LOCUS       sequence-240343-        9809 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expressing GFP-Atg13.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9809)
  AUTHORS   Chew LH, Lu S, Liu X, Li FK, Yu AY, Klionsky DJ, Dong MQ, Yip CK
  TITLE     Molecular interactions of the Saccharomyces cerevisiae Atg1 complex 
            provide insights into assembly and regulatory mechanisms.
  JOURNAL   Autophagy. 2015;11(6):891-905. doi: 10.1080/15548627.2015.1040972.
  PUBMED    25998554
REFERENCE   2  (bases 1 to 9809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Autophagy."; date: "2015"; volume: "11(6):891-905. doi"; pages: " 
            10.1080/15548627.2015.1040972"
COMMENT     Sequence Label: pGFP-ATG13(315)
FEATURES             Location/Qualifiers
     source          1..9809
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    115..136
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(151..156,157..174,175..181)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    189..205
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     194..216
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     213..229
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     213..229
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        250..268
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     296..312
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     297..313
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             431..1144
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFAYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             431..1144
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFAYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(461..489)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1084..1104
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(4198..4217)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4199..4217)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4224..4241)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4224..4240)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4233..4255)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4381..4836
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4468..4487)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4678..4699
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5137..5544
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             5545..6639
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(7133..7152)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7252..7274
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7312..7330)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    7372..7875
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        7912..8016
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8017..8085,8086..8877)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8235..8254)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9048..9636
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9537..9556
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9790..9807
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
       61 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
      121 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
      181 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
      241 caagctcgaa attaaccctc actaaaggga acaaaagctg gtaccgggcc ccccctcgag
      301 gtcgacggta tcgataagct tgatatcgaa ttcataggta ccaaatacat tccacttaca
      361 ccttccaggc tcaagtcttg aaaagaaagc agaacataca gcccggttga atagcatgag
      421 tctgcctaga atgagtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga
      481 attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc
      541 aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg
      601 gccaacactt gtcactactt tcgcgtatgg tcttcaatgc tttgcgagat acccagatca
      661 tatgaaacag catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac
      721 tatatttttc aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga
      781 tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct
      841 tggacacaaa ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca
      901 aaagaatgga atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca
      961 actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga
     1021 caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca
     1081 catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata
     1141 caaatcgtct agggttgccg aagaggacat cgagaagcaa gtccttcaat tgatagacag
     1201 cttttttctg aagactacac tactaatatg ctccaccgaa tcaagtcgat accagtcttc
     1261 tacagaaaat atattcctat ttgacgacac atggtttgaa gatcactcag aattagtgag
     1321 tgagctaccc gagataatat caaaatggtc tcactacgat ggtcgaaaag agttgccacc
     1381 cttagtggta gagacatatt tggatttaag acagttaaac tcgtctcatt tagttagatt
     1441 aaaggaccac gaaggccatt tgtggaacgt ttgcaaagga actaagaagc aggaaatcgt
     1501 gatggaacgt tggcttatcg aattagataa ttcatcccca actttcaaat catacagtga
     1561 agatgagact gatgttaatg aactttctaa acagctagtc cttctcttcc gttatttgtt
     1621 gactttaata cagttactac ccacaacaga attataccaa ttattaataa agtcttataa
     1681 cggcccgcaa aatgaaggaa gttccaatcc aataacttcc acgggcccac tagtaagtat
     1741 ccggacgtgt gtccttgacg gatctaaacc aattttatcg aaggggagaa tagggttgag
     1801 caaaccgatt attaatacat attccaatgc gcttaacgaa tcaaacctgc cagcccattt
     1861 agatcaaaag aagatcacac ctgtatggac aaagtttgga ctcttaagag tctcggtatc
     1921 atacagacgt gattggaagt ttgaaattaa caatacaaac gacgaattat tttcagctcg
     1981 acatgcatct gtctcacata actcacaagg accccagaat cagccagaac aagaaggaca
     2041 aagtgatcaa gacataggga aacgccaacc acaatttcaa cagcagcagc agccccaaca
     2101 gcagcagcag cagcagcaac agcaacagag acaacaccag gtccagacac aacaacaaag
     2161 acagatacct gataggagat ctctttcact ttctccttgt acaagagcca attcttttga
     2221 accacaatct tggcagaaga aagtctatcc aatatcgaga cctgttcaac catttaaagt
     2281 tggttcaatt ggaagtcaaa gtgcgagcag aaatccctct aattcatcgt ttttcaacca
     2341 accacctgtt cataggccaa gtatgagctc caactacggg ccacaaatga atattgaagg
     2401 taccagtgtt ggaagcacct caaagtattc ctcctccttt gggaacattc gtcgtcactc
     2461 aagtgtaaag acgacagaga atgctgaaaa agtatcaaaa gctgtaaaga gcccactaca
     2521 acctcaagaa tcacaagaag atttaatgga ttttgttaaa ttactcgaag aaaaacccga
     2581 tctaactata aagaagacaa gtggaaataa tccacccaat atcaatattt ctgattctct
     2641 aatcagatat cagaatttga agccaagtaa tgacttatta agtgaagatt tatccgtaag
     2701 tttatccatg gatccaaatc atacatatca cagaggcaga tcagattccc actcaccatt
     2761 gccttcaata tccccttcga tgcattatgg atcgttgaac tcgagaatgt ctcaaggcgc
     2821 caatgcaagc catttgattg caagaggcgg tgggaattca tctactagtg ccttgaatag
     2881 tagaaggaat tctttagata agagctcaaa caagcagggt atgtcaggct tacctcctat
     2941 ttttggtgga gagagtactt catatcacca cgacaacaaa atacaaaagt acaaccaatt
     3001 aggagtagaa gaagatgatg atgacgagaa tgaccgtttg ctcaaccaaa tgggaaacag
     3061 tgctacaaaa ttcaaaagtt caatatctcc aagatcaatt gatagcattt caagttcttt
     3121 cataaaaagt aggataccta tcagacaacc ataccattac tctcaaccaa ctactgcgcc
     3181 ctttcaagct caggcgaaat ttcataaacc tgcaaataag ttaatcgata atggtaatag
     3241 gagtaatagt aacaataaca atcataatgg gaatgatgca gttggtgtga tgcataatga
     3301 cgaggatgat caagatgatg atctagtatt tttcatgagt gatatgaacc tttctaaaga
     3361 aggttaaaaa tggtaaagtt ttattttaaa gggcacaact aaagaaaaat aatcaaaaaa
     3421 aaggtgaaat agatatgcaa gaaaagaaaa aaaaatacaa tactagtaaa ctgaaataaa
     3481 aaagattagc cataatggat ttgtttctct ttgttatcga tattcttacc tcttgaagat
     3541 ccatgtgtct atcatttgaa ttgaatatat aattatatac agtgagatat accaattgca
     3601 gtaacaaaat ggcaaatatg tatatcagta atataattag catataaaat aaaaaaaaaa
     3661 aatgcttatt tgtcacccgc tgactgcgtt tccttttgaa ttataaaagg ttccttatgg
     3721 ttctggtcca ctagcgtttt attttcttcc ttttcctgta atatttttaa ctttagctca
     3781 tgtactttgt gcttgtctat atgtttctcg tatttttcct ttgtcttgaa agtcctagaa
     3841 caattattgt aaaagcatct gtattttctt ccaacggtat tcaataaaag gtttagccca
     3901 ttttcaccag actcaagtcg cttttctaaa tccacttcat tctgtaaaaa ctctacatta
     3961 ttgttttcgg taagttttct ccttttcctc aacctattgg aaatcttctc ttcatcctgc
     4021 tcattcgagt gtttgctatt ccccagttga actccgtggt cttggacaag ttgttgaata
     4081 tcagaaattt tatattttag ttccagtggg atatcttctt ccgtatgaat agacccataa
     4141 tgcgtgagaa gatcatgttt tctagagcgg ccgccaccgc ggtggagctc caattcgccc
     4201 tatagtgagt cgtattacaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     4261 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     4321 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     4381 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     4441 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     4501 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     4561 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     4621 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     4681 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     4741 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     4801 acgcgaattt taacaaaata ttaacgctta caatttcctg atgcggtatt ttctccttac
     4861 gcatctgtgc ggtatttcac accgcatatc gaccggtcga ggagaacttc tagtatatct
     4921 acatacctaa tattattgcc ttattaaaaa tggaatccca acaattacat caaaatccac
     4981 attctcttca aaatcaattg tcctgtactt ccttgttcat gtgtgttcaa aaacgttata
     5041 tttataggat aattatactc tatttctcaa caagtaattg gttgtttggc cgagcggtct
     5101 aaggcgcctg attcaagaaa tatcttgacc gcagttaact gtgggaatac tcaggtatcg
     5161 taagatgcaa gagttcgaat ctcttagcaa ccattatttt tttcctcaac ataacgagaa
     5221 cacacagggg cgctatcgca cagaatcaaa ttcgatgact ggaaattttt tgttaatttc
     5281 agaggtcgcc tgacgcatat acctttttca actgaaaaat tgggagaaaa aggaaaggtg
     5341 agagcgccgg aaccggcttt tcatatagaa tagagaagcg ttcatgacta aatgcttgca
     5401 tcacaatact tgaagttgac aatattattt aaggacctat tgttttttcc aataggtggt
     5461 tagcaatcgt cttactttct aacttttctt accttttaca tttcagcaat atatatatat
     5521 atatttcaag gatataccat tctaatgtct gcccctaaga agatcgtcgt tttgccaggt
     5581 gaccacgttg gtcaagaaat cacagccgaa gccattaagg ttcttaaagc tatttctgat
     5641 gttcgttcca atgtcaagtt cgatttcgaa aatcatttaa ttggtggtgc tgctatcgat
     5701 gctacaggtg ttccacttcc agatgaggcg ctggaagcct ccaagaaggc tgatgccgtt
     5761 ttgttaggtg ctgtgggtgg tcctaaatgg ggtaccggta gtgttagacc tgaacaaggt
     5821 ttactaaaaa tccgtaaaga acttcaattg tacgccaact taagaccatg taactttgca
     5881 tccgactctc ttttagactt atctccaatc aagccacaat ttgctaaagg tactgacttc
     5941 gttgttgtca gagaattagt gggaggtatt tactttggta agagaaagga agacgatggt
     6001 gatggtgtcg cttgggatag tgaacaatac accgttccag aagtgcaaag aatcacaaga
     6061 atggccgctt tcatggccct acaacatgag ccaccattgc ctatttggtc cttggataaa
     6121 gctaatgttt tggcctcttc aagattatgg agaaaaactg tggaggaaac catcaagaac
     6181 gaattcccta cattgaaggt tcaacatcaa ttgattgatt ctgccgccat gatcctagtt
     6241 aagaacccaa cccacctaaa tggtattata atcaccagca acatgtttgg tgatatcatc
     6301 tccgatgaag cctccgttat cccaggttcc ttgggtttgt tgccatctgc gtccttggcc
     6361 tctttgccag acaagaacac cgcatttggt ttgtacgaac catgccacgg ttctgctcca
     6421 gatttgccaa agaataaggt caaccctatc gccactatct tgtctgctgc aatgatgttg
     6481 aaattgtcat tgaacttgcc tgaagaaggt aaggccattg aagatgcagt taaaaaggtt
     6541 ttggatgcag gtatcagaac tggtgattta ggtggttcca acagtaccac cgaagtcggt
     6601 gatgctgtcg ccgaagaagt taagaaaatc cttgcttaaa aagattctct ttttttatga
     6661 tatttgtaca taaactttat aaatgaaatt cataatagaa acgacacgaa attacaaaat
     6721 ggaatatgtt catagggtag acgaaactat atacgcaatc tacatacatt tatcaagaag
     6781 gagaaaaagg aggatgtaaa ggaatacagg taagcaaatt gatactaatg gctcaacgtg
     6841 ataaggaaaa agaattgcac tttaacatta atattgacaa ggaggagggc accacacaaa
     6901 aagttaggtg taacagaaaa tcatgaaact atgattccta atttatatat tggaggattt
     6961 tctctaaaaa aaaaaaaata caacaaataa aaaacactca atgacctgac catttgatgg
     7021 agtttaagtc aataccttct tgaaccattt cccataatgg tgaaagttcc ctcaagaatt
     7081 ttactctgtc agaaacggcc ttaacgacgt agtcgatatg gtgcactctc agtacaatct
     7141 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
     7201 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     7261 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga
     7321 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttaggac ggatcgcttg
     7381 cctgtaactt acacgcgcct cgtatctttt aatgatggaa taatttggga atttactctg
     7441 tgtttattta tttttatgtt ttgtatttgg attttagaaa gtaaataaag aaggtagaag
     7501 agttacggaa tgaagaaaaa aaaataaaca aaggtttaaa aaatttcaac aaaaagcgta
     7561 ctttacatat atatttatta gacaagaaaa gcagattaaa tagatataca ttcgattaac
     7621 gataagtaaa atgtaaaatc acaggatttt cgtgtgtggt cttctacaca gacaagatga
     7681 aacaattcgg cattaatacc tgagagcagg aagagcaaga taaaaggtag tatttgttgg
     7741 cgatccccct agagtctttt acatcttcgg aaaacaaaaa ctattttttc tttaatttct
     7801 ttttttactt tctattttta atttatatat ttatattaaa aaatttaaat tataattatt
     7861 tttatagcac gtgatgaaaa ggacccaggt ggcacttttc ggggaaatgt gcgcggaacc
     7921 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     7981 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     8041 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     8101 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     8161 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     8221 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     8281 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     8341 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     8401 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     8461 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     8521 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     8581 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     8641 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     8701 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     8761 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     8821 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     8881 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     8941 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     9001 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     9061 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     9121 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     9181 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     9241 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     9301 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     9361 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     9421 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     9481 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     9541 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     9601 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     9661 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     9721 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     9781 accgagcgca gcgagtcagt gagcgagga
//
