LOCUS       sequence-240362-        8475 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Produces equimolar expression of mTq2 and mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8475)
  AUTHORS   Botman D, de Groot DH, Schmidt P, Goedhart J, Teusink B
  TITLE     In vivo characterisation of fluorescent proteins in budding yeast.
  JOURNAL   Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.1038/s41598-019-38913-z.
  PUBMED    30783202
REFERENCE   2  (bases 1 to 8475)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2019-02-19"; volume: "9(1):2234. doi"; pages: " 
            10.1038/s41598-019-38913-z"
COMMENT     Sequence Label: pDRF1-GW mTurquoise2-T2A-mcherry
FEATURES             Location/Qualifiers
     source          1..8475
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    125..149
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      166..175
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(172..174,175..177,178..888)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(217..238)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(478..497)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     825..846
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             895..948
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      964..973
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             970..1680
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1115..1133)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1383..1402
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(1690..1714)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(1776..1795)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1777..1795)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      1943..2130
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(1998..2022)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(2142..2159)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2142..2158)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2151..2173)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2594..3940
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(4051..4854)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(4855..5070)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(5095..5113)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5181..5285
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5286..5354,5355..6146)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5504..5523)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6317..6905
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6806..6825
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7059..7076
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7193..7214
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7229..7234,7235..7252,7253..7259)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7267..7283
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7272..7294
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7291..7307
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7291..7307
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(7337..7792)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7474..7495)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7686..7705
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tttctttaac aatcgttaat aattaattaa ttggaaaata accatttttt ctctctttta
       61 tacacacatt caaaagaaag aaaaaaaata taccccagcc tcgactagtg aattcgcggc
      121 catcacaagt ttgtacaaaa aagcaggctg ctagcgctac cggtcgccac catggtgagc
      181 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
      241 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
      301 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
      361 accctgtcct ggggcgtgca gtgcttcgcc cgctaccccg accacatgaa gcagcacgac
      421 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
      481 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
      541 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
      601 tacaactact ttagcgacaa cgtctatatc accgccgaca agcagaagaa cggcatcaag
      661 gccaacttca agatccgcca caacatcgag gacggcggcg tgcagctcgc cgaccactac
      721 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
      781 acccagtcca agctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
      841 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagtc cggagagggc
      901 agaggaagtc ttctaacatg cggtgacgtg gaggagaatc ccggccctgg ggatccaccg
      961 gtcgccacca tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg
     1021 cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag
     1081 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc
     1141 cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac
     1201 gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag
     1261 tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc
     1321 ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac
     1381 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc
     1441 gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac
     1501 tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc
     1561 tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa
     1621 cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagtaa
     1681 agcggccgca cccagctttc ttgtacaaag tggtgtagtt aattcatgat ggccgctgca
     1741 ggtcgacctc gagggggggc ccggtaccca attcgcccta tagtgagtcg tattacgcgc
     1801 ggatccagct ttggacttct tcgccagagg tttggtcaag tctccaatca aggttgtcgg
     1861 cttgtctacc ttgccagaaa tttacgaaaa gatggaaaag ggtcaaatcg ttggtagata
     1921 cgttgttgac acttctaaat aagcgaattt cttatgattt atgattttta ttattaaata
     1981 agttataaaa aaaataagtg tatacaaatt ttaaagtgac tcttaggttt taaaacgaaa
     2041 attcttattc ttgagtaact ctttcctgta ggtcaggttg ctttctcagg tatagcatga
     2101 ggtcgctctt attgaccaca cctctaccgg catgccaatt cactggccgt cgttttacaa
     2161 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     2221 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     2281 agcctgaatg gcgaatggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     2341 tcacaccgca taatcggatc gtacttgtta cccatcattg aattttgaac atccgaacct
     2401 ggaagttttc cctgaaacag atagtatatt tgaacctgta taataatata tagtctagcg
     2461 ctttacggaa gacaatgtat gtatttcggt tcctggagaa actattgcat ctattgcata
     2521 ggtaatcttg cacgtcgcat ccccggttca ttttctgcgt ttccatcttg cacttcaata
     2581 gcatatcttt gttaacgaag catctgtgct tcattttgta gaacaaaaat gcaacgcgag
     2641 agcgctaatt tttcaaacaa agaatctgag ctgcattttt acagaacaga aatgcaacgc
     2701 gaaagcgcta ttttaccaac gaagaatctg tgcttcattt ttgtaaaaca aaaatgcaac
     2761 gcgagagcgc taatttttca aacaaagaat ctgagctgca tttttacaga acagaaatgc
     2821 aacgcgagag cgctatttta ccaacaaaga atctatactt cttttttgtt ctacaaaaat
     2881 gcatcccgag agcgctattt ttctaacaaa gcatcttaga ttactttttt tctcctttgt
     2941 gcgctctata atgcagtctc ttgataactt tttgcactgt aggtccgtta aggttagaag
     3001 aaggctactt tggtgtctat tttctcttcc ataaaaaaag cctgactcca cttcccgcgt
     3061 ttactgatta ctagcgaagc tgcgggtgca ttttttcaag ataaaggcat ccccgattat
     3121 attctatacc gatgtggatt gcgcatactt tgtgaacaga aagtgatagc gttgatgatt
     3181 cttcattggt cagaaaatta tgaacggttt cttctatttt gtctctatat actacgtata
     3241 ggaaatgttt acattttcgt attgttttcg attcactcta tgaatagttc ttactacaat
     3301 ttttttgtct aaagagtaat actagagata aacataaaaa atgtagaggt cgagtttaga
     3361 tgcaagttca aggagcgaaa ggtggatggg taggttatat agggatatag cacagagata
     3421 tatagcaaag agatactttt gagcaatgtt tgtggaagcg gtattcgcaa tattttagta
     3481 gctcgttaca gtccggtgcg tttttggttt tttgaaagtg cgtcttcaga gcgcttttgg
     3541 ttttcaaaag cgctctgaag ttcctatact ttctagctag agaataggaa cttcggaata
     3601 ggaacttcaa agcgtttccg aaaacgagcg cttccgaaaa tgcaacgcga gctgcgcaca
     3661 tacagctcac tgttcacgtc gcacctatat ctgcgtgttg cctgtatata tatatacatg
     3721 agaagaacgg catagtgcgt gtttatgctt aaatgcgtac ttatatgcgt ctatttatgt
     3781 aggatgaaag gtagtctagt acctcctgtg atattatccc attccatgcg gggtatcgta
     3841 tgcttccttc agcactaccc tttagctgtt ctatatgctg ccactcctca attggattag
     3901 tctcatcctt caatgctatc atttcctttg atattggatc gatccgatga taagctgtca
     3961 aacatgagaa ttgggtaata actgatataa ttaaattgaa gctctaattt gtgagtttag
     4021 tatacatgca tttacttata atacagtttt ttagttttgc tggccgcatc ttctcaaata
     4081 tgcttcccag cctgcttttc tgtaacgttc accctctacc ttagcatccc ttccctttgc
     4141 aaatagtcct cttccaacaa taataatgtc agatcctgta gagaccacat catccacggt
     4201 tctatactgt tgacccaatg cgtctccctt gtcatctaaa cccacaccgg gtgtcataat
     4261 caaccaatcg taaccttcat ctcttccacc catgtctctt tgagcaataa agccgataac
     4321 aaaatctttg tcgctcttcg caatgtcaac agtaccctta gtatattctc cagtagatag
     4381 ggagcccttg catgacaatt ctgctaacat caaaaggcct ctaggttcct ttgttacttc
     4441 ttctgccgcc tgcttcaaac cgctaacaat acctgggccc accacaccgt gtgcattcgt
     4501 aatgtctgcc cattctgcta ttctgtatac acccgcagag tactgcaatt tgactgtatt
     4561 accaatgtca gcaaattttc tgtcttcgaa gagtaaaaaa ttgtacttgg cggataatgc
     4621 ctttagcggc ttaactgtgc cctccatgga aaaatcagtc aagatatcca catgtgtttt
     4681 tagtaaacaa attttgggac ctaatgcttc aactaactcc agtaattcct tggtggtacg
     4741 aacatccaat gaagcacaca agtttgtttg cttttcgtgc atgatattaa atagcttggc
     4801 agcaacagga ctaggatgag tagcagcacg ttccttatat gtagctttcg acatgattta
     4861 tcttcgtttc ctgcatgttt ttgttctgtg cagttgggtt aagaatactg ggcaatttca
     4921 tgtttcttca acactacata tgcgtatata taccaatcta agtctgtgct ccttccttcg
     4981 ttcttccttc tgttcggaga ttaccgaatc aaaaaaattt caaggaaacc gaaatcaaaa
     5041 aaaagaataa aaaaaaaatg atgaattgaa aagctaattc ttgaagacga aagggcctcg
     5101 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     5161 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     5221 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     5281 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     5341 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     5401 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     5461 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     5521 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     5581 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     5641 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     5701 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     5761 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     5821 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     5881 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     5941 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     6001 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     6061 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     6121 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     6181 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     6241 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     6301 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     6361 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     6421 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     6481 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     6541 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     6601 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     6661 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     6721 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     6781 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     6841 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     6901 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     6961 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     7021 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     7081 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     7141 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     7201 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     7261 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     7321 agcttaccgc atcaggaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt
     7381 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca
     7441 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
     7501 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta
     7561 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
     7621 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
     7681 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg
     7741 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtccattcgc
     7801 caagcttcct gaaacggaga aacataaaca ggcattgctg ggatcaccca tacatcactc
     7861 tgttttgcct gaccttttcc ggtaatttga aaacaaaccc ggtctcgaag cggagatccg
     7921 gcgataatta ccgcagaaat aaacccatac acgagacgta gaaccagccg cacatggccg
     7981 gagaaactcc tgcgagaatt tcgtaaactc gcgcgcattg catctgtatt tcctaatgcg
     8041 gcacttccag gcctcgatcg agaccgttta tccattgctt ttttgttgtc tttttccctc
     8101 gttcacagaa agtctgaaga agctatagta gaactatgag ctttttttgt ttctgttttc
     8161 cttttttttt tttttacctc tgtggaaatt gttactctca cactctttag ttcgtttgtt
     8221 tgttttgttt attccaatta tgaccggtga cgaaacgtgg tcgatggtgg gtaccgctta
     8281 tgctcccctc cattagtttc gattatataa aaaggccaaa tattgtatta ttttcaaatg
     8341 tcctatcatt atcgtctaac atctaatttc tcttaaattt tttctctttc tttcctataa
     8401 caccaatagt gaaaatcttt ttttcttcta tatctacaaa aacttttttt ttctatcaac
     8461 ctcgttgata aattt
//
