LOCUS       sequence-240429-       11602 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the B41 HIV-1 isolate.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11602)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 11602)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-B41gp160
FEATURES             Location/Qualifiers
     source          1..11602
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(615..633)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        701..805
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(806..874,875..1666)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1024..1043)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1837..2425
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2326..2345
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2856..3001
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3044..4081
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4079..4098)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4123..4171
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4825..5204
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5205..5408
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5359..5379
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5405..5429
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             7042..7053
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     polyA_signal    8068..8202
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8092..8111)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8146..8165
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(8345..8367)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8927..10716
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 tgagccgccc ggggctcctg gtcttccgcc tcctcgtcct cgtcctcttc cccgtcctcg
       61 tccatggtta tcaccccctc ttctttgagg tccactgccg ccggagcctt ctggtccaga
      121 tgtgtctccc ttctctccta ggccatttcc aggtcctgta cctggcccct cgtcagacat
      181 gattcacact aaaagagatc aatagacatc tttattagac gacgctcagt gaatacaggg
      241 agtgcagact cctgccccct ccaacagccc ccccaccctc atccccttca tggtcgctgt
      301 cagacagatc caggtctgaa aattccccat cctccgaacc atcctcgtcc tcatcaccaa
      361 ttactcgcag cccggaaaac tcccgctgaa catcctcaag atttgcgtcc tgagcctcaa
      421 gccaggcctc aaattcctcg tccccctttt tgctggacgg tagggatggg gattctcggg
      481 acccctcctc ttcctcttca aggtcaccag acagagatgc tactggggca acggaagaaa
      541 agctgggtgc ggcctgtgag gatcagctta tcgatgataa gctgtcaaac atgagaattc
      601 ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
      661 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
      721 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
      781 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
      841 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
      901 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
      961 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     1021 tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg
     1081 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     1141 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     1201 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     1261 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     1321 aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt
     1381 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     1441 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     1501 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     1561 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     1621 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     1681 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     1741 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     1801 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     1861 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     1921 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     1981 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     2041 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     2101 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     2161 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     2221 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     2281 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     2341 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     2401 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     2461 cttttgctgg ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc
     2521 ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat
     2581 ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccc cgagatgcgc
     2641 cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc
     2701 gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta
     2761 gcgaggtgcc gccctgcttc atccccgtgg cccgttgctc gcgtttgctg gcggtgtccc
     2821 cggaagaaat atatttgcat gtctttagtt ctatgatgac acaaaccccg cccagcgtct
     2881 tgtcattggc gaattcgaac acgcagatgc agtcggggcg gcgcggtccg aggtccactt
     2941 cgcatattaa ggtgacgcgt gtggcctcga acaccgagcg accctgcagc gacccgctta
     3001 acagcgtcaa cagcgtgccg cagatcccgg ggggcaatga gatatgaaaa agcctgaact
     3061 caccgcgacg tctgtcgaga agtttctgat cgaaaagttc gacagcgtct ccgacctgat
     3121 gcagctctcg gagggcgaag aatctcgtgc tttcagcttc gatgtaggag ggcgtggata
     3181 tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa gatcgttatg tttatcggca
     3241 ctttgcatcg gccgcgctcc cgattccgga agtgcttgac attggggaat tcagcgagag
     3301 cctgacctat tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac
     3361 cgaactgccc gctgttctgc agccggtcgc ggaggccatg gatgcgatcg ctgcggccga
     3421 tcttagccag acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc aatacactac
     3481 atggcgtgat ttcatatgcg cgattgctga tccccatgtg tatcactggc aaactgtgat
     3541 ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc tttgggccga
     3601 ggactgcccc gaagtccggc acctcgtgca cgcggatttc ggctccaaca atgtcctgac
     3661 ggacaatggc cgcataacag cggtcattga ctggagcgag gcgatgttcg gggattccca
     3721 atacgaggtc gccaacatct tcttctggag gccgtggttg gcttgtatgg agcagcagac
     3781 gcgctacttc gagcggaggc atccggagct tgcaggatcg ccgcggctcc gggcgtatat
     3841 gctccgcatt ggtcttgacc aactctatca gagcttggtt gacggcaatt tcgatgatgc
     3901 agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg
     3961 tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc
     4021 cgatagtgga aaccgacgcc ccagcactcg tccggatcgg gagatggggg aggctaactg
     4081 aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga
     4141 ataaaacgca cgggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg
     4201 cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt
     4261 tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg
     4321 gcaggccctg ccatagccac tggccccgtg ggttagggac ggggtccccc atggggaatg
     4381 gtttatggtt cgtgggggtt attattttgg gcgttgcgtg gggtcaggtc cacgactgga
     4441 ctgagcagac agacccatgg tttttggatg gcctgggcat ggaccgcatg tactggcgcg
     4501 acacgaacac cgggcgtctg tggctgccaa acacccccga cccccaaaaa ccaccgcgcg
     4561 gatttctggc gtgccaagct agtcgaccaa ttctcatgtt tgacagctta tcatcgcaga
     4621 tccgggcaac gttgttgcca ttgctgcagg cgcagaactg gtaggtatgg aagatctata
     4681 cattgaatca atattggcaa ttagccatat tagtcattgg ttatatagca taaatcaata
     4741 ttggctattg gccattgcat acgttgtatc tatatcataa tatgtacatt tatattggct
     4801 catgtccaat atgaccgcca tgttgacatt gattattgac tagttattaa tagtaatcaa
     4861 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     4921 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     4981 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     5041 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtccgccc cctattgacg
     5101 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta cgggactttc
     5161 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     5221 agtacaccaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     5281 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     5341 ataaccccgc cccgttgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     5401 gcagagctcg tttagtgaac cgtcagatct ctagaagctg ggtaccagct gctagcgcca
     5461 ccatgccaat gggtagtctt cagcctctcg ccaccttgta tctgctcggt atgctggttg
     5521 ccagcgttct ggctaacttg tgggtgaccg tatactacgg ggtcccggta tggaaggagg
     5581 caaacactac actcttctgt gcttccgacg caaaggggta tgatacggag aagcataatg
     5641 tctgggcgac acatgcatgt gtgcctacag acccaaaccc gcaggaagtc ctccttggta
     5701 ataacgtgac cgagaacttt aatatgtgga aaaataacat ggtagagcag atgcatgaag
     5761 acattataag cttgtgggat caatctctta agccgtgtgt gaagctgaca ccgctttgcg
     5821 taacactcaa ttgtacggat ttgacgaaaa atagcactac caataactcc accgctacta
     5881 acaataccac gaacaatagt accgaaaaaa tggagatgga caaaggcgag atgaagaatt
     5941 gctcctttaa tatcacaaca aatattcgag ataagatgca gaaagaatac gctttgctgt
     6001 acaagcttga catcgtgccg atcgacaatg gcaagaataa cagcaacacc aattatcgcc
     6061 tgatttcatg taatacctca gtaattacgc aatcatgtcc caaagtgtca tttcagccca
     6121 ttcctattca ttattgtgca cccgccggtt ttgcaatact taagtgtaat aataagacct
     6181 ttaatggaag cgggccttgt actaatgtgt cttccgtgca atgcactcac ggtatccgac
     6241 ctgttgtttc aacccagctt ttgctgaacg gaagtctcgc tgaagaagaa atcgtgatta
     6301 ggtccgctaa cttctctgac aacacgaaga ctataattgt ccaacttaag gaagcggtcg
     6361 aaataaactg tacgcgcccg aataataaca cgcgaaaatc aattaacatc gggcctgggc
     6421 gagcttttta tgctactggc aacataatag gggacatccg ccaagctcat tgtaacatat
     6481 cacggacaaa gtggaataat acgttgacag aaattgctaa gaagctgaac aagcagtacg
     6541 agaatagaac aatagcattt aatcaatcct ccggaggcga tcttgagatc gtcatgcatt
     6601 cattcaactg cggtggagaa tttttctatt gcaacacttc ccagctgttc agagatacgt
     6661 ggaacaacac ttggaacggc atatggaaca acgatacgga ggtcaacgag actatcacac
     6721 ttccgtgtag aatcaaacaa ataatcaata tgtggcaaga agtcgggaag gcgatgtatg
     6781 ccccgccaat ccgcggccaa atcaattgct catcaaatat tacgggactc ctgctcaccc
     6841 gcgacggggg agaaagcaac aagaccatag aggtcttccg gcccggtggc ggagacatga
     6901 gggacaactg gaggtccgag ctgtataagt acaaagttgt taagatagag cccctcgggg
     6961 ttgctcccac caaagctaaa agaagagtgg tgcaacgaga gaaaagagcc gtcggcttgg
     7021 gtgccctctt cgtcggattt ttgggagctg caggtagcac aatgggtgct gcctctataa
     7081 cgctgaccgt acaagctcga cagcttctta gtggtatcgt tcaacaacaa aacaatctct
     7141 tgcgagcaat agaagcccaa cagcatttgc ttcaactgac agtatggggc gttaagcagt
     7201 tgcaagcgcg ggtcttggcg gtcgaacgat acctgaaaga ccagcaactc ctgggcttgt
     7261 ggggatgcag cggcaaactt atatgtacca cagctgtacc ttggaacacc agctggagta
     7321 acaagagtta cgattatatc tgggacaaca tgacgtggat gcaatgggag cgcgagattg
     7381 ataactatac aggagttatc tacaagctga ttgaggaatc ccagaaccaa caggagaaaa
     7441 atgaacaaga acttttggcc ctggataaat gggcaaatct gtggaactgg tttgacatca
     7501 caagatggct ttggtacatt aagattttta ttatgatcgt agggggtttg gtagggcttc
     7561 ggatcgtttt tgctgtgctc tccatcgtga accgagtcag gcaggggtac tcacctttga
     7621 gcctgcaaac gcactttcct gcgccaagag gaccggatcg accagaggga acggaggagg
     7681 aaggtggtga ccgggatcgg gatcgcagca ctcccctcgt ggatgggttc cttgcaataa
     7741 tttgggtaga ccttaggagt ttgtttcttt tctcctacca ccgcctgaga gacctcctcc
     7801 tgatagtgac acggattgtc gaactgcttg gtcgcagagg ttgggaggcc cttaagtact
     7861 ggtggaatct tcttcagtat tggtcccaag agcttaggaa ctctgcggtc tccttgttca
     7921 atgccacagc gatcgcagtg gctgagggaa ccgaccgagt cattgaggcg gtccaaagaa
     7981 ttggccgggc aattcttcac atacctcgaa gaataaggca gggctttgag agagcactgc
     8041 agtagtaact cgaggccggc aaggccggat ccagacatga taagatacat tgatgagttt
     8101 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
     8161 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt
     8221 cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc
     8281 tacaaatgtg gtatggctga ttatgatccg gctgcctcgc gcgtttcggt gatgacggtg
     8341 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     8401 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca
     8461 tgaggtcgac tctagaggat cgatccccgc cccggacgaa ctaaacctga ctacgacatc
     8521 tctgcccctt cttcgcgggg cagtgcatgt aatcccttca gttggttggt acaacttgcc
     8581 aactgggccc tgttccacat gtgacacggg gggggaccaa acacaaaggg gttctctgac
     8641 tgtagttgac atccttataa atggatgtgc acatttgcca acactgagtg gctttcatcc
     8701 tggagcagac tttgcagtct gtggactgca acacaacatt gcctttatgt gtaactcttg
     8761 gctgaagctc ttacaccaat gctgggggac atgtacctcc caggggccca ggaagactac
     8821 gggaggctac accaacgtca atcagagggg cctgtgtagc taccgataag cggaccctca
     8881 agagggcatt agcaatagtg tttataaggc ccccttgtta accctaaacg ggtagcatat
     8941 gcttcccggg tagtagtata tactatccag actaacccta attcaatagc atatgttacc
     9001 caacgggaag catatgctat cgaattaggg ttagtaaaag ggtcctaagg aacagcgata
     9061 tctcccaccc catgagctgt cacggtttta tttacatggg gtcaggattc cacgagggta
     9121 gtgaaccatt ttagtcacaa gggcagtggc tgaagatcaa ggagcgggca gtgaactctc
     9181 ctgaatcttc gcctgcttct tcattctcct tcgtttagct aatagaataa ctgctgagtt
     9241 gtgaacagta aggtgtatgt gaggtgctcg aaaacaaggt ttcaggtgac gcccccagaa
     9301 taaaatttgg acggggggtt cagtggtggc attgtgctat gacaccaata taaccctcac
     9361 aaaccccttg ggcaataaat actagtgtag gaatgaaaca ttctgaatat ctttaacaat
     9421 agaaatccat ggggtgggga caagccgtaa agactggatg tccatctcac acgaatttat
     9481 ggctatgggc aacacataat cctagtgcaa tatgatactg gggttattaa gatgtgtccc
     9541 aggcagggac caagacaggt gaaccatgtt gttacactct atttgtaaca aggggaaaga
     9601 gagtggacgc cgacagcagc ggactccact ggttgtctct aacacccccg aaaattaaac
     9661 ggggctccac gccaatgggg cccataaaca aagacaagtg gccactcttt tttttgaaat
     9721 tgtggagtgg gggcacgcgt cagcccccac acgccgccct gcggttttgg actgtaaaat
     9781 aagggtgtaa taacttggct gattgtaacc ccgctaacca ctgcggtcaa accacttgcc
     9841 cacaaaacca ctaatggcac cccggggaat acctgcataa gtaggtgggc gggccaagat
     9901 aggggcgcga ttgctgcgat ctggaggaca aattacacac acttgcgcct gagcgccaag
     9961 cacagggttg ttggtcctca tattcacgag gtcgctgaga gcacggtggg ctaatgttgc
    10021 catgggtagc atatactacc caaatatctg gatagcatat gctatcctaa tctatatctg
    10081 ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg
    10141 ggtagtatat gctatcctaa tttatatctg ggtagcatag gctatcctaa tctatatctg
    10201 ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa tctgtatccg
    10261 ggtagcatat gctatcctaa tagagattag ggtagtatat gctatcctaa tttatatctg
    10321 ggtagcatat actacccaaa tatctggata gcatatgcta tcctaatcta tatctgggta
    10381 gcatatgcta tcctaatcta tatctgggta gcataggcta tcctaatcta tatctgggta
    10441 gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaattta tatctgggta
    10501 gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
    10561 gtatatgcta tcctaatctg tatccgggta gcatatgcta tcctcatgca tatacagtca
    10621 gcatatgata cccagtagta gagtgggagt gctatccttt gcatatgccg ccacctccca
    10681 agggggcgtg aattttcgct gcttgtcctt ttcctgctgg ttgctcccat tcttaggtga
    10741 atttaaggag gccaggctaa agccgtcgca tgtctgattg ctcaccaggt aaatgtcgct
    10801 aatgttttcc aacgcgagaa ggtgttgagc gcggagctga gtgacgtgac aacatgggta
    10861 tgcccaattg ccccatgttg ggaggacgaa aatggtgaca agacagatgg ccagaaatac
    10921 accaacagca cgcatgatgt ctactgggga tttattcttt agtgcggggg aatacacggc
    10981 ttttaatacg attgagggcg tctcctaaca agttacatca ctcctgccct tcctcaccct
    11041 catctccatc acctccttca tctccgtcat ctccgtcatc accctccgcg gcagcccctt
    11101 ccaccatagg tggaaaccag ggaggcaaat ctactccatc gtcaaagctg cacacagtca
    11161 ccctgatatt gcaggtagga gcgggctttg tcataacaag gtccttaatc gcatccttca
    11221 aaacctcagc aaatatatga gtttgtaaaa agaccatgaa ataacagaca atggactccc
    11281 ttagcgggcc aggttgtggg ccgggtccag gggccattcc aaaggggaga cgactcaatg
    11341 gtgtaagacg acattgtgga atagcaaggg cagttcctcg ccttaggttg taaagggagg
    11401 tcttactacc tccatatacg aacacaccgg cgacccaagt tccttcgtcg gtagtccttt
    11461 ctacgtgact cctagccagg agagctctta aaccttctgc aatgttctca aatttcgggt
    11521 tggaacctcc ttgaccacga tgctttccaa accaccctcc ttttttgcgc ctgcctccat
    11581 caccctgacc ccggggtcca gt
//
