LOCUS       sequence-240417-       10964 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the Q769.d22 HIV-1 
            isolate; C-terminal truncation; QES mutant with enhanced binding to 
            PG16.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10964)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 10964)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-Q769-QES.i03.c04-754*
FEATURES             Location/Qualifiers
     source          1..10964
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      691..2480
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     3040..3062
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     polyA_signal    3205..3339
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3242..3261)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3296..3315
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     RBS             3955..3963
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     primer_bind     complement(5627..5651)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(5648..5851)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(5677..5697)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5852..6231
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     polyA_signal    6885..6933
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     6958..6977
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(6975..8012)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     promoter        complement(8055..8200)
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     rep_origin      complement(8631..9219)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8711..8730)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9390..10181,10182..10250))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10013..10032
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10251..10355)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10423..10441
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gtaagacctc cctttacaac ctaaggcgag gaactgccct tgctattcca caatgtcgtc
       61 ttacaccatt gagtcgtctc ccctttggaa tggcccctgg acccggccca caacctggcc
      121 cgctaaggga gtccattgtc tgttatttca tggtcttttt acaaactcat atatttgctg
      181 aggttttgaa ggatgcgatt aaggaccttg ttatgacaaa gcccgctcct acctgcaata
      241 tcagggtgac tgtgtgcagc tttgacgatg gagtagattt gcctccctgg tttccaccta
      301 tggtggaagg ggctgccgcg gagggtgatg acggagatga cggagatgaa ggaggtgatg
      361 gagatgaggg tgaggaaggg caggagtgat gtaacttgtt aggagacgcc ctcaatcgta
      421 ttaaaagccg tgtattcccc cgcactaaag aataaatccc cagtagacat catgcgtgct
      481 gttggtgtat ttctggccat ctgtcttgtc accattttcg tcctcccaac atggggcaat
      541 tgggcatacc catgttgtca cgtcactcag ctccgcgctc aacaccttct cgcgttggaa
      601 aacattagcg acatttacct ggtgagcaat cagacatgcg acggctttag cctggcctcc
      661 ttaaattcac ctaagaatgg gagcaaccag caggaaaagg acaagcagcg aaaattcacg
      721 cccccttggg aggtggcggc atatgcaaag gatagcactc ccactctact actgggtatc
      781 atatgctgac tgtatatgca tgaggatagc atatgctacc cggatacaga ttaggatagc
      841 atatactacc cagatataga ttaggatagc atatgctacc cagatataga ttaggatagc
      901 ctatgctacc cagatataaa ttaggatagc atatactacc cagatataga ttaggatagc
      961 atatgctacc cagatataga ttaggatagc ctatgctacc cagatataga ttaggatagc
     1021 atatgctacc cagatataga ttaggatagc atatgctatc cagatatttg ggtagtatat
     1081 gctacccaga tataaattag gatagcatat actaccctaa tctctattag gatagcatat
     1141 gctacccgga tacagattag gatagcatat actacccaga tatagattag gatagcatat
     1201 gctacccaga tatagattag gatagcctat gctacccaga tataaattag gatagcatat
     1261 actacccaga tatagattag gatagcatat gctacccaga tatagattag gatagcctat
     1321 gctacccaga tatagattag gatagcatat gctatccaga tatttgggta gtatatgcta
     1381 cccatggcaa cattagccca ccgtgctctc agcgacctcg tgaatatgag gaccaacaac
     1441 cctgtgcttg gcgctcaggc gcaagtgtgt gtaatttgtc ctccagatcg cagcaatcgc
     1501 gcccctatct tggcccgccc acctacttat gcaggtattc cccggggtgc cattagtggt
     1561 tttgtgggca agtggtttga ccgcagtggt tagcggggtt acaatcagcc aagttattac
     1621 acccttattt tacagtccaa aaccgcaggg cggcgtgtgg gggctgacgc gtgcccccac
     1681 tccacaattt caaaaaaaag agtggccact tgtctttgtt tatgggcccc attggcgtgg
     1741 agccccgttt aattttcggg ggtgttagag acaaccagtg gagtccgctg ctgtcggcgt
     1801 ccactctctt tccccttgtt acaaatagag tgtaacaaca tggttcacct gtcttggtcc
     1861 ctgcctggga cacatcttaa taaccccagt atcatattgc actaggatta tgtgttgccc
     1921 atagccataa attcgtgtga gatggacatc cagtctttac ggcttgtccc caccccatgg
     1981 atttctattg ttaaagatat tcagaatgtt tcattcctac actagtattt attgcccaag
     2041 gggtttgtga gggttatatt ggtgtcatag cacaatgcca ccactgaacc ccccgtccaa
     2101 attttattct gggggcgtca cctgaaacct tgttttcgag cacctcacat acaccttact
     2161 gttcacaact cagcagttat tctattagct aaacgaagga gaatgaagaa gcaggcgaag
     2221 attcaggaga gttcactgcc cgctccttga tcttcagcca ctgcccttgt gactaaaatg
     2281 gttcactacc ctcgtggaat cctgacccca tgtaaataaa accgtgacag ctcatggggt
     2341 gggagatatc gctgttcctt aggacccttt tactaaccct aattcgatag catatgcttc
     2401 ccgttgggta acatatgcta ttgaattagg gttagtctgg atagtatata ctactacccg
     2461 ggaagcatat gctacccgtt tagggttaac aagggggcct tataaacact attgctaatg
     2521 ccctcttgag ggtccgctta tcggtagcta cacaggcccc tctgattgac gttggtgtag
     2581 cctcccgtag tcttcctggg cccctgggag gtacatgtcc cccagcattg gtgtaagagc
     2641 ttcagccaag agttacacat aaaggcaatg ttgtgttgca gtccacagac tgcaaagtct
     2701 gctccaggat gaaagccact cagtgttggc aaatgtgcac atccatttat aaggatgtca
     2761 actacagtca gagaacccct ttgtgtttgg tccccccccg tgtcacatgt ggaacagggc
     2821 ccagttggca agttgtacca accaactgaa gggattacat gcactgcccc gcgaagaagg
     2881 ggcagagatg tcgtagtcag gtttagttcg tccggggcgg ggatcgatcc tctagagtcg
     2941 acctcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     3001 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     3061 ggttttcacc gtcatcaccg aaacgcgcga ggcagccgga tcataatcag ccataccaca
     3121 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
     3181 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
     3241 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     3301 ttgtccaaac tcatcaatgt atcttatcat gtctggatcc ggccttgccg gcctcgagtt
     3361 atcaaagaaa cccagagacc agtcttatgc ttcggtcccg atcctgctca cctccttcct
     3421 cctctatccg gcctggccta tccaaatccc gagggtttgg ggtatgggtt tgaaaggaga
     3481 gcggggaata accttgcctg accctattga tgacagaaag cactgcgaac gcaatccgaa
     3541 gccctatgag cccgccaact atcattatga aaatctttat gtaccacagc cagttactga
     3601 tgtcgaacca gttccacaaa ttagcccatt tgtcgagcgc cagcaagtcc tgttcgtttt
     3661 tctcttgttg cctttgtgac tcctcaataa gatcgtagat aatctgggtg taattgttaa
     3721 tctccttgtc ccactgcatc cacgtcatat tatcccatat ttctgactgt gatttatttg
     3781 accatgagga attccatggg accgtggttg tgcagatgaa ctttccacta catccccaca
     3841 ttcccagcag ttgctgatcc ttaaggtatc tttcaaccgc gagtacccgc gcctgcagtt
     3901 gcttaatgcc ccaaacggta agcttcagca ggtgctgctg agcctctatg gccctaagga
     3961 ggttattctg ctgctgcaca atcccactca aaagctggcg cgcctgaacc gtcagggtga
     4021 tggaggcggc ccccatcgtt gatccggctg cgccaaggaa accgagaaag aacgcgccaa
     4081 atcctaccgc ccgtttttct ctctccacga cccgtctctt ggccttagtt ggagccactc
     4141 caagcggttc tatctttaca actttgtatt tatagagctc ggacctccaa ttatctctca
     4201 tgtccccgcc cccaggtcta aacgtttcct ctgtactgtt gatgatcccc ccatccctcg
     4261 tcaggataag tccggttatg ttactttcgc atctgatctc cccctggata gggggtgcgt
     4321 aaattgcctg gcctactctc tgccacatgt tgatgatctg ctttatccga cattgaagcg
     4381 taattgtatc gttagactcg gtccgattag gttcttgcgt tccgttccat atcccattaa
     4441 agagcccgga agtgttacaa tagaaccact ctcccctaca attaaaacta tgcgtagtta
     4501 tttccaaatc cccaccgaga ctattattaa atatgatcgt acggtttctg aagtgggtcc
     4561 tgagttgcgt tgccacatcc tgaagggtct tattccattg ttggcgatta acattacagt
     4621 gcgctttcct tatatcccct attatgtccg tcgcgtaaaa aactttccca ggcccgagat
     4681 gtatactttt tcgcgtgtta ttacctgggc gcgtgcaatt aatttgcaca gagttgttaa
     4741 actgtattat aatgtttttc gcattatttg taatattttc ggatcgtacc attaccttac
     4801 cctcggcaag gcttccatta aggagcagct gggtactcat gaccggttta atcccatgcg
     4861 tgcattgtac tgttgagacg tttttacaaa gtccagtgcc gttaaagccc ttgtcgttac
     4921 atttcaggat agcatagcct gcaggcgcac aatagtgaat agggatcggc tcgaaagaaa
     4981 cttttgggca agcctgcgtg atctcagacg tgttgcagtt aatgagccga taccttgagt
     5041 gactactgtt ctggttggtt tttgtttcga ggggtacaac gtccagtcga tagaacaaag
     5101 agtaaacgtt ctgcttttta tccttcaatt cggttgtcat gttaaagctg cagttcttaa
     5161 tctcttcttt catatcatcg gtaatgttgc tgacacttgg aatgttatta ctattggaac
     5221 aattcagcgt gacacaaaga ggcgtaagtt tcacacacgg ctgcagtgac tggtcccaca
     5281 aagagatgat gtctgtatgc atctgttcaa ccatgttatt cttccacatg ttgaattcct
     5341 cggttacatt ccccaatggt acttcttggg gggatggatc tgtcggaacg cacgcatgtg
     5401 tagcccaaac attgtgtgct tctcggtcat acgcttttgc atcacttgca caaaagaggg
     5461 ttgtttcagc atcccgccac actggcacgc cgtagtacac ggttacccag agattgcccg
     5521 ccgccagaac gctcgctacc aacataccca gcaaataaag agtagcgagg ggttgcaaag
     5581 accccatggg catggtggcg ctagcagctg gtacccagct tctagagatc tgacggttca
     5641 ctaaacgagc tctgcttata tagacctccc accgtacacg cctaccgccc atttgcgtca
     5701 acggggcggg gttattacga cattttggaa agtcccgttg attttggtgc caaaacaaac
     5761 tcccattgac gtcaatgggg tggagacttg gaaatccccg tgagtcaaac cgctatccac
     5821 gcccattggt gtactgccaa aaccgcatca ccatggtaat agcgatgact aatacgtaga
     5881 tgtactgcca agtaggaaag tcccgtaagg tcatgtactg ggcataatgc caggcgggcc
     5941 atttaccgtc attgacgtca atagggggcg gacttggcat atgatacact tgatgtactg
     6001 ccaagtgggc agtttaccgt aaatactcca cccattgacg tcaatggaaa gtccctattg
     6061 gcgttactat gggaacatac gtcattattg acgtcaatgg gcgggggtcg ttgggcggtc
     6121 agccaggcgg gccatttacc gtaagttatg taacgcggaa ctccatatat gggctatgaa
     6181 ctaatgaccc cgtaattgat tactattaat aactagtcaa taatcaatgt caacatggcg
     6241 gtcatattgg acatgagcca atataaatgt acatattatg atatagatac aacgtatgca
     6301 atggccaata gccaatattg atttatgcta tataaccaat gactaatatg gctaattgcc
     6361 aatattgatt caatgtatag atcttccata cctaccagtt ctgcgcctgc agcaatggca
     6421 acaacgttgc ccggatctgc gatgataagc tgtcaaacat gagaattggt cgactagctt
     6481 ggcacgccag aaatccgcgc ggtggttttt gggggtcggg ggtgtttggc agccacagac
     6541 gcccggtgtt cgtgtcgcgc cagtacatgc ggtccatgcc caggccatcc aaaaaccatg
     6601 ggtctgtctg ctcagtccag tcgtggacct gaccccacgc aacgcccaaa ataataaccc
     6661 ccacgaacca taaaccattc cccatggggg accccgtccc taacccacgg ggccagtggc
     6721 tatggcaggg cctgccgccc cgacgttggc tgcgagccct gggccttcac ccgaacttgg
     6781 ggggtggggt ggggaaaagg aagaaacgcg ggcgtattgg ccccaatggg gtctcggtgg
     6841 ggtatcgaca gagtgccagc cctgggaccg aaccccgcgt ttatgaacaa acgacccaac
     6901 acccgtgcgt tttattctgt ctttttattg ccgtcatagc gcgggttcct tccggtattg
     6961 tctccttccg tgtttcagtt agcctccccc atctcccgat ccggacgagt gctggggcgt
     7021 cggtttccac tatcggcgag tacttctaca cagccatcgg tccagacggc cgcgcttctg
     7081 cgggcgattt gtgtacgccc gacagtcccg gctccggatc ggacgattgc gtcgcatcga
     7141 ccctgcgccc aagctgcatc atcgaaattg ccgtcaacca agctctgata gagttggtca
     7201 agaccaatgc ggagcatata cgcccggagc cgcggcgatc ctgcaagctc cggatgcctc
     7261 cgctcgaagt agcgcgtctg ctgctccata caagccaacc acggcctcca gaagaagatg
     7321 ttggcgacct cgtattggga atccccgaac atcgcctcgc tccagtcaat gaccgctgtt
     7381 atgcggccat tgtccgtcag gacattgttg gagccgaaat ccgcgtgcac gaggtgccgg
     7441 acttcggggc agtcctcggc ccaaagcatc agctcatcga gagcctgcgc gacggacgca
     7501 ctgacggtgt cgtccatcac agtttgccag tgatacacat ggggatcagc aatcgcgcat
     7561 atgaaatcac gccatgtagt gtattgaccg attccttgcg gtccgaatgg gccgaacccg
     7621 ctcgtctggc taagatcggc cgcagcgatc gcatccatgg cctccgcgac cggctgcaga
     7681 acagcgggca gttcggtttc aggcaggtct tgcaacgtga caccctgtgc acggcgggag
     7741 atgcaatagg tcaggctctc gctgaattcc ccaatgtcaa gcacttccgg aatcgggagc
     7801 gcggccgatg caaagtgccg ataaacataa cgatctttgt agaaaccatc ggcgcagcta
     7861 tttacccgca ggacatatcc acgccctcct acatcgaagc tgaaagcacg agattcttcg
     7921 ccctccgaga gctgcatcag gtcggagacg ctgtcgaact tttcgatcag aaacttctcg
     7981 acagacgtcg cggtgagttc aggctttttc atatctcatt gccccccggg atctgcggca
     8041 cgctgttgac gctgttaagc gggtcgctgc agggtcgctc ggtgttcgag gccacacgcg
     8101 tcaccttaat atgcgaagtg gacctcggac cgcgccgccc cgactgcatc tgcgtgttcg
     8161 aattcgccaa tgacaagacg ctgggcgggg tttgtgtcat catagaacta aagacatgca
     8221 aatatatttc ttccggggac accgccagca aacgcgagca acgggccacg gggatgaagc
     8281 agggcggcac ctcgctaacg gattcaccac tccaagaatt ggagccaatc aattcttgcg
     8341 gagaactgtg aatgcgcaaa ccaacccttg gcagaacata tccatcgcgt ccgccatctc
     8401 cagcagccgc acgcggcgca tctcggggcc gacgcgctgg gctacgtctt gctggcgttc
     8461 gcgacgcgag gctggatggc cttccccatt atgattcttc tcgcttccgg cggcatcggg
     8521 atgcccgcgt tgcaggccat gctgtccagg caggtagatg acgaccatca gggacagctt
     8581 caaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     8641 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     8701 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     8761 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     8821 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     8881 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     8941 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     9001 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     9061 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     9121 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     9181 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     9241 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     9301 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     9361 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     9421 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     9481 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     9541 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     9601 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     9661 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctgcagg catcgtggtg
     9721 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     9781 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     9841 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     9901 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     9961 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg ggataatacc
    10021 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
    10081 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
    10141 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
    10201 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
    10261 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
    10321 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
    10381 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
    10441 ccctttcgtc ttcaagaatt ctcatgtttg acagcttatc atcgataagc tgatcctcac
    10501 aggccgcacc cagcttttct tccgttgccc cagtagcatc tctgtctggt gaccttgaag
    10561 aggaagagga ggggtcccga gaatccccat ccctaccgtc cagcaaaaag ggggacgagg
    10621 aatttgaggc ctggcttgag gctcaggacg caaatcttga ggatgttcag cgggagtttt
    10681 ccgggctgcg agtaattggt gatgaggacg aggatggttc ggaggatggg gaattttcag
    10741 acctggatct gtctgacagc gaccatgaag gggatgaggg tgggggggct gttggagggg
    10801 gcaggagtct gcactccctg tattcactga gcgtcgtcta ataaagatgt ctattgatct
    10861 cttttagtgt gaatcatgtc tgacgagggg ccaggtacag gacctggaaa tggcctagga
    10921 gagaagggag acacatctgg accagaaggc tccggcggca gtgg
//
