LOCUS       sequence-240422-       11059 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the 191084 B7-19 HIV-1 
            isolate.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11059)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 11059)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-191084-gp160
FEATURES             Location/Qualifiers
     source          1..11059
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(454..472)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        540..644
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(645..713,714..1505)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(863..882)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1676..2264
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2165..2184
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2695..2840
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             2883..3920
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(3918..3937)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    3962..4010
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4664..5043
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5044..5247
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5198..5218
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5244..5268
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     polyA_signal    7859..7993
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7883..7902)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7937..7956
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(8136..8158)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8718..10507
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 ctggcccctc gtcagacatg attcacacta aaagagatca atagacatct ttattagacg
       61 acgctcagtg aatacaggga gtgcagactc ctgccccctc caacagcccc cccaccctca
      121 tccccttcat ggtcgctgtc agacagatcc aggtctgaaa attccccatc ctccgaacca
      181 tcctcgtcct catcaccaat tactcgcagc ccggaaaact cccgctgaac atcctcaaga
      241 tttgcgtcct gagcctcaag ccaggcctca aattcctcgt cccccttttt gctggacggt
      301 agggatgggg attctcggga cccctcctct tcctcttcaa ggtcaccaga cagagatgct
      361 actggggcaa cggaagaaaa gctgggtgcg gcctgtgagg atcagcttat cgatgataag
      421 ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg
      481 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
      541 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
      601 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
      661 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
      721 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
      781 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
      841 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc
      901 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
      961 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     1021 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     1081 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     1141 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa
     1201 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     1261 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     1321 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     1381 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     1441 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     1501 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     1561 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     1621 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     1681 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     1741 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     1801 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     1861 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     1921 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     1981 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     2041 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     2101 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     2161 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     2221 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     2281 cctttttacg gttcctggcc ttttgctggc cttgaagctg tccctgatgg tcgtcatcta
     2341 cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa
     2401 tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg
     2461 cgtcggcccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt
     2521 ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct
     2581 tggagtggtg aatccgttag cgaggtgccg ccctgcttca tccccgtggc ccgttgctcg
     2641 cgtttgctgg cggtgtcccc ggaagaaata tatttgcatg tctttagttc tatgatgaca
     2701 caaaccccgc ccagcgtctt gtcattggcg aattcgaaca cgcagatgca gtcggggcgg
     2761 cgcggtccga ggtccacttc gcatattaag gtgacgcgtg tggcctcgaa caccgagcga
     2821 ccctgcagcg acccgcttaa cagcgtcaac agcgtgccgc agatcccggg gggcaatgag
     2881 atatgaaaaa gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg
     2941 acagcgtctc cgacctgatg cagctctcgg agggcgaaga atctcgtgct ttcagcttcg
     3001 atgtaggagg gcgtggatat gtcctgcggg taaatagctg cgccgatggt ttctacaaag
     3061 atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca
     3121 ttggggaatt cagcgagagc ctgacctatt gcatctcccg ccgtgcacag ggtgtcacgt
     3181 tgcaagacct gcctgaaacc gaactgcccg ctgttctgca gccggtcgcg gaggccatgg
     3241 atgcgatcgc tgcggccgat cttagccaga cgagcgggtt cggcccattc ggaccgcaag
     3301 gaatcggtca atacactaca tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt
     3361 atcactggca aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg
     3421 agctgatgct ttgggccgag gactgccccg aagtccggca cctcgtgcac gcggatttcg
     3481 gctccaacaa tgtcctgacg gacaatggcc gcataacagc ggtcattgac tggagcgagg
     3541 cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt cttctggagg ccgtggttgg
     3601 cttgtatgga gcagcagacg cgctacttcg agcggaggca tccggagctt gcaggatcgc
     3661 cgcggctccg ggcgtatatg ctccgcattg gtcttgacca actctatcag agcttggttg
     3721 acggcaattt cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg
     3781 gagccgggac tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc tggaccgatg
     3841 gctgtgtaga agtactcgcc gatagtggaa accgacgccc cagcactcgt ccggatcggg
     3901 agatggggga ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg
     3961 acggcaataa aaagacagaa taaaacgcac gggtgttggg tcgtttgttc ataaacgcgg
     4021 ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata
     4081 cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct
     4141 cgcagccaac gtcggggcgg caggccctgc catagccact ggccccgtgg gttagggacg
     4201 gggtccccca tggggaatgg tttatggttc gtgggggtta ttattttggg cgttgcgtgg
     4261 ggtcaggtcc acgactggac tgagcagaca gacccatggt ttttggatgg cctgggcatg
     4321 gaccgcatgt actggcgcga cacgaacacc gggcgtctgt ggctgccaaa cacccccgac
     4381 ccccaaaaac caccgcgcgg atttctggcg tgccaagcta gtcgaccaat tctcatgttt
     4441 gacagcttat catcgcagat ccgggcaacg ttgttgccat tgctgcaggc gcagaactgg
     4501 taggtatgga agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt
     4561 tatatagcat aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat
     4621 atgtacattt atattggctc atgtccaata tgaccgccat gttgacattg attattgact
     4681 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
     4741 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     4801 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     4861 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     4921 agtccgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     4981 atgaccttac gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     5041 atggtgatgc ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga
     5101 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
     5161 gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta
     5221 cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatctc tagaagctgg
     5281 gtaccagctg ctagcgccac catgccgatg gggtctctcc agccgctggc caccctctac
     5341 ctgttgggta tgttggtggc gtccgtgctg gcgacagaga atctgtgggt tacagtctac
     5401 tacggagttc cagtctggag ggacgcagag acaacacttt tttgtgccag cgacgctaaa
     5461 gcgtatgaca ctgaaatgca taatgtctgg gcaactcatg cctgtgttcc cacagacccg
     5521 aatccacaag aaatagactt ggagaatgta acggagaaat ttaacatgtg gaaaaataac
     5581 atggttgaac aaatgcatac ggatataatc agcctttggg accaatccct gaagccgtgc
     5641 gttaagttga cacccttgtg tgttactctg aactgcacag ccattaccaa tgatacgagg
     5701 gggaatgaga ctggtatcaa tcggaccgtc gagacgaccg aaatgaccaa ctgttcattc
     5761 aatatgacca ctgaacttag ggaccgaaaa aagaaggtca atgcgctttt ttataagctt
     5821 gacatcgttc agataggaga aaattcatct agccagtacc gcctcattaa ttgtaatacc
     5881 agtgttatta cacaagcctg cccaaaagtt accttcgagc caattccaat tcattattgc
     5941 gcgcctgctg gatttgccat ccttaagtgc aaggacaaag agttcaacgg gacaggaact
     6001 tgtaggaacg tgtccagtgt gcaatgcact cacggtataa aacccgtcgt gagcacgcaa
     6061 cttttgctca acgggtccct ggcggaagga caggtgaaga tccggtcaga gaacatctca
     6121 gacaatgcaa aaaccatcat cgtccagttg aatgagtctg ttccaatcaa ttgtactaga
     6181 ccaaacaaca atacacgaag ggggatccac ctgggtccgg gccaaacatt ctttgctacc
     6241 gacataattg gtgacattcg ccaagctcac tgtaatgtta gcgaatcaaa atggaacaag
     6301 gcgctccagg aagttgtgaa acagctgagg cagcactgga acaaaacgat aatcttcaaa
     6361 tcctcatccg gtggggatct cgaaatcacg actcactcct tcaactgtgg aggtgagttc
     6421 ttctactgta acacctcagg cctgttcaat agcacatgga acatagccgg caacagaacg
     6481 aatgatacga agtcaaatga aacaatcacc ctcccgtgtc gaataaaaca gatcgtaaat
     6541 gtttggcaaa gggtaggaca agcgatctac gcgcccccaa ttgcaggagt cattcggtgc
     6601 aattcaaaca tcacaggcct cctcttggta agagatgggg gagctactaa taatacagac
     6661 gaaacgttcc gccctggagg cgggaacatg cgcgataatt ggagaagtga gttgtataaa
     6721 tataaggtgg tcaagataga gcctctgggc gttgcaccga ccagggcgcg caggcgcgtg
     6781 gtggaaaggg agaagagggc tgtaggactc ggagccgtat ttattggatt tcttggggca
     6841 gcgggttcta ccatgggtgc agcttccgtg accttgaccg tacaagccag gcaacttctt
     6901 tctggaatcg ttcaacaaca aagcaatctt ctccgagcaa tcgaggccca gcagcatctc
     6961 ttgaaactta cagtctgggg cataaagcag ctccaggcgc gcgtgctcgc agttgagaga
     7021 tatttgaaag accaacagct gcttggtatt tggggctgtt ctggaaaatt gatatgcccg
     7081 actaccgtac catggaacag tagctggtca aacaaatcac aaaacgaaat ctgggataat
     7141 atgacctggc ttcaatggga caaggagatt tcaaattaca cacagcttat atattcattg
     7201 attgaggaaa gccaaaatca gcaggagaag aacgagcagg agctgttggc gctggacaag
     7261 tgggcatctt tgtggaattg gttcgacatt tccaagtggc tttggtatat cagaatattt
     7321 ataatgatcg tgggcgggtt gataggactt agaattgtat tcacagttct cagcatcatt
     7381 aatagggttc gccagggtta ctccccgctc tcttttcaga cacatccgcc atctccaaca
     7441 ggtcctgata ggcctgggcg gattgaagaa gagggtggtg agcaaggacg ggatagaagc
     7501 accaggctgg tatctggatt cttggcattg gcctgggatg atctcagatc cctcttcctc
     7561 tttagttatc atcacctccg agatctcatc ctcatcgtcg caagggcggt cgagcttctg
     7621 ggccataata gtcttaaggg acttaaactt ggctggaacc ttctcctgta ctggtctaga
     7681 gagttgaaga tttccgcgtt gaatttgttg gataccattg ccatcgcagt ggcgggttgg
     7741 accgaccgag ttatcgagat agggcagaga atttgccggg caatacggaa tattccaaga
     7801 cgaattagac agggcgcgga aagagcattt ctgtagtaac tcgaggccgg caaggccgga
     7861 tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
     7921 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
     7981 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt
     8041 gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatcc
     8101 ggctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
     8161 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
     8221 gcgggtgttg gcgggtgtcg gggcgcagcc atgaggtcga ctctagagga tcgatccccg
     8281 ccccggacga actaaacctg actacgacat ctctgcccct tcttcgcggg gcagtgcatg
     8341 taatcccttc agttggttgg tacaacttgc caactgggcc ctgttccaca tgtgacacgg
     8401 ggggggacca aacacaaagg ggttctctga ctgtagttga catccttata aatggatgtg
     8461 cacatttgcc aacactgagt ggctttcatc ctggagcaga ctttgcagtc tgtggactgc
     8521 aacacaacat tgcctttatg tgtaactctt ggctgaagct cttacaccaa tgctggggga
     8581 catgtacctc ccaggggccc aggaagacta cgggaggcta caccaacgtc aatcagaggg
     8641 gcctgtgtag ctaccgataa gcggaccctc aagagggcat tagcaatagt gtttataagg
     8701 cccccttgtt aaccctaaac gggtagcata tgcttcccgg gtagtagtat atactatcca
     8761 gactaaccct aattcaatag catatgttac ccaacgggaa gcatatgcta tcgaattagg
     8821 gttagtaaaa gggtcctaag gaacagcgat atctcccacc ccatgagctg tcacggtttt
     8881 atttacatgg ggtcaggatt ccacgagggt agtgaaccat tttagtcaca agggcagtgg
     8941 ctgaagatca aggagcgggc agtgaactct cctgaatctt cgcctgcttc ttcattctcc
     9001 ttcgtttagc taatagaata actgctgagt tgtgaacagt aaggtgtatg tgaggtgctc
     9061 gaaaacaagg tttcaggtga cgcccccaga ataaaatttg gacggggggt tcagtggtgg
     9121 cattgtgcta tgacaccaat ataaccctca caaacccctt gggcaataaa tactagtgta
     9181 ggaatgaaac attctgaata tctttaacaa tagaaatcca tggggtgggg acaagccgta
     9241 aagactggat gtccatctca cacgaattta tggctatggg caacacataa tcctagtgca
     9301 atatgatact ggggttatta agatgtgtcc caggcaggga ccaagacagg tgaaccatgt
     9361 tgttacactc tatttgtaac aaggggaaag agagtggacg ccgacagcag cggactccac
     9421 tggttgtctc taacaccccc gaaaattaaa cggggctcca cgccaatggg gcccataaac
     9481 aaagacaagt ggccactctt ttttttgaaa ttgtggagtg ggggcacgcg tcagccccca
     9541 cacgccgccc tgcggttttg gactgtaaaa taagggtgta ataacttggc tgattgtaac
     9601 cccgctaacc actgcggtca aaccacttgc ccacaaaacc actaatggca ccccggggaa
     9661 tacctgcata agtaggtggg cgggccaaga taggggcgcg attgctgcga tctggaggac
     9721 aaattacaca cacttgcgcc tgagcgccaa gcacagggtt gttggtcctc atattcacga
     9781 ggtcgctgag agcacggtgg gctaatgttg ccatgggtag catatactac ccaaatatct
     9841 ggatagcata tgctatccta atctatatct gggtagcata ggctatccta atctatatct
     9901 gggtagcata tgctatccta atctatatct gggtagtata tgctatccta atttatatct
     9961 gggtagcata ggctatccta atctatatct gggtagcata tgctatccta atctatatct
    10021 gggtagtata tgctatccta atctgtatcc gggtagcata tgctatccta atagagatta
    10081 gggtagtata tgctatccta atttatatct gggtagcata tactacccaa atatctggat
    10141 agcatatgct atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt
    10201 agcataggct atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt
    10261 agtatatgct atcctaattt atatctgggt agcataggct atcctaatct atatctgggt
    10321 agcatatgct atcctaatct atatctgggt agtatatgct atcctaatct gtatccgggt
    10381 agcatatgct atcctcatgc atatacagtc agcatatgat acccagtagt agagtgggag
    10441 tgctatcctt tgcatatgcc gccacctccc aagggggcgt gaattttcgc tgcttgtcct
    10501 tttcctgctg gttgctccca ttcttaggtg aatttaagga ggccaggcta aagccgtcgc
    10561 atgtctgatt gctcaccagg taaatgtcgc taatgttttc caacgcgaga aggtgttgag
    10621 cgcggagctg agtgacgtga caacatgggt atgcccaatt gccccatgtt gggaggacga
    10681 aaatggtgac aagacagatg gccagaaata caccaacagc acgcatgatg tctactgggg
    10741 atttattctt tagtgcgggg gaatacacgg cttttaatac gattgagggc gtctcctaac
    10801 aagttacatc actcctgccc ttcctcaccc tcatctccat cacctccttc atctccgtca
    10861 tctccgtcat caccctccgc ggcagcccct tccaccatag gtggaaacca gggaggcaaa
    10921 tctactccat cgtcaaagct gcacacagtc accctgatat tgcaggtagg agcgggcttt
    10981 gtcataacaa ggtccttaat cgcatccttc aaaacctcag caaatatatg agtttgtaaa
    11041 aagaccatga aataacaga
//
