LOCUS       sequence-240395-        4628 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase reporter driven by the Sox2 enhancer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4628)
  AUTHORS   Adachi K, Kopp W, Wu G, Heising S, Greber B, Stehling M, 
            Arauzo-Bravo MJ, Boerno ST, Timmermann B, Vingron M, Scholer HR
  TITLE     Esrrb Unlocks Silenced Enhancers for Reprogramming to Naive 
            Pluripotency.
  JOURNAL   Cell Stem Cell. 2018 Aug 2;23(2):266-275.e6. doi: 
            10.1016/j.stem.2018.05.020. Epub 2018 Jun 14.
  PUBMED    29910149
REFERENCE   2  (bases 1 to 4628)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2018.05.020"; journalName: "Cell Stem Cell"; date: 
            "2018-08-2- 2"; volume: "23"; issue: "2"; pages: "266-275.e6"
COMMENT     Sequence Label: KA1874_pGL4-UBCmin-luc2/Sox2_SRR1
FEATURES             Location/Qualifiers
     source          1..4628
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    186..234
                     /note="synthetic polyadenylation signal"
     misc_feature    248..339
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     288..307
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     regulatory      521..530
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             527..2179
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    2223..2344
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2234..2253)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2288..2307
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2890..2907)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3061..3649)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3141..3160)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3849..4628,1..12,13..81))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 cgtttcgggg tgtgcaaaaa caggcaagca aaatgccgca aagaagggaa tgagtgcgac
       61 acgaaaatgt tggatgctca tactcgtcct ttttcaatat tattgaagca tttatcaggg
      121 ttactagtac gtctctcaag gataagtaag taatattaag gtacgggagg tattggacag
      181 gccgcaataa aatatcttta ttttcattac atctgtgtgt tggttttttg tgtgaatcga
      241 tagtactaac atacgctctc catcaaaaca aaacgaaaca aaacaaacta gcaaaatagg
      301 ctgtccccag tgcaagtgca ggtgccagaa catttctctg gcctaactgg ccggtacctg
      361 agctcgctag cctcgaggat ggggcggtga acgccgatga ttatataagg acgcgccggg
      421 tgtggcacag ctagttccgt cgcagccggg atttgaattc ctcgactcta gaggatctgg
      481 cctcggcggc caagcttggc aatccggtac tgttggtaaa gccaccatgg aagatgccaa
      541 aaacattaag aagggcccag cgccattcta cccactcgaa gacgggaccg ccggcgagca
      601 gctgcacaaa gccatgaagc gctacgccct ggtgcccggc accatcgcct ttaccgacgc
      661 acatatcgag gtggacatta cctacgccga gtacttcgag atgagcgttc ggctggcaga
      721 agctatgaag cgctatgggc tgaatacaaa ccatcggatc gtggtgtgca gcgagaatag
      781 cttgcagttc ttcatgcccg tgttgggtgc cctgttcatc ggtgtggctg tggccccagc
      841 taacgacatc tacaacgagc gcgagctgct gaacagcatg ggcatcagcc agcccaccgt
      901 cgtattcgtg agcaagaaag ggctgcaaaa gatcctcaac gtgcaaaaga agctaccgat
      961 catacaaaag atcatcatca tggatagcaa gaccgactac cagggcttcc aaagcatgta
     1021 caccttcgtg acttcccatt tgccacccgg cttcaacgag tacgacttcg tgcccgagag
     1081 cttcgaccgg gacaaaacca tcgccctgat catgaacagt agtggcagta ccggattgcc
     1141 caagggcgta gccctaccgc accgcaccgc ttgtgtccga ttcagtcatg cccgcgaccc
     1201 catcttcggc aaccagatca tccccgacac cgctatcctc agcgtggtgc catttcacca
     1261 cggcttcggc atgttcacca cgctgggcta cttgatctgc ggctttcggg tcgtgctcat
     1321 gtaccgcttc gaggaggagc tattcttgcg cagcttgcaa gactataaga ttcaatctgc
     1381 cctgctggtg cccacactat ttagcttctt cgctaagagc actctcatcg acaagtacga
     1441 cctaagcaac ttgcacgaga tcgccagcgg cggggcgccg ctcagcaagg aggtaggtga
     1501 ggccgtggcc aaacgcttcc acctaccagg catccgccag ggctacggcc tgacagaaac
     1561 aaccagcgcc attctgatca cccccgaagg ggacgacaag cctggcgcag taggcaaggt
     1621 ggtgcccttc ttcgaggcta aggtggtgga cttggacacc ggtaagacac tgggtgtgaa
     1681 ccagcgcggc gagctgtgcg tccgtggccc catgatcatg agcggctacg ttaacaaccc
     1741 cgaggctaca aacgctctca tcgacaagga cggctggctg cacagcggcg acatcgccta
     1801 ctgggacgag gacgagcact tcttcatcgt ggaccggctg aagagcctga tcaaatacaa
     1861 gggctaccag gtagccccag ccgaactgga gagcatcctg ctgcaacacc ccaacatctt
     1921 cgacgccggg gtcgccggcc tgcccgacga cgatgccggc gagctgcccg ccgcagtcgt
     1981 cgtgctggaa cacggtaaaa ccatgaccga gaaggagatc gtggactatg tggccagcca
     2041 ggttacaacc gccaagaagc tgcgcggtgg tgttgtgttc gtggacgagg tgcctaaagg
     2101 actgaccggc aagttggacg cccgcaagat ccgcgagatt ctcattaagg ccaagaaggg
     2161 cggcaagatc gccgtgtaat aattctagag tcggggcggc cggccgcttc gagcagacat
     2221 gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt
     2281 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
     2341 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
     2401 tttttaaagc aagtaaaacc tctacaaatg tggtaaaatc gataaggatc ctgccgttcg
     2461 ccttcatttc cataaggaga ttaggagagg aggggaaccc actcaaatgc agatgcagga
     2521 gcgaagcgtt tttaacaagc atcataatag taagatgctt ggctagttct cgctaattac
     2581 tgcaacttaa acctctattt gccactaaga agaaaaaaat aagtctacag tccgcacctc
     2641 cacaacattg tcagttggct tcagatataa tactcgtcga ggagagaaaa atgtaaaaga
     2701 tttttaaata aaattgggtt atgcttggca gttaaggctt ctggcttggg tcgaccgatg
     2761 cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc
     2821 gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc
     2881 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     2941 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     3001 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     3061 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     3121 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     3181 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     3241 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     3301 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     3361 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     3421 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     3481 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     3541 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     3601 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     3661 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     3721 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     3781 atcaatctaa agtatatatg agtaaacttg gtctgacagc ggccgcaaat gctaaaccac
     3841 tgcagtggtt accagtgctt gatcagtgag gcaccgatct cagcgatctg cctatttcgt
     3901 tcgtccatag tggcctgact ccccgtcgtg tagatcacta cgattcgtga gggcttacca
     3961 tcaggcccca gcgcagcaat gatgccgcga gagccgcgtt caccggcccc cgatttgtca
     4021 gcaatgaacc agccagcagg gagggccgag cgaagaagtg gtcctgctac tttgtccgcc
     4081 tccatccagt ctatgagctg ctgtcgtgat gctagagtaa gaagttcgcc agtgagtagt
     4141 ttccgaagag ttgtggccat tgctactggc atcgtggtat cacgctcgtc gttcggtatg
     4201 gcttcgttca actctggttc ccagcggtca agccgggtca catgatcacc catattatga
     4261 agaaatgcag tcagctcctt agggcctccg atcgttgtca gaagtaagtt ggccgcggtg
     4321 ttgtcgctca tggtaatggc agcactacac aattctctta ccgtcatgcc atccgtaaga
     4381 tgcttttccg tgaccggcga gtactcaacc aagtcgtttt gtgagtagtg tatacggcga
     4441 ccaagctgct cttgcccggc gtctatacgg gacaacaccg cgccacatag cagtactttg
     4501 aaagtgctca tcatcgggaa tcgttcttcg gggcggaaag actcaaggat cttgccgcta
     4561 ttgagatcca gttcgatata gcccactctt gcacccagtt gatcttcagc atcttttact
     4621 ttcaccag
//
