LOCUS       sequence-240415-       11776 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the AD8 HIV-1 isolate; QES
            mutant for enhanced presentation of quaternary epitopes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11776)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 11776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-AD8-QES.i08.c04
FEATURES             Location/Qualifiers
     source          1..11776
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(642..660)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        728..832
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(833..901,902..1693)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1051..1070)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1864..2452
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2353..2372
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2883..3028
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3071..4108
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4106..4125)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4150..4198
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4852..5231
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5232..5435
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5386..5406
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5432..5456
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     polyA_signal    8062..8196
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8086..8105)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8140..8159
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(8339..8361)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8921..10710
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 accatctcta tgtcttggcc ctgatcctga gccgcccggg gctcctggtc ttccgcctcc
       61 tcgtcctcgt cctcttcccc gtcctcgtcc atggttatca ccccctcttc tttgaggtcc
      121 actgccgccg gagccttctg gtccagatgt gtctcccttc tctcctaggc catttccagg
      181 tcctgtacct ggcccctcgt cagacatgat tcacactaaa agagatcaat agacatcttt
      241 attagacgac gctcagtgaa tacagggagt gcagactcct gccccctcca acagcccccc
      301 caccctcatc cccttcatgg tcgctgtcag acagatccag gtctgaaaat tccccatcct
      361 ccgaaccatc ctcgtcctca tcaccaatta ctcgcagccc ggaaaactcc cgctgaacat
      421 cctcaagatt tgcgtcctga gcctcaagcc aggcctcaaa ttcctcgtcc ccctttttgc
      481 tggacggtag ggatggggat tctcgggacc cctcctcttc ctcttcaagg tcaccagaca
      541 gagatgctac tggggcaacg gaagaaaagc tgggtgcggc ctgtgaggat cagcttatcg
      601 atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt
      661 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
      721 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
      781 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
      841 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
      901 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
      961 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     1021 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga
     1081 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     1141 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     1201 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     1261 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     1321 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc
     1381 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     1441 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     1501 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     1561 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     1621 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     1681 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     1741 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     1801 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     1861 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     1921 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     1981 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     2041 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     2101 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     2161 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     2221 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     2281 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     2341 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     2401 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     2461 caacgcggcc tttttacggt tcctggcctt ttgctggcct tgaagctgtc cctgatggtc
     2521 gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc
     2581 gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta
     2641 gcccagcgcg tcggccccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg
     2701 gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct
     2761 ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ctgcttcatc cccgtggccc
     2821 gttgctcgcg tttgctggcg gtgtccccgg aagaaatata tttgcatgtc tttagttcta
     2881 tgatgacaca aaccccgccc agcgtcttgt cattggcgaa ttcgaacacg cagatgcagt
     2941 cggggcggcg cggtccgagg tccacttcgc atattaaggt gacgcgtgtg gcctcgaaca
     3001 ccgagcgacc ctgcagcgac ccgcttaaca gcgtcaacag cgtgccgcag atcccggggg
     3061 gcaatgagat atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga
     3121 aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt
     3181 cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt
     3241 ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt
     3301 gcttgacatt ggggaattca gcgagagcct gacctattgc atctcccgcc gtgcacaggg
     3361 tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga
     3421 ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg
     3481 accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc
     3541 ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc
     3601 tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc
     3661 ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg
     3721 gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc
     3781 gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc
     3841 aggatcgccg cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag
     3901 cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt
     3961 ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg
     4021 gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc
     4081 ggatcgggag atgggggagg ctaactgaaa cacggaagga gacaataccg gaaggaaccc
     4141 gcgctatgac ggcaataaaa agacagaata aaacgcacgg gtgttgggtc gtttgttcat
     4201 aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg
     4261 ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc
     4321 ccagggctcg cagccaacgt cggggcggca ggccctgcca tagccactgg ccccgtgggt
     4381 tagggacggg gtcccccatg gggaatggtt tatggttcgt gggggttatt attttgggcg
     4441 ttgcgtgggg tcaggtccac gactggactg agcagacaga cccatggttt ttggatggcc
     4501 tgggcatgga ccgcatgtac tggcgcgaca cgaacaccgg gcgtctgtgg ctgccaaaca
     4561 cccccgaccc ccaaaaacca ccgcgcggat ttctggcgtg ccaagctagt cgaccaattc
     4621 tcatgtttga cagcttatca tcgcagatcc gggcaacgtt gttgccattg ctgcaggcgc
     4681 agaactggta ggtatggaag atctatacat tgaatcaata ttggcaatta gccatattag
     4741 tcattggtta tatagcataa atcaatattg gctattggcc attgcatacg ttgtatctat
     4801 atcataatat gtacatttat attggctcat gtccaatatg accgccatgt tgacattgat
     4861 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
     4921 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
     4981 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
     5041 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
     5101 atatgccaag tccgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
     5161 cccagtacat gaccttacgg gactttccta cttggcagta catctacgta ttagtcatcg
     5221 ctattaccat ggtgatgcgg ttttggcagt acaccaatgg gcgtggatag cggtttgact
     5281 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
     5341 atcaacggga ctttccaaaa tgtcgtaata accccgcccc gttgacgcaa atgggcggta
     5401 ggcgtgtacg gtgggaggtc tatataagca gagctcgttt agtgaaccgt cagatctcta
     5461 gaagctgggt accagctgct agcgccacca tgccaatggg ttcactgcaa ccacttgcga
     5521 cgctgtacct cttgggcatg ctggtggcat ccgttctggc agttgagaat ctctgggtca
     5581 ctgtttacta cggcgtgccc gtatggaagg aagccacgac gacattgttc tgtgcgagcg
     5641 atgcgaaggc atacgacact gaggtccaca atgtctgggc aacacacgcg tgcgtcccta
     5701 ccgaccccaa tccccaggaa gtggtccttg aaaatgtcac tgagaatttt aatatgtgga
     5761 agaataatat ggttgagcag atgcatgagg acatcatttc tctgtgggat cagtccctca
     5821 aaccttgtgt caagttgacg gatctgtgtg taaccctcaa ctgcaccgat ttgaggaatg
     5881 taaccaatat caacaattct agtgaaggta tgaggggaga gatcaaaaac tgctccttca
     5941 acataaccac ctccattcga gacaaggtaa aaaaggatta cgctctcttt tatagattgg
     6001 acgtggtacc cattgacaat gataacacca gttataggct gataaactgt aatacctcaa
     6061 cgattacaca agcttgtcct aaagtatcat tcgaacccat tccgatccat tactgtacgc
     6121 cggccggttt cgccatcctt aaatgtaagg ataagaaatt caatggtact ggcccgtgca
     6181 aaaatgtatc tacggtccag tgtacacacg gcataagacc cgtaatgtct acgcaactcc
     6241 tgcttaacgg gagcctggcc gaagaggaag ttgtaatcag aagtagtaac ttcactgata
     6301 atgccaagaa catcattgtg cagttgaaag agtcagtcga gatcaactgt accaggccga
     6361 acaataatac acgaaagtca atacacatag ggcccggaag ggcattctac accactgggg
     6421 atataatcgg agatatacgc caagcgcatt gcaatatcag taggaccaag tggaacaata
     6481 cgctgaacca aatcgctaca aagcttaagg aacaatttgg gaataacaag acaatagtat
     6541 ttaatcaatc atctgggggc gacccggaga tagtcatgca ttcatttaat tgcggcggag
     6601 aatggtttta ttgtaattcc acacagcttt ttaatagcac atggaatttc aacggaacat
     6661 ggaatctgac acagtccaat ggtacggagg ggaatgacac aattaccctt ccctgtcgca
     6721 ttaaacagat aattaatatg tggcaagaag tcggaaaggc gatgtatgct cctcctattc
     6781 gagggcagat aaggtgctca tcaaatatca ccggcctcat actgactcgg gacggcggaa
     6841 ataatcacaa caatgacact gagacatttc gcccaggtgg aggtgatatg cgggataact
     6901 ggcgctccga attgtacaag tacaaagttg tcaagattga gccactcgga gtggccccga
     6961 caaaggctaa gaggcgggtc gtacaaaggg agaaacgcgc ggtaggaacc attggagcca
     7021 tgttcttggg tttcttgggc gcagcgggat ccaccatggg ggcggcctct atcacactga
     7081 ccgtgcaggc aagactcctc ttgagcggca tcgtgcaaca gcaaaacaat ctcttgcgcg
     7141 ctattgaggc tcaacagcac ctgcttcaac ttaccgtatg gggcatcaaa caattgcagg
     7201 ctcgggtctt ggctgtcgag aggtatttgc gggatcagca acttttgggt atgtggggtt
     7261 gttccgggaa gctcatctgc acaacggctg tcccatggaa tgccagttgg tccaacaaga
     7321 ccctggacat gatctggaat aacatgactt ggatggagtg ggagagagaa atcgacaact
     7381 acacgggact catctatacc ttgattgagg agtctcagaa ccaacaagag aagaacgagc
     7441 aggaactgct ggagaatgac aagtgggcca gtctgtggaa ttggttcgat ataaccaact
     7501 ggctttggta catcaaaata tttataatga tcgttggtgg tctgattggt cttaggattg
     7561 ttttcaccgt cctgagcatt gtcaaccgcg tgaggcaggg ttattctccc ctttcctttc
     7621 aaactcactt gcccgcccct agggggccag accgccctga ggggattgaa gaggagggcg
     7681 gcgaccgcga ccgagatagg agtgtgcggc tcgtggatgg gtttctggcc ctcttctggg
     7741 atgaccttcg gtccctctgt cttttctcct atcatcgcct gagggatctt cttttgattg
     7801 tagctaggat cgtcgagctg ttggggcgcc ggggatggga ggcccttaaa tactggtgga
     7861 atttgttgca atattggtcc caagagctca ggaacagtgc ggtgtctctt ttgaacgcga
     7921 cagccatcgc agtagccgag ggaaccgacc gagttataga gatagtccaa cggatctacc
     7981 gagctatcct ccacatccca acgagaattc gacaaggcct tgagcggctc cttctttagt
     8041 aactcgaggc cggcaaggcc ggatccagac atgataagat acattgatga gtttggacaa
     8101 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
     8161 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt
     8221 atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa
     8281 tgtggtatgg ctgattatga tccggctgcc tcgcgcgttt cggtgatgac ggtgaaaacc
     8341 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
     8401 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaggt
     8461 cgactctaga ggatcgatcc ccgccccgga cgaactaaac ctgactacga catctctgcc
     8521 ccttcttcgc ggggcagtgc atgtaatccc ttcagttggt tggtacaact tgccaactgg
     8581 gccctgttcc acatgtgaca cgggggggga ccaaacacaa aggggttctc tgactgtagt
     8641 tgacatcctt ataaatggat gtgcacattt gccaacactg agtggctttc atcctggagc
     8701 agactttgca gtctgtggac tgcaacacaa cattgccttt atgtgtaact cttggctgaa
     8761 gctcttacac caatgctggg ggacatgtac ctcccagggg cccaggaaga ctacgggagg
     8821 ctacaccaac gtcaatcaga ggggcctgtg tagctaccga taagcggacc ctcaagaggg
     8881 cattagcaat agtgtttata aggccccctt gttaacccta aacgggtagc atatgcttcc
     8941 cgggtagtag tatatactat ccagactaac cctaattcaa tagcatatgt tacccaacgg
     9001 gaagcatatg ctatcgaatt agggttagta aaagggtcct aaggaacagc gatatctccc
     9061 accccatgag ctgtcacggt tttatttaca tggggtcagg attccacgag ggtagtgaac
     9121 cattttagtc acaagggcag tggctgaaga tcaaggagcg ggcagtgaac tctcctgaat
     9181 cttcgcctgc ttcttcattc tccttcgttt agctaataga ataactgctg agttgtgaac
     9241 agtaaggtgt atgtgaggtg ctcgaaaaca aggtttcagg tgacgccccc agaataaaat
     9301 ttggacgggg ggttcagtgg tggcattgtg ctatgacacc aatataaccc tcacaaaccc
     9361 cttgggcaat aaatactagt gtaggaatga aacattctga atatctttaa caatagaaat
     9421 ccatggggtg gggacaagcc gtaaagactg gatgtccatc tcacacgaat ttatggctat
     9481 gggcaacaca taatcctagt gcaatatgat actggggtta ttaagatgtg tcccaggcag
     9541 ggaccaagac aggtgaacca tgttgttaca ctctatttgt aacaagggga aagagagtgg
     9601 acgccgacag cagcggactc cactggttgt ctctaacacc cccgaaaatt aaacggggct
     9661 ccacgccaat ggggcccata aacaaagaca agtggccact cttttttttg aaattgtgga
     9721 gtgggggcac gcgtcagccc ccacacgccg ccctgcggtt ttggactgta aaataagggt
     9781 gtaataactt ggctgattgt aaccccgcta accactgcgg tcaaaccact tgcccacaaa
     9841 accactaatg gcaccccggg gaatacctgc ataagtaggt gggcgggcca agataggggc
     9901 gcgattgctg cgatctggag gacaaattac acacacttgc gcctgagcgc caagcacagg
     9961 gttgttggtc ctcatattca cgaggtcgct gagagcacgg tgggctaatg ttgccatggg
    10021 tagcatatac tacccaaata tctggatagc atatgctatc ctaatctata tctgggtagc
    10081 ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt
    10141 atatgctatc ctaatttata tctgggtagc ataggctatc ctaatctata tctgggtagc
    10201 atatgctatc ctaatctata tctgggtagt atatgctatc ctaatctgta tccgggtagc
    10261 atatgctatc ctaatagaga ttagggtagt atatgctatc ctaatttata tctgggtagc
    10321 atatactacc caaatatctg gatagcatat gctatcctaa tctatatctg ggtagcatat
    10381 gctatcctaa tctatatctg ggtagcatag gctatcctaa tctatatctg ggtagcatat
    10441 gctatcctaa tctatatctg ggtagtatat gctatcctaa tttatatctg ggtagcatag
    10501 gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagtatat
    10561 gctatcctaa tctgtatccg ggtagcatat gctatcctca tgcatataca gtcagcatat
    10621 gatacccagt agtagagtgg gagtgctatc ctttgcatat gccgccacct cccaaggggg
    10681 cgtgaatttt cgctgcttgt ccttttcctg ctggttgctc ccattcttag gtgaatttaa
    10741 ggaggccagg ctaaagccgt cgcatgtctg attgctcacc aggtaaatgt cgctaatgtt
    10801 ttccaacgcg agaaggtgtt gagcgcggag ctgagtgacg tgacaacatg ggtatgccca
    10861 attgccccat gttgggagga cgaaaatggt gacaagacag atggccagaa atacaccaac
    10921 agcacgcatg atgtctactg gggatttatt ctttagtgcg ggggaataca cggcttttaa
    10981 tacgattgag ggcgtctcct aacaagttac atcactcctg cccttcctca ccctcatctc
    11041 catcacctcc ttcatctccg tcatctccgt catcaccctc cgcggcagcc ccttccacca
    11101 taggtggaaa ccagggaggc aaatctactc catcgtcaaa gctgcacaca gtcaccctga
    11161 tattgcaggt aggagcgggc tttgtcataa caaggtcctt aatcgcatcc ttcaaaacct
    11221 cagcaaatat atgagtttgt aaaaagacca tgaaataaca gacaatggac tcccttagcg
    11281 ggccaggttg tgggccgggt ccaggggcca ttccaaaggg gagacgactc aatggtgtaa
    11341 gacgacattg tggaatagca agggcagttc ctcgccttag gttgtaaagg gaggtcttac
    11401 tacctccata tacgaacaca ccggcgaccc aagttccttc gtcggtagtc ctttctacgt
    11461 gactcctagc caggagagct cttaaacctt ctgcaatgtt ctcaaatttc gggttggaac
    11521 ctccttgacc acgatgcttt ccaaaccacc ctcctttttt gcgcctgcct ccatcaccct
    11581 gaccccgggg tccagtgctt gggccttctc ctgggtcatc tgcggggccc tgctctatcg
    11641 ctcccggggg cacgtcaggc tcaccatctg ggccaccttc ttggtggtat tcaaaataat
    11701 cggcttcccc tacagggtgg aaaaatggcc ttctacctgg agggggcctg cgcggtggag
    11761 acccggatga tgatga
//
