LOCUS       sequence-240380-       11609 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the Q842.d12 HIV-1 
            isolate; QES mutant with enhanced binding to PG16.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11609)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 11609)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-Q842-QES.i04.c05
FEATURES             Location/Qualifiers
     source          1..11609
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(677..695)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        763..867
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(868..936,937..1728)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1086..1105)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1899..2487
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2388..2407
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2918..3063
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3106..4143
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4141..4160)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4185..4233
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4887..5266
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5267..5470
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5421..5441
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5467..5491
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     polyA_signal    8091..8225
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8115..8134)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8169..8188
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(8368..8390)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8950..10739
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 ccaatgcaac ttggacgttt ttggggtctc cggacaccat ctctatgtct tggccctgat
       61 cctgagccgc ccggggctcc tggtcttccg cctcctcgtc ctcgtcctct tccccgtcct
      121 cgtccatggt tatcaccccc tcttctttga ggtccactgc cgccggagcc ttctggtcca
      181 gatgtgtctc ccttctctcc taggccattt ccaggtcctg tacctggccc ctcgtcagac
      241 atgattcaca ctaaaagaga tcaatagaca tctttattag acgacgctca gtgaatacag
      301 ggagtgcaga ctcctgcccc ctccaacagc ccccccaccc tcatcccctt catggtcgct
      361 gtcagacaga tccaggtctg aaaattcccc atcctccgaa ccatcctcgt cctcatcacc
      421 aattactcgc agcccggaaa actcccgctg aacatcctca agatttgcgt cctgagcctc
      481 aagccaggcc tcaaattcct cgtccccctt tttgctggac ggtagggatg gggattctcg
      541 ggacccctcc tcttcctctt caaggtcacc agacagagat gctactgggg caacggaaga
      601 aaagctgggt gcggcctgtg aggatcagct tatcgatgat aagctgtcaa acatgagaat
      661 tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
      721 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
      781 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
      841 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
      901 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
      961 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     1021 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     1081 gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc
     1141 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     1201 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     1261 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     1321 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     1381 ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta
     1441 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     1501 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     1561 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     1621 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     1681 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     1741 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     1801 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     1861 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     1921 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     1981 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     2041 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     2101 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     2161 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     2221 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     2281 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     2341 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     2401 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     2461 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     2521 gccttttgct ggccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg
     2581 gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc
     2641 atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cccgagatgc
     2701 gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt
     2761 gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt
     2821 tagcgaggtg ccgccctgct tcatccccgt ggcccgttgc tcgcgtttgc tggcggtgtc
     2881 cccggaagaa atatatttgc atgtctttag ttctatgatg acacaaaccc cgcccagcgt
     2941 cttgtcattg gcgaattcga acacgcagat gcagtcgggg cggcgcggtc cgaggtccac
     3001 ttcgcatatt aaggtgacgc gtgtggcctc gaacaccgag cgaccctgca gcgacccgct
     3061 taacagcgtc aacagcgtgc cgcagatccc ggggggcaat gagatatgaa aaagcctgaa
     3121 ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg
     3181 atgcagctct cggagggcga agaatctcgt gctttcagct tcgatgtagg agggcgtgga
     3241 tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg
     3301 cactttgcat cggccgcgct cccgattccg gaagtgcttg acattgggga attcagcgag
     3361 agcctgacct attgcatctc ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa
     3421 accgaactgc ccgctgttct gcagccggtc gcggaggcca tggatgcgat cgctgcggcc
     3481 gatcttagcc agacgagcgg gttcggccca ttcggaccgc aaggaatcgg tcaatacact
     3541 acatggcgtg atttcatatg cgcgattgct gatccccatg tgtatcactg gcaaactgtg
     3601 atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat gctttgggcc
     3661 gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa caatgtcctg
     3721 acggacaatg gccgcataac agcggtcatt gactggagcg aggcgatgtt cggggattcc
     3781 caatacgagg tcgccaacat cttcttctgg aggccgtggt tggcttgtat ggagcagcag
     3841 acgcgctact tcgagcggag gcatccggag cttgcaggat cgccgcggct ccgggcgtat
     3901 atgctccgca ttggtcttga ccaactctat cagagcttgg ttgacggcaa tttcgatgat
     3961 gcagcttggg cgcagggtcg atgcgacgca atcgtccgat ccggagccgg gactgtcggg
     4021 cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt agaagtactc
     4081 gccgatagtg gaaaccgacg ccccagcact cgtccggatc gggagatggg ggaggctaac
     4141 tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca
     4201 gaataaaacg cacgggtgtt gggtcgtttg ttcataaacg cggggttcgg tcccagggct
     4261 ggcactctgt cgatacccca ccgagacccc attggggcca atacgcccgc gtttcttcct
     4321 tttccccacc ccacccccca agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg
     4381 cggcaggccc tgccatagcc actggccccg tgggttaggg acggggtccc ccatggggaa
     4441 tggtttatgg ttcgtggggg ttattatttt gggcgttgcg tggggtcagg tccacgactg
     4501 gactgagcag acagacccat ggtttttgga tggcctgggc atggaccgca tgtactggcg
     4561 cgacacgaac accgggcgtc tgtggctgcc aaacaccccc gacccccaaa aaccaccgcg
     4621 cggatttctg gcgtgccaag ctagtcgacc aattctcatg tttgacagct tatcatcgca
     4681 gatccgggca acgttgttgc cattgctgca ggcgcagaac tggtaggtat ggaagatcta
     4741 tacattgaat caatattggc aattagccat attagtcatt ggttatatag cataaatcaa
     4801 tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg
     4861 ctcatgtcca atatgaccgc catgttgaca ttgattattg actagttatt aatagtaatc
     4921 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     4981 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     5041 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     5101 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtccgc cccctattga
     5161 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tacgggactt
     5221 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     5281 gcagtacacc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     5341 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     5401 taataacccc gccccgttga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     5461 aagcagagct cgtttagtga accgtcagat ctctagaagc tgggtaccag ctgctagcgc
     5521 caccatgcca atgggatcac tgcagccctt ggcaacgctg tacttgcttg gtatgcttgt
     5581 ggcatctgtt cttgctgtcg ataatttgtg ggtcacagtg tactacggag tacctgtatg
     5641 gaaagaggcg gaaactactc ttttttgcgc gtcagacgca aaggcatatg agactgaaaa
     5701 gcataatgta tgggctaccc atgcgtgcgt gcccacagac cccaaccccc aagaaatcca
     5761 tctggagaac gttactgaag aatttaatat gtggaagaat aatatggtgg aacagatgca
     5821 tacggacatt atcagtctgt gggatcagtc tctgaaacca tgtgttaaac tcaccgatct
     5881 ctgcgtaacc ttggactgta ataacgtgac gaataatgga actagtgaca tgagggaaga
     5941 gataaagaat tgctccttca atatgacaac agaattgcga gacaagcgac aaaaagtata
     6001 ttctctgttc tacaaattgg acctggtgca gataaatgag gatcaaggta acagctccaa
     6061 taacaagtat cgcctgataa cttgcaacac gtctgcgatt acccaagcat gtcctaagat
     6121 gacatttgaa cccattccga ttcattattg cgcacctgca ggctatgcta tcctgaagtg
     6181 caaagatgaa gagtttaacg ggatcggccc gtgcaaaaac gtatccactg tccaatgcac
     6241 ccacggtatt aagcctgttg tgtccacaca gctccttctc aatgggtctc tggcagagaa
     6301 ggaggtcaaa ataagatgtg aaaatataac gaacaacgca aaacctatca ttgtccaact
     6361 ggtgaaccca gttaaaatca attgcacccg acctaataat aatacgagaa agagtatcca
     6421 catcggccca gggcaggcct tctacgctac aggcgatata atcggagaca taagacaggc
     6481 gcactgcaat gtaaatcgta cggaatggaa taatactctc caccaggtag ttgagcagtt
     6541 gcgaaagcat tttaataaaa cgattaactt tgctaactca accggagggg acttggaaat
     6601 aacaacacac tcattcaatt gcgggggcga atggttttat tgtaacacga ctaacctgtt
     6661 taatagcact tggaaccata cggctagtat gaactcaacc gaatccaatg acacaatcat
     6721 cctgccctgt cgaataaaac aaatcatcaa tatgtggcaa agagtgggcc aagcaatgta
     6781 tgcaccccca ataagaggtg tcataaggtg tgaatccaat ataactgggt tgattcttac
     6841 tagggatgga ggaaacacga acagcacccg agaaactttc cgcccaggcg ggggtgatat
     6901 gagggataat tggcgatcag aactttacaa gtataaggta gttaagatag aacccctcgg
     6961 cgttgctccc acaaaagcca agagaagggt tgtagaacgc gaaaagcggg ccgtagggat
     7021 tggcgctgta ttcatcggat ttctcggagc agctggatct actatgggtg cagcttcaat
     7081 aacattgaca gttcaagccc ggcaactcct ttcaggtatt gtacagcagc aaagtaacct
     7141 cctgcaagcg attgaggctc agcagcatct cctgaagctt acggtgtggg gtatcaaaca
     7201 gcttcaagcg cgggtactcg cggttgagcg ataccttaag gaccaacaat tgcttggaat
     7261 gtggggctgc agcggtaaac ttatatgtac aacatctgtg ccgtggaaca gttcctggag
     7321 taacaaatca caaaatgaga tttgggacaa tatgacctgg ttgcagtggg ataaagaaat
     7381 atcaaactat acccagatca tttacgatct cttggaggag tctcaaaacc agcaggagaa
     7441 aaacgaacag gacctcctcg cattggataa atgggccaat ttgtggaact ggtttgacat
     7501 atctaactgg ctttggtata taaagatatt catcatgatt gtagggggtc ttattggtct
     7561 gaggatagtt ttcgcagtct tgtcagtcat taaccgagtg cggcagggtt attctccctt
     7621 gtcatttcaa actcacactc ctaatccgag gggactggat aggcctgagc gaatagagga
     7681 agagggcggt gagcaagaca agaaccggtc aatcaggctc gtgtctggtt tcctggcact
     7741 ggcgtgggat gatctgagga gtctttgtct tttctcatac catcggttgc gcgacttcat
     7801 actcatcgtc gccaggaccg tagaactttt gggacattcc tccttgaagg gacttcggct
     7861 cggatgggag ggattgaagt acttggggaa tctgctgagt tattgggggc gggaattgcg
     7921 aatctccgcc acaaatctct tggatacgat tgctatagta atagctggat ggacggatag
     7981 agtaatcgaa atagggcagc gcctctgtcg agcatttctc aatatcccca gaagaataag
     8041 gcaaggattt gagcgcgctc tgctctagta actcgaggcc ggcaaggccg gatccagaca
     8101 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
     8161 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
     8221 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
     8281 ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat ccggctgcct
     8341 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
     8401 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
     8461 tggcgggtgt cggggcgcag ccatgaggtc gactctagag gatcgatccc cgccccggac
     8521 gaactaaacc tgactacgac atctctgccc cttcttcgcg gggcagtgca tgtaatccct
     8581 tcagttggtt ggtacaactt gccaactggg ccctgttcca catgtgacac ggggggggac
     8641 caaacacaaa ggggttctct gactgtagtt gacatcctta taaatggatg tgcacatttg
     8701 ccaacactga gtggctttca tcctggagca gactttgcag tctgtggact gcaacacaac
     8761 attgccttta tgtgtaactc ttggctgaag ctcttacacc aatgctgggg gacatgtacc
     8821 tcccaggggc ccaggaagac tacgggaggc tacaccaacg tcaatcagag gggcctgtgt
     8881 agctaccgat aagcggaccc tcaagagggc attagcaata gtgtttataa ggcccccttg
     8941 ttaaccctaa acgggtagca tatgcttccc gggtagtagt atatactatc cagactaacc
     9001 ctaattcaat agcatatgtt acccaacggg aagcatatgc tatcgaatta gggttagtaa
     9061 aagggtccta aggaacagcg atatctccca ccccatgagc tgtcacggtt ttatttacat
     9121 ggggtcagga ttccacgagg gtagtgaacc attttagtca caagggcagt ggctgaagat
     9181 caaggagcgg gcagtgaact ctcctgaatc ttcgcctgct tcttcattct ccttcgttta
     9241 gctaatagaa taactgctga gttgtgaaca gtaaggtgta tgtgaggtgc tcgaaaacaa
     9301 ggtttcaggt gacgccccca gaataaaatt tggacggggg gttcagtggt ggcattgtgc
     9361 tatgacacca atataaccct cacaaacccc ttgggcaata aatactagtg taggaatgaa
     9421 acattctgaa tatctttaac aatagaaatc catggggtgg ggacaagccg taaagactgg
     9481 atgtccatct cacacgaatt tatggctatg ggcaacacat aatcctagtg caatatgata
     9541 ctggggttat taagatgtgt cccaggcagg gaccaagaca ggtgaaccat gttgttacac
     9601 tctatttgta acaaggggaa agagagtgga cgccgacagc agcggactcc actggttgtc
     9661 tctaacaccc ccgaaaatta aacggggctc cacgccaatg gggcccataa acaaagacaa
     9721 gtggccactc ttttttttga aattgtggag tgggggcacg cgtcagcccc cacacgccgc
     9781 cctgcggttt tggactgtaa aataagggtg taataacttg gctgattgta accccgctaa
     9841 ccactgcggt caaaccactt gcccacaaaa ccactaatgg caccccgggg aatacctgca
     9901 taagtaggtg ggcgggccaa gataggggcg cgattgctgc gatctggagg acaaattaca
     9961 cacacttgcg cctgagcgcc aagcacaggg ttgttggtcc tcatattcac gaggtcgctg
    10021 agagcacggt gggctaatgt tgccatgggt agcatatact acccaaatat ctggatagca
    10081 tatgctatcc taatctatat ctgggtagca taggctatcc taatctatat ctgggtagca
    10141 tatgctatcc taatctatat ctgggtagta tatgctatcc taatttatat ctgggtagca
    10201 taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta
    10261 tatgctatcc taatctgtat ccgggtagca tatgctatcc taatagagat tagggtagta
    10321 tatgctatcc taatttatat ctgggtagca tatactaccc aaatatctgg atagcatatg
    10381 ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagcatagg
    10441 ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg
    10501 ctatcctaat ttatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg
    10561 ctatcctaat ctatatctgg gtagtatatg ctatcctaat ctgtatccgg gtagcatatg
    10621 ctatcctcat gcatatacag tcagcatatg atacccagta gtagagtggg agtgctatcc
    10681 tttgcatatg ccgccacctc ccaagggggc gtgaattttc gctgcttgtc cttttcctgc
    10741 tggttgctcc cattcttagg tgaatttaag gaggccaggc taaagccgtc gcatgtctga
    10801 ttgctcacca ggtaaatgtc gctaatgttt tccaacgcga gaaggtgttg agcgcggagc
    10861 tgagtgacgt gacaacatgg gtatgcccaa ttgccccatg ttgggaggac gaaaatggtg
    10921 acaagacaga tggccagaaa tacaccaaca gcacgcatga tgtctactgg ggatttattc
    10981 tttagtgcgg gggaatacac ggcttttaat acgattgagg gcgtctccta acaagttaca
    11041 tcactcctgc ccttcctcac cctcatctcc atcacctcct tcatctccgt catctccgtc
    11101 atcaccctcc gcggcagccc cttccaccat aggtggaaac cagggaggca aatctactcc
    11161 atcgtcaaag ctgcacacag tcaccctgat attgcaggta ggagcgggct ttgtcataac
    11221 aaggtcctta atcgcatcct tcaaaacctc agcaaatata tgagtttgta aaaagaccat
    11281 gaaataacag acaatggact cccttagcgg gccaggttgt gggccgggtc caggggccat
    11341 tccaaagggg agacgactca atggtgtaag acgacattgt ggaatagcaa gggcagttcc
    11401 tcgccttagg ttgtaaaggg aggtcttact acctccatat acgaacacac cggcgaccca
    11461 agttccttcg tcggtagtcc tttctacgtg actcctagcc aggagagctc ttaaaccttc
    11521 tgcaatgttc tcaaatttcg ggttggaacc tccttgacca cgatgctttc caaaccaccc
    11581 tccttttttg cgcctgcctc catcaccct
//
