LOCUS       sequence-240370-        8484 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Produces equimolar expression of YPET and mTq2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8484)
  AUTHORS   Botman D, de Groot DH, Schmidt P, Goedhart J, Teusink B
  TITLE     In vivo characterisation of fluorescent proteins in budding yeast.
  JOURNAL   Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.1038/s41598-019-38913-z.
  PUBMED    30783202
REFERENCE   2  (bases 1 to 8484)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2019-02-19"; volume: "9(1):2234. doi"; pages: " 
            10.1038/s41598-019-38913-z"
COMMENT     Sequence Label: pDRF1-GW YPET-T2A-mTurquoise2
FEATURES             Location/Qualifiers
     source          1..8484
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             579..632
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      648..657
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(654..656,657..659,660..1373)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     protein_bind    complement(1383..1407)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(1469..1488)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1470..1488)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      1636..1823
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(1691..1715)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(1835..1852)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1835..1851)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1844..1866)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2287..3633
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(3744..4547)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(4548..4763)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(4788..4806)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4874..4978
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4979..5047,5048..5839)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5197..5216)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6010..6598
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6499..6518
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6752..6769
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6886..6907
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6922..6927,6928..6945,6946..6952)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6960..6976
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6965..6987
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6984..7000
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6984..7000
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(7030..7485)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7167..7188)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7379..7398
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    8293..8317
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      8334..8343
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(8340..8342,8343..8345,8346..8484,1..572)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLLCTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLL
                     EFLTAAGITEGMNELYK"
ORIGIN
        1 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg ggctacggcc
       61 tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
      121 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
      181 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
      241 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
      301 acaacgtcta tatcaccgcc gacaagcaga agaacggcat caaggccaac ttcaagatcc
      361 gccacaacat cgaggacggc ggcgtgcagc tcgccgacca ctaccagcag aacaccccca
      421 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagctaccag tccgccctgt
      481 tcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcctg accgccgccg
      541 ggatcactga gggcatgaac gagctgtaca agtccggaga gggcagagga agtcttctaa
      601 catgcggtga cgtggaggag aatcccggcc ctggggatcc accggtcgcc accatggtga
      661 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
      721 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
      781 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
      841 ccaccctgtc ctggggcgtg cagtgcttcg cccgctaccc cgaccacatg aagcagcacg
      901 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
      961 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     1021 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     1081 agtacaacta ctttagcgac aacgtctata tcaccgccga caagcagaag aacggcatca
     1141 aggccaactt caagatccgc cacaacatcg aggacggcgg cgtgcagctc gccgaccact
     1201 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     1261 gcacccagtc caagctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     1321 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag taaagcggcc
     1381 gcacccagct ttcttgtaca aagtggtgta gttaattcat gatggccgct gcaggtcgac
     1441 ctcgaggggg ggcccggtac ccaattcgcc ctatagtgag tcgtattacg cgcggatcca
     1501 gctttggact tcttcgccag aggtttggtc aagtctccaa tcaaggttgt cggcttgtct
     1561 accttgccag aaatttacga aaagatggaa aagggtcaaa tcgttggtag atacgttgtt
     1621 gacacttcta aataagcgaa tttcttatga tttatgattt ttattattaa ataagttata
     1681 aaaaaaataa gtgtatacaa attttaaagt gactcttagg ttttaaaacg aaaattctta
     1741 ttcttgagta actctttcct gtaggtcagg ttgctttctc aggtatagca tgaggtcgct
     1801 cttattgacc acacctctac cggcatgcca attcactggc cgtcgtttta caacgtcgtg
     1861 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     1921 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     1981 atggcgaatg gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     2041 gcataatcgg atcgtacttg ttacccatca ttgaattttg aacatccgaa cctgggagtt
     2101 ttccctgaaa cagatagtat atttgaacct gtataataat atatagtcta gcgctttacg
     2161 gaagacaatg tatgtatttc ggttcctgga gaaactattg catctattgc ataggtaatc
     2221 ttgcacgtcg catccccggt tcattttctg cgtttccatc ttgcacttca atagcatatc
     2281 tttgttaacg aagcatctgt gcttcatttt gtagaacaaa aatgcaacgc gagagcgcta
     2341 atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgaaagcg
     2401 ctattttacc aacgaagaat ctgtgcttca tttttgtaaa acaaaaatgc aacgcgagag
     2461 cgctaatttt tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga
     2521 gagcgctatt ttaccaacaa agaatctata cttctttttt gttctacaaa aatgcatccc
     2581 gagagcgcta tttttctaac aaagcatctt agattacttt ttttctcctt tgtgcgctct
     2641 ataatgcagt ctcttgataa ctttttgcac tgtaggtccg ttaaggttag aagaaggcta
     2701 ctttggtgtc tattttctct tccataaaaa aagcctgact ccacttcccg cgtttactga
     2761 ttactagcga agctgcgggt gcattttttc aagataaagg catccccgat tatattctat
     2821 accgatgtgg attgcgcata ctttgtgaac agaaagtgat agcgttgatg attcttcatt
     2881 ggtcagaaaa ttatgaacgg tttcttctat tttgtctcta tatactacgt ataggaaatg
     2941 tttacatttt cgtattgttt tcgattcact ctatgaatag ttcttactac aatttttttg
     3001 tctaaagagt aatactagag ataaacataa aaaatgtaga ggtcgagttt agatgcaagt
     3061 tcaaggagcg aaaggtggat gggtaggtta tatagggata tagcacagag atatatagca
     3121 aagagatact tttgagcaat gtttgtggaa gcggtattcg caatatttta gtagctcgtt
     3181 acagtccggt gcgtttttgg ttttttgaaa gtgcgtcttc agagcgcttt tggttttcaa
     3241 aagcgctctg aagttcctat actttctagc tagagaatag gaacttcgga ataggaactt
     3301 caaagcgttt ccgaaaacga gcgcttccga aaatgcaacg cgagctgcgc acatacagct
     3361 cactgttcac gtcgcaccta tatctgcgtg ttgcctgtat atatatatac atgagaagaa
     3421 cggcatagtg cgtgtttatg cttaaatgcg tacttatatg cgtctattta tgtaggatga
     3481 aaggtagtct agtacctcct gtgatattat cccattccat gcggggtatc gtatgcttcc
     3541 ttcagcacta ccctttagct gttctatatg ctgccactcc tcaattggat tagtctcatc
     3601 cttcaatgct atcatttcct ttgatattgg atcgatccga tgataagctg tcaaacatga
     3661 gaattgggta ataactgata taattaaatt gaagctctaa tttgtgagtt tagtatacat
     3721 gcatttactt ataatacagt tttttagttt tgctggccgc atcttctcaa atatgcttcc
     3781 cagcctgctt ttctgtaacg ttcaccctct accttagcat cccttccctt tgcaaatagt
     3841 cctcttccaa caataataat gtcagatcct gtagagacca catcatccac ggttctatac
     3901 tgttgaccca atgcgtctcc cttgtcatct aaacccacac cgggtgtcat aatcaaccaa
     3961 tcgtaacctt catctcttcc acccatgtct ctttgagcaa taaagccgat aacaaaatct
     4021 ttgtcgctct tcgcaatgtc aacagtaccc ttagtatatt ctccagtaga tagggagccc
     4081 ttgcatgaca attctgctaa catcaaaagg cctctaggtt cctttgttac ttcttctgcc
     4141 gcctgcttca aaccgctaac aatacctggg cccaccacac cgtgtgcatt cgtaatgtct
     4201 gcccattctg ctattctgta tacacccgca gagtactgca atttgactgt attaccaatg
     4261 tcagcaaatt ttctgtcttc gaagagtaaa aaattgtact tggcggataa tgcctttagc
     4321 ggcttaactg tgccctccat ggaaaaatca gtcaagatat ccacatgtgt ttttagtaaa
     4381 caaattttgg gacctaatgc ttcaactaac tccagtaatt ccttggtggt acgaacatcc
     4441 aatgaagcac acaagtttgt ttgcttttcg tgcatgatat taaatagctt ggcagcaaca
     4501 ggactaggat gagtagcagc acgttcctta tatgtagctt tcgacatgat ttatcttcgt
     4561 ttcctgcatg tttttgttct gtgcagttgg gttaagaata ctgggcaatt tcatgtttct
     4621 tcaacactac atatgcgtat atataccaat ctaagtctgt gctccttcct tcgttcttcc
     4681 ttctgttcgg agattaccga atcaaaaaaa tttcaaggaa accgaaatca aaaaaaagaa
     4741 taaaaaaaaa atgatgaatt gaaaagctaa ttcttgaaga cgaaagggcc tcgtgatacg
     4801 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     4861 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     4921 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     4981 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     5041 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     5101 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     5161 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     5221 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     5281 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     5341 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     5401 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     5461 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     5521 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     5581 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     5641 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     5701 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     5761 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     5821 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     5881 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     5941 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     6001 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     6061 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     6121 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     6181 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     6241 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     6301 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     6361 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     6421 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     6481 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     6541 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     6601 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     6661 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     6721 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     6781 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     6841 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     6901 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     6961 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttac
     7021 cgcatcagga aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa
     7081 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
     7141 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
     7201 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac
     7261 catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta
     7321 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag
     7381 ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg
     7441 taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtccatt cgccaagctt
     7501 cctgaaacgg agaaacataa acaggcattg ctgggatcac ccatacatca ctctgttttg
     7561 cctgaccttt tccggtaatt tgaaaacaaa cccggtctcg aagcggagat ccggcgataa
     7621 ttaccgcaga aataaaccca tacacgagac gtagaaccag ccgcacatgg ccggagaaac
     7681 tcctgcgaga atttcgtaaa ctcgcgcgca ttgcatctgt atttcctaat gcggcacttc
     7741 caggcctcga tcgagaccgt ttatccattg cttttttgtt gtctttttcc ctcgttcaca
     7801 gaaagtctga agaagctata gtagaactat gagctttttt tgtttctgtt ttcctttttt
     7861 ttttttttac ctctgtggaa attgttactc tcacactctt tagttcgttt gtttgttttg
     7921 tttattccaa ttatgaccgg tgacgaaacg tggtcgatgg tgggtaccgc ttatgctccc
     7981 ctccattagt ttcgattata taaaaaggcc aaatattgta ttattttcaa atgtcctatc
     8041 attatcgtct aacatctaat ttctcttaaa ttttttctct ttctttccta taacaccaat
     8101 agtgaaaatc tttttttctt ctatatctac aaaaactttt tttttctatc aacctcgttg
     8161 ataaattttt tctttaacaa tcgttaataa ttaattaatt ggaaaataac cattttttct
     8221 ctcttttata cacacattca aaagaaagaa aaaaaatata ccccagcctc gactagtgaa
     8281 ttcgcggcca tcacaagttt gtacaaaaaa gcaggctgct agcgctaccg gtcgccacca
     8341 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     8401 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
     8461 gcaagctgac cctgaagctt ctat
//
