LOCUS       sequence-240435-       11093 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the 191084 B7-19 HIV-1 
            isolate; QES mutant with enhanced binding to PG16.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11093)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 11093)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-191084-QES.i06.c07
FEATURES             Location/Qualifiers
     source          1..11093
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(502..520)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        588..692
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(693..761,762..1553)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(911..930)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1724..2312
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2213..2232
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2743..2888
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             2931..3968
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(3966..3985)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4010..4058
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4712..5091
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5092..5295
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5246..5266
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5292..5316
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     polyA_signal    7616..7750
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7640..7659)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7694..7713
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(7893..7915)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8475..10264
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 gtccagatgt gtctcccttc tctcctaggc catttccagg tcctgtacct ggcccctcgt
       61 cagacatgat tcacactaaa agagatcaat agacatcttt attagacgac gctcagtgaa
      121 tacagggagt gcagactcct gccccctcca acagcccccc caccctcatc cccttcatgg
      181 tcgctgtcag acagatccag gtctgaaaat tccccatcct ccgaaccatc ctcgtcctca
      241 tcaccaatta ctcgcagccc ggaaaactcc cgctgaacat cctcaagatt tgcgtcctga
      301 gcctcaagcc aggcctcaaa ttcctcgtcc ccctttttgc tggacggtag ggatggggat
      361 tctcgggacc cctcctcttc ctcttcaagg tcaccagaca gagatgctac tggggcaacg
      421 gaagaaaagc tgggtgcggc ctgtgaggat cagcttatcg atgataagct gtcaaacatg
      481 agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
      541 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
      601 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
      661 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
      721 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
      781 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
      841 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
      901 ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg
      961 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     1021 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     1081 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     1141 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     1201 ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca
     1261 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     1321 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     1381 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     1441 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     1501 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     1561 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     1621 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     1681 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     1741 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     1801 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     1861 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     1921 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     1981 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     2041 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     2101 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     2161 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     2221 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     2281 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     2341 tcctggcctt ttgctggcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca
     2401 gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga
     2461 aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccccga
     2521 gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt
     2581 ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa
     2641 tccgttagcg aggtgccgcc ctgcttcatc cccgtggccc gttgctcgcg tttgctggcg
     2701 gtgtccccgg aagaaatata tttgcatgtc tttagttcta tgatgacaca aaccccgccc
     2761 agcgtcttgt cattggcgaa ttcgaacacg cagatgcagt cggggcggcg cggtccgagg
     2821 tccacttcgc atattaaggt gacgcgtgtg gcctcgaaca ccgagcgacc ctgcagcgac
     2881 ccgcttaaca gcgtcaacag cgtgccgcag atcccggggg gcaatgagat atgaaaaagc
     2941 ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtctccg
     3001 acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc
     3061 gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat cgttatgttt
     3121 atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt ggggaattca
     3181 gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg caagacctgc
     3241 ctgaaaccga actgcccgct gttctgcagc cggtcgcgga ggccatggat gcgatcgctg
     3301 cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga atcggtcaat
     3361 acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa
     3421 ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt
     3481 gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg
     3541 tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg atgttcgggg
     3601 attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct tgtatggagc
     3661 agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgccg cggctccggg
     3721 cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac ggcaatttcg
     3781 atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg
     3841 tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag
     3901 tactcgccga tagtggaaac cgacgcccca gcactcgtcc ggatcgggag atgggggagg
     3961 ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa
     4021 agacagaata aaacgcacgg gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca
     4081 gggctggcac tctgtcgata ccccaccgag accccattgg ggccaatacg cccgcgtttc
     4141 ttccttttcc ccaccccacc ccccaagttc gggtgaaggc ccagggctcg cagccaacgt
     4201 cggggcggca ggccctgcca tagccactgg ccccgtgggt tagggacggg gtcccccatg
     4261 gggaatggtt tatggttcgt gggggttatt attttgggcg ttgcgtgggg tcaggtccac
     4321 gactggactg agcagacaga cccatggttt ttggatggcc tgggcatgga ccgcatgtac
     4381 tggcgcgaca cgaacaccgg gcgtctgtgg ctgccaaaca cccccgaccc ccaaaaacca
     4441 ccgcgcggat ttctggcgtg ccaagctagt cgaccaattc tcatgtttga cagcttatca
     4501 tcgcagatcc gggcaacgtt gttgccattg ctgcaggcgc agaactggta ggtatggaag
     4561 atctatacat tgaatcaata ttggcaatta gccatattag tcattggtta tatagcataa
     4621 atcaatattg gctattggcc attgcatacg ttgtatctat atcataatat gtacatttat
     4681 attggctcat gtccaatatg accgccatgt tgacattgat tattgactag ttattaatag
     4741 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
     4801 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
     4861 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
     4921 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tccgccccct
     4981 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttacgg
     5041 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg
     5101 ttttggcagt acaccaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
     5161 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
     5221 tgtcgtaata accccgcccc gttgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
     5281 tatataagca gagctcgttt agtgaaccgt cagatctcta gaagctgggt accagctgct
     5341 agcgccacca tgcccatggg tagtctgcag cctttggcta ctctttactt gttgggtatg
     5401 ttggtagcga gcgtcctcgc agtcgggaat ttgtgggtta cagtttatta cggggtcccg
     5461 gtgtggaagg aggcgacgac cacattgttt tgcgcctccg atgcaaaggc ttacgacacg
     5521 gaagttcaca acgtttgggc cacccatgcg tgcgtgccta cggaccctaa tccacaggag
     5581 atgttcatgc ccaatgtcac cgaaaatttt aatatgtgga aaaacgacat ggtaaatcaa
     5641 atgcatgagg atgtcataag tctctgggac caaagtctga agccatgcgt gaaattgacc
     5701 ccgctctgcg tgacactgga gtgtaaaaac gtgtcacgaa ataattccag ttcaaatggt
     5761 actggtacaa acaatgaagc gtatcgagag ggaatgaagg aaataaagaa ctgctctttt
     5821 aatgccactg ccgagcttag agaccggaag cagaacgtgt atgcactgtt ttacaggctt
     5881 gacatcgtac ctcttgcgga gaacagtagc gaatataggc ttatcaattg caacaccagc
     5941 gcaattactc aagcctgccc caaaatgact tttgacccga ttcctataca ctactgcaca
     6001 ccagcagggt acgcaattct taagtgtagc gacaaaacct tcaacggcac tggcccctgt
     6061 tcaaatgtct ccacagttca atgtactcac ggaatacgac cggtcatgag tacacagttg
     6121 ctgctgaacg gttctctggc cgaagaggag atcatcatcc gctctgagaa cctcacgaac
     6181 aatgccaagc caataatcgt acagcttaac cagagcgtgg aaatagtttg cacacgccct
     6241 aacaataaca ctagaaagtc aatcaggatc ggaccagggc agacgtttta tgcgacagga
     6301 ggaataatag gcgacatccg acaagcgtac tgtaacattt cccggaataa atggaacgag
     6361 acattgagaa tggtaggtaa aaaattggcg gaacattttc ctaacaaaac catcaatttt
     6421 acatcctcct ccgggggaga tctggaaatc acgacgcaca gttttacgtg taggggagaa
     6481 ttcttctatt gcaacacctc cggactgttt aatggtacgt acatgcccaa cggtacgtat
     6541 atgttcaact ctacgaatgc cacgatcaca atcccgtgta gaattaagca gatcatcaat
     6601 atgtggcaag gggtcggccg agccatatat gctccgccta tagcgggcaa tatcacctgc
     6661 aaatccaaca taacgggtct tcttcttgta cgcgacggtg gagaaacaaa cgataccaat
     6721 accaccgaga catttagacc aggcggagga gatatgcgag ataattggcg gagcgaattg
     6781 tacaagtaca aggtggtcga gataaagcct ttgggtgtgg cacccacggg cgcaaagcgc
     6841 agaatagtcg gaagagaaaa gagagcagtt gggctcggcg ctgcttttct tggtttcctc
     6901 agcgttgccg gtagtacgat gggagcagcg tcaatgaccc tgactgtcca agcgcgacag
     6961 taccttagcg gaatagtgca acaacagagc aacctgctgc gggcaattga agcacagcag
     7021 catttgctcc aactgactgt ctggggtata aagcaactgc aggccagggt tttggccata
     7081 gagagatacc tcaaagatca acagctcctg ggtatctggg ggtgttcagg aaagctcatc
     7141 tgtacgacca cagtaccctg gaactcttcc tggagcaata aaaaccttac tgagatctgg
     7201 gataatatga cttggatgca gtgggacaag gaaatctcca actacaccaa tacgatttat
     7261 tggttgcttg aggagtcaca gaatcagcaa gagaagaacg aaaaggatct cctcgccctc
     7321 gattcatgga acaatttgtg gaactggttc gatatttcta aatggctgtg gtacatcaag
     7381 atattcataa tgatcgtcgg cggacttatc ggtctccgaa tcatatttgc ggtactcagt
     7441 atcgtgaaca gggtacggca agggtacagt ccccttagct ttcaaacact taccccgaac
     7501 cctggcggac ctgacaggct tggacgaatc gaggaggaag gaggagaaca agatcgccgc
     7561 aggtctatta gactggtatc cggcttcctc tgataactcg aggccggcaa ggccggatcc
     7621 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     7681 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     7741 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg
     7801 ggaggttttt taaagcaagt aaaacctcta caaatgtggt atggctgatt atgatccggc
     7861 tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
     7921 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     7981 ggtgttggcg ggtgtcgggg cgcagccatg aggtcgactc tagaggatcg atccccgccc
     8041 cggacgaact aaacctgact acgacatctc tgccccttct tcgcggggca gtgcatgtaa
     8101 tcccttcagt tggttggtac aacttgccaa ctgggccctg ttccacatgt gacacggggg
     8161 gggaccaaac acaaaggggt tctctgactg tagttgacat ccttataaat ggatgtgcac
     8221 atttgccaac actgagtggc tttcatcctg gagcagactt tgcagtctgt ggactgcaac
     8281 acaacattgc ctttatgtgt aactcttggc tgaagctctt acaccaatgc tgggggacat
     8341 gtacctccca ggggcccagg aagactacgg gaggctacac caacgtcaat cagaggggcc
     8401 tgtgtagcta ccgataagcg gaccctcaag agggcattag caatagtgtt tataaggccc
     8461 ccttgttaac cctaaacggg tagcatatgc ttcccgggta gtagtatata ctatccagac
     8521 taaccctaat tcaatagcat atgttaccca acgggaagca tatgctatcg aattagggtt
     8581 agtaaaaggg tcctaaggaa cagcgatatc tcccacccca tgagctgtca cggttttatt
     8641 tacatggggt caggattcca cgagggtagt gaaccatttt agtcacaagg gcagtggctg
     8701 aagatcaagg agcgggcagt gaactctcct gaatcttcgc ctgcttcttc attctccttc
     8761 gtttagctaa tagaataact gctgagttgt gaacagtaag gtgtatgtga ggtgctcgaa
     8821 aacaaggttt caggtgacgc ccccagaata aaatttggac ggggggttca gtggtggcat
     8881 tgtgctatga caccaatata accctcacaa accccttggg caataaatac tagtgtagga
     8941 atgaaacatt ctgaatatct ttaacaatag aaatccatgg ggtggggaca agccgtaaag
     9001 actggatgtc catctcacac gaatttatgg ctatgggcaa cacataatcc tagtgcaata
     9061 tgatactggg gttattaaga tgtgtcccag gcagggacca agacaggtga accatgttgt
     9121 tacactctat ttgtaacaag gggaaagaga gtggacgccg acagcagcgg actccactgg
     9181 ttgtctctaa cacccccgaa aattaaacgg ggctccacgc caatggggcc cataaacaaa
     9241 gacaagtggc cactcttttt tttgaaattg tggagtgggg gcacgcgtca gcccccacac
     9301 gccgccctgc ggttttggac tgtaaaataa gggtgtaata acttggctga ttgtaacccc
     9361 gctaaccact gcggtcaaac cacttgccca caaaaccact aatggcaccc cggggaatac
     9421 ctgcataagt aggtgggcgg gccaagatag gggcgcgatt gctgcgatct ggaggacaaa
     9481 ttacacacac ttgcgcctga gcgccaagca cagggttgtt ggtcctcata ttcacgaggt
     9541 cgctgagagc acggtgggct aatgttgcca tgggtagcat atactaccca aatatctgga
     9601 tagcatatgc tatcctaatc tatatctggg tagcataggc tatcctaatc tatatctggg
     9661 tagcatatgc tatcctaatc tatatctggg tagtatatgc tatcctaatt tatatctggg
     9721 tagcataggc tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg
     9781 tagtatatgc tatcctaatc tgtatccggg tagcatatgc tatcctaata gagattaggg
     9841 tagtatatgc tatcctaatt tatatctggg tagcatatac tacccaaata tctggatagc
     9901 atatgctatc ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagc
     9961 ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt
    10021 atatgctatc ctaatttata tctgggtagc ataggctatc ctaatctata tctgggtagc
    10081 atatgctatc ctaatctata tctgggtagt atatgctatc ctaatctgta tccgggtagc
    10141 atatgctatc ctcatgcata tacagtcagc atatgatacc cagtagtaga gtgggagtgc
    10201 tatcctttgc atatgccgcc acctcccaag ggggcgtgaa ttttcgctgc ttgtcctttt
    10261 cctgctggtt gctcccattc ttaggtgaat ttaaggaggc caggctaaag ccgtcgcatg
    10321 tctgattgct caccaggtaa atgtcgctaa tgttttccaa cgcgagaagg tgttgagcgc
    10381 ggagctgagt gacgtgacaa catgggtatg cccaattgcc ccatgttggg aggacgaaaa
    10441 tggtgacaag acagatggcc agaaatacac caacagcacg catgatgtct actggggatt
    10501 tattctttag tgcgggggaa tacacggctt ttaatacgat tgagggcgtc tcctaacaag
    10561 ttacatcact cctgcccttc ctcaccctca tctccatcac ctccttcatc tccgtcatct
    10621 ccgtcatcac cctccgcggc agccccttcc accataggtg gaaaccaggg aggcaaatct
    10681 actccatcgt caaagctgca cacagtcacc ctgatattgc aggtaggagc gggctttgtc
    10741 ataacaaggt ccttaatcgc atccttcaaa acctcagcaa atatatgagt ttgtaaaaag
    10801 accatgaaat aacagacaat ggactccctt agcgggccag gttgtgggcc gggtccaggg
    10861 gccattccaa aggggagacg actcaatggt gtaagacgac attgtggaat agcaagggca
    10921 gttcctcgcc ttaggttgta aagggaggtc ttactacctc catatacgaa cacaccggcg
    10981 acccaagttc cttcgtcggt agtcctttct acgtgactcc tagccaggag agctcttaaa
    11041 ccttctgcaa tgttctcaaa tttcgggttg gaacctcctt gaccacgatg ctt
//
