LOCUS       sequence-240344-        9809 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expressing GFP-Atg13[3SD].
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9809)
  AUTHORS   Chew LH, Lu S, Liu X, Li FK, Yu AY, Klionsky DJ, Dong MQ, Yip CK
  TITLE     Molecular interactions of the Saccharomyces cerevisiae Atg1 complex 
            provide insights into assembly and regulatory mechanisms.
  JOURNAL   Autophagy. 2015;11(6):891-905. doi: 10.1080/15548627.2015.1040972.
  PUBMED    25998554
REFERENCE   2  (bases 1 to 9809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Autophagy."; date: "2015"; volume: "11(6):891-905. doi"; pages: " 
            10.1080/15548627.2015.1040972"
COMMENT     Sequence Label: pGFP-ATG13[3SD](315)
FEATURES             Location/Qualifiers
     source          1..9809
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     34..51
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    168..189
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(204..209,210..227,228..234)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    242..258
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     247..269
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     266..282
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     266..282
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        303..321
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     349..365
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     350..366
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             484..1197
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFAYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             484..1197
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFAYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(514..542)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1137..1157
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(4251..4270)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4252..4270)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4277..4294)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4277..4293)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4286..4308)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4434..4889
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4521..4540)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4731..4752
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5190..5597
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             5598..6692
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(7186..7205)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7305..7327
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7365..7383)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    7425..7928
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        7965..8069
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8070..8138,8139..8930)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8288..8307)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9101..9689
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9590..9609
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
       61 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
      121 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
      181 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
      241 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagctc
      301 gaaattaacc ctcactaaag ggaacaaaag ctggtaccgg gccccccctc gaggtcgacg
      361 gtatcgataa gcttgatatc gaattcatag gtaccaaata cattccactt acaccttcca
      421 ggctcaagtc ttgaaaagaa agcagaacat acagcccggt tgaatagcat gagtctgcct
      481 agaatgagta aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat
      541 ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac
      601 ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca
      661 cttgtcacta ctttcgcgta tggtcttcaa tgctttgcga gatacccaga tcatatgaaa
      721 cagcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag aactatattt
      781 ttcaaagatg acgggaacta caagacacgt gctgaagtca agtttgaagg tgataccctt
      841 gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat tcttggacac
      901 aaattggaat acaactataa ctcacacaat gtatacatca tggcagacaa acaaaagaat
      961 ggaatcaaag ttaacttcaa aattagacac aacattgaag atggaagcgt tcaactagca
     1021 gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat
     1081 tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaaagagaga ccacatggtc
     1141 cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgaact atacaaatcg
     1201 tctagggttg ccgaagagga catcgagaag caagtccttc aattgataga cagctttttt
     1261 ctgaagacta cactactaat atgctccacc gaatcaagtc gataccagtc ttctacagaa
     1321 aatatattcc tatttgacga cacatggttt gaagatcact cagaattagt gagtgagcta
     1381 cccgagataa tatcaaaatg gtctcactac gatggtcgaa aagagttgcc acccttagtg
     1441 gtagagacat atttggattt aagacagtta aactcgtctc atttagttag attaaaggac
     1501 cacgaaggcc atttgtggaa cgtttgcaaa ggaactaaga agcaggaaat cgtgatggaa
     1561 cgttggctta tcgaattaga taattcatcc ccaactttca aatcatacag tgaagatgag
     1621 actgatgtta atgaactttc taaacagcta gtccttctct tccgttattt gttgacttta
     1681 atacagttac tacccacaac agaattatac caattattaa taaagtctta taacggcccg
     1741 caaaatgaag gaagttccaa tccaataact tccacgggcc cactagtaag tatccggacg
     1801 tgtgtccttg acggatctaa accaatttta tcgaagggga gaatagggtt gagcaaaccg
     1861 attattaata catattccaa tgcgcttaac gaatcaaacc tgccagccca tttagatcaa
     1921 aagaagatca cacctgtatg gacaaagttt ggactcttaa gagtctcggt atcatacaga
     1981 cgtgattgga agtttgaaat taacaataca aacgacgaat tattttcagc tcgacatgca
     2041 tctgtctcac ataactcaca aggaccccag aatcagccag aacaagaagg acaaagtgat
     2101 caagacatag ggaaacgcca accacaattt caacagcagc agcagcccca acagcagcag
     2161 cagcagcagc aacagcaaca gagacaacac caggtccaga cacaacaaca aagacagata
     2221 cctgatagga gatctctttc actttctcct tgtacaagag ccaattcttt tgaaccacaa
     2281 tcttggcaga agaaagtcta tccaatatcg agacctgttc aaccatttaa agttggttca
     2341 attggaagtc aaagtgcgag cagaaatccc tctaattcat cgtttttcaa ccaaccacct
     2401 gttcataggc caagtatgag ctccaactac gggccacaaa tgaatattga aggtaccagt
     2461 gttggaagca cctcaaagta ttcctcctcc tttgggaaca ttcgtcgtca ctcaagtgta
     2521 aagacgacag agaatgctga aaaagtatca aaagctgtaa agagcccact acaacctcaa
     2581 gaatcacaag aagatttaat ggattttgtt aaattactcg aagaaaaacc cgatctaact
     2641 ataaagaaga caagtggaaa taatccaccc aatatcaata tttctgattc tctaatcaga
     2701 tatcagaatt tgaagccaag taatgactta ttaagtgaag atttatccgt aagtttatcc
     2761 atggatccaa atcatacata tcacagaggc agatcagatt cccactcacc attgccttca
     2821 atatcccctt cgatgcatta tggatcgttg aactcgagaa tgtctcaagg cgccaatgca
     2881 agccatttga ttgcaagagg cggtgggaat tcatctacta gtgccttgaa tagtagaagg
     2941 aattctttag ataagagctc aaacaagcag ggtatgtcag gcttacctcc tatttttggt
     3001 ggagagagta cttcatatca ccacgacaac aaaatacaaa agtacaacca attaggagta
     3061 gaagaagatg atgatgacga gaatgaccgt ttgctcaacc aaatgggaaa cagtgctaca
     3121 aaattcaaaa gttcaataga cccaagagac attgatgaca tttcaagttc tttcataaaa
     3181 agtaggatac ctatcagaca accataccat tactctcaac caactactgc gccctttcaa
     3241 gctcaggcga aatttcataa acctgcaaat aagttaatcg ataatggtaa taggagtaat
     3301 agtaacaata acaatcataa tgggaatgat gcagttggtg tgatgcataa tgacgaggat
     3361 gatcaagatg atgatctagt atttttcatg agtgatatga acctttctaa agaaggttaa
     3421 aaatggtaaa gttttatttt aaagggcaca actaaagaaa aataatcaaa aaaaaggtga
     3481 aatagatatg caagaaaaga aaaaaaaata caatactagt aaactgaaat aaaaaagatt
     3541 agccataatg gatttgtttc tctttgttat cgatattctt acctcttgaa gatccatgtg
     3601 tctatcattt gaattgaata tataattata tacagtgaga tataccaatt gcagtaacaa
     3661 aatggcaaat atgtatatca gtaatataat tagcatataa aataaaaaaa aaaaatgctt
     3721 atttgtcacc cgctgactgc gtttcctttt gaattataaa aggttcctta tggttctggt
     3781 ccactagcgt tttattttct tccttttcct gtaatatttt taactttagc tcatgtactt
     3841 tgtgcttgtc tatatgtttc tcgtattttt cctttgtctt gaaagtccta gaacaattat
     3901 tgtaaaagca tctgtatttt cttccaacgg tattcaataa aaggtttagc ccattttcac
     3961 cagactcaag tcgcttttct aaatccactt cattctgtaa aaactctaca ttattgtttt
     4021 cggtaagttt tctccttttc ctcaacctat tggaaatctt ctcttcatcc tgctcattcg
     4081 agtgtttgct attccccagt tgaactccgt ggtcttggac aagttgttga atatcagaaa
     4141 ttttatattt tagttccagt gggatatctt cttccgtatg aatagaccca taatgcgtga
     4201 gaagatcatg ttttctagag cggccgccac cgcggtggag ctccaattcg ccctatagtg
     4261 agtcgtatta caattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     4321 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     4381 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggacgcgcc
     4441 ctgtagcggc gcattaagtg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     4501 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     4561 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     4621 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     4681 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     4741 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     4801 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     4861 ttttaacaaa atattaacgc ttacaatttc ctgatgcggt attttctcct tacgcatctg
     4921 tgcggtattt cacaccgcat atcgaccggt cgaggagaac ttctagtata tctacatacc
     4981 taatattatt gccttattaa aaatggaatc ccaacaatta catcaaaatc cacattctct
     5041 tcaaaatcaa ttgtcctgta cttccttgtt catgtgtgtt caaaaacgtt atatttatag
     5101 gataattata ctctatttct caacaagtaa ttggttgttt ggccgagcgg tctaaggcgc
     5161 ctgattcaag aaatatcttg accgcagtta actgtgggaa tactcaggta tcgtaagatg
     5221 caagagttcg aatctcttag caaccattat ttttttcctc aacataacga gaacacacag
     5281 gggcgctatc gcacagaatc aaattcgatg actggaaatt ttttgttaat ttcagaggtc
     5341 gcctgacgca tatacctttt tcaactgaaa aattgggaga aaaaggaaag gtgagagcgc
     5401 cggaaccggc ttttcatata gaatagagaa gcgttcatga ctaaatgctt gcatcacaat
     5461 acttgaagtt gacaatatta tttaaggacc tattgttttt tccaataggt ggttagcaat
     5521 cgtcttactt tctaactttt cttacctttt acatttcagc aatatatata tatatatttc
     5581 aaggatatac cattctaatg tctgccccta agaagatcgt cgttttgcca ggtgaccacg
     5641 ttggtcaaga aatcacagcc gaagccatta aggttcttaa agctatttct gatgttcgtt
     5701 ccaatgtcaa gttcgatttc gaaaatcatt taattggtgg tgctgctatc gatgctacag
     5761 gtgttccact tccagatgag gcgctggaag cctccaagaa ggctgatgcc gttttgttag
     5821 gtgctgtggg tggtcctaaa tggggtaccg gtagtgttag acctgaacaa ggtttactaa
     5881 aaatccgtaa agaacttcaa ttgtacgcca acttaagacc atgtaacttt gcatccgact
     5941 ctcttttaga cttatctcca atcaagccac aatttgctaa aggtactgac ttcgttgttg
     6001 tcagagaatt agtgggaggt atttactttg gtaagagaaa ggaagacgat ggtgatggtg
     6061 tcgcttggga tagtgaacaa tacaccgttc cagaagtgca aagaatcaca agaatggccg
     6121 ctttcatggc cctacaacat gagccaccat tgcctatttg gtccttggat aaagctaatg
     6181 ttttggcctc ttcaagatta tggagaaaaa ctgtggagga aaccatcaag aacgaattcc
     6241 ctacattgaa ggttcaacat caattgattg attctgccgc catgatccta gttaagaacc
     6301 caacccacct aaatggtatt ataatcacca gcaacatgtt tggtgatatc atctccgatg
     6361 aagcctccgt tatcccaggt tccttgggtt tgttgccatc tgcgtccttg gcctctttgc
     6421 cagacaagaa caccgcattt ggtttgtacg aaccatgcca cggttctgct ccagatttgc
     6481 caaagaataa ggtcaaccct atcgccacta tcttgtctgc tgcaatgatg ttgaaattgt
     6541 cattgaactt gcctgaagaa ggtaaggcca ttgaagatgc agttaaaaag gttttggatg
     6601 caggtatcag aactggtgat ttaggtggtt ccaacagtac caccgaagtc ggtgatgctg
     6661 tcgccgaaga agttaagaaa atccttgctt aaaaagattc tcttttttta tgatatttgt
     6721 acataaactt tataaatgaa attcataata gaaacgacac gaaattacaa aatggaatat
     6781 gttcataggg tagacgaaac tatatacgca atctacatac atttatcaag aaggagaaaa
     6841 aggaggatgt aaaggaatac aggtaagcaa attgatacta atggctcaac gtgataagga
     6901 aaaagaattg cactttaaca ttaatattga caaggaggag ggcaccacac aaaaagttag
     6961 gtgtaacaga aaatcatgaa actatgattc ctaatttata tattggagga ttttctctaa
     7021 aaaaaaaaaa atacaacaaa taaaaaacac tcaatgacct gaccatttga tggagtttaa
     7081 gtcaatacct tcttgaacca tttcccataa tggtgaaagt tccctcaaga attttactct
     7141 gtcagaaacg gccttaacga cgtagtcgat atggtgcact ctcagtacaa tctgctctga
     7201 tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     7261 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     7321 tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct
     7381 atttttatag gttaatgtca tgataataat ggtttcttag gacggatcgc ttgcctgtaa
     7441 cttacacgcg cctcgtatct tttaatgatg gaataatttg ggaatttact ctgtgtttat
     7501 ttatttttat gttttgtatt tggattttag aaagtaaata aagaaggtag aagagttacg
     7561 gaatgaagaa aaaaaaataa acaaaggttt aaaaaatttc aacaaaaagc gtactttaca
     7621 tatatattta ttagacaaga aaagcagatt aaatagatat acattcgatt aacgataagt
     7681 aaaatgtaaa atcacaggat tttcgtgtgt ggtcttctac acagacaaga tgaaacaatt
     7741 cggcattaat acctgagagc aggaagagca agataaaagg tagtatttgt tggcgatccc
     7801 cctagagtct tttacatctt cggaaaacaa aaactatttt ttctttaatt tcttttttta
     7861 ctttctattt ttaatttata tatttatatt aaaaaattta aattataatt atttttatag
     7921 cacgtgatga aaaggaccca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     7981 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     8041 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     8101 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     8161 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     8221 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     8281 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     8341 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     8401 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     8461 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     8521 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     8581 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     8641 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     8701 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     8761 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     8821 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     8881 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ttgtcagacc
     8941 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     9001 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     9061 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     9121 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     9181 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     9241 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     9301 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     9361 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     9421 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     9481 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     9541 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     9601 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     9661 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     9721 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     9781 ataaccgtat taccgccttt gagtgagct
//
