LOCUS       sequence-240428-       11060 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the AD8 HIV-1 isolate; 
            C-terminal truncation; QES mutant for enhanced presentation of 
            quaternary epitopes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11060)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 11060)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-AD8-QES.i08.c04-754*
FEATURES             Location/Qualifiers
     source          1..11060
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(474..492)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        560..664
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(665..733,734..1525)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(883..902)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1696..2284
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2185..2204
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2715..2860
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             2903..3940
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(3938..3957)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    3982..4030
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4684..5063
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5064..5267
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5218..5238
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5264..5288
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     polyA_signal    7585..7719
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7609..7628)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7663..7682
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(7862..7884)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8444..10233
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 gccatttcca ggtcctgtac ctggcccctc gtcagacatg attcacacta aaagagatca
       61 atagacatct ttattagacg acgctcagtg aatacaggga gtgcagactc ctgccccctc
      121 caacagcccc cccaccctca tccccttcat ggtcgctgtc agacagatcc aggtctgaaa
      181 attccccatc ctccgaacca tcctcgtcct catcaccaat tactcgcagc ccggaaaact
      241 cccgctgaac atcctcaaga tttgcgtcct gagcctcaag ccaggcctca aattcctcgt
      301 cccccttttt gctggacggt agggatgggg attctcggga cccctcctct tcctcttcaa
      361 ggtcaccaga cagagatgct actggggcaa cggaagaaaa gctgggtgcg gcctgtgagg
      421 atcagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt
      481 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
      541 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
      601 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
      661 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
      721 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
      781 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
      841 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
      901 atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
      961 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     1021 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     1081 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     1141 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     1201 gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     1261 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     1321 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     1381 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     1441 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     1501 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     1561 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     1621 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     1681 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     1741 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     1801 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     1861 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     1921 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     1981 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     2041 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     2101 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     2161 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     2221 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     2281 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttgaagctg
     2341 tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat
     2401 gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc
     2461 cagcaagacg tagcccagcg cgtcggcccc gagatgcgcc gcgtgcggct gctggagatg
     2521 gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag
     2581 aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccctgcttca
     2641 tccccgtggc ccgttgctcg cgtttgctgg cggtgtcccc ggaagaaata tatttgcatg
     2701 tctttagttc tatgatgaca caaaccccgc ccagcgtctt gtcattggcg aattcgaaca
     2761 cgcagatgca gtcggggcgg cgcggtccga ggtccacttc gcatattaag gtgacgcgtg
     2821 tggcctcgaa caccgagcga ccctgcagcg acccgcttaa cagcgtcaac agcgtgccgc
     2881 agatcccggg gggcaatgag atatgaaaaa gcctgaactc accgcgacgt ctgtcgagaa
     2941 gtttctgatc gaaaagttcg acagcgtctc cgacctgatg cagctctcgg agggcgaaga
     3001 atctcgtgct ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg taaatagctg
     3061 cgccgatggt ttctacaaag atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc
     3121 gattccggaa gtgcttgaca ttggggaatt cagcgagagc ctgacctatt gcatctcccg
     3181 ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg ctgttctgca
     3241 gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat cttagccaga cgagcgggtt
     3301 cggcccattc ggaccgcaag gaatcggtca atacactaca tggcgtgatt tcatatgcgc
     3361 gattgctgat ccccatgtgt atcactggca aactgtgatg gacgacaccg tcagtgcgtc
     3421 cgtcgcgcag gctctcgatg agctgatgct ttgggccgag gactgccccg aagtccggca
     3481 cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg gacaatggcc gcataacagc
     3541 ggtcattgac tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt
     3601 cttctggagg ccgtggttgg cttgtatgga gcagcagacg cgctacttcg agcggaggca
     3661 tccggagctt gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg gtcttgacca
     3721 actctatcag agcttggttg acggcaattt cgatgatgca gcttgggcgc agggtcgatg
     3781 cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt acacaaatcg cccgcagaag
     3841 cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc gatagtggaa accgacgccc
     3901 cagcactcgt ccggatcggg agatggggga ggctaactga aacacggaag gagacaatac
     3961 cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac gggtgttggg
     4021 tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg
     4081 agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt
     4141 tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc catagccact
     4201 ggccccgtgg gttagggacg gggtccccca tggggaatgg tttatggttc gtgggggtta
     4261 ttattttggg cgttgcgtgg ggtcaggtcc acgactggac tgagcagaca gacccatggt
     4321 ttttggatgg cctgggcatg gaccgcatgt actggcgcga cacgaacacc gggcgtctgt
     4381 ggctgccaaa cacccccgac ccccaaaaac caccgcgcgg atttctggcg tgccaagcta
     4441 gtcgaccaat tctcatgttt gacagcttat catcgcagat ccgggcaacg ttgttgccat
     4501 tgctgcaggc gcagaactgg taggtatgga agatctatac attgaatcaa tattggcaat
     4561 tagccatatt agtcattggt tatatagcat aaatcaatat tggctattgg ccattgcata
     4621 cgttgtatct atatcataat atgtacattt atattggctc atgtccaata tgaccgccat
     4681 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
     4741 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     4801 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     4861 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     4921 atcaagtgta tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg
     4981 cctggcatta tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg
     5041 tattagtcat cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat
     5101 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
     5161 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc
     5221 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc
     5281 gtcagatctc tagaagctgg gtaccagctg ctagcgccac catgccaatg ggttcactgc
     5341 aaccacttgc gacgctgtac ctcttgggca tgctggtggc atccgttctg gcagttgaga
     5401 atctctgggt cactgtttac tacggcgtgc ccgtatggaa ggaagccacg acgacattgt
     5461 tctgtgcgag cgatgcgaag gcatacgaca ctgaggtcca caatgtctgg gcaacacacg
     5521 cgtgcgtccc taccgacccc aatccccagg aagtggtcct tgaaaatgtc actgagaatt
     5581 ttaatatgtg gaagaataat atggttgagc agatgcatga ggacatcatt tctctgtggg
     5641 atcagtccct caaaccttgt gtcaagttga cggatctgtg tgtaaccctc aactgcaccg
     5701 atttgaggaa tgtaaccaat atcaacaatt ctagtgaagg tatgagggga gagatcaaaa
     5761 actgctcctt caacataacc acctccattc gagacaaggt aaaaaaggat tacgctctct
     5821 tttatagatt ggacgtggta cccattgaca atgataacac cagttatagg ctgataaact
     5881 gtaatacctc aacgattaca caagcttgtc ctaaagtatc attcgaaccc attccgatcc
     5941 attactgtac gccggccggt ttcgccatcc ttaaatgtaa ggataagaaa ttcaatggta
     6001 ctggcccgtg caaaaatgta tctacggtcc agtgtacaca cggcataaga cccgtaatgt
     6061 ctacgcaact cctgcttaac gggagcctgg ccgaagagga agttgtaatc agaagtagta
     6121 acttcactga taatgccaag aacatcattg tgcagttgaa agagtcagtc gagatcaact
     6181 gtaccaggcc gaacaataat acacgaaagt caatacacat agggcccgga agggcattct
     6241 acaccactgg ggatataatc ggagatatac gccaagcgca ttgcaatatc agtaggacca
     6301 agtggaacaa tacgctgaac caaatcgcta caaagcttaa ggaacaattt gggaataaca
     6361 agacaatagt atttaatcaa tcatctgggg gcgacccgga gatagtcatg cattcattta
     6421 attgcggcgg agaatggttt tattgtaatt ccacacagct ttttaatagc acatggaatt
     6481 tcaacggaac atggaatctg acacagtcca atggtacgga ggggaatgac acaattaccc
     6541 ttccctgtcg cattaaacag ataattaata tgtggcaaga agtcggaaag gcgatgtatg
     6601 ctcctcctat tcgagggcag ataaggtgct catcaaatat caccggcctc atactgactc
     6661 gggacggcgg aaataatcac aacaatgaca ctgagacatt tcgcccaggt ggaggtgata
     6721 tgcgggataa ctggcgctcc gaattgtaca agtacaaagt tgtcaagatt gagccactcg
     6781 gagtggcccc gacaaaggct aagaggcggg tcgtacaaag ggagaaacgc gcggtaggaa
     6841 ccattggagc catgttcttg ggtttcttgg gcgcagcggg atccaccatg ggggcggcct
     6901 ctatcacact gaccgtgcag gcaagactcc tcttgagcgg catcgtgcaa cagcaaaaca
     6961 atctcttgcg cgctattgag gctcaacagc acctgcttca acttaccgta tggggcatca
     7021 aacaattgca ggctcgggtc ttggctgtcg agaggtattt gcgggatcag caacttttgg
     7081 gtatgtgggg ttgttccggg aagctcatct gcacaacggc tgtcccatgg aatgccagtt
     7141 ggtccaacaa gaccctggac atgatctgga ataacatgac ttggatggag tgggagagag
     7201 aaatcgacaa ctacacggga ctcatctata ccttgattga ggagtctcag aaccaacaag
     7261 agaagaacga gcaggaactg ctggagaatg acaagtgggc cagtctgtgg aattggttcg
     7321 atataaccaa ctggctttgg tacatcaaaa tatttataat gatcgttggt ggtctgattg
     7381 gtcttaggat tgttttcacc gtcctgagca ttgtcaaccg cgtgaggcag ggttattctc
     7441 ccctttcctt tcaaactcac ttgcccgccc ctagggggcc agaccgccct gaggggattg
     7501 aagaggaggg cggcgaccgc gaccgagata ggagtgtgcg gctcgtggat gggtttctgt
     7561 gataactcga ggccggcaag gccggatcca gacatgataa gatacattga tgagtttgga
     7621 caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt
     7681 gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat
     7741 tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac
     7801 aaatgtggta tggctgatta tgatccggct gcctcgcgcg tttcggtgat gacggtgaaa
     7861 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     7921 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga
     7981 ggtcgactct agaggatcga tccccgcccc ggacgaacta aacctgacta cgacatctct
     8041 gccccttctt cgcggggcag tgcatgtaat cccttcagtt ggttggtaca acttgccaac
     8101 tgggccctgt tccacatgtg acacgggggg ggaccaaaca caaaggggtt ctctgactgt
     8161 agttgacatc cttataaatg gatgtgcaca tttgccaaca ctgagtggct ttcatcctgg
     8221 agcagacttt gcagtctgtg gactgcaaca caacattgcc tttatgtgta actcttggct
     8281 gaagctctta caccaatgct gggggacatg tacctcccag gggcccagga agactacggg
     8341 aggctacacc aacgtcaatc agaggggcct gtgtagctac cgataagcgg accctcaaga
     8401 gggcattagc aatagtgttt ataaggcccc cttgttaacc ctaaacgggt agcatatgct
     8461 tcccgggtag tagtatatac tatccagact aaccctaatt caatagcata tgttacccaa
     8521 cgggaagcat atgctatcga attagggtta gtaaaagggt cctaaggaac agcgatatct
     8581 cccaccccat gagctgtcac ggttttattt acatggggtc aggattccac gagggtagtg
     8641 aaccatttta gtcacaaggg cagtggctga agatcaagga gcgggcagtg aactctcctg
     8701 aatcttcgcc tgcttcttca ttctccttcg tttagctaat agaataactg ctgagttgtg
     8761 aacagtaagg tgtatgtgag gtgctcgaaa acaaggtttc aggtgacgcc cccagaataa
     8821 aatttggacg gggggttcag tggtggcatt gtgctatgac accaatataa ccctcacaaa
     8881 ccccttgggc aataaatact agtgtaggaa tgaaacattc tgaatatctt taacaataga
     8941 aatccatggg gtggggacaa gccgtaaaga ctggatgtcc atctcacacg aatttatggc
     9001 tatgggcaac acataatcct agtgcaatat gatactgggg ttattaagat gtgtcccagg
     9061 cagggaccaa gacaggtgaa ccatgttgtt acactctatt tgtaacaagg ggaaagagag
     9121 tggacgccga cagcagcgga ctccactggt tgtctctaac acccccgaaa attaaacggg
     9181 gctccacgcc aatggggccc ataaacaaag acaagtggcc actctttttt ttgaaattgt
     9241 ggagtggggg cacgcgtcag cccccacacg ccgccctgcg gttttggact gtaaaataag
     9301 ggtgtaataa cttggctgat tgtaaccccg ctaaccactg cggtcaaacc acttgcccac
     9361 aaaaccacta atggcacccc ggggaatacc tgcataagta ggtgggcggg ccaagatagg
     9421 ggcgcgattg ctgcgatctg gaggacaaat tacacacact tgcgcctgag cgccaagcac
     9481 agggttgttg gtcctcatat tcacgaggtc gctgagagca cggtgggcta atgttgccat
     9541 gggtagcata tactacccaa atatctggat agcatatgct atcctaatct atatctgggt
     9601 agcataggct atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt
     9661 agtatatgct atcctaattt atatctgggt agcataggct atcctaatct atatctgggt
     9721 agcatatgct atcctaatct atatctgggt agtatatgct atcctaatct gtatccgggt
     9781 agcatatgct atcctaatag agattagggt agtatatgct atcctaattt atatctgggt
     9841 agcatatact acccaaatat ctggatagca tatgctatcc taatctatat ctgggtagca
     9901 tatgctatcc taatctatat ctgggtagca taggctatcc taatctatat ctgggtagca
     9961 tatgctatcc taatctatat ctgggtagta tatgctatcc taatttatat ctgggtagca
    10021 taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta
    10081 tatgctatcc taatctgtat ccgggtagca tatgctatcc tcatgcatat acagtcagca
    10141 tatgataccc agtagtagag tgggagtgct atcctttgca tatgccgcca cctcccaagg
    10201 gggcgtgaat tttcgctgct tgtccttttc ctgctggttg ctcccattct taggtgaatt
    10261 taaggaggcc aggctaaagc cgtcgcatgt ctgattgctc accaggtaaa tgtcgctaat
    10321 gttttccaac gcgagaaggt gttgagcgcg gagctgagtg acgtgacaac atgggtatgc
    10381 ccaattgccc catgttggga ggacgaaaat ggtgacaaga cagatggcca gaaatacacc
    10441 aacagcacgc atgatgtcta ctggggattt attctttagt gcgggggaat acacggcttt
    10501 taatacgatt gagggcgtct cctaacaagt tacatcactc ctgcccttcc tcaccctcat
    10561 ctccatcacc tccttcatct ccgtcatctc cgtcatcacc ctccgcggca gccccttcca
    10621 ccataggtgg aaaccaggga ggcaaatcta ctccatcgtc aaagctgcac acagtcaccc
    10681 tgatattgca ggtaggagcg ggctttgtca taacaaggtc cttaatcgca tccttcaaaa
    10741 cctcagcaaa tatatgagtt tgtaaaaaga ccatgaaata acagacaatg gactccctta
    10801 gcgggccagg ttgtgggccg ggtccagggg ccattccaaa ggggagacga ctcaatggtg
    10861 taagacgaca ttgtggaata gcaagggcag ttcctcgcct taggttgtaa agggaggtct
    10921 tactacctcc atatacgaac acaccggcga cccaagttcc ttcgtcggta gtcctttcta
    10981 cgtgactcct agccaggaga gctcttaaac cttctgcaat gttctcaaat ttcgggttgg
    11041 aacctccttg accacgatgc
//
