LOCUS       sequence-240434-       11022 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for soluble gp140 from the DU422 HIV-1 
            isolate with a transmembrane tether.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11022)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 11022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-DU422gp140-TM
FEATURES             Location/Qualifiers
     source          1..11022
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(426..444)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        512..616
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(617..685,686..1477)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(835..854)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1648..2236
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2137..2156
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2667..2812
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             2855..3892
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(3890..3909)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    3934..3982
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4636..5015
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5016..5219
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5170..5190
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5216..5240
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             7284..7301
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    7444..7578
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7468..7487)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7522..7541
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(7721..7743)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8303..10092
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 taaaagagat caatagacat ctttattaga cgacgctcag tgaatacagg gagtgcagac
       61 tcctgccccc tccaacagcc cccccaccct catccccttc atggtcgctg tcagacagat
      121 ccaggtctga aaattcccca tcctccgaac catcctcgtc ctcatcacca attactcgca
      181 gcccggaaaa ctcccgctga acatcctcaa gatttgcgtc ctgagcctca agccaggcct
      241 caaattcctc gtcccccttt ttgctggacg gtagggatgg ggattctcgg gacccctcct
      301 cttcctcttc aaggtcacca gacagagatg ctactggggc aacggaagaa aagctgggtg
      361 cggcctgtga ggatcagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg
      421 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
      481 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
      541 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
      601 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
      661 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
      721 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
      781 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
      841 tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
      901 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
      961 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     1021 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     1081 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     1141 gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga
     1201 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     1261 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     1321 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     1381 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     1441 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     1501 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     1561 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     1621 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     1681 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     1741 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     1801 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     1861 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     1921 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     1981 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     2041 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     2101 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     2161 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     2221 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     2281 gccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc
     2341 gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg
     2401 cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc ccgagatgcg ccgcgtgcgg
     2461 ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca
     2521 gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc
     2581 cgccctgctt catccccgtg gcccgttgct cgcgtttgct ggcggtgtcc ccggaagaaa
     2641 tatatttgca tgtctttagt tctatgatga cacaaacccc gcccagcgtc ttgtcattgg
     2701 cgaattcgaa cacgcagatg cagtcggggc ggcgcggtcc gaggtccact tcgcatatta
     2761 aggtgacgcg tgtggcctcg aacaccgagc gaccctgcag cgacccgctt aacagcgtca
     2821 acagcgtgcc gcagatcccg gggggcaatg agatatgaaa aagcctgaac tcaccgcgac
     2881 gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc
     2941 ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg
     3001 ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc
     3061 ggccgcgctc ccgattccgg aagtgcttga cattggggaa ttcagcgaga gcctgaccta
     3121 ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc
     3181 cgctgttctg cagccggtcg cggaggccat ggatgcgatc gctgcggccg atcttagcca
     3241 gacgagcggg ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga
     3301 tttcatatgc gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac
     3361 cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc
     3421 cgaagtccgg cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg
     3481 ccgcataaca gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt
     3541 cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt
     3601 cgagcggagg catccggagc ttgcaggatc gccgcggctc cgggcgtata tgctccgcat
     3661 tggtcttgac caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc
     3721 gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat
     3781 cgcccgcaga agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg
     3841 aaaccgacgc cccagcactc gtccggatcg ggagatgggg gaggctaact gaaacacgga
     3901 aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc
     3961 acgggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc
     4021 gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt ttccccaccc
     4081 caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc ggcaggccct
     4141 gccatagcca ctggccccgt gggttaggga cggggtcccc catggggaat ggtttatggt
     4201 tcgtgggggt tattattttg ggcgttgcgt ggggtcaggt ccacgactgg actgagcaga
     4261 cagacccatg gtttttggat ggcctgggca tggaccgcat gtactggcgc gacacgaaca
     4321 ccgggcgtct gtggctgcca aacacccccg acccccaaaa accaccgcgc ggatttctgg
     4381 cgtgccaagc tagtcgacca attctcatgt ttgacagctt atcatcgcag atccgggcaa
     4441 cgttgttgcc attgctgcag gcgcagaact ggtaggtatg gaagatctat acattgaatc
     4501 aatattggca attagccata ttagtcattg gttatatagc ataaatcaat attggctatt
     4561 ggccattgca tacgttgtat ctatatcata atatgtacat ttatattggc tcatgtccaa
     4621 tatgaccgcc atgttgacat tgattattga ctagttatta atagtaatca attacggggt
     4681 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
     4741 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
     4801 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
     4861 acttggcagt acatcaagtg tatcatatgc caagtccgcc ccctattgac gtcaatgacg
     4921 gtaaatggcc cgcctggcat tatgcccagt acatgacctt acgggacttt cctacttggc
     4981 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacacca
     5041 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
     5101 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aataaccccg
     5161 ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc
     5221 gtttagtgaa ccgtcagatc tctagaagct gggtaccagc tgctagcgcc accatgccca
     5281 tgggttccct ccaaccactt gcaacgcttt atctgctcgg catgcttgta gcgtcagtgc
     5341 ttgcaaatct cgacctttgg gtcaccgtat actacggagt ccccgtgtgg aaagaggcta
     5401 agacgaccct gttttgtgcg tcagacgcga aagcgtatga caaggaagtg cataacgtgt
     5461 gggcaacaca cgcatgtgtt ccgacggacc ctaacccgca ggaaatcgtc cttgaaaacg
     5521 tgaccgaaaa cttcaacatg tggaagaacg acatggttga ccaaatgcac gaggacataa
     5581 tcagtctttg ggatcaatcc ttgaagccct gtgtgaagct gacgcctttg tgcgtaactt
     5641 tgaactgtaa aaacgtaaat atttcagcga atgctaacgc gaccgctaca cttaattcaa
     5701 gtatgaacgg cgaaataaag aattgctcct tcaacaccac gacagaactt agagataaaa
     5761 agcaaaaagt ctacgccctt ttctataaac cggatgtcgt gccacttaac ggtggtgagc
     5821 acaatgagac aggtgagtac attctcatca actgcaattc atctacaata acgcaagcat
     5881 gtccgaaagt ttccttcgat ccgatcccta ttcactactg cgctcctgca gggtacgcca
     5941 tactgaagtg taacaataaa acgtttaacg gaacaggccc ttgcaataat gtaagtactg
     6001 tccagtgcac gcatggcata aaacccgtcg tgagcacaca acttcttctt aacggatcac
     6061 tcgccgagga agagatcatc gtgcgaagtg agaacttgac gaataatata aagacgatca
     6121 tagtccatct caacaagtcc gtcgagatta aatgcactag gcccaacaac aatacgcgga
     6181 aatccgtacg gataggtcct ggacaaacat tctatgctac cggtgagatt ataggtgata
     6241 tacgcgaagc tcattgcaat atcagtaggg agacctggaa ttccactttg atccaagtga
     6301 aagaaaaact gcgggagcat tacaacaaaa cgatcaaatt tgaaccttct tctggaggtg
     6361 acctggaggt tacgacgcac agttttaact gtaggggaga attcttctac tgcgatacga
     6421 ccaagctgtt caacgagact aagctgttta acgagtcaga atacgttgac aataagacta
     6481 ttatcttgcc ctgccggata aagcaaatta tcaatatgtg gcaagaagtg ggaagggcaa
     6541 tgtacgcgcc gccgattgaa gggaatatta cttgcaagag caacataact ggtcttcttc
     6601 tcacatggga tggtggagag aacagtaccg aaggcgtttt taggcctggc ggtggcaata
     6661 tgaaggataa ctggcgctca gaactctata agtacaaagt cgtcgaaata aagcccttgg
     6721 gcgttgcgcc tacgaaaagt aaacgaaaag tagtagggag ggaaaagcga gcggttggct
     6781 tgggtgcagt gcttctcggg ttcctgggag ctgcggggtc aacaatggga gctgcttcca
     6841 taaccttgac tgtgcaggca aggcagctgc tgagtgggat agtgcagcaa caatccaatc
     6901 tgcttagggc aattgaagct cagcaacatc ttttgcagct cacagtctgg ggcataaagc
     6961 aactgcaaac cagagtcctc gcgattgagc ggtatcttaa ggaccagcaa ctgctgggac
     7021 tctgggggtg ttccgggaag cttatttgtg ccactgcggt accatggaac tcctcttggt
     7081 ccaacaagtc tctgggcgat atttgggaca atatgacgtg gatgcagtgg gatcgcgaga
     7141 tatccaacta caccaacacg atcttccggc ttctcgaaga cagtcagaat caacaagaga
     7201 agaatgagaa ggatctgttg gcccttgaca gctggaaaaa tctttggaat tggtttgaca
     7261 ttactaactc cggaggcagc ggacatcatc accatcacca cggctctgga gccggaagtg
     7321 ctatccccat catgggtatc gttgctggcc tggttgtcct tgcagctgta gtcactggag
     7381 ctgcggtcgc tgctgtgctg tggagaaaga agagctcaga ttgactcgag gccggcaagg
     7441 ccggatccag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
     7501 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
     7561 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
     7621 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat
     7681 gatccggctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
     7741 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
     7801 cgtcagcggg tgttggcggg tgtcggggcg cagccatgag gtcgactcta gaggatcgat
     7861 ccccgccccg gacgaactaa acctgactac gacatctctg ccccttcttc gcggggcagt
     7921 gcatgtaatc ccttcagttg gttggtacaa cttgccaact gggccctgtt ccacatgtga
     7981 cacggggggg gaccaaacac aaaggggttc tctgactgta gttgacatcc ttataaatgg
     8041 atgtgcacat ttgccaacac tgagtggctt tcatcctgga gcagactttg cagtctgtgg
     8101 actgcaacac aacattgcct ttatgtgtaa ctcttggctg aagctcttac accaatgctg
     8161 ggggacatgt acctcccagg ggcccaggaa gactacggga ggctacacca acgtcaatca
     8221 gaggggcctg tgtagctacc gataagcgga ccctcaagag ggcattagca atagtgttta
     8281 taaggccccc ttgttaaccc taaacgggta gcatatgctt cccgggtagt agtatatact
     8341 atccagacta accctaattc aatagcatat gttacccaac gggaagcata tgctatcgaa
     8401 ttagggttag taaaagggtc ctaaggaaca gcgatatctc ccaccccatg agctgtcacg
     8461 gttttattta catggggtca ggattccacg agggtagtga accattttag tcacaagggc
     8521 agtggctgaa gatcaaggag cgggcagtga actctcctga atcttcgcct gcttcttcat
     8581 tctccttcgt ttagctaata gaataactgc tgagttgtga acagtaaggt gtatgtgagg
     8641 tgctcgaaaa caaggtttca ggtgacgccc ccagaataaa atttggacgg ggggttcagt
     8701 ggtggcattg tgctatgaca ccaatataac cctcacaaac cccttgggca ataaatacta
     8761 gtgtaggaat gaaacattct gaatatcttt aacaatagaa atccatgggg tggggacaag
     8821 ccgtaaagac tggatgtcca tctcacacga atttatggct atgggcaaca cataatccta
     8881 gtgcaatatg atactggggt tattaagatg tgtcccaggc agggaccaag acaggtgaac
     8941 catgttgtta cactctattt gtaacaaggg gaaagagagt ggacgccgac agcagcggac
     9001 tccactggtt gtctctaaca cccccgaaaa ttaaacgggg ctccacgcca atggggccca
     9061 taaacaaaga caagtggcca ctcttttttt tgaaattgtg gagtgggggc acgcgtcagc
     9121 ccccacacgc cgccctgcgg ttttggactg taaaataagg gtgtaataac ttggctgatt
     9181 gtaaccccgc taaccactgc ggtcaaacca cttgcccaca aaaccactaa tggcaccccg
     9241 gggaatacct gcataagtag gtgggcgggc caagataggg gcgcgattgc tgcgatctgg
     9301 aggacaaatt acacacactt gcgcctgagc gccaagcaca gggttgttgg tcctcatatt
     9361 cacgaggtcg ctgagagcac ggtgggctaa tgttgccatg ggtagcatat actacccaaa
     9421 tatctggata gcatatgcta tcctaatcta tatctgggta gcataggcta tcctaatcta
     9481 tatctgggta gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaattta
     9541 tatctgggta gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta
     9601 tatctgggta gtatatgcta tcctaatctg tatccgggta gcatatgcta tcctaataga
     9661 gattagggta gtatatgcta tcctaattta tatctgggta gcatatacta cccaaatatc
     9721 tggatagcat atgctatcct aatctatatc tgggtagcat atgctatcct aatctatatc
     9781 tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc
     9841 tgggtagtat atgctatcct aatttatatc tgggtagcat aggctatcct aatctatatc
     9901 tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct aatctgtatc
     9961 cgggtagcat atgctatcct catgcatata cagtcagcat atgataccca gtagtagagt
    10021 gggagtgcta tcctttgcat atgccgccac ctcccaaggg ggcgtgaatt ttcgctgctt
    10081 gtccttttcc tgctggttgc tcccattctt aggtgaattt aaggaggcca ggctaaagcc
    10141 gtcgcatgtc tgattgctca ccaggtaaat gtcgctaatg ttttccaacg cgagaaggtg
    10201 ttgagcgcgg agctgagtga cgtgacaaca tgggtatgcc caattgcccc atgttgggag
    10261 gacgaaaatg gtgacaagac agatggccag aaatacacca acagcacgca tgatgtctac
    10321 tggggattta ttctttagtg cgggggaata cacggctttt aatacgattg agggcgtctc
    10381 ctaacaagtt acatcactcc tgcccttcct caccctcatc tccatcacct ccttcatctc
    10441 cgtcatctcc gtcatcaccc tccgcggcag ccccttccac cataggtgga aaccagggag
    10501 gcaaatctac tccatcgtca aagctgcaca cagtcaccct gatattgcag gtaggagcgg
    10561 gctttgtcat aacaaggtcc ttaatcgcat ccttcaaaac ctcagcaaat atatgagttt
    10621 gtaaaaagac catgaaataa cagacaatgg actcccttag cgggccaggt tgtgggccgg
    10681 gtccaggggc cattccaaag gggagacgac tcaatggtgt aagacgacat tgtggaatag
    10741 caagggcagt tcctcgcctt aggttgtaaa gggaggtctt actacctcca tatacgaaca
    10801 caccggcgac ccaagttcct tcgtcggtag tcctttctac gtgactccta gccaggagag
    10861 ctcttaaacc ttctgcaatg ttctcaaatt tcgggttgga acctccttga ccacgatgct
    10921 ttccaaacca ccctcctttt ttgcgcctgc ctccatcacc ctgaccccgg ggtccagtgc
    10981 ttgggccttc tcctgggtca tctgcggggc cctgctctat cg
//
