LOCUS       sequence-240384-       11022 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the ZM197M HIV-1 isolate; 
            C-terminal truncation; QES mutant with enhanced binding to PG16.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11022)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 11022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-ZM197M-QES.c10-754*
FEATURES             Location/Qualifiers
     source          1..11022
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      578..2367
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     2927..2949
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     polyA_signal    3092..3226
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3129..3148)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3183..3202
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5571..5595)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(5592..5795)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(5621..5641)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5796..6175
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     polyA_signal    6829..6877
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     6902..6921
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(6919..7956)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     promoter        complement(7999..8144)
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     rep_origin      complement(8575..9163)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8655..8674)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9334..10125,10126..10194))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPXAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9957..9976
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10195..10299)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10367..10385
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cctggcccgc taagggagtc cattgtctgt tatttcatgg tctttttaca aactcatata
       61 tttgctgagg ttttgaagga tgcgattaag gaccttgtta tgacaaagcc cgctcctacc
      121 tgcaatatca gggtgactgt gtgcagcttt gacgatggag tagatttgcc tccctggttt
      181 ccacctatgg tggaaggggc tgccgcggag ggtgatgacg gagatgacgg agatgaagga
      241 ggtgatggag atgagggtga ggaagggcag gagtgatgta acttgttagg agacgccctc
      301 aatcgtatta aaagccgtgt attcccccgc actaaagaat aaatccccag tagacatcat
      361 gcgtgctgtt ggtgtatttc tggccatctg tcttgtcacc attttcgtcc tcccaacatg
      421 gggcaattgg gcatacccat gttgtcacgt cactcagctc cgcgctcaac accttctcgc
      481 gttggaaaac attagcgaca tttacctggt gagcaatcag acatgcgacg gctttagcct
      541 ggcctcctta aattcaccta agaatgggag caaccagcag gaaaaggaca agcagcgaaa
      601 attcacgccc ccttgggagg tggcggcata tgcaaaggat agcactccca ctctactact
      661 gggtatcata tgctgactgt atatgcatga ggatagcata tgctacccgg atacagatta
      721 ggatagcata tactacccag atatagatta ggatagcata tgctacccag atatagatta
      781 ggatagccta tgctacccag atataaatta ggatagcata tactacccag atatagatta
      841 ggatagcata tgctacccag atatagatta ggatagccta tgctacccag atatagatta
      901 ggatagcata tgctacccag atatagatta ggatagcata tgctatccag atatttgggt
      961 agtatatgct acccagatat aaattaggat agcatatact accctaatct ctattaggat
     1021 agcatatgct acccggatac agattaggat agcatatact acccagatat agattaggat
     1081 agcatatgct acccagatat agattaggat agcctatgct acccagatat aaattaggat
     1141 agcatatact acccagatat agattaggat agcatatgct acccagatat agattaggat
     1201 agcctatgct acccagatat agattaggat agcatatgct atccagatat ttgggtagta
     1261 tatgctaccc atggcaacat tagcccaccg tgctctcagc gacctcgtga atatgaggac
     1321 caacaaccct gtgcttggcg ctcaggcgca agtgtgtgta atttgtcctc cagatcgcag
     1381 caatcgcgcc cctatcttgg cccgcccacc tacttatgca ggtattcccc ggggtgccat
     1441 tagtggtttt gtgggcaagt ggtttgaccg cagtggttag cggggttaca atcagccaag
     1501 ttattacacc cttattttac agtccaaaac cgcagggcgg cgtgtggggg ctgacgcgtg
     1561 cccccactcc acaatttcaa aaaaaagagt ggccacttgt ctttgtttat gggccccatt
     1621 ggcgtggagc cccgtttaat tttcgggggt gttagagaca accagtggag tccgctgctg
     1681 tcggcgtcca ctctctttcc ccttgttaca aatagagtgt aacaacatgg ttcacctgtc
     1741 ttggtccctg cctgggacac atcttaataa ccccagtatc atattgcact aggattatgt
     1801 gttgcccata gccataaatt cgtgtgagat ggacatccag tctttacggc ttgtccccac
     1861 cccatggatt tctattgtta aagatattca gaatgtttca ttcctacact agtatttatt
     1921 gcccaagggg tttgtgaggg ttatattggt gtcatagcac aatgccacca ctgaaccccc
     1981 cgtccaaatt ttattctggg ggcgtcacct gaaaccttgt tttcgagcac ctcacataca
     2041 ccttactgtt cacaactcag cagttattct attagctaaa cgaaggagaa tgaagaagca
     2101 ggcgaagatt caggagagtt cactgcccgc tccttgatct tcagccactg cccttgtgac
     2161 taaaatggtt cactaccctc gtggaatcct gaccccatgt aaataaaacc gtgacagctc
     2221 atggggtggg agatatcgct gttccttagg acccttttac taaccctaat tcgatagcat
     2281 atgcttcccg ttgggtaaca tatgctattg aattagggtt agtctggata gtatatacta
     2341 ctacccggga agcatatgct acccgtttag ggttaacaag ggggccttat aaacactatt
     2401 gctaatgccc tcttgagggt ccgcttatcg gtagctacac aggcccctct gattgacgtt
     2461 ggtgtagcct cccgtagtct tcctgggccc ctgggaggta catgtccccc agcattggtg
     2521 taagagcttc agccaagagt tacacataaa ggcaatgttg tgttgcagtc cacagactgc
     2581 aaagtctgct ccaggatgaa agccactcag tgttggcaaa tgtgcacatc catttataag
     2641 gatgtcaact acagtcagag aacccctttg tgtttggtcc ccccccgtgt cacatgtgga
     2701 acagggccca gttggcaagt tgtaccaacc aactgaaggg attacatgca ctgccccgcg
     2761 aagaaggggc agagatgtcg tagtcaggtt tagttcgtcc ggggcgggga tcgatcctct
     2821 agagtcgacc tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     2881 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     2941 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agccggatca taatcagcca
     3001 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
     3061 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
     3121 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     3181 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatccggc cttgccggcc
     3241 tcgagttatc acaaaaagcc gctaaccaat ctcgttgacc tattcttatc ttgctcgcca
     3301 ccctcttcct caatacctcc tggtctgtcg ggtccccgtg gattgggaat cagcgtttgg
     3361 aaggagagcg gggaataacc ctgccgtacc cgattgacca ctgacaatac agcaaaaata
     3421 atcctcaaac caatcaaacc cccaacgatc atgataaata tcttaatgta ccagagccac
     3481 ttcgttatat caaaccaact ccacaggctg ttccacttat ccaaagcaag gagatcattc
     3541 tcgttcttct cctgctgatt ctgagatact tcgagaagtt ggtagatgac ctgggtgtag
     3601 ttatctattt cccgatccca ctgcatccat gtcatgttgt cccatatttc gtctttgctt
     3661 ttgttagacc aactggtgtt ccagggtacc gctgtggtac agatcagttt accactgcac
     3721 ccccacaaac caagcaattg ctgatcctta aggtagcgct cgatagccag tacccgagtt
     3781 tgcagttgtt taattcccca tactgtaagt tgcaacatat gctgttgtgc ctcgatagcg
     3841 cgaagcaggt tagattgttg ctgaacaata ccactcaaca actgtcgtgc ctggacagta
     3901 agcatcacac tagcagcgcc catagttgaa cccgctgcgc cgagaaagcc caatattacc
     3961 gcgccgatcc ccgcggctct tttctctctt tcgacgaccc gccgtttcgc gctagtaggt
     4021 gcaatcccaa ggggtttgat ttcaaccacc ttgtatttat acagctcgga tcgccaatta
     4081 tctttcatgt tcccgccccc tgggcgaaag gtctctatcc ctgcgctttt attcccaccg
     4141 tctcgtgtga gcaagagccc ggtgatgtta cttttgcatg ttatgatccc ggcaatcggt
     4201 gaggcgtaca ttgcccgccc cacttcctgc cacatattga tgatttgttt tatgcggcac
     4261 gggagcgtga tggttgcatt agaagtagag ttgctcgttc cgtctgtatc gttcagcaga
     4321 gaagagttga agaggccaga cgtattacaa tagaagaact cgccgcgaca attaaaagaa
     4381 tgggtagtga tctcaaggtc gccgccagcg gaagattcaa acttgatggt agcgttattg
     4441 aagtgttcct tcaatttctt ctcaatcctt ttaagcgtcg tggtccaatt agactttgaa
     4501 agatcgcaat gcgcctggcg aatgtcacct atgatttccc ctgtagcgaa gaaagtttgc
     4561 ccgggaccga ttctgacgct tttcctcgtg ttattattgg gcctaacgca ttcaatctcg
     4621 actgactcgt tcaaatgcac tataattgtt ttagtgttgt cagtcaggtt ctcagaccgt
     4681 atgatgattt cctcctcggc aagagaaccg ttaagaagca gttgtgtaga catagttggt
     4741 tttatcccgt gcgtgcactg aaccgtactg acgttatgac atggtccggt accgttgaat
     4801 gttttattgt tacatttaag tatcgcatag cctgctggag cgcagtagtg gatcggaatt
     4861 gggtcaaaag acatttttgg acaggcttgg gtaattgtgc tcgtgttgca gctaatcaat
     4921 cggtactctt tgtaagagct gtttttagag ctggagtttt tctcctcctc cagagggacc
     4981 acatcaagtt tatagaacag tgcgcgttgc ttagtctttc tgtctcggac ttcagttgtg
     5041 atattaaagg tacaatcttt aatttcccct ttcatttcgg tatcgttact tgtcgcattt
     5101 cggttgtctg ttgaagtcgt gtttgtgtta gtcgcattct ttgtcgtatt acttgtagcg
     5161 tcgctgcaat tcagagtcac gcacaatggg gtcagtttga cgcatggttt caagctttgg
     5221 tcccacaaac taattatgtc ttcgtgcatt tggtctgcca tgtcgttctt ccacatattg
     5281 aaattttccg tcacgttacc caagggtatc tcttggggat tcggatctgt gggtacgcaa
     5341 gcatgggtgg cccacacgtt atgtacttct ttttcgtagg ctttcgcatc gctggcgcag
     5401 aagagagttg ctttagcctc tttccacacc ggaacaccgt aatacaccgt aacccacagt
     5461 tgttccgcca agactgacgc caccaacata ccgagaagat acagcgttgc cagcggttgg
     5521 agactcccca tgggcatggt ggcgctagca gctggtaccc agcttctaga gatctgacgg
     5581 ttcactaaac gagctctgct tatatagacc tcccaccgta cacgcctacc gcccatttgc
     5641 gtcaacgggg cggggttatt acgacatttt ggaaagtccc gttgattttg gtgccaaaac
     5701 aaactcccat tgacgtcaat ggggtggaga cttggaaatc cccgtgagtc aaaccgctat
     5761 ccacgcccat tggtgtactg ccaaaaccgc atcaccatgg taatagcgat gactaatacg
     5821 tagatgtact gccaagtagg aaagtcccgt aaggtcatgt actgggcata atgccaggcg
     5881 ggccatttac cgtcattgac gtcaataggg ggcggacttg gcatatgata cacttgatgt
     5941 actgccaagt gggcagttta ccgtaaatac tccacccatt gacgtcaatg gaaagtccct
     6001 attggcgtta ctatgggaac atacgtcatt attgacgtca atgggcgggg gtcgttgggc
     6061 ggtcagccag gcgggccatt taccgtaagt tatgtaacgc ggaactccat atatgggcta
     6121 tgaactaatg accccgtaat tgattactat taataactag tcaataatca atgtcaacat
     6181 ggcggtcata ttggacatga gccaatataa atgtacatat tatgatatag atacaacgta
     6241 tgcaatggcc aatagccaat attgatttat gctatataac caatgactaa tatggctaat
     6301 tgccaatatt gattcaatgt atagatcttc catacctacc agttctgcgc ctgcagcaat
     6361 ggcaacaacg ttgcccggat ctgcgatgat aagctgtcaa acatgagaat tggtcgacta
     6421 gcttggcacg ccagaaatcc gcgcggtggt ttttgggggt cgggggtgtt tggcagccac
     6481 agacgcccgg tgttcgtgtc gcgccagtac atgcggtcca tgcccaggcc atccaaaaac
     6541 catgggtctg tctgctcagt ccagtcgtgg acctgacccc acgcaacgcc caaaataata
     6601 acccccacga accataaacc attccccatg ggggaccccg tccctaaccc acggggccag
     6661 tggctatggc agggcctgcc gccccgacgt tggctgcgag ccctgggcct tcacccgaac
     6721 ttggggggtg gggtggggaa aaggaagaaa cgcgggcgta ttggccccaa tggggtctcg
     6781 gtggggtatc gacagagtgc cagccctggg accgaacccc gcgtttatga acaaacgacc
     6841 caacacccgt gcgttttatt ctgtcttttt attgccgtca tagcgcgggt tccttccggt
     6901 attgtctcct tccgtgtttc agttagcctc ccccatctcc cgatccggac gagtgctggg
     6961 gcgtcggttt ccactatcgg cgagtacttc tacacagcca tcggtccaga cggccgcgct
     7021 tctgcgggcg atttgtgtac gcccgacagt cccggctccg gatcggacga ttgcgtcgca
     7081 tcgaccctgc gcccaagctg catcatcgaa attgccgtca accaagctct gatagagttg
     7141 gtcaagacca atgcggagca tatacgcccg gagccgcggc gatcctgcaa gctccggatg
     7201 cctccgctcg aagtagcgcg tctgctgctc catacaagcc aaccacggcc tccagaagaa
     7261 gatgttggcg acctcgtatt gggaatcccc gaacatcgcc tcgctccagt caatgaccgc
     7321 tgttatgcgg ccattgtccg tcaggacatt gttggagccg aaatccgcgt gcacgaggtg
     7381 ccggacttcg gggcagtcct cggcccaaag catcagctca tcgagagcct gcgcgacgga
     7441 cgcactgacg gtgtcgtcca tcacagtttg ccagtgatac acatggggat cagcaatcgc
     7501 gcatatgaaa tcacgccatg tagtgtattg accgattcct tgcggtccga atgggccgaa
     7561 cccgctcgtc tggctaagat cggccgcagc gatcgcatcc atggcctccg cgaccggctg
     7621 cagaacagcg ggcagttcgg tttcaggcag gtcttgcaac gtgacaccct gtgcacggcg
     7681 ggagatgcaa taggtcaggc tctcgctgaa ttccccaatg tcaagcactt ccggaatcgg
     7741 gagcgcggcc gatgcaaagt gccgataaac ataacgatct ttgtagaaac catcggcgca
     7801 gctatttacc cgcaggacat atccacgccc tcctacatcg aagctgaaag cacgagattc
     7861 ttcgccctcc gagagctgca tcaggtcgga gacgctgtcg aacttttcga tcagaaactt
     7921 ctcgacagac gtcgcggtga gttcaggctt tttcatatct cattgccccc cgggatctgc
     7981 ggcacgctgt tgacgctgtt aagcgggtcg ctgcagggtc gctcggtgtt cgaggccaca
     8041 cgcgtcacct taatatgcga agtggacctc ggaccgcgcc gccccgactg catctgcgtg
     8101 ttcgaattcg ccaatgacaa gacgctgggc ggggtttgtg tcatcataga actaaagaca
     8161 tgcaaatata tttcttccgg ggacaccgcc agcaaacgcg agcaacgggc cacggggatg
     8221 aagcagggcg gcacctcgct aacggattca ccactccaag aattggagcc aatcaattct
     8281 tgcggagaac tgtgaatgcg caaaccaacc cttggcagaa catatccatc gcgtccgcca
     8341 tctccagcag ccgcacgcgg cgcatctcgg ggccgacgcg ctgggctacg tcttgctggc
     8401 gttcgcgacg cgaggctgga tggccttccc cattatgatt cttctcgctt ccggcggcat
     8461 cgggatgccc gcgttgcagg ccatgctgtc caggcaggta gatgacgacc atcagggaca
     8521 gcttcaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     8581 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     8641 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     8701 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     8761 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     8821 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     8881 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     8941 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     9001 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     9061 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     9121 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     9181 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     9241 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     9301 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     9361 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     9421 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     9481 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     9541 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     9601 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgctr caggcatcgt
     9661 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     9721 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     9781 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     9841 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     9901 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa
     9961 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
    10021 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
    10081 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
    10141 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
    10201 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
    10261 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
    10321 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
    10381 gaggcccttt cgtcttcaag aattctcatg tttgacagct tatcatcgat aagctgatcc
    10441 tcacaggccg cacccagctt ttcttccgtt gccccagtag catctctgtc tggtgacctt
    10501 gaagaggaag aggaggggtc ccgagaatcc ccatccctac cgtccagcaa aaagggggac
    10561 gaggaatttg aggcctggct tgaggctcag gacgcaaatc ttgaggatgt tcagcgggag
    10621 ttttccgggc tgcgagtaat tggtgatgag gacgaggatg gttcggagga tggggaattt
    10681 tcagacctgg atctgtctga cagcgaccat gaaggggatg agggtggggg ggctgttgga
    10741 gggggcagga gtctgcactc cctgtattca ctgagcgtcg tctaataaag atgtctattg
    10801 atctctttta gtgtgaatca tgtctgacga ggggccaggt acaggacctg gaaatggcct
    10861 aggagagaag ggagacacat ctggaccaga aggctccggc ggcagtggac ctcaaagaag
    10921 agggggtgat aaccatggac gaggacgggg aagaggacga ggacgaggag gcggaagacc
    10981 aggagccccg ggcggctcag gatcagggcc aagacataga ga
//
