LOCUS       sequence-240366-        8469 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Produces equimolar expression of yeVenus and mTq2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8469)
  AUTHORS   Botman D, de Groot DH, Schmidt P, Goedhart J, Teusink B
  TITLE     In vivo characterisation of fluorescent proteins in budding yeast.
  JOURNAL   Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.1038/s41598-019-38913-z.
  PUBMED    30783202
REFERENCE   2  (bases 1 to 8469)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2019-02-19"; volume: "9(1):2234. doi"; pages: " 
            10.1038/s41598-019-38913-z"
COMMENT     Sequence Label: pDRF1-GW yeVenus-T2A-mTurquoise2
FEATURES             Location/Qualifiers
     source          1..8469
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    145..169
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             180..893
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             900..953
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      969..978
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(975..977,978..980,981..1694)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1020..1041)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1281..1300)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1628..1649
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(1704..1728)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(1790..1809)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1791..1809)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      1957..2144
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2012..2036)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(2156..2173)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2156..2172)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2165..2187)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2608..3954
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(4065..4868)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(4869..5084)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(5109..5127)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5195..5299
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5300..5368,5369..6160)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5518..5537)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6331..6919
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6820..6839
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7073..7090
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7207..7228
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7243..7248,7249..7266,7267..7273)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7281..7297
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7286..7308
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7305..7321
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7305..7321
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(7351..7806)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7488..7509)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7700..7719
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tcaacctcgt tgataaattt tttctttaac aatcgttaat aattaattaa ttggaaaata
       61 accatttttt ctctctttta tacacacatt caaaagaaag aaaaaaaata taccccagcc
      121 tcgactagtg aattcgcggc catcacaagt ttgtacaaaa aagcaggctg ctagccacca
      181 tgtctaaagg tgaagaatta ttcactggtg ttgtcccaat tttggttgaa ttagatggtg
      241 atgttaatgg tcacaaattt tctgtctccg gtgaaggtga aggtgatgct acttacggta
      301 aattgacctt aaaattgatt tgtactactg gtaaattgcc agttccatgg ccaaccttag
      361 tcactacttt aggttatggt ttgcaatgtt ttgctagata cccagatcat atgaaacaac
      421 atgacttttt caagtctgcc atgccagaag gttatgttca agaaagaact atttttttca
      481 aagatgacgg taactacaag accagagctg aagtcaagtt tgaaggtgat accttagtta
      541 atagaatcga attaaaaggt attgatttta aagaagatgg taacatttta ggtcacaaat
      601 tggaatacaa ctataactct cacaatgttt acatcactgc tgacaaacaa aagaatggta
      661 tcaaagctaa cttcaaaatt agacacaaca ttgaagatgg tggtgttcaa ttagctgacc
      721 attatcaaca aaatactcca attggtgatg gtccagtctt gttaccagac aaccattact
      781 tatcctatca atctgcctta tccaaagatc caaacgaaaa gagagaccac atggtcttgt
      841 tagaatttgt tactgctgct ggtattaccc atggtatgga tgaattgtac aaatccggag
      901 agggcagagg aagtcttcta acatgcggtg acgtggagga gaatcccggc cctggggatc
      961 caccggtcgc caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     1021 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     1081 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     1141 tgccctggcc caccctcgtg accaccctgt cctggggcgt gcagtgcttc gcccgctacc
     1201 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     1261 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     1321 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     1381 acatcctggg gcacaagctg gagtacaact actttagcga caacgtctat atcaccgccg
     1441 acaagcagaa gaacggcatc aaggccaact tcaagatccg ccacaacatc gaggacggcg
     1501 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     1561 tgcccgacaa ccactacctg agcacccagt ccaagctgag caaagacccc aacgagaagc
     1621 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     1681 agctgtacaa gtaaagcggc cgcacccagc tttcttgtac aaagtggtgt agttaattca
     1741 tgatggccgc tgcaggtcga cctcgagggg gggcccggta cccaattcgc cctatagtga
     1801 gtcgtattac gcgcggatcc agctttggac ttcttcgcca gaggtttggt caagtctcca
     1861 atcaaggttg tcggcttgtc taccttgcca gaaatttacg aaaagatgga aaagggtcaa
     1921 atcgttggta gatacgttgt tgacacttct aaataagcga atttcttatg atttatgatt
     1981 tttattatta aataagttat aaaaaaaata agtgtataca aattttaaag tgactcttag
     2041 gttttaaaac gaaaattctt attcttgagt aactctttcc tgtaggtcag gttgctttct
     2101 caggtatagc atgaggtcgc tcttattgac cacacctcta ccggcatgcc aattcactgg
     2161 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     2221 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     2281 cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt ctccttacgc
     2341 atctgtgcgg tatttcacac cgcataatcg gatcgtactt gttacccatc attgaatttt
     2401 gaacatccga acctgggagt tttccctgaa acagatagta tatttgaacc tgtataataa
     2461 tatatagtct agcgctttac ggaagacaat gtatgtattt cggttcctgg agaaactatt
     2521 gcatctattg cataggtaat cttgcacgtc gcatccccgg ttcattttct gcgtttccat
     2581 cttgcacttc aatagcatat ctttgttaac gaagcatctg tgcttcattt tgtagaacaa
     2641 aaatgcaacg cgagagcgct aatttttcaa acaaagaatc tgagctgcat ttttacagaa
     2701 cagaaatgca acgcgaaagc gctattttac caacgaagaa tctgtgcttc atttttgtaa
     2761 aacaaaaatg caacgcgaga gcgctaattt ttcaaacaaa gaatctgagc tgcattttta
     2821 cagaacagaa atgcaacgcg agagcgctat tttaccaaca aagaatctat acttcttttt
     2881 tgttctacaa aaatgcatcc cgagagcgct atttttctaa caaagcatct tagattactt
     2941 tttttctcct ttgtgcgctc tataatgcag tctcttgata actttttgca ctgtaggtcc
     3001 gttaaggtta gaagaaggct actttggtgt ctattttctc ttccataaaa aaagcctgac
     3061 tccacttccc gcgtttactg attactagcg aagctgcggg tgcatttttt caagataaag
     3121 gcatccccga ttatattcta taccgatgtg gattgcgcat actttgtgaa cagaaagtga
     3181 tagcgttgat gattcttcat tggtcagaaa attatgaacg gtttcttcta ttttgtctct
     3241 atatactacg tataggaaat gtttacattt tcgtattgtt ttcgattcac tctatgaata
     3301 gttcttacta caattttttt gtctaaagag taatactaga gataaacata aaaaatgtag
     3361 aggtcgagtt tagatgcaag ttcaaggagc gaaaggtgga tgggtaggtt atatagggat
     3421 atagcacaga gatatatagc aaagagatac ttttgagcaa tgtttgtgga agcggtattc
     3481 gcaatatttt agtagctcgt tacagtccgg tgcgtttttg gttttttgaa agtgcgtctt
     3541 cagagcgctt ttggttttca aaagcgctct gaagttccta tactttctag ctagagaata
     3601 ggaacttcgg aataggaact tcaaagcgtt tccgaaaacg agcgcttccg aaaatgcaac
     3661 gcgagctgcg cacatacagc tcactgttca cgtcgcacct atatctgcgt gttgcctgta
     3721 tatatatata catgagaaga acggcatagt gcgtgtttat gcttaaatgc gtacttatat
     3781 gcgtctattt atgtaggatg aaaggtagtc tagtacctcc tgtgatatta tcccattcca
     3841 tgcggggtat cgtatgcttc cttcagcact accctttagc tgttctatat gctgccactc
     3901 ctcaattgga ttagtctcat ccttcaatgc tatcatttcc tttgatattg gatcgatccg
     3961 atgataagct gtcaaacatg agaattgggt aataactgat ataattaaat tgaagctcta
     4021 atttgtgagt ttagtataca tgcatttact tataatacag ttttttagtt ttgctggccg
     4081 catcttctca aatatgcttc ccagcctgct tttctgtaac gttcaccctc taccttagca
     4141 tcccttccct ttgcaaatag tcctcttcca acaataataa tgtcagatcc tgtagagacc
     4201 acatcatcca cggttctata ctgttgaccc aatgcgtctc ccttgtcatc taaacccaca
     4261 ccgggtgtca taatcaacca atcgtaacct tcatctcttc cacccatgtc tctttgagca
     4321 ataaagccga taacaaaatc tttgtcgctc ttcgcaatgt caacagtacc cttagtatat
     4381 tctccagtag atagggagcc cttgcatgac aattctgcta acatcaaaag gcctctaggt
     4441 tcctttgtta cttcttctgc cgcctgcttc aaaccgctaa caatacctgg gcccaccaca
     4501 ccgtgtgcat tcgtaatgtc tgcccattct gctattctgt atacacccgc agagtactgc
     4561 aatttgactg tattaccaat gtcagcaaat tttctgtctt cgaagagtaa aaaattgtac
     4621 ttggcggata atgcctttag cggcttaact gtgccctcca tggaaaaatc agtcaagata
     4681 tccacatgtg tttttagtaa acaaattttg ggacctaatg cttcaactaa ctccagtaat
     4741 tccttggtgg tacgaacatc caatgaagca cacaagtttg tttgcttttc gtgcatgata
     4801 ttaaatagct tggcagcaac aggactagga tgagtagcag cacgttcctt atatgtagct
     4861 ttcgacatga tttatcttcg tttcctgcat gtttttgttc tgtgcagttg ggttaagaat
     4921 actgggcaat ttcatgtttc ttcaacacta catatgcgta tatataccaa tctaagtctg
     4981 tgctccttcc ttcgttcttc cttctgttcg gagattaccg aatcaaaaaa atttcaagga
     5041 aaccgaaatc aaaaaaaaga ataaaaaaaa aatgatgaat tgaaaagcta attcttgaag
     5101 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     5161 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     5221 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     5281 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     5341 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     5401 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     5461 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     5521 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     5581 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     5641 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     5701 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     5761 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     5821 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     5881 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     5941 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     6001 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     6061 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     6121 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     6181 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     6241 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     6301 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     6361 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     6421 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     6481 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     6541 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     6601 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     6661 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     6721 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     6781 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     6841 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     6901 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     6961 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     7021 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     7081 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     7141 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     7201 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     7261 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     7321 ccatgattac gccaagctta ccgcatcagg aaattgtaag cgttaatatt ttgttaaaat
     7381 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
     7441 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     7501 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     7561 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
     7621 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     7681 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
     7741 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
     7801 gcgcgtccat tcgccaagct tcctgaaacg gagaaacata aacaggcatt gctgggatca
     7861 cccatacatc actctgtttt gcctgacctt ttccggtaat ttgaaaacaa acccggtctc
     7921 gaagcggaga tccggcgata attaccgcag aaataaaccc atacacgaga cgtagaacca
     7981 gccgcacatg gccggagaaa ctcctgcgag aatttcgtaa actcgcgcgc attgcatctg
     8041 tatttcctaa tgcggcactt ccaggcctcg atcgagaccg tttatccatt gcttttttgt
     8101 tgtctttttc cctcgttcac agaaagtctg aagaagctat agtagaacta tgagcttttt
     8161 ttgtttctgt tttccttttt ttttttttta cctctgtgga aattgttact ctcacactct
     8221 ttagttcgtt tgtttgtttt gtttattcca attatgaccg gtgacgaaac gtggtcgatg
     8281 gtgggtaccg cttatgctcc cctccattag tttcgattat ataaaaaggc caaatattgt
     8341 attattttca aatgtcctat cattatcgtc taacatctaa tttctcttaa attttttctc
     8401 tttctttcct ataacaccaa tagtgaaaat ctttttttct tctatatcta caaaaacttt
     8461 ttttttcta
//
