaa
      241 gctggtaccg ggccccccct cgaggtcgac ggtatcgata agcttgatat cgaattcata
      301 ggtaccaaat acattccact tacaccttcc aggctcaagt cttgaaaaga aagcagaaca
      361 tacagcccgg ttgaatagca tgagtctgcc tagaatgagt aaaggagaag aacttttcac
      421 tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aatgggcaca aattttctgt
      481 cagtggagag ggtgaaggtg atgcaacata cggaaaactt acccttaaat ttatttgcac
      541 tactggaaaa ctacctgttc catggccaac acttgtcact actttcgcgt atggtcttca
      601 atgctttgcg agatacccag atcatatgaa acagcatgac tttttcaaga gtgccatgcc
      661 cgaaggttat gtacaggaaa gaactatatt tttcaaagat gacgggaact acaagacacg
      721 tgctgaagtc aagtttgaag gtgataccct tgttaataga atcgagttaa aaggtattga
      781 ttttaaagaa gatggaaaca ttcttggaca caaattggaa tacaactata actcacacaa
      841 tgtatacatc atggcagaca aacaaaagaa tggaatcaaa gttaacttca aaattagaca
      901 caacattgaa gatggaagcg ttcaactagc agaccattat caacaaaata ctccaattgg
      961 cgatggccct gtccttttac cagacaacca ttacctgtcc acacaatctg ccctttcgaa
     1021 agatcccaac gaaaagagag accacatggt ccttcttgag tttgtaacag ctgctgggat
     1081 tacacatggc atggatgaac tatacaaatc gtctagggtt gccgaagagg acatcgagaa
     1141 gcaagtcctt caattgatag acagcttttt tctgaagact acactactaa tatgctccac
     1201 cgaatcaagt cgataccagt cttctacaga aaatatattc ctatttgacg acacatggtt
     1261 tgaagatcac tcagaattag tgagtgagct acccgagata atatcaaaat ggtctcacta
     1321 cgatggtcga aaagagttgc cacccttagt ggtagagaca tatttggatt taagacagtt
     1381 aaactcgtct catttagtta gattaaagga ccacgaaggc catttgtgga acgtttgcaa
     1441 aggaactaag aagcaggaaa tcgtgatgga acgttggctt atcgaattag ataattcatc
     1501 cccaactttc aaatcataca gtgaagatga gactgatgtt aatgaacttt ctaaacagct
     1561 agtccttctc ttccgttatt tgttgacttt aatacagtta ctacccacaa cagaattata
     1621 ccaattatta ataaagtctt ataacggccc gcaaaatgaa ggaagttcca atccaataac
     1681 ttccacgggc ccactagtaa gtatccggac gtgtgtcctt gacggatcta aaccaatttt
     1741 atcgaagggg agaatagggt tgagcaaacc gattattaat acatattcca atgcgcttaa
     1801 cgaatcaaac ctgccagccc atttagatca aaagaagatc acacctgtat ggacaaagtt
     1861 tggactctta agagtctcgg tatcatacag acgtgattgg aagtttgaaa ttaacaatac
     1921 aaacgacgaa ttattttcag ctcgacatgc atctgtctca cataactcac aaggacccca
     1981 gaatcagcca gaacaagaag gacaaagtga tcaagacata gggaaacgcc aaccacaatt
     2041 tcaacagcag cagcagcccc aacagcagca gcagcagcag caacagcaac agagacaaca
     2101 ccaggtccag acacaacaac aaagacagat acctgatagg agatctcttt cactttctcc
     2161 ttgtacaaga gccaattctt ttgaaccaca atcttggcag aagaaagtct atccaatatc
     2221 gagacctgtt caaccattta aagttggttc aattggaagt caaagtgcaa gcagaaatcc
     2281 ctctaattca tcgtttttca accaaccacc tgttcatagg ccaagtatga gctccaacta
     2341 cgggccacaa atgaatattg aaggtaccag tgttggaagc acctcaaagt attcctcctc
     2401 ctttgggaac attcgtcgtc actcaagtgt aaagacgaca gagaatgctg aaaaagtatc
     2461 aaaagctgta aagagcccac tacaacctca agaatcacaa gaagatttaa tggattttgt
     2521 taaattactc gaagaaaaac ccgatctaac tataaagaag acaagtggaa ataatccacc
     2581 caatatcaat atttctgatt ctctaatcag atatcagaat ttgaagccaa gtaatgactt
     2641 attaagtgaa gatttatccg taagtttatc catggatcca aatcatacat atcacagagg
     2701 cagatcagat tcccactcac cattgccttc aatatcccct tcgatgcatt atggatcgtt
     2761 gaactcgaga atgtctcaag gcgccaatgc aagccatttg attgcaagag gcggtgggaa
     2821 ttcatctact agtgccttga atagtagaag gaattcttta gataagagct caaacaagca
     2881 gggtatgtca ggcttacctc ctatttttgg tggagagagt acttcatatc accacgacaa
     2941 caaaatacaa aagtacaacc aattaggagt agaagaagat gatgatgacg agaatgaccg
     3001 tttgctcaac caaatgggaa acagtgctac aaaattcaaa agttcaatag caccaagagc
     3061 aattgatgcc atttcaagtt ctttcataaa aagtaggata cctatcagac aaccatacca
     3121 ttactctcaa ccaactactg cgccctttca agctcaggcg aaatttcata aacctgcaaa
     3181 taagttaatc gataatggta ataggagtaa tagtaacaat aacaatcata atgggaatga
     3241 tgcagttggt gtgatgcata atgacgagga tgatcaagat gatgatctag tatttttcat
     3301 gagtgatatg aacctttcta aagaaggtta aaaatggtaa agttttattt taaagggcac
     3361 aactaaagaa aaataatcaa aaaaaaggtg aaatagatat gcaagaaaag aaaaaaaaat
     3421 acaatactag taaactgaaa taaaaaagat tagccataat ggatttgttt ctctttgtta
     3481 tcgatattct tacctcttga agatccatgt gtctatcatt tgaattgaat atataattat
     3541 atacagtgag atataccaat tgcagtaaca aaatggcaaa tatgtatatc agtaatataa
     3601 ttagcatata aaataaaaaa aaaaaatgct tatttgtcac ccgctgactg cgtttccttt
     3661 tgaattataa aaggttcctt atggttctgg tccactagcg ttttattttc ttccttttcc
     3721 tgtaatattt ttaactttag ctcatgtact ttgtgcttgt ctatatgttt ctcgtatttt
     3781 tcctttgtct tgaaagtcct agaacaatta ttgtaaaagc atctgtattt tcttccaacg
     3841 gtattcaata aaaggtttag cccattttca ccagactcaa gtcgcttttc taaatccact
     3901 tcattctgta aaaactctac attattgttt tcggtaagtt ttctcctttt cctcaaccta
     3961 ttggaaatct tctcttcatc ctgctcattc gagtgtttgc tattccccag ttgaactccg
     4021 tggtcttgga caagttgttg aatatcagaa attttatatt ttagttccag tgggatatct
     4081 tcttccgtat gaatagaccc ataatgcgtg agaagatcat gttttctaga gcggccgcca
     4141 ccgcggtgga gctccaattc gccctatagt gagtcgtatt acaattcact ggccgtcgtt
     4201 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     4261 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     4321 ttgcgcagcc tgaatggcga atggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     4381 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     4441 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     4501 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     4561 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     4621 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     4681 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     4741 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     4801 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatcgaccgg
     4861 tcgaggagaa cttctagtat atctacatac ctaatattat tgccttatta aaaatggaat
     4921 cccaacaatt acatcaaaat ccacattctc ttcaaaatca attgtcctgt acttccttgt
     4981 tcatgtgtgt tcaaaaacgt tatatttata ggataattat actctatttc tcaacaagta
     5041 attggttgtt tggccgagcg gtctaaggcg cctgattcaa gaaatatctt gaccgcagtt
     5101 aactgtggga atactcaggt atcgtaagat gcaagagttc gaatctctta gcaaccatta
     5161 tttttttcct caacataacg agaacacaca ggggcgctat cgcacagaat caaattcgat
     5221 gactggaaat tttttgttaa tttcagaggt cgcctgacgc atataccttt ttcaactgaa
     5281 aaattgggag aaaaaggaaa ggtgagagcg ccggaaccgg cttttcatat agaatagaga
     5341 agcgttcatg actaaatgct tgcatcacaa tacttgaagt tgacaatatt atttaaggac
     5401 ctattgtttt ttccaatagg tggttagcaa tcgtcttact ttctaacttt tcttaccttt
     5461 tacatttcag caatatatat atatatattt caaggatata ccattctaat gtctgcccct
     5521 aagaagatcg tcgttttgcc aggtgaccac gttggtcaag aaatcacagc cgaagccatt
     5581 aaggttctta aagctatttc tgatgttcgt tccaatgtca agttcgattt cgaaaatcat
     5641 ttaattggtg gtgctgctat cgatgctaca ggtgttccac ttccagatga ggcgctggaa
     5701 gcctccaaga aggctgatgc cgttttgtta ggtgctgtgg gtggtcctaa atggggtacc
     5761 ggtagtgtta gacctgaaca aggtttacta aaaatccgta aagaacttca attgtacgcc
     5821 aacttaagac catgtaactt tgcatccgac tctcttttag acttatctcc aatcaagcca
     5881 caatttgcta aaggtactga cttcgttgtt gtcagagaat tagtgggagg tatttacttt
     5941 ggtaagagaa aggaagacga tggtgatggt gtcgcttggg atagtgaaca atacaccgtt
     6001 ccagaagtgc aaagaatcac aagaatggcc gctttcatgg ccctacaaca tgagccacca
     6061 ttgcctattt ggtccttgga taaagctaat gttttggcct cttcaagatt atggagaaaa
     6121 actgtggagg aaaccatcaa gaacgaattc cctacattga aggttcaaca tcaattgatt
     6181 gattctgccg ccatgatcct agttaagaac ccaacctacc taaatggtat tataatcacc
     6241 agcaacatgt ttggtgatat catctccgat gaagcctccg ttatcccagg ttccttgggt
     6301 ttgttgccat ctgcgtcctt ggcctctttg ccagacaaga acaccgcatt tggtttgtac
     6361 gaaccatgcc acggttctgc tccagatttg ccaaagaata aggtcaaccc tatcgccact
     6421 atcttgtctg ctgcaatgat gttgaaattg tcattgaact tgcctgaaga aggtaaggcc
     6481 attgaagatg cagttaaaaa ggttttggat gcaggtatca gaactggtga tttaggtggt
     6541 tccaacagta ccaccgaagt cggtgatgct gtcgccgaag aagttaagaa aatccttgct
     6601 taaaaagatt ctcttttttt atgatatttg tacataaact ttataaatga aattcataat
     6661 agaaacgaca cgaaattaca aaatggaata tgttcatagg gtagacgaaa ctatatacgc
     6721 aatctacata catttatcaa gaaggagaaa aaggaggatg taaaggaata caggtaagca
     6781 aattgatact aatggctcaa cgtgataagg aaaaagaatt gcactttaac attaatattg
     6841 acaaggagga gggcaccaca caaaaagtta ggtgtaacag aaaatcatga aactatgatt
     6901 cctaatttat atattggagg attttctcta aaaaaaaaaa aatacaacaa ataaaaaaca
     6961 ctcaatgacc tgaccatttg atggagttta agtcaatacc ttcttgaacc atttcccata
     7021 atggtgaaag ttccctcaag aattttactc tgtcagaaac ggccttaacg acgtagtcga
     7081 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc
     7141 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     7201 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     7261 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     7321 tggtttctta ggacggatcg cttgcctgta acttacacgc gcctcgtatc ttttaatgat
     7381 ggaataattt gggaatttac tctgtgttta tttattttta tgttttgtat ttggatttta
     7441 gaaagtaaat aaagaaggta gaagagttac ggaatgaaga aaaaaaaata aacaaaggtt
     7501 taaaaaattt caacaaaaag cgtactttac atatatattt attagacaag aaaagcagat
     7561 taaatagata tacattcgat taacgataag taaaatgtaa aatcacagga ttttcgtgtg
     7621 tggtcttcta cacagacaag atgaaacaat tcggcattaa tacctgagag caggaagagc
     7681 aagataaaag gtagtatttg ttggcgatcc ccctagagtc ttttacatct tcggaaaaca
     7741 aaaactattt tttctttaat ttcttttttt actttctatt tttaatttat atatttatat
     7801 taaaaaattt aaattataat tatttttata gcacgtgatg aaaaggaccc aggtggcact
     7861 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     7921 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     7981 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     8041 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     8101 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     8161 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     8221 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     8281 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     8341 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     8401 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     8461 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     8521 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     8581 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     8641 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     8701 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     8761 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     8821 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     8881 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     8941 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     9001 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     9061 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     9121 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     9181 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     9241 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     9301 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     9361 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     9421 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     9481 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     9541 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     9601 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     9661 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     9721 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     9781 gagcgcccaa tacgcaaacc gcctctccc
//
