LOCUS       sequence-240369-       11322 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the ZM197M HIV-1 isolate; 
            QES mutant with enhanced binding to PG16.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11322)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 11322)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-ZM197M-QES.c10
FEATURES             Location/Qualifiers
     source          1..11322
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        48..152
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(153..221,222..1013)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(371..390)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1184..1772
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1673..1692
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2203..2348
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             2391..3428
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(3426..3445)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    3470..3518
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4172..4551
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4552..4755
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4706..4726
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4752..4776
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     polyA_signal    7451..7585
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7475..7494)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7529..7548
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(7728..7750)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8310..10099
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
       61 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
      121 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
      181 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
      241 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
      301 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
      361 ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg
      421 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
      481 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
      541 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
      601 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
      661 ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca
      721 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
      781 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
      841 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
      901 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
      961 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     1021 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     1081 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     1141 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     1201 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     1261 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     1321 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     1381 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     1441 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     1501 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     1561 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     1621 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     1681 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     1741 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     1801 tcctggcctt ttgctggcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca
     1861 gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga
     1921 aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccccga
     1981 gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt
     2041 ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa
     2101 tccgttagcg aggtgccgcc ctgcttcatc cccgtggccc gttgctcgcg tttgctggcg
     2161 gtgtccccgg aagaaatata tttgcatgtc tttagttcta tgatgacaca aaccccgccc
     2221 agcgtcttgt cattggcgaa ttcgaacacg cagatgcagt cggggcggcg cggtccgagg
     2281 tccacttcgc atattaaggt gacgcgtgtg gcctcgaaca ccgagcgacc ctgcagcgac
     2341 ccgcttaaca gcgtcaacag cgtgccgcag atcccggggg gcaatgagat atgaaaaagc
     2401 ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtctccg
     2461 acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc
     2521 gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat cgttatgttt
     2581 atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt ggggaattca
     2641 gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg caagacctgc
     2701 ctgaaaccga actgcccgct gttctgcagc cggtcgcgga ggccatggat gcgatcgctg
     2761 cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga atcggtcaat
     2821 acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa
     2881 ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt
     2941 gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg
     3001 tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg atgttcgggg
     3061 attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct tgtatggagc
     3121 agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgccg cggctccggg
     3181 cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac ggcaatttcg
     3241 atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg
     3301 tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag
     3361 tactcgccga tagtggaaac cgacgcccca gcactcgtcc ggatcgggag atgggggagg
     3421 ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa
     3481 agacagaata aaacgcacgg gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca
     3541 gggctggcac tctgtcgata ccccaccgag accccattgg ggccaatacg cccgcgtttc
     3601 ttccttttcc ccaccccacc ccccaagttc gggtgaaggc ccagggctcg cagccaacgt
     3661 cggggcggca ggccctgcca tagccactgg ccccgtgggt tagggacggg gtcccccatg
     3721 gggaatggtt tatggttcgt gggggttatt attttgggcg ttgcgtgggg tcaggtccac
     3781 gactggactg agcagacaga cccatggttt ttggatggcc tgggcatgga ccgcatgtac
     3841 tggcgcgaca cgaacaccgg gcgtctgtgg ctgccaaaca cccccgaccc ccaaaaacca
     3901 ccgcgcggat ttctggcgtg ccaagctagt cgaccaattc tcatgtttga cagcttatca
     3961 tcgcagatcc gggcaacgtt gttgccattg ctgcaggcgc agaactggta ggtatggaag
     4021 atctatacat tgaatcaata ttggcaatta gccatattag tcattggtta tatagcataa
     4081 atcaatattg gctattggcc attgcatacg ttgtatctat atcataatat gtacatttat
     4141 attggctcat gtccaatatg accgccatgt tgacattgat tattgactag ttattaatag
     4201 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
     4261 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
     4321 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
     4381 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tccgccccct
     4441 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttacgg
     4501 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg
     4561 ttttggcagt acaccaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
     4621 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
     4681 tgtcgtaata accccgcccc gttgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
     4741 tatataagca gagctcgttt agtgaaccgt cagatctcta gaagctgggt accagctgct
     4801 agcgccacca tgcccatggg gagtctccaa ccgctggcaa cgctgtatct tctcggtatg
     4861 ttggtggcgt cagtcttggc ggaacaactg tgggttacgg tgtattacgg tgttccggtg
     4921 tggaaagagg ctaaagcaac tctcttctgc gccagcgatg cgaaagccta cgaaaaagaa
     4981 gtacataacg tgtgggccac ccatgcttgc gtacccacag atccgaatcc ccaagagata
     5041 cccttgggta acgtgacgga aaatttcaat atgtggaaga acgacatggc agaccaaatg
     5101 cacgaagaca taattagttt gtgggaccaa agcttgaaac catgcgtcaa actgacccca
     5161 ttgtgcgtga ctctgaattg cagcgacgct acaagtaata cgacaaagaa tgcgactaac
     5221 acaaacacga cttcaacaga caaccgaaat gcgacaagta acgataccga aatgaaaggg
     5281 gaaattaaag attgtacctt taatatcaca actgaagtcc gagacagaaa gactaagcaa
     5341 cgcgcactgt tctataaact tgatgtggtc cctctggagg aggagaaaaa ctccagctct
     5401 aaaaacagct cttacaaaga gtaccgattg attagctgca acacgagcac aattacccaa
     5461 gcctgtccaa aaatgtcttt tgacccaatt ccgatccact actgcgctcc agcaggctat
     5521 gcgatactta aatgtaacaa taaaacattc aacggtaccg gaccatgtca taacgtcagt
     5581 acggttcagt gcacgcacgg gataaaacca actatgtcta cacaactgct tcttaacggt
     5641 tctcttgccg aggaggaaat catcatacgg tctgagaacc tgactgacaa cactaaaaca
     5701 attatagtgc atttgaacga gtcagtcgag attgaatgcg ttaggcccaa taataacacg
     5761 aggaaaagcg tcagaatcgg tcccgggcaa actttcttcg ctacagggga aatcataggt
     5821 gacattcgcc aggcgcattg cgatctttca aagtctaatt ggaccacgac gcttaaaagg
     5881 attgagaaga aattgaagga acacttcaat aacgctacca tcaagtttga atcttccgct
     5941 ggcggcgacc ttgagatcac tacccattct tttaattgtc gcggcgagtt cttctattgt
     6001 aatacgtctg gcctcttcaa ctcttctctg ctgaacgata cagacggaac gagcaactct
     6061 acttctaatg caaccatcac gctcccgtgc cgcataaaac aaatcatcaa tatgtggcag
     6121 gaagtggggc gggcaatgta cgcctcaccg attgccggga tcataacatg caaaagtaac
     6181 atcaccgggc tcttgctcac acgagacggt gggaataaaa gcgcagggat agagaccttt
     6241 cgcccagggg gcgggaacat gaaagataat tggcgatccg agctgtataa atacaaggtg
     6301 gttgaaatca aaccccttgg gattgcacct actagcgcga aacggcgggt cgtcgaaaga
     6361 gagaaaagag ccgcggggat cggcgcggta atattgggct ttctcggcgc agcgggttca
     6421 actatgggcg ctgctagtgt gatgcttact gtccaggcac gacagttgtt gagtggtatt
     6481 gttcagcaac aatctaacct gcttcgcgct atcgaggcac aacagcatat gttgcaactt
     6541 acagtatggg gaattaaaca actgcaaact cgggtactgg ctatcgagcg ctaccttaag
     6601 gatcagcaat tgcttggttt gtgggggtgc agtggtaaac tgatctgtac cacagcggta
     6661 ccctggaaca ccagttggtc taacaaaagc aaagacgaaa tatgggacaa catgacatgg
     6721 atgcagtggg atcgggaaat agataactac acccaggtca tctaccaact tctcgaagta
     6781 tctcagaatc agcaggagaa gaacgagaat gatctccttg ctttggataa gtggaacagc
     6841 ctgtggagtt ggtttgatat aacgaagtgg ctctggtaca ttaagatatt tatcatgatc
     6901 gttgggggtt tgattggttt gaggattatt tttgctgtat tgtcagtggt caatcgggta
     6961 cggcagggtt attccccgct ctccttccaa acgctgattc ccaatccacg gggacccgac
     7021 agaccaggag gtattgagga agagggtggc gagcaagata agaataggtc aacgagattg
     7081 gttagcggct ttttggctct tgtatgggat gacctgcgct ccttgtgtct tttttcttac
     7141 catcgactcc gagacttcat tcttatcgct gcgcgcgctg tcgaactttt ggggaggagc
     7201 agcctggaag gccttcagtg gggctgggag acacttaaat atcttcgcaa tctcgtccag
     7261 tattgggggt tggaacttaa acagtcagcg atcaacttgc tcgacacgat cgctatccaa
     7321 gtggctgagg gcacggacag aattatagaa ctgatccaga gaattttccg ggcgatctgt
     7381 aatataccta cgcgaataag gcaggggttt gaggcagccc tccagtagta actcgaggcc
     7441 ggcaaggccg gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag
     7501 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
     7561 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
     7621 tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc
     7681 tgattatgat ccggctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
     7741 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
     7801 cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaggtc gactctagag
     7861 gatcgatccc cgccccggac gaactaaacc tgactacgac atctctgccc cttcttcgcg
     7921 gggcagtgca tgtaatccct tcagttggtt ggtacaactt gccaactggg ccctgttcca
     7981 catgtgacac ggggggggac caaacacaaa ggggttctct gactgtagtt gacatcctta
     8041 taaatggatg tgcacatttg ccaacactga gtggctttca tcctggagca gactttgcag
     8101 tctgtggact gcaacacaac attgccttta tgtgtaactc ttggctgaag ctcttacacc
     8161 aatgctgggg gacatgtacc tcccaggggc ccaggaagac tacgggaggc tacaccaacg
     8221 tcaatcagag gggcctgtgt agctaccgat aagcggaccc tcaagagggc attagcaata
     8281 gtgtttataa ggcccccttg ttaaccctaa acgggtagca tatgcttccc gggtagtagt
     8341 atatactatc cagactaacc ctaattcaat agcatatgtt acccaacggg aagcatatgc
     8401 tatcgaatta gggttagtaa aagggtccta aggaacagcg atatctccca ccccatgagc
     8461 tgtcacggtt ttatttacat ggggtcagga ttccacgagg gtagtgaacc attttagtca
     8521 caagggcagt ggctgaagat caaggagcgg gcagtgaact ctcctgaatc ttcgcctgct
     8581 tcttcattct ccttcgttta gctaatagaa taactgctga gttgtgaaca gtaaggtgta
     8641 tgtgaggtgc tcgaaaacaa ggtttcaggt gacgccccca gaataaaatt tggacggggg
     8701 gttcagtggt ggcattgtgc tatgacacca atataaccct cacaaacccc ttgggcaata
     8761 aatactagtg taggaatgaa acattctgaa tatctttaac aatagaaatc catggggtgg
     8821 ggacaagccg taaagactgg atgtccatct cacacgaatt tatggctatg ggcaacacat
     8881 aatcctagtg caatatgata ctggggttat taagatgtgt cccaggcagg gaccaagaca
     8941 ggtgaaccat gttgttacac tctatttgta acaaggggaa agagagtgga cgccgacagc
     9001 agcggactcc actggttgtc tctaacaccc ccgaaaatta aacggggctc cacgccaatg
     9061 gggcccataa acaaagacaa gtggccactc ttttttttga aattgtggag tgggggcacg
     9121 cgtcagcccc cacacgccgc cctgcggttt tggactgtaa aataagggtg taataacttg
     9181 gctgattgta accccgctaa ccactgcggt caaaccactt gcccacaaaa ccactaatgg
     9241 caccccgggg aatacctgca taagtaggtg ggcgggccaa gataggggcg cgattgctgc
     9301 gatctggagg acaaattaca cacacttgcg cctgagcgcc aagcacaggg ttgttggtcc
     9361 tcatattcac gaggtcgctg agagcacggt gggctaatgt tgccatgggt agcatatact
     9421 acccaaatat ctggatagca tatgctatcc taatctatat ctgggtagca taggctatcc
     9481 taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc
     9541 taatttatat ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc
     9601 taatctatat ctgggtagta tatgctatcc taatctgtat ccgggtagca tatgctatcc
     9661 taatagagat tagggtagta tatgctatcc taatttatat ctgggtagca tatactaccc
     9721 aaatatctgg atagcatatg ctatcctaat ctatatctgg gtagcatatg ctatcctaat
     9781 ctatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat
     9841 ctatatctgg gtagtatatg ctatcctaat ttatatctgg gtagcatagg ctatcctaat
     9901 ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat
     9961 ctgtatccgg gtagcatatg ctatcctcat gcatatacag tcagcatatg atacccagta
    10021 gtagagtggg agtgctatcc tttgcatatg ccgccacctc ccaagggggc gtgaattttc
    10081 gctgcttgtc cttttcctgc tggttgctcc cattcttagg tgaatttaag gaggccaggc
    10141 taaagccgtc gcatgtctga ttgctcacca ggtaaatgtc gctaatgttt tccaacgcga
    10201 gaaggtgttg agcgcggagc tgagtgacgt gacaacatgg gtatgcccaa ttgccccatg
    10261 ttgggaggac gaaaatggtg acaagacaga tggccagaaa tacaccaaca gcacgcatga
    10321 tgtctactgg ggatttattc tttagtgcgg gggaatacac ggcttttaat acgattgagg
    10381 gcgtctccta acaagttaca tcactcctgc ccttcctcac cctcatctcc atcacctcct
    10441 tcatctccgt catctccgtc atcaccctcc gcggcagccc cttccaccat aggtggaaac
    10501 cagggaggca aatctactcc atcgtcaaag ctgcacacag tcaccctgat attgcaggta
    10561 ggagcgggct ttgtcataac aaggtcctta atcgcatcct tcaaaacctc agcaaatata
    10621 tgagtttgta aaaagaccat gaaataacag acaatggact cccttagcgg gccaggttgt
    10681 gggccgggtc caggggccat tccaaagggg agacgactca atggtgtaag acgacattgt
    10741 ggaatagcaa gggcagttcc tcgccttagg ttgtaaaggg aggtcttact acctccatat
    10801 acgaacacac cggcgaccca agttccttcg tcggtagtcc tttctacgtg actcctagcc
    10861 aggagagctc ttaaaccttc tgcaatgttc tcaaatttcg ggttggaacc tccttgacca
    10921 cgatgctttc caaaccaccc tccttttttg cgcctgcctc catcaccctg accccggggt
    10981 ccagtgcttg ggccttctcc tgggtcatct gcggggccct gctctatcgc tcccgggggc
    11041 acgtcaggct caccatctgg gccaccttct tggtggtatt caaaataatc ggcttcccct
    11101 acagggtgga aaaatggcct tctacctgga gggggcctgc gcggtggaga cccggatgat
    11161 gatgactgac tactgggact cctgggcctc ttttctccac gtccacgacc tctccccctg
    11221 gctctttcac gacttccccc cctggctctt tcacgtcctc taccccggcg gcctccacta
    11281 cctcctcgac cccggcctcc actacctcct cgaccccggc ct
//
