LOCUS       sequence-240350-       11496 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the BaL HIV-1 isolate; QES
            mutant with enhanced binding to PG16.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11496)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 11496)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-BaL-QES.i01.c08
FEATURES             Location/Qualifiers
     source          1..11496
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      1086..2875
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     3435..3457
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     polyA_signal    3600..3734
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3637..3656)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3691..3710
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(4324..4368)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     complement(6343..6367)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(6364..6567)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(6393..6413)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        6568..6947
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     polyA_signal    7601..7649
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     7674..7693
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(7691..8728)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     promoter        complement(8771..8916)
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     rep_origin      complement(9347..9935)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9427..9446)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10106..10897,10898..10966))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10729..10748
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10967..11071)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11139..11157
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 caggagtccc agtagtcagt catcatcatc cgggtctcca ccgcgcaggc cccctccagg
       61 tagaaggcca tttttccacc ctgtagggga agccgattat tttgaatacc accaagaagg
      121 tggcccagat ggtgagcctg acgtgccccc gggagcgata gagcagggcc ccgcagatga
      181 cccaggagaa ggcccaagca ctggaccccg gggtcagggt gatggaggca ggcgcaaaaa
      241 aggagggtgg tttggaaagc atcgtggtca aggaggttcc aacccgaaat ttgagaacat
      301 tgcagaaggt ttaagagctc tcctggctag gagtcacgta gaaaggacta ccgacgaagg
      361 aacttgggtc gccggtgtgt tcgtatatgg aggtagtaag acctcccttt acaacctaag
      421 gcgaggaact gcccttgcta ttccacaatg tcgtcttaca ccattgagtc gtctcccctt
      481 tggaatggcc cctggacccg gcccacaacc tggcccgcta agggagtcca ttgtctgtta
      541 tttcatggtc tttttacaaa ctcatatatt tgctgaggtt ttgaaggatg cgattaagga
      601 ccttgttatg acaaagcccg ctcctacctg caatatcagg gtgactgtgt gcagctttga
      661 cgatggagta gatttgcctc cctggtttcc acctatggtg gaaggggctg ccgcggaggg
      721 tgatgacgga gatgacggag atgaaggagg tgatggagat gagggtgagg aagggcagga
      781 gtgatgtaac ttgttaggag acgccctcaa tcgtattaaa agccgtgtat tcccccgcac
      841 taaagaataa atccccagta gacatcatgc gtgctgttgg tgtatttctg gccatctgtc
      901 ttgtcaccat tttcgtcctc ccaacatggg gcaattgggc atacccatgt tgtcacgtca
      961 ctcagctccg cgctcaacac cttctcgcgt tggaaaacat tagcgacatt tacctggtga
     1021 gcaatcagac atgcgacggc tttagcctgg cctccttaaa ttcacctaag aatgggagca
     1081 accagcagga aaaggacaag cagcgaaaat tcacgccccc ttgggaggtg gcggcatatg
     1141 caaaggatag cactcccact ctactactgg gtatcatatg ctgactgtat atgcatgagg
     1201 atagcatatg ctacccggat acagattagg atagcatata ctacccagat atagattagg
     1261 atagcatatg ctacccagat atagattagg atagcctatg ctacccagat ataaattagg
     1321 atagcatata ctacccagat atagattagg atagcatatg ctacccagat atagattagg
     1381 atagcctatg ctacccagat atagattagg atagcatatg ctacccagat atagattagg
     1441 atagcatatg ctatccagat atttgggtag tatatgctac ccagatataa attaggatag
     1501 catatactac cctaatctct attaggatag catatgctac ccggatacag attaggatag
     1561 catatactac ccagatatag attaggatag catatgctac ccagatatag attaggatag
     1621 cctatgctac ccagatataa attaggatag catatactac ccagatatag attaggatag
     1681 catatgctac ccagatatag attaggatag cctatgctac ccagatatag attaggatag
     1741 catatgctat ccagatattt gggtagtata tgctacccat ggcaacatta gcccaccgtg
     1801 ctctcagcga cctcgtgaat atgaggacca acaaccctgt gcttggcgct caggcgcaag
     1861 tgtgtgtaat ttgtcctcca gatcgcagca atcgcgcccc tatcttggcc cgcccaccta
     1921 cttatgcagg tattccccgg ggtgccatta gtggttttgt gggcaagtgg tttgaccgca
     1981 gtggttagcg gggttacaat cagccaagtt attacaccct tattttacag tccaaaaccg
     2041 cagggcggcg tgtgggggct gacgcgtgcc cccactccac aatttcaaaa aaaagagtgg
     2101 ccacttgtct ttgtttatgg gccccattgg cgtggagccc cgtttaattt tcgggggtgt
     2161 tagagacaac cagtggagtc cgctgctgtc ggcgtccact ctctttcccc ttgttacaaa
     2221 tagagtgtaa caacatggtt cacctgtctt ggtccctgcc tgggacacat cttaataacc
     2281 ccagtatcat attgcactag gattatgtgt tgcccatagc cataaattcg tgtgagatgg
     2341 acatccagtc tttacggctt gtccccaccc catggatttc tattgttaaa gatattcaga
     2401 atgtttcatt cctacactag tatttattgc ccaaggggtt tgtgagggtt atattggtgt
     2461 catagcacaa tgccaccact gaaccccccg tccaaatttt attctggggg cgtcacctga
     2521 aaccttgttt tcgagcacct cacatacacc ttactgttca caactcagca gttattctat
     2581 tagctaaacg aaggagaatg aagaagcagg cgaagattca ggagagttca ctgcccgctc
     2641 cttgatcttc agccactgcc cttgtgacta aaatggttca ctaccctcgt ggaatcctga
     2701 ccccatgtaa ataaaaccgt gacagctcat ggggtgggag atatcgctgt tccttaggac
     2761 ccttttacta accctaattc gatagcatat gcttcccgtt gggtaacata tgctattgaa
     2821 ttagggttag tctggatagt atatactact acccgggaag catatgctac ccgtttaggg
     2881 ttaacaaggg ggccttataa acactattgc taatgccctc ttgagggtcc gcttatcggt
     2941 agctacacag gcccctctga ttgacgttgg tgtagcctcc cgtagtcttc ctgggcccct
     3001 gggaggtaca tgtcccccag cattggtgta agagcttcag ccaagagtta cacataaagg
     3061 caatgttgtg ttgcagtcca cagactgcaa agtctgctcc aggatgaaag ccactcagtg
     3121 ttggcaaatg tgcacatcca tttataagga tgtcaactac agtcagagaa cccctttgtg
     3181 tttggtcccc ccccgtgtca catgtggaac agggcccagt tggcaagttg taccaaccaa
     3241 ctgaagggat tacatgcact gccccgcgaa gaaggggcag agatgtcgta gtcaggttta
     3301 gttcgtccgg ggcggggatc gatcctctag agtcgacctc atggctgcgc cccgacaccc
     3361 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     3421 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
     3481 cgcgaggcag ccggatcata atcagccata ccacatttgt agaggtttta cttgctttaa
     3541 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
     3601 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     3661 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     3721 atcatgtctg gatccggcct tgccggcctc gagttactac aggagagctc tctccagccc
     3781 ctgccgaatg cgtcgtggga tatgcagaat agcgcgaact gctcgttgca gtacttcaat
     3841 aactcgatcg gttccttcgg ccacagcaac agctgtggca ttaaggagac tcacagcaga
     3901 gttcttaagt tcctggctcc aatactgcaa cagattccac caatatttca gtacttccca
     3961 gccgccagca agccccaaca gctcgacgat ccgcatgact atcaggagaa gatcccggag
     4021 cctatgatag gagaacagga acaggcttct caagtccacc catataagtg ccaggaaccc
     4081 gttaacaaga ggaccactcc tatccctgtc ccgttccccg ccttcttcct cgatcccacc
     4141 aggcctatct ggtcctcggc tagagggcag atgggtctgg aagctcaggg gagaatagcc
     4201 ctggcgcacc ctattcacaa tgctgaggac tgagaacaca attcggagcc cgatgagtcc
     4261 gccgacgatc attatgaaga ttttgatata ccacaaccat ttggttatgt caaaccagtt
     4321 ccacaggctt gcccatttat caagttccaa caattcctgc tcattcttct cttgctgatt
     4381 ctgggactct tcaatcagag aatatatgat tgaggtataa ttgttaattt ccctgtccca
     4441 ctcaatccac gtcatattgt cccaaatctt gttgaggctt ttgttggacc agctggcatt
     4501 ccaagggacc gctgtcgtac agatgagttt cccggagcag ccccacatgc ccaggagctg
     4561 ttgatccctc aggtaccgtt cgactgccag cacgcgagcc tgcaattgtt taatgcccca
     4621 cacagtcagt tggagcaggt gctgctgggc ttctattgcg cggagcaaat tgttttgctg
     4681 ttggacaata ccagacagca ggagtctggc ttgcactgtc agtgtcatag cggccgctcc
     4741 catggtgcta cctgcagccc ccaggaaccc aagaaaaacc gccccaattc ccactgccct
     4801 cttctctctc tgcacaacgc ggcgctttgc cttagtaggc gccactccca ggggttcgat
     4861 tttcaccacc ttgtacttgt acaattcaga cctccaatta tctcgcatgt cgccgccgcc
     4921 gggcctaaac acctctgtct tgttgtcctc tgggcctccg tcgcgtgtca ggagcaggcc
     4981 tgtgatattt gaactgcatc tgatctggcc cctaatgggc ggcgcgtaca ttgctcttcc
     5041 cacctcctgc cacatattga tgatctgctt aatccgacat ggaagggtga tcgtattatt
     5101 ctccacggtg ttgttagact cctctgtcac gttccacgta ctgttgaaca gctgggtgga
     5161 gttgcagtag aaaaattctc caccgcagtt aaaagagtgt gtcacgattt ctggatcgcc
     5221 tccggatgag tgcttaaata cgattgtctt gttcccaaac tgttcccgca atttgataac
     5281 aattttgttg agagtatcat tccactttgc tcgactgagg ttgcagtggg cttgtctaat
     5341 atcaccaata atctcccctg ttgtgtaaaa ggccctgccc ggtccgatat gaatgctctt
     5401 gcgcgtgttg ttgtttgggc gagtacaatt gatttcgaca gactcgttca gctgaacaat
     5461 gatgggttta gcattatcgg cgaagttagc agatctgatc accacctctt cctctgccaa
     5521 ggatccattc agcagcagtt gtgtagacat aactgggcgg ataccgtgtg tgcactgtac
     5581 tgtactgaca tttgtacatg ggcctttgcc gttgaacttc ttgtccttgc atttcaggat
     5641 ggcaaagcct gctggggcgc agtagtggat tgggataggc tcgaagctca ccttggggca
     5701 ggcctgggta atcacagaag tgttgcagct aatcaggcgg tacctgttat tgctgttgtt
     5761 gtcgatagga gcgaggtcca gtttgtaaaa cagggcatac tctttctgaa ccttccctcg
     5821 gatattcgtc gttatattga atgagcagtt cttcatttct ccccctccca ccatccctcg
     5881 ggagcttgac gtggtatttg tgtcgtttcc gttcgtggca ttccggaggt cagtacaatt
     5941 cagcgttacg cacagatctg tcagtttcac gcagggttta agggactgat cccagaggct
     6001 aataatgtcc tcgtgcatct gctccaccat attatttttc cacatattga aattctcagt
     6061 cacgttcttc agttccactt cctgtgggtt ggggtcggta ggcacacagg cgtgtgtggc
     6121 ccacacatta tgcacctcgg tgtcgtatgc cttccggtcg gaggcgcaga agagcgtggt
     6181 atcagcctcc ttccaaactg ggaccccgta atacacagtc acccagagct tttcctcagc
     6241 cagcacagat gctaccaaca tgccgaggag atagagagtg gcgagcggtt gcaagctccc
     6301 catgggcatg gtggcgctag cagctggtac ccagcttcta gagatctgac ggttcactaa
     6361 acgagctctg cttatataga cctcccaccg tacacgccta ccgcccattt gcgtcaacgg
     6421 ggcggggtta ttacgacatt ttggaaagtc ccgttgattt tggtgccaaa acaaactccc
     6481 attgacgtca atggggtgga gacttggaaa tccccgtgag tcaaaccgct atccacgccc
     6541 attggtgtac tgccaaaacc gcatcaccat ggtaatagcg atgactaata cgtagatgta
     6601 ctgccaagta ggaaagtccc gtaaggtcat gtactgggca taatgccagg cgggccattt
     6661 accgtcattg acgtcaatag ggggcggact tggcatatga tacacttgat gtactgccaa
     6721 gtgggcagtt taccgtaaat actccaccca ttgacgtcaa tggaaagtcc ctattggcgt
     6781 tactatggga acatacgtca ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc
     6841 aggcgggcca tttaccgtaa gttatgtaac gcggaactcc atatatgggc tatgaactaa
     6901 tgaccccgta attgattact attaataact agtcaataat caatgtcaac atggcggtca
     6961 tattggacat gagccaatat aaatgtacat attatgatat agatacaacg tatgcaatgg
     7021 ccaatagcca atattgattt atgctatata accaatgact aatatggcta attgccaata
     7081 ttgattcaat gtatagatct tccataccta ccagttctgc gcctgcagca atggcaacaa
     7141 cgttgcccgg atctgcgatg ataagctgtc aaacatgaga attggtcgac tagcttggca
     7201 cgccagaaat ccgcgcggtg gtttttgggg gtcgggggtg tttggcagcc acagacgccc
     7261 ggtgttcgtg tcgcgccagt acatgcggtc catgcccagg ccatccaaaa accatgggtc
     7321 tgtctgctca gtccagtcgt ggacctgacc ccacgcaacg cccaaaataa taacccccac
     7381 gaaccataaa ccattcccca tgggggaccc cgtccctaac ccacggggcc agtggctatg
     7441 gcagggcctg ccgccccgac gttggctgcg agccctgggc cttcacccga acttgggggg
     7501 tggggtgggg aaaaggaaga aacgcgggcg tattggcccc aatggggtct cggtggggta
     7561 tcgacagagt gccagccctg ggaccgaacc ccgcgtttat gaacaaacga cccaacaccc
     7621 gtgcgtttta ttctgtcttt ttattgccgt catagcgcgg gttccttccg gtattgtctc
     7681 cttccgtgtt tcagttagcc tcccccatct cccgatccgg acgagtgctg gggcgtcggt
     7741 ttccactatc ggcgagtact tctacacagc catcggtcca gacggccgcg cttctgcggg
     7801 cgatttgtgt acgcccgaca gtcccggctc cggatcggac gattgcgtcg catcgaccct
     7861 gcgcccaagc tgcatcatcg aaattgccgt caaccaagct ctgatagagt tggtcaagac
     7921 caatgcggag catatacgcc cggagccgcg gcgatcctgc aagctccgga tgcctccgct
     7981 cgaagtagcg cgtctgctgc tccatacaag ccaaccacgg cctccagaag aagatgttgg
     8041 cgacctcgta ttgggaatcc ccgaacatcg cctcgctcca gtcaatgacc gctgttatgc
     8101 ggccattgtc cgtcaggaca ttgttggagc cgaaatccgc gtgcacgagg tgccggactt
     8161 cggggcagtc ctcggcccaa agcatcagct catcgagagc ctgcgcgacg gacgcactga
     8221 cggtgtcgtc catcacagtt tgccagtgat acacatgggg atcagcaatc gcgcatatga
     8281 aatcacgcca tgtagtgtat tgaccgattc cttgcggtcc gaatgggccg aacccgctcg
     8341 tctggctaag atcggccgca gcgatcgcat ccatggcctc cgcgaccggc tgcagaacag
     8401 cgggcagttc ggtttcaggc aggtcttgca acgtgacacc ctgtgcacgg cgggagatgc
     8461 aataggtcag gctctcgctg aattccccaa tgtcaagcac ttccggaatc gggagcgcgg
     8521 ccgatgcaaa gtgccgataa acataacgat ctttgtagaa accatcggcg cagctattta
     8581 cccgcaggac atatccacgc cctcctacat cgaagctgaa agcacgagat tcttcgccct
     8641 ccgagagctg catcaggtcg gagacgctgt cgaacttttc gatcagaaac ttctcgacag
     8701 acgtcgcggt gagttcaggc tttttcatat ctcattgccc cccgggatct gcggcacgct
     8761 gttgacgctg ttaagcgggt cgctgcaggg tcgctcggtg ttcgaggcca cacgcgtcac
     8821 cttaatatgc gaagtggacc tcggaccgcg ccgccccgac tgcatctgcg tgttcgaatt
     8881 cgccaatgac aagacgctgg gcggggtttg tgtcatcata gaactaaaga catgcaaata
     8941 tatttcttcc ggggacaccg ccagcaaacg cgagcaacgg gccacgggga tgaagcaggg
     9001 cggcacctcg ctaacggatt caccactcca agaattggag ccaatcaatt cttgcggaga
     9061 actgtgaatg cgcaaaccaa cccttggcag aacatatcca tcgcgtccgc catctccagc
     9121 agccgcacgc ggcgcatctc ggggccgacg cgctgggcta cgtcttgctg gcgttcgcga
     9181 cgcgaggctg gatggccttc cccattatga ttcttctcgc ttccggcggc atcgggatgc
     9241 ccgcgttgca ggccatgctg tccaggcagg tagatgacga ccatcaggga cagcttcaag
     9301 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     9361 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     9421 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     9481 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     9541 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     9601 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     9661 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     9721 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     9781 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     9841 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     9901 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     9961 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
    10021 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
    10081 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
    10141 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
    10201 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
    10261 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
    10321 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
    10381 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac
    10441 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
    10501 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
    10561 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
    10621 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
    10681 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aacacgggat aataccgcgc
    10741 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
    10801 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
    10861 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
    10921 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
    10981 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
    11041 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
    11101 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
    11161 ttcgtcttca agaattctca tgtttgacag cttatcatcg ataagctgat cctcacaggc
    11221 cgcacccagc ttttcttccg ttgccccagt agcatctctg tctggtgacc ttgaagagga
    11281 agaggagggg tcccgagaat ccccatccct accgtccagc aaaaaggggg acgaggaatt
    11341 tgaggcctgg cttgaggctc aggacgcaaa tcttgaggat gttcagcggg agttttccgg
    11401 gctgcgagta attggtgatg aggacgagga tggttcggag gatggggaat tttcagacct
    11461 ggatctgtct gacagcgacc atgaagggga tgaggg
//
