LOCUS       sequence-240437-       11251 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the AD8 HIV-1 isolate; 
            C-terminal truncation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11251)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 11251)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-AD8gp160-754*
FEATURES             Location/Qualifiers
     source          1..11251
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(651..669)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        737..841
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(842..910,911..1702)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1060..1079)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1873..2461
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2362..2381
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2892..3037
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3080..4117
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4115..4134)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4159..4207
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4861..5240
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5241..5444
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5395..5415
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5441..5465
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             7440..7484
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     polyA_signal    7762..7896
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7786..7805)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7840..7859
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(8039..8061)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8621..10410
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 tctccggaca ccatctctat gtcttggccc tgatcctgag ccgcccgggg ctcctggtct
       61 tccgcctcct cgtcctcgtc ctcttccccg tcctcgtcca tggttatcac cccctcttct
      121 ttgaggtcca ctgccgccgg agccttctgg tccagatgtg tctcccttct ctcctaggcc
      181 atttccaggt cctgtacctg gcccctcgtc agacatgatt cacactaaaa gagatcaata
      241 gacatcttta ttagacgacg ctcagtgaat acagggagtg cagactcctg ccccctccaa
      301 cagccccccc accctcatcc ccttcatggt cgctgtcaga cagatccagg tctgaaaatt
      361 ccccatcctc cgaaccatcc tcgtcctcat caccaattac tcgcagcccg gaaaactccc
      421 gctgaacatc ctcaagattt gcgtcctgag cctcaagcca ggcctcaaat tcctcgtccc
      481 cctttttgct ggacggtagg gatggggatt ctcgggaccc ctcctcttcc tcttcaaggt
      541 caccagacag agatgctact ggggcaacgg aagaaaagct gggtgcggcc tgtgaggatc
      601 agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat
      661 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
      721 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
      781 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
      841 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
      901 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
      961 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     1021 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     1081 ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     1141 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     1201 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     1261 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     1321 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     1381 gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     1441 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     1501 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     1561 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     1621 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     1681 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     1741 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     1801 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     1861 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     1921 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     1981 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     2041 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     2101 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     2161 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     2221 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     2281 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     2341 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     2401 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     2461 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt gaagctgtcc
     2521 ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc
     2581 gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag
     2641 caagacgtag cccagcgcgt cggccccgag atgcgccgcg tgcggctgct ggagatggcg
     2701 gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat
     2761 tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccc tgcttcatcc
     2821 ccgtggcccg ttgctcgcgt ttgctggcgg tgtccccgga agaaatatat ttgcatgtct
     2881 ttagttctat gatgacacaa accccgccca gcgtcttgtc attggcgaat tcgaacacgc
     2941 agatgcagtc ggggcggcgc ggtccgaggt ccacttcgca tattaaggtg acgcgtgtgg
     3001 cctcgaacac cgagcgaccc tgcagcgacc cgcttaacag cgtcaacagc gtgccgcaga
     3061 tcccgggggg caatgagata tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt
     3121 tctgatcgaa aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc
     3181 tcgtgctttc agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc
     3241 cgatggtttc tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat
     3301 tccggaagtg cttgacattg gggaattcag cgagagcctg acctattgca tctcccgccg
     3361 tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctgcagcc
     3421 ggtcgcggag gccatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg
     3481 cccattcgga ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat
     3541 tgctgatccc catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt
     3601 cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct
     3661 cgtgcacgcg gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt
     3721 cattgactgg agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt
     3781 ctggaggccg tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc
     3841 ggagcttgca ggatcgccgc ggctccgggc gtatatgctc cgcattggtc ttgaccaact
     3901 ctatcagagc ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga
     3961 cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc
     4021 ggccgtctgg accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag
     4081 cactcgtccg gatcgggaga tgggggaggc taactgaaac acggaaggag acaataccgg
     4141 aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggg tgttgggtcg
     4201 tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga
     4261 ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc cccaagttcg
     4321 ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat agccactggc
     4381 cccgtgggtt agggacgggg tcccccatgg ggaatggttt atggttcgtg ggggttatta
     4441 ttttgggcgt tgcgtggggt caggtccacg actggactga gcagacagac ccatggtttt
     4501 tggatggcct gggcatggac cgcatgtact ggcgcgacac gaacaccggg cgtctgtggc
     4561 tgccaaacac ccccgacccc caaaaaccac cgcgcggatt tctggcgtgc caagctagtc
     4621 gaccaattct catgtttgac agcttatcat cgcagatccg ggcaacgttg ttgccattgc
     4681 tgcaggcgca gaactggtag gtatggaaga tctatacatt gaatcaatat tggcaattag
     4741 ccatattagt cattggttat atagcataaa tcaatattgg ctattggcca ttgcatacgt
     4801 tgtatctata tcataatatg tacatttata ttggctcatg tccaatatga ccgccatgtt
     4861 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
     4921 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
     4981 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
     5041 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
     5101 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
     5161 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
     5221 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
     5281 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
     5341 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
     5401 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
     5461 agatctctag aagctgggta ccagctgcta gcgccaccat gccaatgggt tcactgcaac
     5521 cacttgcgac gctgtacctc ttgggcatgc tggtggcatc cgttctggca gttgagaatc
     5581 tctgggtcac tgtttactac ggcgtgcccg tatggaagga agccacgacg acattgttct
     5641 gtgcgagcga tgcgaaggca tacgacactg aggtccacaa tgtctgggca acacacgcgt
     5701 gcgtccctac cgaccccaat ccccaggaag tggtccttga aaatgtcact gagaatttta
     5761 atatgtggaa gaataatatg gttgagcaga tgcatgagga catcatttct ctgtgggatc
     5821 agtccctcaa accttgtgtc aagttgacgc ctctgtgtgt aaccctcaac tgcaccgatt
     5881 tgaggaatgt aaccaatatc aacaattcta gtgaaggtat gaggggagag atcaaaaact
     5941 gctccttcaa cataaccacc tccattcgag acaaggtaaa aaaggattac gctctctttt
     6001 atagattgga cgtggtaccc attgacaatg ataacaccag ttataggctg ataaactgta
     6061 atacctcaac gattacacaa gcttgtccta aagtatcatt cgaacccatt ccgatccatt
     6121 actgtacgcc ggccggtttc gccatcctta aatgtaagga taagaaattc aatggtactg
     6181 gcccgtgcaa aaatgtatct acggtccagt gtacacacgg cataagaccc gtagtatcta
     6241 cgcaactcct gcttaacggg agcctggccg aagaggaagt tgtaatcaga agtagtaact
     6301 tcactgataa tgccaagaac atcattgtgc agttgaaaga gtcagtcgag atcaactgta
     6361 ccaggccgaa caataataca cgaaagtcaa tacacatagg gcccggaagg gcattctaca
     6421 ccactgggga tataatcgga gatatacgcc aagcgcattg caatatcagt aggaccaagt
     6481 ggaacaatac gctgaaccaa atcgctacaa agcttaagga acaatttggg aataacaaga
     6541 caatagtatt taatcaatca tctgggggcg acccggagat agtcatgcat tcatttaatt
     6601 gcggcggaga atttttttat tgtaattcca cacagctttt taatagcaca tggaatttca
     6661 acggaacatg gaatctgaca cagtccaatg gtacggaggg gaatgacaca attacccttc
     6721 cctgtcgcat taaacagata attaatatgt ggcaagaagt cggaaaggcg atgtatgctc
     6781 ctcctattcg agggcagata aggtgctcat caaatatcac cggcctcata ctgactcggg
     6841 acggcggaaa taatcacaac aatgacactg agacatttcg cccaggtgga ggtgatatgc
     6901 gggataactg gcgctccgaa ttgtacaagt acaaagttgt caagattgag ccactcggag
     6961 tggccccgac aaaggctaag aggcgggtcg tacaaaggga gaaacgcgcg gtaggaacca
     7021 ttggagccat gttcttgggt ttcttgggcg cagcgggatc caccatgggg gcggcctcta
     7081 tcacactgac cgtgcaggca agactcctct tgagcggcat cgtgcaacag caaaacaatc
     7141 tcttgcgcgc tattgaggct caacagcacc tgcttcaact taccgtatgg ggcatcaaac
     7201 aattgcaggc tcgggtcttg gctgtcgaga ggtatttgcg ggatcagcaa cttttgggta
     7261 tctggggttg ttccgggaag ctcatctgca caacggctgt cccatggaat gccagttggt
     7321 ccaacaagac cctggacatg atctggaata acatgacttg gatggagtgg gagagagaaa
     7381 tcgacaacta cacgggactc atctatacct tgattgagga gtctcagaac caacaagaga
     7441 agaacgagca ggaactgctg gagttggaca agtgggccag tctgtggaat tggttcgata
     7501 taaccaactg gctttggtac atcaaaatat ttataatgat cgttggtggt ctgattggtc
     7561 ttaggattgt tttcaccgtc ctgagcattg tcaaccgcgt gaggcagggt tattctcccc
     7621 tttcctttca aactcacttg cccgccccta gggggccaga ccgccctgag gggattgaag
     7681 aggagggcgg cgaccgcgac cgagatagga gtgtgcggct cgtggatggg tttctgtgat
     7741 aactcgaggc cggcaaggcc ggatccagac atgataagat acattgatga gtttggacaa
     7801 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
     7861 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt
     7921 atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa
     7981 tgtggtatgg ctgattatga tccggctgcc tcgcgcgttt cggtgatgac ggtgaaaacc
     8041 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
     8101 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaggt
     8161 cgactctaga ggatcgatcc ccgccccgga cgaactaaac ctgactacga catctctgcc
     8221 ccttcttcgc ggggcagtgc atgtaatccc ttcagttggt tggtacaact tgccaactgg
     8281 gccctgttcc acatgtgaca cgggggggga ccaaacacaa aggggttctc tgactgtagt
     8341 tgacatcctt ataaatggat gtgcacattt gccaacactg agtggctttc atcctggagc
     8401 agactttgca gtctgtggac tgcaacacaa cattgccttt atgtgtaact cttggctgaa
     8461 gctcttacac caatgctggg ggacatgtac ctcccagggg cccaggaaga ctacgggagg
     8521 ctacaccaac gtcaatcaga ggggcctgtg tagctaccga taagcggacc ctcaagaggg
     8581 cattagcaat agtgtttata aggccccctt gttaacccta aacgggtagc atatgcttcc
     8641 cgggtagtag tatatactat ccagactaac cctaattcaa tagcatatgt tacccaacgg
     8701 gaagcatatg ctatcgaatt agggttagta aaagggtcct aaggaacagc gatatctccc
     8761 accccatgag ctgtcacggt tttatttaca tggggtcagg attccacgag ggtagtgaac
     8821 cattttagtc acaagggcag tggctgaaga tcaaggagcg ggcagtgaac tctcctgaat
     8881 cttcgcctgc ttcttcattc tccttcgttt agctaataga ataactgctg agttgtgaac
     8941 agtaaggtgt atgtgaggtg ctcgaaaaca aggtttcagg tgacgccccc agaataaaat
     9001 ttggacgggg ggttcagtgg tggcattgtg ctatgacacc aatataaccc tcacaaaccc
     9061 cttgggcaat aaatactagt gtaggaatga aacattctga atatctttaa caatagaaat
     9121 ccatggggtg gggacaagcc gtaaagactg gatgtccatc tcacacgaat ttatggctat
     9181 gggcaacaca taatcctagt gcaatatgat actggggtta ttaagatgtg tcccaggcag
     9241 ggaccaagac aggtgaacca tgttgttaca ctctatttgt aacaagggga aagagagtgg
     9301 acgccgacag cagcggactc cactggttgt ctctaacacc cccgaaaatt aaacggggct
     9361 ccacgccaat ggggcccata aacaaagaca agtggccact cttttttttg aaattgtgga
     9421 gtgggggcac gcgtcagccc ccacacgccg ccctgcggtt ttggactgta aaataagggt
     9481 gtaataactt ggctgattgt aaccccgcta accactgcgg tcaaaccact tgcccacaaa
     9541 accactaatg gcaccccggg gaatacctgc ataagtaggt gggcgggcca agataggggc
     9601 gcgattgctg cgatctggag gacaaattac acacacttgc gcctgagcgc caagcacagg
     9661 gttgttggtc ctcatattca cgaggtcgct gagagcacgg tgggctaatg ttgccatggg
     9721 tagcatatac tacccaaata tctggatagc atatgctatc ctaatctata tctgggtagc
     9781 ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt
     9841 atatgctatc ctaatttata tctgggtagc ataggctatc ctaatctata tctgggtagc
     9901 atatgctatc ctaatctata tctgggtagt atatgctatc ctaatctgta tccgggtagc
     9961 atatgctatc ctaatagaga ttagggtagt atatgctatc ctaatttata tctgggtagc
    10021 atatactacc caaatatctg gatagcatat gctatcctaa tctatatctg ggtagcatat
    10081 gctatcctaa tctatatctg ggtagcatag gctatcctaa tctatatctg ggtagcatat
    10141 gctatcctaa tctatatctg ggtagtatat gctatcctaa tttatatctg ggtagcatag
    10201 gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagtatat
    10261 gctatcctaa tctgtatccg ggtagcatat gctatcctca tgcatataca gtcagcatat
    10321 gatacccagt agtagagtgg gagtgctatc ctttgcatat gccgccacct cccaaggggg
    10381 cgtgaatttt cgctgcttgt ccttttcctg ctggttgctc ccattcttag gtgaatttaa
    10441 ggaggccagg ctaaagccgt cgcatgtctg attgctcacc aggtaaatgt cgctaatgtt
    10501 ttccaacgcg agaaggtgtt gagcgcggag ctgagtgacg tgacaacatg ggtatgccca
    10561 attgccccat gttgggagga cgaaaatggt gacaagacag atggccagaa atacaccaac
    10621 agcacgcatg atgtctactg gggatttatt ctttagtgcg ggggaataca cggcttttaa
    10681 tacgattgag ggcgtctcct aacaagttac atcactcctg cccttcctca ccctcatctc
    10741 catcacctcc ttcatctccg tcatctccgt catcaccctc cgcggcagcc ccttccacca
    10801 taggtggaaa ccagggaggc aaatctactc catcgtcaaa gctgcacaca gtcaccctga
    10861 tattgcaggt aggagcgggc tttgtcataa caaggtcctt aatcgcatcc ttcaaaacct
    10921 cagcaaatat atgagtttgt aaaaagacca tgaaataaca gacaatggac tcccttagcg
    10981 ggccaggttg tgggccgggt ccaggggcca ttccaaaggg gagacgactc aatggtgtaa
    11041 gacgacattg tggaatagca agggcagttc ctcgccttag gttgtaaagg gaggtcttac
    11101 tacctccata tacgaacaca ccggcgaccc aagttccttc gtcggtagtc ctttctacgt
    11161 gactcctagc caggagagct cttaaacctt ctgcaatgtt ctcaaatttc gggttggaac
    11221 ctccttgacc acgatgcttt ccaaaccacc c
//
