LOCUS       sequence-240402-        5782 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BglFusion compatible IPTG inducible integration vector for 
            Streptococcus pneumoniae (pPEPZ Backbone).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5782)
  AUTHORS   Keller LE, Rueff AS, Kurushima J, Veening JW
  TITLE     Three New Integration Vectors and Fluorescent Proteins for Use in 
            the Opportunistic Human Pathogen Streptococcus pneumoniae.
  JOURNAL   Genes (Basel). 2019 May 22;10(5). pii: genes10050394. doi: 
            10.3390/genes10050394.
  PUBMED    31121970
REFERENCE   2  (bases 1 to 5782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            (Basel)."; date: "2019-05-22"; volume: "10(5). pii: genes10050394. 
            doi"; pages: " 10.3390/genes10050394"
COMMENT     Sequence Label: pASR130
FEATURES             Location/Qualifiers
     source          1..5782
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      18..89
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      105..132
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     protein_bind    229..248
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     rep_origin      1379..1967
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1868..1887
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2055..2126
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2127..2195,2196..2987)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2345..2364)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     mobile_element  4760..5527
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
ORIGIN
        1 acacgctagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
       61 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg
      121 ggcctttctg cgtttataga attcactagt aattgtgagg cggataacaa ttctcactca
      181 ttctacagtt tattcttgac attgcactgt ccccctggta taataactaa ttgtgagcgc
      241 tcacaattaa gatctaaata aataaggagg aaaaataatg ccggatccct ccagtaactc
      301 gagaaaaaaa aaccgcgccc ctgacagggc gcggtttttt tttcgtaatt tattactgaa
      361 caagagggca atcatatctg gaataatatg cttaaaaaga gaaggaaaat tggtgcaaat
      421 tcattttcag actatcttcg tggaagtatt catcaaaata gacaaaaata aatttggata
      481 aatcgaactc actattcagg aggcatatga gcaattcgaa aaagaaaagt gtcaaattga
      541 gcctatagga gtagaagtga aatagtaagt cctgcatagt ggatgagaga aaagttctcc
      601 ttgaagtttt cctgaactat cagtcgcatg tcaaacgata tgtagggtaa tgtgagaggg
      661 gatagcgagt agtttttggt tattttatca aaaaacttat attttattat accgaatgat
      721 aaaatataat aaaaatgata gaataaggaa aaaacatgaa tgtcaaaaag ataatgtcaa
      781 tttttcaatc cttttatgtt gatgtcagta ttgaggaact gactttgact ttaccaatca
      841 gttttgtaaa aaggtttgag tatactcaaa tgacttttca taaggaatca tttttattga
      901 ttaaagaaaa gagaaggggg agtttgagtt catttgttac tcaggctcgc actatgggtg
      961 aaaaagccaa tatggatgtt gttttggtgt tttcgaagtt atcagacagt gaaaaaaagc
     1021 aattacttca agctagagtt ccgtttgtag actttaaggg aaacctcttc ttccctccat
     1081 tgggactagt actcaatgcg aatgatactg aagtccctaa ggaattaaca cctagcgaac
     1141 aattaacgtg gattgccttt ttattgacaa aaggtcaaaa agtagtagat gttgatttgc
     1201 tttcacaagt cactggactt ccaaactcaa caatttatag gtgtttgagg acttttaaag
     1261 ctttatattg gttaaacaag caaaataagc tttacacata tacggtgtca aagaaagaat
     1321 tattcttaaa atccgtgtca tgttggtacc accccgtaga aaagatcaaa ggatcttctt
     1381 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctggtag
     1441 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     1501 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     1561 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     1621 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     1681 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     1741 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     1801 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     1861 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     1921 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     1981 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     2041 tatcccgcgg ccgccattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     2101 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     2161 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     2221 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     2281 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     2341 ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc
     2401 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     2461 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     2521 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     2581 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     2641 caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat
     2701 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     2761 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     2821 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     2881 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     2941 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     3001 tttactcata gcggccgcca ataaattgct tccttgtttt gtcataattt ttttatttaa
     3061 aaaattatga caaaagtgtg ctattctttt tatgagaggt gtatgaatat gataaatgta
     3121 tgtgataaat gtatgtgatg ttggaaaaag aataaaagaa cttagaatat cttcaaatct
     3181 tactcaagat aagattgctg aatatttgtc tttatatttg tctttgaatc aaagcatgat
     3241 tgccaaaatg gaaaaaggtg aaaggaatat cacgaatgga tttaagtaat aaagcttcaa
     3301 atcttagaaa aaagttggga gctgatggtg aagcgccgat agatattttt aaattggtac
     3361 aaaagataga aaatttgacg ctggtatttt atggactcgg aaagaatatt agcggagtct
     3421 gttataaagg aactcagttc agtctcattg cagtcaattc agacatgtca ttaggaaggt
     3481 aaagattttc tttagcacat gaactgtatc atctttatta tgatgaggtg aagaagagtt
     3541 cagtcagtct tatcttgatt ggtgaaggag atgaaactga aagaaaagcg gatcagcttg
     3601 cttcttattt tttaattttc ccatcttcac tgtataggat ggttgaggaa atcagagaaa
     3661 atgccaatag aactcatctt gaagtagaag atattataaa attgggtcag ttttatggta
     3721 tcagtcataa agctatgtta tatagattga ggaatgatgg ataccttgat gcagaagaaa
     3781 ttaaaaatat ggatattagt gttatagaga cagcttcaag attaggctat gatacaagtt
     3841 tatatcgtcc tttgtcagaa agtaaaaagg aaatggtatt aggataatat attaattcaa
     3901 ctgaacaact tttagaaaat aacagaattt cgcaagggaa gtatgaggaa ctgttactag
     3961 atgctttcag atatgatatt gtatatgggc tagatgaaga ggggggagtt gtcgttgaaa
     4021 agcttctagc aaaaaactgg acggagtccc gtccagaaaa gtaagcactt gttattgcaa
     4081 taaaattagc ttttacgacg cgcatagacg gagtgcgtgt acgtgattgt gccttgaatg
     4141 gtttcggatg agacaacctc gaattctgct gcgttaagca ctgggaagaa ggcgtcaccc
     4201 tcaaaggttt gatgtacctc agatagaaag acgccgttgg catgcggtag cgccagcgca
     4261 tatacctcgg ctccgccggc tacgtagagt tctttgccgt gttcggctgc gatggcgata
     4321 gcctgtgaca gcgttgaaac aactgcacaa ccaggagcgc tataaccagc ttggcgcgag
     4381 agcaccactg tgtggcggtt tggtaagggc ttgcctatgg actcaaatgt cttgcggccc
     4441 ataacgacca ctttgctctc ggtgagcctg cgaaaaatct tctgctcacc tgggattttc
     4501 caggggatat cgggaccatt gccaataacc cgattggcac ccatggcagc gaccagataa
     4561 atgcggaccg attccgggtt cattaatttt cctcctacat gatttcacct cgttgattat
     4621 gttcatataa agtttatatt gatactcaat ttacttaccc tagattggac atatacttaa
     4681 attactgttc aataaagctg accgttagcg tttaagtaca tcctttcaca atttgtctac
     4741 agaacagtac tggctattag gtaatgactc caacttattg atagtgtttt atgttcagat
     4801 aatgcccgat gactttgtca tgcagctcca ccgattttga gaacgacagc gacttccgtc
     4861 ccagccgtgc caggtgctgc ctcagattca ggttatgccg ctcaattcgc tgcgtatatc
     4921 gcttgctgat tacgtgcagc tttcccttca ggcgggattc atacagcggc cagccatccg
     4981 tcatccatat caccacgtca aagggtgaca gcaggctcat aagacgcccc agcgtcgcca
     5041 tagtgcgttc accgaatacg tgcgcaacaa ccgtcttccg gagactgtca tacgcgtaaa
     5101 acagccagcg ctggcgcgat ttagccccga catagcccca ctgttcgtcc atttccgcgc
     5161 agacgatgac gtcactgccc ggctgtatgc gcgaggttac cgactgcggc ctgagttttt
     5221 taagtgacgt aaaatcgtgt tgaggccaac gcccataatg cgggctgttg cccggcatcc
     5281 aacgccattc atggccatat caatgatttt ctggtgcgta ccgggttgag aagcggtgta
     5341 agtgaactgc agttgccatg ttttacggca gtgagagcag agatagcgct gatgtccggc
     5401 ggtgcttttg ccgttacgca ccaccccgtc agtagctgaa caggagggac agctgataga
     5461 aacagaagcc actggagcac ctcaaaaaca ccatcataca ctaaatcagt aagttggcag
     5521 catcacctgg ctattatatc agccttctta ttcaatgtca agaataaact gttctatgta
     5581 aacctattca ttgttttgca tgcggcaaag cactcaaaag tttaccttat gggtgctttt
     5641 ttcgtgcttt tttgaaaaga caaaaaaaag aaccttgcca agcaagattc ttttgatagg
     5701 gtggtctccg atcgtgtact gcagaaaact agtcgcaaaa aaccccgctt cggcggggtt
     5761 ttttcgctct agacggtgat ca
//
