LOCUS       sequence-240349-       11602 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the BaL HIV-1 isolate; 
            C-terminal truncation; QES mutant with enhanced binding to PG16.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11602)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 11602)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-BaL-QES.i01.c08-754*
FEATURES             Location/Qualifiers
     source          1..11602
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(677..695)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        763..867
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(868..936,937..1728)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1086..1105)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1899..2487
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2388..2407
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2918..3063
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3106..4143
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4141..4160)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4185..4233
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4887..5266
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5267..5470
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5421..5441
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5467..5491
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             7466..7510
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     polyA_signal    7788..7922
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7812..7831)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7866..7885
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(8065..8087)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8647..10436
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 ccaatgcaac ttggacgttt ttggggtctc cggacaccat ctctatgtct tggccctgat
       61 cctgagccgc ccggggctcc tggtcttccg cctcctcgtc ctcgtcctct tccccgtcct
      121 cgtccatggt tatcaccccc tcttctttga ggtccactgc cgccggagcc ttctggtcca
      181 gatgtgtctc ccttctctcc taggccattt ccaggtcctg tacctggccc ctcgtcagac
      241 atgattcaca ctaaaagaga tcaatagaca tctttattag acgacgctca gtgaatacag
      301 ggagtgcaga ctcctgcccc ctccaacagc ccccccaccc tcatcccctt catggtcgct
      361 gtcagacaga tccaggtctg aaaattcccc atcctccgaa ccatcctcgt cctcatcacc
      421 aattactcgc agcccggaaa actcccgctg aacatcctca agatttgcgt cctgagcctc
      481 aagccaggcc tcaaattcct cgtccccctt tttgctggac ggtagggatg gggattctcg
      541 ggacccctcc tcttcctctt caaggtcacc agacagagat gctactgggg caacggaaga
      601 aaagctgggt gcggcctgtg aggatcagct tatcgatgat aagctgtcaa acatgagaat
      661 tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
      721 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
      781 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
      841 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
      901 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
      961 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     1021 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     1081 gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc
     1141 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     1201 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     1261 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     1321 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     1381 ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta
     1441 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     1501 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     1561 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     1621 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     1681 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     1741 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     1801 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     1861 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     1921 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     1981 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     2041 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     2101 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     2161 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     2221 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     2281 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     2341 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     2401 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     2461 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     2521 gccttttgct ggccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg
     2581 gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc
     2641 atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cccgagatgc
     2701 gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt
     2761 gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt
     2821 tagcgaggtg ccgccctgct tcatccccgt ggcccgttgc tcgcgtttgc tggcggtgtc
     2881 cccggaagaa atatatttgc atgtctttag ttctatgatg acacaaaccc cgcccagcgt
     2941 cttgtcattg gcgaattcga acacgcagat gcagtcgggg cggcgcggtc cgaggtccac
     3001 ttcgcatatt aaggtgacgc gtgtggcctc gaacaccgag cgaccctgca gcgacccgct
     3061 taacagcgtc aacagcgtgc cgcagatccc ggggggcaat gagatatgaa aaagcctgaa
     3121 ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg
     3181 atgcagctct cggagggcga agaatctcgt gctttcagct tcgatgtagg agggcgtgga
     3241 tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg
     3301 cactttgcat cggccgcgct cccgattccg gaagtgcttg acattgggga attcagcgag
     3361 agcctgacct attgcatctc ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa
     3421 accgaactgc ccgctgttct gcagccggtc gcggaggcca tggatgcgat cgctgcggcc
     3481 gatcttagcc agacgagcgg gttcggccca ttcggaccgc aaggaatcgg tcaatacact
     3541 acatggcgtg atttcatatg cgcgattgct gatccccatg tgtatcactg gcaaactgtg
     3601 atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat gctttgggcc
     3661 gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa caatgtcctg
     3721 acggacaatg gccgcataac agcggtcatt gactggagcg aggcgatgtt cggggattcc
     3781 caatacgagg tcgccaacat cttcttctgg aggccgtggt tggcttgtat ggagcagcag
     3841 acgcgctact tcgagcggag gcatccggag cttgcaggat cgccgcggct ccgggcgtat
     3901 atgctccgca ttggtcttga ccaactctat cagagcttgg ttgacggcaa tttcgatgat
     3961 gcagcttggg cgcagggtcg atgcgacgca atcgtccgat ccggagccgg gactgtcggg
     4021 cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt agaagtactc
     4081 gccgatagtg gaaaccgacg ccccagcact cgtccggatc gggagatggg ggaggctaac
     4141 tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca
     4201 gaataaaacg cacgggtgtt gggtcgtttg ttcataaacg cggggttcgg tcccagggct
     4261 ggcactctgt cgatacccca ccgagacccc attggggcca atacgcccgc gtttcttcct
     4321 tttccccacc ccacccccca agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg
     4381 cggcaggccc tgccatagcc actggccccg tgggttaggg acggggtccc ccatggggaa
     4441 tggtttatgg ttcgtggggg ttattatttt gggcgttgcg tggggtcagg tccacgactg
     4501 gactgagcag acagacccat ggtttttgga tggcctgggc atggaccgca tgtactggcg
     4561 cgacacgaac accgggcgtc tgtggctgcc aaacaccccc gacccccaaa aaccaccgcg
     4621 cggatttctg gcgtgccaag ctagtcgacc aattctcatg tttgacagct tatcatcgca
     4681 gatccgggca acgttgttgc cattgctgca ggcgcagaac tggtaggtat ggaagatcta
     4741 tacattgaat caatattggc aattagccat attagtcatt ggttatatag cataaatcaa
     4801 tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg
     4861 ctcatgtcca atatgaccgc catgttgaca ttgattattg actagttatt aatagtaatc
     4921 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     4981 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     5041 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     5101 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtccgc cccctattga
     5161 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tacgggactt
     5221 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     5281 gcagtacacc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     5341 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     5401 taataacccc gccccgttga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     5461 aagcagagct cgtttagtga accgtcagat ctctagaagc tgggtaccag ctgctagcgc
     5521 caccatgccc atggggagct tgcaaccgct cgccactctc tatctcctcg gcatgttggt
     5581 agcatctgtg ctggctgagg aaaagctctg ggtgactgtg tattacgggg tcccagtttg
     5641 gaaggaggct gataccacgc tcttctgcgc ctccgaccgg aaggcatacg acaccgaggt
     5701 gcataatgtg tgggccacac acgcctgtgt gcctaccgac cccaacccac aggaagtgga
     5761 actgaagaac gtgactgaga atttcaatat gtggaaaaat aatatggtgg agcagatgca
     5821 cgaggacatt attagcctct gggatcagtc ccttaaaccc tgcgtgaaac tgacagatct
     5881 gtgcgtaacg ctgaattgta ctgacctccg gaatgccacg aacggaaacg acacaaatac
     5941 cacgtcaagc tcccgaggga tggtgggagg gggagaaatg aagaactgct cattcaatat
     6001 aacgacgaat atccgaggga aggttcagaa agagtatgcc ctgttttaca aactggacct
     6061 cgctcctatc gacaacaaca gcaataacag gtaccgcctg attagctgca acacttctgt
     6121 gattacccag gcctgcccca aggtgagctt cgagcctatc ccaatccact actgcgcccc
     6181 agcaggcttt gccatcctga aatgcaagga caagaagttc aacggcaaag gcccatgtac
     6241 aaatgtcagt acagtacagt gcacacacgg tatccgccca gttatgtcta cacaactgct
     6301 gctgaatgga tccttggcag aggaagaggt ggtgatcaga tctgctaact tcgccgataa
     6361 tgctaaaccc atcattgttc agctgaacga gtctgtcgaa atcaattgta ctcgcccaaa
     6421 caacaacacg cgcaagagca ttcatatcgg accgggcagg gccttttaca caacagggga
     6481 gattattggt gatattagac aagcccactg caacctcagt cgagcaaagt ggaatgatac
     6541 tctcaacaaa attgttatca aattgcggga acagtttggg aacaagacaa tcgtatttaa
     6601 gcactcatcc ggaggcgatc cagaaatcgt gacacactct tttaactgcg gtggagaatt
     6661 tttctactgc aactccaccc agctgttcaa cagtacgtgg aacgtgacag aggagtctaa
     6721 caacaccgtg gagaataata cgatcaccct tccatgtcgg attaagcaga tcatcaatat
     6781 gtggcaggag gtgggaagag caatgtacgc gccgcccatt aggggccaga tcagatgcag
     6841 ttcaaatatc acaggcctgc tcctgacacg cgacggaggc ccagaggaca acaagacaga
     6901 ggtgtttagg cccggcggcg gcgacatgcg agataattgg aggtctgaat tgtacaagta
     6961 caaggtggtg aaaatcgaac ccctgggagt ggcgcctact aaggcaaagc gccgcgttgt
     7021 gcagagagag aagagggcag tgggaattgg ggcggttttt cttgggttcc tgggggctgc
     7081 aggtagcacc atgggagcgg ccgctatgac actgacagtg caagccagac tcctgctgtc
     7141 tggtattgtc caacagcaaa acaatttgct ccgcgcaata gaagcccagc agcacctgct
     7201 ccaactgact gtgtggggca ttaaacaatt gcaggctcgc gtgctggcag tcgaacggta
     7261 cctgagggat caacagctcc tgggcatgtg gggctgctcc gggaaactca tctgtacgac
     7321 agcggtccct tggaatgcca gctggtccaa caaaagcctc aacaagattt gggacaatat
     7381 gacgtggatt gagtgggaca gggaaattaa caattatacc tcaatcatat attctctgat
     7441 tgaagagtcc cagaatcagc aagagaagaa tgagcaggaa ttgttggaac ttgataaatg
     7501 ggcaagcctg tggaactggt ttgacataac caaatggttg tggtatatca aaatcttcat
     7561 aatgatcgtc ggcggactca tcgggctccg aattgtgttc tcagtcctca gcattgtgaa
     7621 tagggtgcgc cagggctatt ctcccctgag cttccagacc catctgccct ctagccgagg
     7681 accagatagg cctggtggga tcgaggaaga aggcggggaa cgggacaggg ataggagtgg
     7741 tcctcttgtt aacgggttcc tgtgataact cgaggccggc aaggccggat ccagacatga
     7801 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
     7861 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
     7921 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
     7981 tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatccg gctgcctcgc
     8041 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
     8101 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
     8161 cgggtgtcgg ggcgcagcca tgaggtcgac tctagaggat cgatccccgc cccggacgaa
     8221 ctaaacctga ctacgacatc tctgcccctt cttcgcgggg cagtgcatgt aatcccttca
     8281 gttggttggt acaacttgcc aactgggccc tgttccacat gtgacacggg gggggaccaa
     8341 acacaaaggg gttctctgac tgtagttgac atccttataa atggatgtgc acatttgcca
     8401 acactgagtg gctttcatcc tggagcagac tttgcagtct gtggactgca acacaacatt
     8461 gcctttatgt gtaactcttg gctgaagctc ttacaccaat gctgggggac atgtacctcc
     8521 caggggccca ggaagactac gggaggctac accaacgtca atcagagggg cctgtgtagc
     8581 taccgataag cggaccctca agagggcatt agcaatagtg tttataaggc ccccttgtta
     8641 accctaaacg ggtagcatat gcttcccggg tagtagtata tactatccag actaacccta
     8701 attcaatagc atatgttacc caacgggaag catatgctat cgaattaggg ttagtaaaag
     8761 ggtcctaagg aacagcgata tctcccaccc catgagctgt cacggtttta tttacatggg
     8821 gtcaggattc cacgagggta gtgaaccatt ttagtcacaa gggcagtggc tgaagatcaa
     8881 ggagcgggca gtgaactctc ctgaatcttc gcctgcttct tcattctcct tcgtttagct
     8941 aatagaataa ctgctgagtt gtgaacagta aggtgtatgt gaggtgctcg aaaacaaggt
     9001 ttcaggtgac gcccccagaa taaaatttgg acggggggtt cagtggtggc attgtgctat
     9061 gacaccaata taaccctcac aaaccccttg ggcaataaat actagtgtag gaatgaaaca
     9121 ttctgaatat ctttaacaat agaaatccat ggggtgggga caagccgtaa agactggatg
     9181 tccatctcac acgaatttat ggctatgggc aacacataat cctagtgcaa tatgatactg
     9241 gggttattaa gatgtgtccc aggcagggac caagacaggt gaaccatgtt gttacactct
     9301 atttgtaaca aggggaaaga gagtggacgc cgacagcagc ggactccact ggttgtctct
     9361 aacacccccg aaaattaaac ggggctccac gccaatgggg cccataaaca aagacaagtg
     9421 gccactcttt tttttgaaat tgtggagtgg gggcacgcgt cagcccccac acgccgccct
     9481 gcggttttgg actgtaaaat aagggtgtaa taacttggct gattgtaacc ccgctaacca
     9541 ctgcggtcaa accacttgcc cacaaaacca ctaatggcac cccggggaat acctgcataa
     9601 gtaggtgggc gggccaagat aggggcgcga ttgctgcgat ctggaggaca aattacacac
     9661 acttgcgcct gagcgccaag cacagggttg ttggtcctca tattcacgag gtcgctgaga
     9721 gcacggtggg ctaatgttgc catgggtagc atatactacc caaatatctg gatagcatat
     9781 gctatcctaa tctatatctg ggtagcatag gctatcctaa tctatatctg ggtagcatat
     9841 gctatcctaa tctatatctg ggtagtatat gctatcctaa tttatatctg ggtagcatag
     9901 gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagtatat
     9961 gctatcctaa tctgtatccg ggtagcatat gctatcctaa tagagattag ggtagtatat
    10021 gctatcctaa tttatatctg ggtagcatat actacccaaa tatctggata gcatatgcta
    10081 tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta gcataggcta
    10141 tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta gtatatgcta
    10201 tcctaattta tatctgggta gcataggcta tcctaatcta tatctgggta gcatatgcta
    10261 tcctaatcta tatctgggta gtatatgcta tcctaatctg tatccgggta gcatatgcta
    10321 tcctcatgca tatacagtca gcatatgata cccagtagta gagtgggagt gctatccttt
    10381 gcatatgccg ccacctccca agggggcgtg aattttcgct gcttgtcctt ttcctgctgg
    10441 ttgctcccat tcttaggtga atttaaggag gccaggctaa agccgtcgca tgtctgattg
    10501 ctcaccaggt aaatgtcgct aatgttttcc aacgcgagaa ggtgttgagc gcggagctga
    10561 gtgacgtgac aacatgggta tgcccaattg ccccatgttg ggaggacgaa aatggtgaca
    10621 agacagatgg ccagaaatac accaacagca cgcatgatgt ctactgggga tttattcttt
    10681 agtgcggggg aatacacggc ttttaatacg attgagggcg tctcctaaca agttacatca
    10741 ctcctgccct tcctcaccct catctccatc acctccttca tctccgtcat ctccgtcatc
    10801 accctccgcg gcagcccctt ccaccatagg tggaaaccag ggaggcaaat ctactccatc
    10861 gtcaaagctg cacacagtca ccctgatatt gcaggtagga gcgggctttg tcataacaag
    10921 gtccttaatc gcatccttca aaacctcagc aaatatatga gtttgtaaaa agaccatgaa
    10981 ataacagaca atggactccc ttagcgggcc aggttgtggg ccgggtccag gggccattcc
    11041 aaaggggaga cgactcaatg gtgtaagacg acattgtgga atagcaaggg cagttcctcg
    11101 ccttaggttg taaagggagg tcttactacc tccatatacg aacacaccgg cgacccaagt
    11161 tccttcgtcg gtagtccttt ctacgtgact cctagccagg agagctctta aaccttctgc
    11221 aatgttctca aatttcgggt tggaacctcc ttgaccacga tgctttccaa accaccctcc
    11281 ttttttgcgc ctgcctccat caccctgacc ccggggtcca gtgcttgggc cttctcctgg
    11341 gtcatctgcg gggccctgct ctatcgctcc cgggggcacg tcaggctcac catctgggcc
    11401 accttcttgg tggtattcaa aataatcggc ttcccctaca gggtggaaaa atggccttct
    11461 acctggaggg ggcctgcgcg gtggagaccc ggatgatgat gactgactac tgggactcct
    11521 gggcctcttt tctccacgtc cacgacctct ccccctggct ctttcacgac ttccccccct
    11581 ggctctttca cgtcctctac cc
//
