LOCUS       sequence-240352-       11820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the Q769.d22 HIV-1 
            isolate; QES mutant with enhanced binding to PG16.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11820)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 11820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-Q769-QES.i03.c04
FEATURES             Location/Qualifiers
     source          1..11820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(705..723)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        791..895
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(896..964,965..1756)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1114..1133)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1927..2515
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2416..2435
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2946..3091
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3134..4171
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4169..4188)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4213..4261
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4915..5294
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5295..5498
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5449..5469
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5495..5519
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     RBS             7183..7191
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    8137..8271
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8161..8180)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8215..8234
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(8414..8436)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8996..10785
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 tcctgttcca ccgtgggtcc ctttgcagcc aatgcaactt ggacgttttt ggggtctccg
       61 gacaccatct ctatgtcttg gccctgatcc tgagccgccc ggggctcctg gtcttccgcc
      121 tcctcgtcct cgtcctcttc cccgtcctcg tccatggtta tcaccccctc ttctttgagg
      181 tccactgccg ccggagcctt ctggtccaga tgtgtctccc ttctctccta ggccatttcc
      241 aggtcctgta cctggcccct cgtcagacat gattcacact aaaagagatc aatagacatc
      301 tttattagac gacgctcagt gaatacaggg agtgcagact cctgccccct ccaacagccc
      361 ccccaccctc atccccttca tggtcgctgt cagacagatc caggtctgaa aattccccat
      421 cctccgaacc atcctcgtcc tcatcaccaa ttactcgcag cccggaaaac tcccgctgaa
      481 catcctcaag atttgcgtcc tgagcctcaa gccaggcctc aaattcctcg tccccctttt
      541 tgctggacgg tagggatggg gattctcggg acccctcctc ttcctcttca aggtcaccag
      601 acagagatgc tactggggca acggaagaaa agctgggtgc ggcctgtgag gatcagctta
      661 tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct
      721 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
      781 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
      841 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
      901 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
      961 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     1021 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     1081 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt
     1141 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     1201 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     1261 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     1321 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     1381 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc
     1441 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     1501 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     1561 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     1621 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     1681 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     1741 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     1801 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     1861 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     1921 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     1981 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     2041 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     2101 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     2161 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     2221 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     2281 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     2341 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     2401 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     2461 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     2521 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttgaagct gtccctgatg
     2581 gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga
     2641 agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac
     2701 gtagcccagc gcgtcggccc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg
     2761 atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg
     2821 gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccctgcttc atccccgtgg
     2881 cccgttgctc gcgtttgctg gcggtgtccc cggaagaaat atatttgcat gtctttagtt
     2941 ctatgatgac acaaaccccg cccagcgtct tgtcattggc gaattcgaac acgcagatgc
     3001 agtcggggcg gcgcggtccg aggtccactt cgcatattaa ggtgacgcgt gtggcctcga
     3061 acaccgagcg accctgcagc gacccgctta acagcgtcaa cagcgtgccg cagatcccgg
     3121 ggggcaatga gatatgaaaa agcctgaact caccgcgacg tctgtcgaga agtttctgat
     3181 cgaaaagttc gacagcgtct ccgacctgat gcagctctcg gagggcgaag aatctcgtgc
     3241 tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg
     3301 tttctacaaa gatcgttatg tttatcggca ctttgcatcg gccgcgctcc cgattccgga
     3361 agtgcttgac attggggaat tcagcgagag cctgacctat tgcatctccc gccgtgcaca
     3421 gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc agccggtcgc
     3481 ggaggccatg gatgcgatcg ctgcggccga tcttagccag acgagcgggt tcggcccatt
     3541 cggaccgcaa ggaatcggtc aatacactac atggcgtgat ttcatatgcg cgattgctga
     3601 tccccatgtg tatcactggc aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca
     3661 ggctctcgat gagctgatgc tttgggccga ggactgcccc gaagtccggc acctcgtgca
     3721 cgcggatttc ggctccaaca atgtcctgac ggacaatggc cgcataacag cggtcattga
     3781 ctggagcgag gcgatgttcg gggattccca atacgaggtc gccaacatct tcttctggag
     3841 gccgtggttg gcttgtatgg agcagcagac gcgctacttc gagcggaggc atccggagct
     3901 tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc aactctatca
     3961 gagcttggtt gacggcaatt tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat
     4021 cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt
     4081 ctggaccgat ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc ccagcactcg
     4141 tccggatcgg gagatggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa
     4201 cccgcgctat gacggcaata aaaagacaga ataaaacgca cgggtgttgg gtcgtttgtt
     4261 cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat
     4321 tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa
     4381 ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagccac tggccccgtg
     4441 ggttagggac ggggtccccc atggggaatg gtttatggtt cgtgggggtt attattttgg
     4501 gcgttgcgtg gggtcaggtc cacgactgga ctgagcagac agacccatgg tttttggatg
     4561 gcctgggcat ggaccgcatg tactggcgcg acacgaacac cgggcgtctg tggctgccaa
     4621 acacccccga cccccaaaaa ccaccgcgcg gatttctggc gtgccaagct agtcgaccaa
     4681 ttctcatgtt tgacagctta tcatcgcaga tccgggcaac gttgttgcca ttgctgcagg
     4741 cgcagaactg gtaggtatgg aagatctata cattgaatca atattggcaa ttagccatat
     4801 tagtcattgg ttatatagca taaatcaata ttggctattg gccattgcat acgttgtatc
     4861 tatatcataa tatgtacatt tatattggct catgtccaat atgaccgcca tgttgacatt
     4921 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
     4981 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     5041 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     5101 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     5161 atcatatgcc aagtccgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     5221 atgcccagta catgacctta cgggactttc ctacttggca gtacatctac gtattagtca
     5281 tcgctattac catggtgatg cggttttggc agtacaccaa tgggcgtgga tagcggtttg
     5341 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     5401 aaaatcaacg ggactttcca aaatgtcgta ataaccccgc cccgttgacg caaatgggcg
     5461 gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatct
     5521 ctagaagctg ggtaccagct gctagcgcca ccatgcccat ggggtctttg caacccctcg
     5581 ctactcttta tttgctgggt atgttggtag cgagcgttct ggcggcgggc aatctctggg
     5641 taaccgtgta ctacggcgtg ccagtgtggc gggatgctga aacaaccctc ttttgtgcaa
     5701 gtgatgcaaa agcgtatgac cgagaagcac acaatgtttg ggctacacat gcgtgcgttc
     5761 cgacagatcc atccccccaa gaagtaccat tggggaatgt aaccgaggaa ttcaacatgt
     5821 ggaagaataa catggttgaa cagatgcata cagacatcat ctctttgtgg gaccagtcac
     5881 tgcagccgtg tgtgaaactt acgcctcttt gtgtcacgct gaattgttcc aatagtaata
     5941 acattccaag tgtcagcaac attaccgatg atatgaaaga agagattaag aactgcagct
     6001 ttaacatgac aaccgaattg aaggataaaa agcagaacgt ttactctttg ttctatcgac
     6061 tggacgttgt acccctcgaa acaaaaacca accagaacag tagtcactca aggtatcggc
     6121 tcattaactg caacacgtct gagatcacgc aggcttgccc aaaagtttct ttcgagccga
     6181 tccctattca ctattgtgcg cctgcaggct atgctatcct gaaatgtaac gacaagggct
     6241 ttaacggcac tggactttgt aaaaacgtct caacagtaca atgcacgcat gggattaaac
     6301 cggtcatgag tacccagctg ctccttaatg gaagccttgc cgagggtaag gtaatggtac
     6361 gatccgaaaa tattacaaat aatgcgaaaa acattataat acagtttaac aactctgtgc
     6421 aaattaattg cacgcgccca ggtaataaca cgcgaaaaag tatacatctc gggcctggga
     6481 aagtttttta cgcgacggac ataatagggg atataaggaa agcgcactgt aatgttaatc
     6541 gccaacaatg gaataagacc cttcaggatg tggcaacgca actcaggacc cacttcagaa
     6601 accgtacgat catatttaat aatagtctcg gtggggattt ggaaataact acgcatagtt
     6661 ttaattgtag gggagagtgg ttctattgta acacttccgg gctctttaat gggatatgga
     6721 acggaacgca agaacctaat cggaccgagt ctaacgatac aattacgctt caatgtcgga
     6781 taaagcagat catcaacatg tggcagagag taggccaggc aatttacgca ccccctatcc
     6841 agggggagat cagatgcgaa agtaacataa ccggacttat cctgacgagg gatgggggga
     6901 tcatcaacag tacagaggaa acgtttagac ctgggggcgg ggacatgaga gataattgga
     6961 ggtccgagct ctataaatac aaagttgtaa agatagaacc gcttggagtg gctccaacta
     7021 aggccaagag acgggtcgtg gagagagaaa aacgggcggt aggatttggc gcgttctttc
     7081 tcggtttcct tggcgcagcc ggatcaacga tgggggccgc ctccatcacc ctgacggttc
     7141 aggcgcgcca gcttttgagt gggattgtgc agcagcagaa taacctcctt agggccatag
     7201 aggctcagca gcacctgctg aagcttaccg tttggggcat taagcaactg caggcgcggg
     7261 tactcgcggt tgaaagatac cttaaggatc agcaactgct gggaatgtgg ggatgtagtg
     7321 gaaagttcat ctgcacaacc acggtcccat ggaattcctc atggtcaaat aaatcacagt
     7381 cagaaatatg ggataatatg acgtggatgc agtgggacaa ggagattaac aattacaccc
     7441 agattatcta cgatcttatt gaggagtcac aaaggcaaca agagaaaaac gaacaggact
     7501 tgctggcgct cgacaaatgg gctaatttgt ggaactggtt cgacatcagt aactggctgt
     7561 ggtacataaa gattttcata atgatagttg gcgggctcat agggcttcgg attgcgttcg
     7621 cagtgctttc tgtcatcaat agggtcaggc aaggttattc cccgctctcc tttcaaaccc
     7681 ataccccaaa ccctcgggat ttggataggc caggccggat agaggaggaa ggaggtgagc
     7741 aggatcggga ccgaagcata agactggtct ctgggtttct tgctttggca tgggacgacc
     7801 tgaggtctct gtgtttgttc agttaccacc gcctgcgaga tttcatcttg gtggctgcta
     7861 gaacggtcga acttcttgga catatttcac ttaaaggctt gagacgaggc tgggaaggat
     7921 tgaaatacct tgggaacctt ctgtcatact ggggacggga actgaagata agtgccataa
     7981 acttgttgga cacaatcgcg attgttgtgg cggaatggac cgatcggata attgagattg
     8041 gccagagact gtgtcgcgct atcattaata tacctcggag aatccgacag gggttcgaac
     8101 gggctttgtt gtagtaactc gaggccggca aggccggatc cagacatgat aagatacatt
     8161 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt
     8221 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac
     8281 aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag
     8341 taaaacctct acaaatgtgg tatggctgat tatgatccgg ctgcctcgcg cgtttcggtg
     8401 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
     8461 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     8521 gcgcagccat gaggtcgact ctagaggatc gatccccgcc ccggacgaac taaacctgac
     8581 tacgacatct ctgccccttc ttcgcggggc agtgcatgta atcccttcag ttggttggta
     8641 caacttgcca actgggccct gttccacatg tgacacgggg ggggaccaaa cacaaagggg
     8701 ttctctgact gtagttgaca tccttataaa tggatgtgca catttgccaa cactgagtgg
     8761 ctttcatcct ggagcagact ttgcagtctg tggactgcaa cacaacattg cctttatgtg
     8821 taactcttgg ctgaagctct tacaccaatg ctgggggaca tgtacctccc aggggcccag
     8881 gaagactacg ggaggctaca ccaacgtcaa tcagaggggc ctgtgtagct accgataagc
     8941 ggaccctcaa gagggcatta gcaatagtgt ttataaggcc cccttgttaa ccctaaacgg
     9001 gtagcatatg cttcccgggt agtagtatat actatccaga ctaaccctaa ttcaatagca
     9061 tatgttaccc aacgggaagc atatgctatc gaattagggt tagtaaaagg gtcctaagga
     9121 acagcgatat ctcccacccc atgagctgtc acggttttat ttacatgggg tcaggattcc
     9181 acgagggtag tgaaccattt tagtcacaag ggcagtggct gaagatcaag gagcgggcag
     9241 tgaactctcc tgaatcttcg cctgcttctt cattctcctt cgtttagcta atagaataac
     9301 tgctgagttg tgaacagtaa ggtgtatgtg aggtgctcga aaacaaggtt tcaggtgacg
     9361 cccccagaat aaaatttgga cggggggttc agtggtggca ttgtgctatg acaccaatat
     9421 aaccctcaca aaccccttgg gcaataaata ctagtgtagg aatgaaacat tctgaatatc
     9481 tttaacaata gaaatccatg gggtggggac aagccgtaaa gactggatgt ccatctcaca
     9541 cgaatttatg gctatgggca acacataatc ctagtgcaat atgatactgg ggttattaag
     9601 atgtgtccca ggcagggacc aagacaggtg aaccatgttg ttacactcta tttgtaacaa
     9661 ggggaaagag agtggacgcc gacagcagcg gactccactg gttgtctcta acacccccga
     9721 aaattaaacg gggctccacg ccaatggggc ccataaacaa agacaagtgg ccactctttt
     9781 ttttgaaatt gtggagtggg ggcacgcgtc agcccccaca cgccgccctg cggttttgga
     9841 ctgtaaaata agggtgtaat aacttggctg attgtaaccc cgctaaccac tgcggtcaaa
     9901 ccacttgccc acaaaaccac taatggcacc ccggggaata cctgcataag taggtgggcg
     9961 ggccaagata ggggcgcgat tgctgcgatc tggaggacaa attacacaca cttgcgcctg
    10021 agcgccaagc acagggttgt tggtcctcat attcacgagg tcgctgagag cacggtgggc
    10081 taatgttgcc atgggtagca tatactaccc aaatatctgg atagcatatg ctatcctaat
    10141 ctatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat
    10201 ctatatctgg gtagtatatg ctatcctaat ttatatctgg gtagcatagg ctatcctaat
    10261 ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat
    10321 ctgtatccgg gtagcatatg ctatcctaat agagattagg gtagtatatg ctatcctaat
    10381 ttatatctgg gtagcatata ctacccaaat atctggatag catatgctat cctaatctat
    10441 atctgggtag catatgctat cctaatctat atctgggtag cataggctat cctaatctat
    10501 atctgggtag catatgctat cctaatctat atctgggtag tatatgctat cctaatttat
    10561 atctgggtag cataggctat cctaatctat atctgggtag catatgctat cctaatctat
    10621 atctgggtag tatatgctat cctaatctgt atccgggtag catatgctat cctcatgcat
    10681 atacagtcag catatgatac ccagtagtag agtgggagtg ctatcctttg catatgccgc
    10741 cacctcccaa gggggcgtga attttcgctg cttgtccttt tcctgctggt tgctcccatt
    10801 cttaggtgaa tttaaggagg ccaggctaaa gccgtcgcat gtctgattgc tcaccaggta
    10861 aatgtcgcta atgttttcca acgcgagaag gtgttgagcg cggagctgag tgacgtgaca
    10921 acatgggtat gcccaattgc cccatgttgg gaggacgaaa atggtgacaa gacagatggc
    10981 cagaaataca ccaacagcac gcatgatgtc tactggggat ttattcttta gtgcggggga
    11041 atacacggct tttaatacga ttgagggcgt ctcctaacaa gttacatcac tcctgccctt
    11101 cctcaccctc atctccatca cctccttcat ctccgtcatc tccgtcatca ccctccgcgg
    11161 cagccccttc caccataggt ggaaaccagg gaggcaaatc tactccatcg tcaaagctgc
    11221 acacagtcac cctgatattg caggtaggag cgggctttgt cataacaagg tccttaatcg
    11281 catccttcaa aacctcagca aatatatgag tttgtaaaaa gaccatgaaa taacagacaa
    11341 tggactccct tagcgggcca ggttgtgggc cgggtccagg ggccattcca aaggggagac
    11401 gactcaatgg tgtaagacga cattgtggaa tagcaagggc agttcctcgc cttaggttgt
    11461 aaagggaggt cttactacct ccatatacga acacaccggc gacccaagtt ccttcgtcgg
    11521 tagtcctttc tacgtgactc ctagccagga gagctcttaa accttctgca atgttctcaa
    11581 atttcgggtt ggaacctcct tgaccacgat gctttccaaa ccaccctcct tttttgcgcc
    11641 tgcctccatc accctgaccc cggggtccag tgcttgggcc ttctcctggg tcatctgcgg
    11701 ggccctgctc tatcgctccc gggggcacgt caggctcacc atctgggcca ccttcttggt
    11761 ggtattcaaa ataatcggct tcccctacag ggtggaaaaa tggccttcta cctggagggg
//
