LOCUS       sequence-240419-       11549 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the 001428 HIV-1 isolate.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11549)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 11549)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-001428gp160
FEATURES             Location/Qualifiers
     source          1..11549
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      1041..2830
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     3390..3412
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     polyA_signal    3555..3689
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3592..3611)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3646..3665
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6328..6352)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(6349..6552)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(6378..6398)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        6553..6932
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     polyA_signal    7586..7634
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     7659..7678
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(7676..8713)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     promoter        complement(8756..8901)
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     rep_origin      complement(9332..9920)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9412..9431)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10091..10882,10883..10951))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10714..10733
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10952..11056)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11124..11142
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 caggccccct ccaggtagaa ggccattttt ccaccctgta ggggaagccg attattttga
       61 ataccaccaa gaaggtggcc cagatggtga gcctgacgtg cccccgggag cgatagagca
      121 gggccccgca gatgacccag gagaaggccc aagcactgga ccccggggtc agggtgatgg
      181 aggcaggcgc aaaaaaggag ggtggtttgg aaagcatcgt ggtcaaggag gttccaaccc
      241 gaaatttgag aacattgcag aaggtttaag agctctcctg gctaggagtc acgtagaaag
      301 gactaccgac gaaggaactt gggtcgccgg tgtgttcgta tatggaggta gtaagacctc
      361 cctttacaac ctaaggcgag gaactgccct tgctattcca caatgtcgtc ttacaccatt
      421 gagtcgtctc ccctttggaa tggcccctgg acccggccca caacctggcc cgctaaggga
      481 gtccattgtc tgttatttca tggtcttttt acaaactcat atatttgctg aggttttgaa
      541 ggatgcgatt aaggaccttg ttatgacaaa gcccgctcct acctgcaata tcagggtgac
      601 tgtgtgcagc tttgacgatg gagtagattt gcctccctgg tttccaccta tggtggaagg
      661 ggctgccgcg gagggtgatg acggagatga cggagatgaa ggaggtgatg gagatgaggg
      721 tgaggaaggg caggagtgat gtaacttgtt aggagacgcc ctcaatcgta ttaaaagccg
      781 tgtattcccc cgcactaaag aataaatccc cagtagacat catgcgtgct gttggtgtat
      841 ttctggccat ctgtcttgtc accattttcg tcctcccaac atggggcaat tgggcatacc
      901 catgttgtca cgtcactcag ctccgcgctc aacaccttct cgcgttggaa aacattagcg
      961 acatttacct ggtgagcaat cagacatgcg acggctttag cctggcctcc ttaaattcac
     1021 ctaagaatgg gagcaaccag caggaaaagg acaagcagcg aaaattcacg cccccttggg
     1081 aggtggcggc atatgcaaag gatagcactc ccactctact actgggtatc atatgctgac
     1141 tgtatatgca tgaggatagc atatgctacc cggatacaga ttaggatagc atatactacc
     1201 cagatataga ttaggatagc atatgctacc cagatataga ttaggatagc ctatgctacc
     1261 cagatataaa ttaggatagc atatactacc cagatataga ttaggatagc atatgctacc
     1321 cagatataga ttaggatagc ctatgctacc cagatataga ttaggatagc atatgctacc
     1381 cagatataga ttaggatagc atatgctatc cagatatttg ggtagtatat gctacccaga
     1441 tataaattag gatagcatat actaccctaa tctctattag gatagcatat gctacccgga
     1501 tacagattag gatagcatat actacccaga tatagattag gatagcatat gctacccaga
     1561 tatagattag gatagcctat gctacccaga tataaattag gatagcatat actacccaga
     1621 tatagattag gatagcatat gctacccaga tatagattag gatagcctat gctacccaga
     1681 tatagattag gatagcatat gctatccaga tatttgggta gtatatgcta cccatggcaa
     1741 cattagccca ccgtgctctc agcgacctcg tgaatatgag gaccaacaac cctgtgcttg
     1801 gcgctcaggc gcaagtgtgt gtaatttgtc ctccagatcg cagcaatcgc gcccctatct
     1861 tggcccgccc acctacttat gcaggtattc cccggggtgc cattagtggt tttgtgggca
     1921 agtggtttga ccgcagtggt tagcggggtt acaatcagcc aagttattac acccttattt
     1981 tacagtccaa aaccgcaggg cggcgtgtgg gggctgacgc gtgcccccac tccacaattt
     2041 caaaaaaaag agtggccact tgtctttgtt tatgggcccc attggcgtgg agccccgttt
     2101 aattttcggg ggtgttagag acaaccagtg gagtccgctg ctgtcggcgt ccactctctt
     2161 tccccttgtt acaaatagag tgtaacaaca tggttcacct gtcttggtcc ctgcctggga
     2221 cacatcttaa taaccccagt atcatattgc actaggatta tgtgttgccc atagccataa
     2281 attcgtgtga gatggacatc cagtctttac ggcttgtccc caccccatgg atttctattg
     2341 ttaaagatat tcagaatgtt tcattcctac actagtattt attgcccaag gggtttgtga
     2401 gggttatatt ggtgtcatag cacaatgcca ccactgaacc ccccgtccaa attttattct
     2461 gggggcgtca cctgaaacct tgttttcgag cacctcacat acaccttact gttcacaact
     2521 cagcagttat tctattagct aaacgaagga gaatgaagaa gcaggcgaag attcaggaga
     2581 gttcactgcc cgctccttga tcttcagcca ctgcccttgt gactaaaatg gttcactacc
     2641 ctcgtggaat cctgacccca tgtaaataaa accgtgacag ctcatggggt gggagatatc
     2701 gctgttcctt aggacccttt tactaaccct aattcgatag catatgcttc ccgttgggta
     2761 acatatgcta ttgaattagg gttagtctgg atagtatata ctactacccg ggaagcatat
     2821 gctacccgtt tagggttaac aagggggcct tataaacact attgctaatg ccctcttgag
     2881 ggtccgctta tcggtagcta cacaggcccc tctgattgac gttggtgtag cctcccgtag
     2941 tcttcctggg cccctgggag gtacatgtcc cccagcattg gtgtaagagc ttcagccaag
     3001 agttacacat aaaggcaatg ttgtgttgca gtccacagac tgcaaagtct gctccaggat
     3061 gaaagccact cagtgttggc aaatgtgcac atccatttat aaggatgtca actacagtca
     3121 gagaacccct ttgtgtttgg tccccccccg tgtcacatgt ggaacagggc ccagttggca
     3181 agttgtacca accaactgaa gggattacat gcactgcccc gcgaagaagg ggcagagatg
     3241 tcgtagtcag gtttagttcg tccggggcgg ggatcgatcc tctagagtcg acctcatggc
     3301 tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     3361 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     3421 gtcatcaccg aaacgcgcga ggcagccgga tcataatcag ccataccaca tttgtagagg
     3481 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
     3541 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     3601 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     3661 tcatcaatgt atcttatcat gtctggatcc ggccttgccg gcctcgagtt actagagcag
     3721 cgcagtctca aacccctgtc gcagccgtct gggtatattg cgtatcgcgc ggcaggttcg
     3781 aagtacgaga tcaatgattc tgtctgtacc ttccgcaaca gctattgcga tcacatcgag
     3841 caaagagatg gcacttttct tcaattcaag cccccagtat tgaacaatgg atccaagata
     3901 cttcaaagct tcccaacccc gctggagccc ccgcagtgag gatcgtccca acaattcgac
     3961 cgcgcgcgct attaccagta tgaaatccct caggcggtga taacaaaaaa gacaaagact
     4021 tcgcaggtca tcccaagcaa gcgccaggaa accgttcaca agcctcacgc tcctatctct
     4081 atcctgctca cctccctctt cttcgattct cccgagtctg tcggggccag tcgggtttgg
     4141 ggtcaatgtt tggaaagaca atggggaata cccctgtctt actcggttga cgatagacaa
     4201 taccgcaaaa atgatgcgaa gaccaatcaa cccacctact atcattatga aaatttttat
     4261 ataccagagc caatttgaga tatcaaacca tgaccaaaga ttcttccagg aatcgagcgc
     4321 gaggaggtct tgctcgttcc tctcttgttg attctgagag tcctccagaa ggcgataaat
     4381 aattccggta tagttgctca cctctctatc ccactgcatc catgtcatat tgtcccagat
     4441 gtccgtgaga cttttgttag accaactaga attccaaggc acggcagtag tacagatcaa
     4501 ttttccactg cacccccaga taccgaggag ttgttggtcc ttcaaatatc tttcgatagc
     4561 aagtacccgg gtttgaagct gtttgatccc ccaaaccgta agctgcaaca agtgttgttg
     4621 agcctcgata gcttgcaaga gatttgattg ctgctgaacg attcctgaca gcaattgtct
     4681 ggcctgcact gtcaaagtga ttgatgcggc tcccatagta gatccagctg cccccaagaa
     4741 accgaggaaa acggcgccga ggccgaccgc tcgtttttcc ctctcaacaa cccgtcgctt
     4801 cgccctcgtc ggggcgacgc ccaggggttt aatctcaacg accttgtact tataaagttc
     4861 gcttctccag ttgtcccgca tatccccccc gccggggcgg aatatctcag tgttgttatt
     4921 ttttcctccg tccctcacga gcaagagccc ggttatatta gaattacaag taatgtttcc
     4981 agctattggt ggagcataca tcgctctccc tacctcttgc cacatattta taatttgctt
     5041 tattctacag ggcagaatta tcgtggaatt gctgttattc gtgccattgg gcatatatgt
     5101 tccgttcggc atatatgtgg agttgaaaag cccagacgta ttgcagtaaa aaaattctcc
     5161 tctgcagtta aaactatgag tcgttatctc caagtcgcca ccactgctag aagtaaattt
     5221 gatggtcttg tttggaaagt gctccgcaag cttttcagac actctcctca gcatttcatg
     5281 ccactttttt tcggagatgt tgcagtgggc ctctcgaatg tttccgatta tgtctccagt
     5341 agcatagaat gtctggcctg gcccgattct gatactcttc cgggtgttgt tattcggtct
     5401 agtacacacg atctccacgc tctgatccag gtggacaatg atggtcttta cattatcggt
     5461 gaggttttcg cttctaatga taatctcctc ttcggccaat gaaccattaa gcagcagttg
     5521 tgtgctaaca acgggcttaa tcccgtgagt gcattgcact gtactaacgt tgttgcaaga
     5581 acctgtgccg ttaaatgtct tattattgca tttgagtatt gcatagcctg ccggggtgca
     5641 gtaatgtatt gggatagggt cgaaatttac ttttgggcac gcctgcgtga tggcagaggt
     5701 gttacaattg atgagaatgt acttagatgc ggaatttgaa tctcctctgt tctctctctc
     5761 caacggaacg aggtccagtc gatagaacag tgcatacgct ttttgtttct tgtctctgat
     5821 ttcggtcgtt gtgttgaaac tgcaattctt catttcatcg ccgttgacct gagtgacgtt
     5881 tacctgagtt gtattcccct gagtcgcgtt gacctgtgtg cattccaagg ttacgcaaag
     5941 tggcgtcagt ttaacacaag gcttgagact ttgtgcccac aaagaaatta cgtcctcgtg
     6001 catctggtct accatatcat tcttccacat gttgaagttt tccgtgacgt ttcccaacac
     6061 catctcttgg gggttagggt ccgtaggtac gcaagcgtgg gttgcccaca cgttatggac
     6121 ttcggtctcg taggccttcg catcagacgc gcaaaacaaa gttgtgcgcg cctccttcca
     6181 cacgggaacc ccatagtaaa cggttaccca gagattctcg actgccagca cacttgctac
     6241 gagcatgccc aggaggtaca acgtggccaa cggttgcagg ctccccatag gcatggtggc
     6301 gctagcagct ggtacccagc ttctagagat ctgacggttc actaaacgag ctctgcttat
     6361 atagacctcc caccgtacac gcctaccgcc catttgcgtc aacggggcgg ggttattacg
     6421 acattttgga aagtcccgtt gattttggtg ccaaaacaaa ctcccattga cgtcaatggg
     6481 gtggagactt ggaaatcccc gtgagtcaaa ccgctatcca cgcccattgg tgtactgcca
     6541 aaaccgcatc accatggtaa tagcgatgac taatacgtag atgtactgcc aagtaggaaa
     6601 gtcccgtaag gtcatgtact gggcataatg ccaggcgggc catttaccgt cattgacgtc
     6661 aatagggggc ggacttggca tatgatacac ttgatgtact gccaagtggg cagtttaccg
     6721 taaatactcc acccattgac gtcaatggaa agtccctatt ggcgttacta tgggaacata
     6781 cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt cagccaggcg ggccatttac
     6841 cgtaagttat gtaacgcgga actccatata tgggctatga actaatgacc ccgtaattga
     6901 ttactattaa taactagtca ataatcaatg tcaacatggc ggtcatattg gacatgagcc
     6961 aatataaatg tacatattat gatatagata caacgtatgc aatggccaat agccaatatt
     7021 gatttatgct atataaccaa tgactaatat ggctaattgc caatattgat tcaatgtata
     7081 gatcttccat acctaccagt tctgcgcctg cagcaatggc aacaacgttg cccggatctg
     7141 cgatgataag ctgtcaaaca tgagaattgg tcgactagct tggcacgcca gaaatccgcg
     7201 cggtggtttt tgggggtcgg gggtgtttgg cagccacaga cgcccggtgt tcgtgtcgcg
     7261 ccagtacatg cggtccatgc ccaggccatc caaaaaccat gggtctgtct gctcagtcca
     7321 gtcgtggacc tgaccccacg caacgcccaa aataataacc cccacgaacc ataaaccatt
     7381 ccccatgggg gaccccgtcc ctaacccacg gggccagtgg ctatggcagg gcctgccgcc
     7441 ccgacgttgg ctgcgagccc tgggccttca cccgaacttg gggggtgggg tggggaaaag
     7501 gaagaaacgc gggcgtattg gccccaatgg ggtctcggtg gggtatcgac agagtgccag
     7561 ccctgggacc gaaccccgcg tttatgaaca aacgacccaa cacccgtgcg ttttattctg
     7621 tctttttatt gccgtcatag cgcgggttcc ttccggtatt gtctccttcc gtgtttcagt
     7681 tagcctcccc catctcccga tccggacgag tgctggggcg tcggtttcca ctatcggcga
     7741 gtacttctac acagccatcg gtccagacgg ccgcgcttct gcgggcgatt tgtgtacgcc
     7801 cgacagtccc ggctccggat cggacgattg cgtcgcatcg accctgcgcc caagctgcat
     7861 catcgaaatt gccgtcaacc aagctctgat agagttggtc aagaccaatg cggagcatat
     7921 acgcccggag ccgcggcgat cctgcaagct ccggatgcct ccgctcgaag tagcgcgtct
     7981 gctgctccat acaagccaac cacggcctcc agaagaagat gttggcgacc tcgtattggg
     8041 aatccccgaa catcgcctcg ctccagtcaa tgaccgctgt tatgcggcca ttgtccgtca
     8101 ggacattgtt ggagccgaaa tccgcgtgca cgaggtgccg gacttcgggg cagtcctcgg
     8161 cccaaagcat cagctcatcg agagcctgcg cgacggacgc actgacggtg tcgtccatca
     8221 cagtttgcca gtgatacaca tggggatcag caatcgcgca tatgaaatca cgccatgtag
     8281 tgtattgacc gattccttgc ggtccgaatg ggccgaaccc gctcgtctgg ctaagatcgg
     8341 ccgcagcgat cgcatccatg gcctccgcga ccggctgcag aacagcgggc agttcggttt
     8401 caggcaggtc ttgcaacgtg acaccctgtg cacggcggga gatgcaatag gtcaggctct
     8461 cgctgaattc cccaatgtca agcacttccg gaatcgggag cgcggccgat gcaaagtgcc
     8521 gataaacata acgatctttg tagaaaccat cggcgcagct atttacccgc aggacatatc
     8581 cacgccctcc tacatcgaag ctgaaagcac gagattcttc gccctccgag agctgcatca
     8641 ggtcggagac gctgtcgaac ttttcgatca gaaacttctc gacagacgtc gcggtgagtt
     8701 caggcttttt catatctcat tgccccccgg gatctgcggc acgctgttga cgctgttaag
     8761 cgggtcgctg cagggtcgct cggtgttcga ggccacacgc gtcaccttaa tatgcgaagt
     8821 ggacctcgga ccgcgccgcc ccgactgcat ctgcgtgttc gaattcgcca atgacaagac
     8881 gctgggcggg gtttgtgtca tcatagaact aaagacatgc aaatatattt cttccgggga
     8941 caccgccagc aaacgcgagc aacgggccac ggggatgaag cagggcggca cctcgctaac
     9001 ggattcacca ctccaagaat tggagccaat caattcttgc ggagaactgt gaatgcgcaa
     9061 accaaccctt ggcagaacat atccatcgcg tccgccatct ccagcagccg cacgcggcgc
     9121 atctcggggc cgacgcgctg ggctacgtct tgctggcgtt cgcgacgcga ggctggatgg
     9181 ccttccccat tatgattctt ctcgcttccg gcggcatcgg gatgcccgcg ttgcaggcca
     9241 tgctgtccag gcaggtagat gacgaccatc agggacagct tcaaggccag caaaaggcca
     9301 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     9361 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     9421 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     9481 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     9541 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     9601 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     9661 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     9721 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     9781 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     9841 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     9901 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     9961 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
    10021 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
    10081 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
    10141 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
    10201 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
    10261 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
    10321 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
    10381 gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg tcgtttggta
    10441 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
    10501 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
    10561 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
    10621 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
    10681 gaccgagttg ctcttgcccg gcgtcaacac gggataatac cgcgccacat agcagaactt
    10741 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
    10801 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
    10861 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
    10921 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
    10981 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
    11041 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
    11101 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt cttcaagaat
    11161 tctcatgttt gacagcttat catcgataag ctgatcctca caggccgcac ccagcttttc
    11221 ttccgttgcc ccagtagcat ctctgtctgg tgaccttgaa gaggaagagg aggggtcccg
    11281 agaatcccca tccctaccgt ccagcaaaaa gggggacgag gaatttgagg cctggcttga
    11341 ggctcaggac gcaaatcttg aggatgttca gcgggagttt tccgggctgc gagtaattgg
    11401 tgatgaggac gaggatggtt cggaggatgg ggaattttca gacctggatc tgtctgacag
    11461 cgaccatgaa ggggatgagg gtgggggggc tgttggaggg ggcaggagtc tgcactccct
    11521 gtattcactg agcgtcgtct aataaagat
//
