LOCUS       sequence-240438-       10055 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the 001428 HIV-1 isolate; 
            C-terminal truncation; QES mutant with enhanced binding to PG16.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10055)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 10055)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-001428-QES.c10.L544Y-754*
FEATURES             Location/Qualifiers
     source          1..10055
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(572..590)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        658..762
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(763..831,832..1623)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(981..1000)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1794..2382
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2283..2302
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2813..2958
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3001..4038
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4036..4055)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4080..4128
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4782..5161
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5162..5365
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5316..5336
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5362..5386
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     polyA_signal    7695..7829
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7719..7738)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7773..7792
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(7972..7994)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 cctcttcccc gtcctcgtcc atggttatca ccccctcttc tttgaggtcc actgccgccg
       61 gagccttctg gtccagatgt gtctcccttc tctcctaggc catttccagg tcctgtacct
      121 ggcccctcgt cagacatgat tcacactaaa agagatcaat agacatcttt attagacgac
      181 gctcagtgaa tacagggagt gcagactcct gccccctcca acagcccccc caccctcatc
      241 cccttcatgg tcgctgtcag acagatccag gtctgaaaat tccccatcct ccgaaccatc
      301 ctcgtcctca tcaccaatta ctcgcagccc ggaaaactcc cgctgaacat cctcaagatt
      361 tgcgtcctga gcctcaagcc aggcctcaaa ttcctcgtcc ccctttttgc tggacggtag
      421 ggatggggat tctcgggacc cctcctcttc ctcttcaagg tcaccagaca gagatgctac
      481 tggggcaacg gaagaaaagc tgggtgcggc ctgtgaggat cagcttatcg atgataagct
      541 gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt
      601 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
      661 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
      721 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
      781 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
      841 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
      901 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
      961 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa
     1021 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     1081 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     1141 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     1201 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     1261 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca
     1321 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     1381 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     1441 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     1501 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     1561 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     1621 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     1681 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     1741 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     1801 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     1861 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     1921 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     1981 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     2041 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     2101 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     2161 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     2221 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     2281 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     2341 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     2401 tttttacggt tcctggcctt ttgctggcct tgaagctgtc cctgatggtc gtcatctacc
     2461 tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc
     2521 ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg
     2581 tcggccccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct
     2641 gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg
     2701 gagtggtgaa tccgttagcg aggtgccgcc ctgcttcatc cccgtggccc gttgctcgcg
     2761 tttgctggcg gtgtccccgg aagaaatata tttgcatgtc tttagttcta tgatgacaca
     2821 aaccccgccc agcgtcttgt cattggcgaa ttcgaacacg cagatgcagt cggggcggcg
     2881 cggtccgagg tccacttcgc atattaaggt gacgcgtgtg gcctcgaaca ccgagcgacc
     2941 ctgcagcgac ccgcttaaca gcgtcaacag cgtgccgcag atcccggggg gcaatgagat
     3001 atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac
     3061 agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat
     3121 gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat
     3181 cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt
     3241 ggggaattca gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg
     3301 caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga ggccatggat
     3361 gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga
     3421 atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat
     3481 cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag
     3541 ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc
     3601 tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg
     3661 atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct
     3721 tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgccg
     3781 cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac
     3841 ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga
     3901 gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc
     3961 tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc ggatcgggag
     4021 atgggggagg ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac
     4081 ggcaataaaa agacagaata aaacgcacgg gtgttgggtc gtttgttcat aaacgcgggg
     4141 ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg ggccaatacg
     4201 cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc ccagggctcg
     4261 cagccaacgt cggggcggca ggccctgcca tagccactgg ccccgtgggt tagggacggg
     4321 gtcccccatg gggaatggtt tatggttcgt gggggttatt attttgggcg ttgcgtgggg
     4381 tcaggtccac gactggactg agcagacaga cccatggttt ttggatggcc tgggcatgga
     4441 ccgcatgtac tggcgcgaca cgaacaccgg gcgtctgtgg ctgccaaaca cccccgaccc
     4501 ccaaaaacca ccgcgcggat ttctggcgtg ccaagctagt cgaccaattc tcatgtttga
     4561 cagcttatca tcgcagatcc gggcaacgtt gttgccattg ctgcaggcgc agaactggta
     4621 ggtatggaag atctatacat tgaatcaata ttggcaatta gccatattag tcattggtta
     4681 tatagcataa atcaatattg gctattggcc attgcatacg ttgtatctat atcataatat
     4741 gtacatttat attggctcat gtccaatatg accgccatgt tgacattgat tattgactag
     4801 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
     4861 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
     4921 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
     4981 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
     5041 tccgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
     5101 gaccttacgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
     5161 ggtgatgcgg ttttggcagt acaccaatgg gcgtggatag cggtttgact cacggggatt
     5221 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
     5281 ctttccaaaa tgtcgtaata accccgcccc gttgacgcaa atgggcggta ggcgtgtacg
     5341 gtgggaggtc tatataagca gagctcgttt agtgaaccgt cagatctcta gaagctgggt
     5401 accagctgct agcgccacca tgcctatggg gagcctgcaa ccgttggcca cgttgtacct
     5461 cctgggcatg ctcgtagcaa gtgtgctggc agtcgagaat ctctgggtaa ccgtttacta
     5521 tggggttccc gtgtggaagg aggcgcgcac aactttgttt tgcgcgtctg atgcgaaggc
     5581 ctacgagacc gaagtccata acgtgtgggc aacccacgct tgcgtaccta cggaccctaa
     5641 cccccaagag atggtgttgg gaaacgtcac ggaaaacttc aacatgtgga agaatgatat
     5701 ggtagaccag atgcacgagg acgtaatttc tttgtgggca caaagtctca agccttgtgt
     5761 taaactgacg ccactttgcg taaccttgga atgcacacag gtcaacgcga ctcaggggaa
     5821 tacaactcag gtaaacgtca ctcaggtcaa cggcgatgaa atgaagaatt gcagtttcaa
     5881 cacaacgacc gaaatcagag acaagaaaca aaaagcgtat gcactgttct atcgactgga
     5941 cctcgttccg ttggagagag agaacagagg agattcaaat tccgcatcta agtacattct
     6001 catcaattgt aacacctctg ccatcacgca ggcgtgccca aaaatgaatt tcgaccctat
     6061 cccaatacat tactgcaccc cggcaggcta tgcaatactc aaatgcaata ataagacatt
     6121 taacggcaca ggttcttgca acaacgttag tacagtgcaa tgcactcacg ggattaagcc
     6181 cactatgagc acacaactgc tgcttaatgg ttcattggcc gaagaggaga ttatcattag
     6241 aagcgaaaac ctcaccgata atgtaaagac catcattgtc cacctggatc agagcgtgga
     6301 gatcgtgtgt actagaccga ataacaacac ccggaagagt atcagaatcg ggccaggcca
     6361 gacattctat gctactggag acataatcgg aaacattcga gaggcccact gcaacatctc
     6421 cgaaaaaaag tggcatgaaa tgctgaggag agtgtctgaa aagcttgcgg agcactttcc
     6481 aaacaagacc atcaaattta cttctagcag tggtggcgac ttggagataa cgactcatag
     6541 ttttaactgc agaggagaat ttttttactg caatacgtct gggcttttca actccacata
     6601 tatgccgaac ggaacatata tgcccaatgg cacgaataac agcaattcca cgataattct
     6661 gccctgtaga ataaagcaaa ttataaatat gtggcaagag gtagggagag cgatgtatgc
     6721 tccaccaata gctggaaaca ttacttgtaa ttctaatata accgggctct tgctcgtgag
     6781 ggacggagga aaaaataaca acactgagat attccgcccc ggcggggggg atatgcggga
     6841 caactggaga agcgaacttt ataagtacaa ggtcgttgag attaaacccc tgggcgtcgc
     6901 cccgacgagg gcgaagcgac gggttgttga gagggaaaaa cgagcggtcg gcctcggcgc
     6961 cgttttcctc ggtttcttgg gggcagctgg atctactatg ggagccgcat caatcacttt
     7021 gacagtgcag gccagacaat acctgtcagg aatcgttcag cagcaatcaa atctcttgca
     7081 agctatcgag gctcaacaac acttgttgca gcttacggtt tgggggatca aacagcttca
     7141 aacccgggta cttgctatcg aaagatattt gaaggaccaa caactcctcg gtatctgggg
     7201 gtgcagtgga aaattgatct gtactactgc cgtgccttgg aattctagtt ggtctaacaa
     7261 aagtctcacg gacatctggg acaatatgac atggatgcag tgggatagag aggtgagcaa
     7321 ctataccgga attatttatc gccttctgga ggactctcag aatcaacaag agaggaacga
     7381 gcaagacctc ctcgcgctcg attcctggaa gaatctttgg tcatggtttg atatctcaaa
     7441 ttggctctgg tatataaaaa ttttcataat gatagtaggt gggttgattg gtcttcgcat
     7501 catttttgcg gtattgtcta tcgtcaaccg agtaagacag gggtattccc cattgtcttt
     7561 ccaaacattg accccaaacc cgactggccc cgacagactc gggagaatcg aagaagaggg
     7621 aggtgagcag gatagagata ggagcgtgag gcttgtgaac ggtttcctgt gataactcga
     7681 ggccggcaag gccggatcca gacatgataa gatacattga tgagtttgga caaaccacaa
     7741 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
     7801 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc
     7861 aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta
     7921 tggctgatta tgatccggct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca
     7981 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
     8041 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga ggtcgactct
     8101 agaggatcga tccccgcccc ggacgaacta aacctgacta cgacatctct gccccttctt
     8161 cgcggggcag tgcatgtaat cccttcagtt ggttggtaca acttgccaac tgggccctgt
     8221 tccacatgtg acacgggggg ggaccaaaca caaaggggtt ctctgactgt agttgacatc
     8281 cttataaatg gatgtgcaca tttgccaaca ctgagtggct ttcatcctgg agcagacttt
     8341 gcagtctgtg gactgcaaca caacattgcc tttatgtgta actcttggct gaagctctta
     8401 caccaatgct gggggacatg tacctcccag gggcccagga agactacggg aggctacacc
     8461 aacgtcaatc agaggggcct gtgtagctac cgataagcgg accctcaaga gggcattagc
     8521 aatagtgttt ataaggcccc cttgttaacc ctaaacgggt agcatatgct tcccgggtag
     8581 tagtatatac tatccagact aaccctaatt caatagcata tgttacccaa cgggaagcat
     8641 atgctatcga attagggtta gtaaaagggt cctaaggaac agcgatatct cccaccccat
     8701 gagctgtcac ggttttattt acatggggtc aggattccac gagggtagtg aaccatttta
     8761 gtcacaaggg cagtggctga agatcaagga gcgggcagtg aactctcctg aatcttcgcc
     8821 tgcttcttca ttctccttcg tttagctaat agaataactg ctgagttgtg aacagtaagg
     8881 tgtatgtgag gtgctcgaaa acaaggtttc aggtgacgcc cccagaataa aatttggacg
     8941 gggggttcag tggtggcatt gtgctatgac accaatataa ccctcacaaa ccccttgggc
     9001 aataaatact agtgtaggaa tgaaacattc tgaatatctt taacaataga aatccatggg
     9061 gtggggacaa gccgtaaaga ctggatgtcc atctcacacg aatttatggc tatgggcaac
     9121 acataatcct agtgcaatat gatactgggg ttattaagat gtgtcccagg cagggaccaa
     9181 gacaggtgaa ccatgttgtt acactctatt tgtaacaagg ggaaagagag tggacgccga
     9241 cagcagcgga ctccactggt tgtctctaac acccccgaaa attaaacggg gctccacgcc
     9301 aatggggccc ataaacaaag acaagtggcc actctttttt ttgaaattgt ggagtggggg
     9361 cacgcgtcag cccccacacg ccgccctgcg gttttggact gtaaaataag ggtgtaataa
     9421 cttggctgat tgtaaccccg ctaaccactg cggtcaaacc acttgcccac aaaaccacta
     9481 atggcacccc ggggaatacc tgcataagta ggtgggcggg ccaagatagg ggcgcgattg
     9541 ctgcgatctg gaggacaaat tacacacact tgcgcctgag cgccaagcac agggttgttg
     9601 gtcctcatat tcacgaggtc gctgagagca cggtgggcta atgttgccat gggtagcata
     9661 tactacccaa atatctggat agcatatgct atcctaatct atatctgggt agcataggct
     9721 atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct
     9781 atcctaattt atatctgggt agcataggct atcctaatct atatctgggt agcatatgct
     9841 atcctaatct atatctgggt agtatatgct atcctaatct gtatccgggt agcatatgct
     9901 atcctaatag agattagggt agtatatgct atcctaattt atatctgggt agcatatact
     9961 acccaaatat ctggatagca tatgctatcc taatctatat ctgggtagca tatgctatcc
    10021 taatctatat ctgggtagca taggctatcc taatc
//
