LOCUS       sequence-240377-       10680 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the Q842.d12 HIV-1 
            isolate; C-terminal truncation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10680)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 10680)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-Q842gp160-754*
FEATURES             Location/Qualifiers
     source          1..10680
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(711..729)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        797..901
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(902..970,971..1762)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1120..1139)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1933..2521
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2422..2441
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2952..3097
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3140..4177
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4175..4194)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4219..4267
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4921..5300
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5301..5504
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5455..5475
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5501..5525
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     polyA_signal    7795..7929
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7819..7838)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7873..7892
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(8072..8094)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8654..10443
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 tcctgctcct gttccaccgt gggtcccttt gcagccaatg caacttggac gtttttgggg
       61 tctccggaca ccatctctat gtcttggccc tgatcctgag ccgcccgggg ctcctggtct
      121 tccgcctcct cgtcctcgtc ctcttccccg tcctcgtcca tggttatcac cccctcttct
      181 ttgaggtcca ctgccgccgg agccttctgg tccagatgtg tctcccttct ctcctaggcc
      241 atttccaggt cctgtacctg gcccctcgtc agacatgatt cacactaaaa gagatcaata
      301 gacatcttta ttagacgacg ctcagtgaat acagggagtg cagactcctg ccccctccaa
      361 cagccccccc accctcatcc ccttcatggt cgctgtcaga cagatccagg tctgaaaatt
      421 ccccatcctc cgaaccatcc tcgtcctcat caccaattac tcgcagcccg gaaaactccc
      481 gctgaacatc ctcaagattt gcgtcctgag cctcaagcca ggcctcaaat tcctcgtccc
      541 cctttttgct ggacggtagg gatggggatt ctcgggaccc ctcctcttcc tcttcaaggt
      601 caccagacag agatgctact ggggcaacgg aagaaaagct gggtgcggcc tgtgaggatc
      661 agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat
      721 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
      781 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
      841 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
      901 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
      961 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     1021 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     1081 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     1141 ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     1201 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     1261 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     1321 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     1381 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     1441 gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     1501 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     1561 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     1621 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     1681 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     1741 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     1801 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     1861 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     1921 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     1981 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     2041 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     2101 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     2161 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     2221 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     2281 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     2341 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     2401 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     2461 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     2521 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt gaagctgtcc
     2581 ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc
     2641 gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag
     2701 caagacgtag cccagcgcgt cggccccgag atgcgccgcg tgcggctgct ggagatggcg
     2761 gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat
     2821 tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccc tgcttcatcc
     2881 ccgtggcccg ttgctcgcgt ttgctggcgg tgtccccgga agaaatatat ttgcatgtct
     2941 ttagttctat gatgacacaa accccgccca gcgtcttgtc attggcgaat tcgaacacgc
     3001 agatgcagtc ggggcggcgc ggtccgaggt ccacttcgca tattaaggtg acgcgtgtgg
     3061 cctcgaacac cgagcgaccc tgcagcgacc cgcttaacag cgtcaacagc gtgccgcaga
     3121 tcccgggggg caatgagata tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt
     3181 tctgatcgaa aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc
     3241 tcgtgctttc agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc
     3301 cgatggtttc tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat
     3361 tccggaagtg cttgacattg gggaattcag cgagagcctg acctattgca tctcccgccg
     3421 tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctgcagcc
     3481 ggtcgcggag gccatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg
     3541 cccattcgga ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat
     3601 tgctgatccc catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt
     3661 cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct
     3721 cgtgcacgcg gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt
     3781 cattgactgg agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt
     3841 ctggaggccg tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc
     3901 ggagcttgca ggatcgccgc ggctccgggc gtatatgctc cgcattggtc ttgaccaact
     3961 ctatcagagc ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga
     4021 cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc
     4081 ggccgtctgg accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag
     4141 cactcgtccg gatcgggaga tgggggaggc taactgaaac acggaaggag acaataccgg
     4201 aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggg tgttgggtcg
     4261 tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga
     4321 ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc cccaagttcg
     4381 ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat agccactggc
     4441 cccgtgggtt agggacgggg tcccccatgg ggaatggttt atggttcgtg ggggttatta
     4501 ttttgggcgt tgcgtggggt caggtccacg actggactga gcagacagac ccatggtttt
     4561 tggatggcct gggcatggac cgcatgtact ggcgcgacac gaacaccggg cgtctgtggc
     4621 tgccaaacac ccccgacccc caaaaaccac cgcgcggatt tctggcgtgc caagctagtc
     4681 gaccaattct catgtttgac agcttatcat cgcagatccg ggcaacgttg ttgccattgc
     4741 tgcaggcgca gaactggtag gtatggaaga tctatacatt gaatcaatat tggcaattag
     4801 ccatattagt cattggttat atagcataaa tcaatattgg ctattggcca ttgcatacgt
     4861 tgtatctata tcataatatg tacatttata ttggctcatg tccaatatga ccgccatgtt
     4921 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
     4981 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
     5041 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
     5101 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
     5161 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
     5221 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
     5281 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
     5341 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
     5401 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
     5461 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
     5521 agatctctag aagctgggta ccagctgcta gcgccaccat gccaatggga tcactgcagc
     5581 ccttggcaac gctgtacttg cttggtatgc ttgtggcatc tgttcttgct gtcgataatt
     5641 tgtgggtcac agtgtactac ggagtacctg tatggaaaga ggcggaaact actctttttt
     5701 gcgcgtcaga cgcaaaggca tatgagactg aaaagcataa tgtatgggct acccatgcgt
     5761 gcgtgcccac agaccccaac ccccaagaaa tccatctgga gaacgttact gaagaattta
     5821 atatgtggaa gaataatatg gtggaacaga tgcatacgga cattatcagt ctgtgggatc
     5881 agtctctgaa accatgtgtt aaactcaccc ctctctgcgt aaccttggac tgtaataacg
     5941 tgacgaataa tggaactagt gacatgaggg aagagataaa gaattgctcc ttcaatatga
     6001 caacagaatt gcgagacaag cgacaaaaag tatattctct gttctacaaa ttggacatag
     6061 tgcagataaa tgaggatcaa ggtaacagct ccaataacaa gtatcgcctg ataacttgca
     6121 acacgtctgc gattacccaa gcatgtccta aggttacatt tgaacccatt ccgattcatt
     6181 attgcgcacc tgcaggcttt gctatcctga agtgcaaaga tgaagagttt aacgggatcg
     6241 gcccgtgcaa aaacgtatcc actgtccaat gcacccacgg tattaagcct gttgtgtcca
     6301 cacagctcct tctcaatggg tctctggcag agaaggaggt caaaataaga tgtgaaaata
     6361 taacgaacaa cgcaaaaact atcattgtcc aactggtgaa cccagttaaa atcaattgca
     6421 cccgacctaa taataatacg agaaagagta tccacatcgg cccagggcag gccttctacg
     6481 ctacaggcga tataatcgga gacataagac aggcgcactg caatgtaaat cgtacggaat
     6541 ggaataatac tctccaccag gtagttgagc agttgcgaaa gcattttaat aaaacgatta
     6601 actttgctaa ctcaaccgga ggggacttgg aaataacaac acactcattc aattgcgggg
     6661 gcgaattttt ttattgtaac acgactaacc tgtttaatag cacttggaac catacggcta
     6721 gtatgaactc aaccgaatcc aatgacacaa tcatcctgcc ctgtcgaata aaacaaatca
     6781 tcaatatgtg gcaaagagtg ggccaagcaa tgtatgcacc cccaataaga ggtgtcataa
     6841 ggtgtgaatc caatataact gggttgattc ttactaggga tggaggaaac acgaacagca
     6901 cccgagaaac tttccgccca ggcgggggtg atatgaggga taattggcga tcagaacttt
     6961 acaagtataa ggtagttaag atagaacccc tcggcgttgc tcccacaaaa gccaagagaa
     7021 gggttgtaga acgcgaaaag cgggccgtag ggattggcgc tgtattcatc ggatttctcg
     7081 gagcagctgg atctactatg ggtgcagctt caataacatt gacagttcaa gcccggcaac
     7141 tcctttcagg tattgtacag cagcaaagta acctcctgcg agcgattgag gctcagcagc
     7201 atctcctgaa gcttacggtg tggggtatca aacagcttca agcgcgggta ctcgcggttg
     7261 agcgatacct taaggaccaa caattgcttg gaatctgggg ctgcagcggt aaacttatat
     7321 gtacaacatc tgtgccgtgg aacagttcct ggagtaacaa atcacaaaat gagatttggg
     7381 acaatatgac ctggttgcag tgggataaag aaatatcaaa ctatacccag atcatttacg
     7441 atctcttgga ggagtctcaa aaccagcagg agaaaaacga acaggacctc ctcgcattgg
     7501 ataaatgggc caatttgtgg aactggtttg acatatctaa ctggctttgg tatataaaga
     7561 tattcatcat gattgtaggg ggtcttattg gtctgaggat agttttcgca gtcttgtcag
     7621 tcattaaccg agtgcggcag ggttattctc ccttgtcatt tcaaactcac actcctaatc
     7681 cgaggggact ggataggcct gagcgaatag aggaagaggg cggtgagcaa gacaagaacc
     7741 ggtcaatcag gctcgtgtct ggtttcctgt gataactcga ggccggcaag gccggatcca
     7801 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
     7861 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
     7921 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg
     7981 gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta tgatccggct
     8041 gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     8101 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     8161 gtgttggcgg gtgtcggggc gcagccatga ggtcgactct agaggatcga tccccgcccc
     8221 ggacgaacta aacctgacta cgacatctct gccccttctt cgcggggcag tgcatgtaat
     8281 cccttcagtt ggttggtaca acttgccaac tgggccctgt tccacatgtg acacgggggg
     8341 ggaccaaaca caaaggggtt ctctgactgt agttgacatc cttataaatg gatgtgcaca
     8401 tttgccaaca ctgagtggct ttcatcctgg agcagacttt gcagtctgtg gactgcaaca
     8461 caacattgcc tttatgtgta actcttggct gaagctctta caccaatgct gggggacatg
     8521 tacctcccag gggcccagga agactacggg aggctacacc aacgtcaatc agaggggcct
     8581 gtgtagctac cgataagcgg accctcaaga gggcattagc aatagtgttt ataaggcccc
     8641 cttgttaacc ctaaacgggt agcatatgct tcccgggtag tagtatatac tatccagact
     8701 aaccctaatt caatagcata tgttacccaa cgggaagcat atgctatcga attagggtta
     8761 gtaaaagggt cctaaggaac agcgatatct cccaccccat gagctgtcac ggttttattt
     8821 acatggggtc aggattccac gagggtagtg aaccatttta gtcacaaggg cagtggctga
     8881 agatcaagga gcgggcagtg aactctcctg aatcttcgcc tgcttcttca ttctccttcg
     8941 tttagctaat agaataactg ctgagttgtg aacagtaagg tgtatgtgag gtgctcgaaa
     9001 acaaggtttc aggtgacgcc cccagaataa aatttggacg gggggttcag tggtggcatt
     9061 gtgctatgac accaatataa ccctcacaaa ccccttgggc aataaatact agtgtaggaa
     9121 tgaaacattc tgaatatctt taacaataga aatccatggg gtggggacaa gccgtaaaga
     9181 ctggatgtcc atctcacacg aatttatggc tatgggcaac acataatcct agtgcaatat
     9241 gatactgggg ttattaagat gtgtcccagg cagggaccaa gacaggtgaa ccatgttgtt
     9301 acactctatt tgtaacaagg ggaaagagag tggacgccga cagcagcgga ctccactggt
     9361 tgtctctaac acccccgaaa attaaacggg gctccacgcc aatggggccc ataaacaaag
     9421 acaagtggcc actctttttt ttgaaattgt ggagtggggg cacgcgtcag cccccacacg
     9481 ccgccctgcg gttttggact gtaaaataag ggtgtaataa cttggctgat tgtaaccccg
     9541 ctaaccactg cggtcaaacc acttgcccac aaaaccacta atggcacccc ggggaatacc
     9601 tgcataagta ggtgggcggg ccaagatagg ggcgcgattg ctgcgatctg gaggacaaat
     9661 tacacacact tgcgcctgag cgccaagcac agggttgttg gtcctcatat tcacgaggtc
     9721 gctgagagca cggtgggcta atgttgccat gggtagcata tactacccaa atatctggat
     9781 agcatatgct atcctaatct atatctgggt agcataggct atcctaatct atatctgggt
     9841 agcatatgct atcctaatct atatctgggt agtatatgct atcctaattt atatctgggt
     9901 agcataggct atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt
     9961 agtatatgct atcctaatct gtatccgggt agcatatgct atcctaatag agattagggt
    10021 agtatatgct atcctaattt atatctgggt agcatatact acccaaatat ctggatagca
    10081 tatgctatcc taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagca
    10141 taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta
    10201 tatgctatcc taatttatat ctgggtagca taggctatcc taatctatat ctgggtagca
    10261 tatgctatcc taatctatat ctgggtagta tatgctatcc taatctgtat ccgggtagca
    10321 tatgctatcc tcatgcatat acagtcagca tatgataccc agtagtagag tgggagtgct
    10381 atcctttgca tatgccgcca cctcccaagg gggcgtgaat tttcgctgct tgtccttttc
    10441 ctgctggttg ctcccattct taggtgaatt taaggaggcc aggctaaagc cgtcgcatgt
    10501 ctgattgctc accaggtaaa tgtcgctaat gttttccaac gcgagaaggt gttgagcgcg
    10561 gagctgagtg acgtgacaac atgggtatgc ccaattgccc catgttggga ggacgaaaat
    10621 ggtgacaaga cagatggcca gaaatacacc aacagcacgc atgatgtcta ctggggattt
//
